Summary of Study ST002981

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001857. The data can be accessed directly via it's Project DOI: 10.21228/M8P99M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002981
Study TitleDevelopmental Neurotoxicity of Deltamethrin Exposure on Hypothalamic Neurogenesis in Embryonic Zebrafish
Study SummaryIn this study, to investigate DM effects at different stages of early life, zebrafish embryos were exposed to DM just before (10-16 hpf), at the onset of (16-24 hpf), at the peak of (24-36 hpf) hypothalamic neurogenesis and across 10-120 hpf with different dosage levels (0, 1, 100, and 250 nM).
Institute
College of Marine Food and Biological Engineering, Jimei University
Last NameGao
First NameLonghua
AddressNo. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, China
Email1932076629@qq.com
Phone18718180398
Submit Date2023-11-12
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-11-22
Release Version1
Longhua Gao Longhua Gao
https://dx.doi.org/10.21228/M8P99M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001857
Project DOI:doi: 10.21228/M8P99M
Project Title:Developmental Neurotoxicity of Deltamethrin Exposure on Hypothalamic Neurogenesis in Embryonic Zebrafish
Project Summary:In this study, to investigate DM effects at different stages of early life, zebrafish embryos were exposed to DM just before (10-16 hpf), at the onset of (16-24 hpf), at the peak of (24-36 hpf) hypothalamic neurogenesis and across 10-120 hpf with different dosage levels (0, 1, 100, and 250 nM).
Institute:College of Marine Food and Biological Engineering, Jimei University
Last Name:Gao
First Name:Longhua
Address:No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, China
Email:1932076629@qq.com
Phone:18718180398

Subject:

Subject ID:SU003094
Subject Type:Fish
Subject Species:Danio rerio
Taxonomy ID:7955

Factors:

Subject type: Fish; Subject species: Danio rerio (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA323431100n-16-24_2100nM
SA323432100n-16-24_3100nM
SA323433100n-16-24_6100nM
SA323434100n-16-24_1100nM
SA323435100n-16-24_5100nM
SA323436100n-10-16_5100nM
SA323437100n-10-16_2100nM
SA323438100n-10-16_3100nM
SA323439100n-10-16_4100nM
SA323440100n-24-36_1100nM
SA323441100n-10-16_6100nM
SA323442100n-24-36_3100nM
SA323443100n-10-5d_3100nM
SA323444100n-10-5d_4100nM
SA323445100n-10-5d_5100nM
SA323446100n-10-5d_6100nM
SA323447100n-10-5d_2100nM
SA323448100n-10-5d_1100nM
SA323449100n-10-16_1100nM
SA323450100n-24-36_4100nM
SA323451100n-24-36_5100nM
SA323452100n-24-36_6100nM
SA323453100n-24-36_2100nM
SA323454100n-16-24_4100nM
SA3234551n-16-24_21nM
SA3234561n-16-24_11nM
SA3234571n-16-24_31nM
SA3234581n-16-24_41nM
SA3234591n-16-24_51nM
SA3234601n-10-16_61nM
SA3234611n-10-16_51nM
SA3234621n-10-5d_21nM
SA3234631n-10-16_21nM
SA3234641n-10-16_31nM
SA3234651n-10-16_41nM
SA3234661n-16-24_61nM
SA3234671n-10-16_11nM
SA3234681n-10-5d_61nM
SA3234691n-24-36_11nM
SA3234701n-10-5d_51nM
SA3234711n-10-5d_41nM
SA3234721n-10-5d_31nM
SA3234731n-24-36_61nM
SA3234741n-10-5d_11nM
SA3234751n-24-36_51nM
SA3234761n-24-36_21nM
SA3234771n-24-36_31nM
SA3234781n-24-36_41nM
SA323479250n-24-36_2250nM
SA323480250n-24-36_1250nM
SA323481250n-16-24_5250nM
SA323482250n-16-24_6250nM
SA323483250n-16-24_4250nM
SA323484250n-10-5d_1250nM
SA323485250n-16-24_3250nM
SA323486250n-10-5d_2250nM
SA323487250n-24-36_6250nM
SA323488250n-24-36_5250nM
SA323489250n-24-36_4250nM
SA323490250n-24-36_3250nM
SA323491250n-10-16_2250nM
SA323492250n-10-5d_4250nM
SA323493250n-10-5d_5250nM
SA323494250n-10-5d_6250nM
SA323495250n-10-5d_3250nM
SA323496250n-10-16_1250nM
SA323497250n-10-16_3250nM
SA323498250n-16-24_1250nM
SA323499250n-10-16_6250nM
SA323500250n-10-16_5250nM
SA323501250n-16-24_2250nM
SA323502250n-10-16_4250nM
SA3235030_2Control
SA3235040_5Control
SA3235050_1Control
SA3235060_6Control
SA3235070_4Control
SA3235080_3Control
Showing results 1 to 78 of 78

