Summary of Study ST003061
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001907. The data can be accessed directly via it's Project DOI: 10.21228/M8714X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003061 |
| Study Title | Untargeted metabolomics analysis of three tea cultivars. |
| Study Type | leaf tissue |
| Study Summary | To compare flavonoid metabolism in three tea cultivars, namely Camellia sinensis cv. "Jinfenghuang" (JFH), "Fuyun 6" (FY6) and "Bantianyao" (BTY), fresh tea leaves were plucked, rapidly ground to fine powders in liquid nitrogen and subjected to extraction by 70% methanol. The extracts were analyzed by UPLC-Quadrupole Time-of-Flight Mass Spectrometry (UPLC-QTOFMS) using an ACQUITY UPLC system in tandem to a SYNAPT G2-Si QTOF mass spectrometer (Waters, Milford, MA, USA), following the previously described method. Raw data were processed in Progenesis QI (v2.4, Nonlinear Dynamics) with default settings for peak picking and alignment. Metabolites were annotated by searching against our in-house MS/MS spectral library and public databases. |
| Institute | Fujian Agriculture and Forestry University |
| Last Name | Yu |
| First Name | Xiaomin |
| Address | Shangxia Dian Road No. 15, Fuzhou 350002, Fujian, China |
| xmyu0616@sina.com | |
| Phone | (86)18649802306 |
| Submit Date | 2024-01-28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-02-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001907 |
| Project DOI: | doi: 10.21228/M8714X |
| Project Title: | Characterization of CsUGT73AC15 as a Multifunctional Glycosyltransferase Impacting Flavonol Triglycoside Biosynthesis in Tea Plants |
| Project Summary: | Flavonol glycosides, essential for the health benefits and astringent flavors of tea (Camellia sinensis), are abundant in tea leaves as diglycosides and triglycosides. However, the UDP-glycosyltransferases (UGTs) responsible for flavonol multi-glycosylation have remained elusive. In this study, integrated proteomic and metabolomic analyses were performed to identify potential UGTs involved in flavonol triglycoside biosynthesis. Among the tested recombinant enzymes, rCsUGT75AJ1 exhibited flavonoid 4ʹ-O-glucosyltransferase activity, while rCsUGT75L72 catalyzed glucosylation of flavonoids preferentially at the 3-OH position. Remarkably, rCsUGT73AC15 demonstrated substrate promiscuity and regioselectivity, enabling glucosylation of rutin at multiple positions as well as kaempferol 3-O-rutinoside at the 7-OH position. Expression analysis revealed an inverse correlation between the level of CsUGT73AC15 and rutin abundance across different cultivars, suggesting its involvement in flavonol triglycoside biosynthesis in planta. These findings advance the understanding of flavonol triglycoside biosynthesis in tea plants and provide a foundation for further in-vivo characterization of gene functions. |
| Institute: | Fujian Agriculture and Forestry University |
| Last Name: | Yu |
| First Name: | Xiaomin |
| Address: | Shang Xia Dian Road No. 15, Fuzhou, Fujian, 350002, China |
| Email: | xmyu0616@sina.com |
| Phone: | (86)18649802306 |
| Publications: | https://doi.org/10.1021/acs.jafc.4c03824 |
Subject:
| Subject ID: | SU003176 |
| Subject Type: | Plant |
| Subject Species: | Camellia sinensis |
| Taxonomy ID: | 4442 |
Factors:
Subject type: Plant; Subject species: Camellia sinensis (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA331863 | BTY-1 | Bantianyao |
| SA331864 | BTY-2 | Bantianyao |
| SA331865 | BTY-3 | Bantianyao |
| SA331866 | FY6-3 | Fuyun 6 |
| SA331867 | FY6-2 | Fuyun 6 |
| SA331868 | FY6-1 | Fuyun 6 |
| SA331869 | JFH-2 | Jinfenghuang |
| SA331870 | JFH-3 | Jinfenghuang |
| SA331871 | JFH-1 | Jinfenghuang |
| Showing results 1 to 9 of 9 |
Collection:
| Collection ID: | CO003169 |
| Collection Summary: | Mature leaves from the tea cultivars were plucked and flash-frozen in