Collection:

Collection ID:CO003087
Collection Summary:Wild-type zebrafish embryos (AB line) were maintained in fish water (0.2 % Instant Ocean Salt in deionized water, pH 6.5-8.5, conductivity 450-550 μS.cm-1 and hardness 50-100 mg·L-1 CaCO3) on a 14-h light:10-h dark cycle at 28 °C. Thirty-three embryos were placed in each well of a 6-well plate (Nest Biotech., China) in 3 mL fresh fish water. Zebrafish embryos were exposed to DM from 10-16, 16-24, 24-36 and 10-120 hpf, respectively. DM was washed out after each restricted time point, and zebrafish were assayed at the larval stage, 120 hpf. At each exposure stage, zebrafish embryos were given DM dosages of 1, 100, and 250 nM. The control group received an equivalent volume of dimethyl sulfoxide (DMSO). In each group, 3 wells of zebrafish were utilized to evaluate developmental toxicity, locomotor behavior and apoptotic cells in the central nervous system, while 6 wells of zebrafish were created as separate biological replicates for subsequent lipidomics analysis and kept immediately at -80 °C.
Sample Type:Zebrafish

Treatment:

Treatment ID:TR003103
Treatment Summary:Wild-type zebrafish embryos (AB line) were maintained in fish water (0.2 % Instant Ocean Salt in deionized water, pH 6.5-8.5, conductivity 450-550 μS.cm-1 and hardness 50-100 mg·L-1 CaCO3) on a 14-h light:10-h dark cycle at 28 °C. Thirty-three embryos were placed in each well of a 6-well plate (Nest Biotech., China) in 3 mL fresh fish water. Zebrafish embryos were exposed to DM from 10-16, 16-24, 24-36 and 10-120 hpf, respectively. DM was washed out after each restricted time point, and zebrafish were assayed at the larval stage, 120 hpf. At each exposure stage, zebrafish embryos were given DM dosages of 1, 100, and 250 nM. The control group received an equivalent volume of dimethyl sulfoxide (DMSO). In each group, 3 wells of zebrafish were utilized to evaluate developmental toxicity, locomotor behavior and apoptotic cells in the central nervous system, while 6 wells of zebrafish were created as separate biological replicates for subsequent lipidomics analysis and kept immediately at -80 °C.

Sample Preparation:

Sampleprep ID:SP003100
Sampleprep Summary:Each replicate of zebrafish (1 well, ~10 mg) was accurately weighted and transferred to an Eppendorf tube. Then, each sample was spiked with 400 μL of precooled methanol containing lipid standards (i.e., 1.5 μg/mL of phosphatidylcholine (PC) (19:0/19:0), 1.5 μg/mL of phosphatidylethanolamine (PE) (15:0/15:0), 1.5 μg/mL of lysophospatidylcholine (LPC) (19:0), 1.5 μg/mL of sphingomyelin (SM) (d18:1/12:0), 1.2 μg/mL of triacylglycerol (TG) (15:0/15:0/15:0), 1.2 μg/mL of ceramide (Cer) (d18:1/17:0), 1 μg/mL of palmitic acid (fatty acid (FA) (16:0))-d3, and 1 μg/mL of stearic acid (FA(18:0))-d3), followed by tissue homogenization using the bead-based homogenizer (Tissuelyser-24, Shanghai jingxin industrial development co., ltd, China) for 1 min at 65 Hz. Next, 1 mL of tert-butyl methyl ether (MTBE) was added to the tube, and the mixture was thoroughly vortexed (1,000 rpm, 10 ℃, 30 min). Phase separation was induced by adding 400 μL of Milli-Q water and centrifugation at 15,000 g for 15 min at 6 ℃. The upper hydrophobic phase was collected and vacuum-dried in in a refrigerated CentriVap concentrator (Labconco, USA), followed by storage at -80 °C until subsequent analysis.

Combined analysis:

Analysis ID AN004899 AN004900
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Orbitrap Exploris 120 Thermo Orbitrap Exploris120
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH003696
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
Column Temperature:55
Flow Gradient:First maintain 32% B for 1.5 minutes, then linearly increase from 1.5 to 15.5 minutes to 85% B, then linearly increase to 97% B at 0.1 minutes, maintain for 18 minutes, and then rapidly decrease to 32% B at 0.1 minutes, equilibrate until the next injection
Flow Rate:0.4 mL/min
Solvent A:Acetonitrile/water (6:4, v/v)
Solvent B:Isopropanol/acetonitrile (9:1, v/v)
Chromatography Type:Reversed phase

MS:

MS ID:MS004643
Analysis ID:AN004899
Instrument Name:Thermo Orbitrap Exploris 120
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Xcalibular
Ion Mode:POSITIVE
  
MS ID:MS004644
Analysis ID:AN004900
Instrument Name:Thermo Orbitrap Exploris120
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Xcalibular
Ion Mode:NEGATIVE
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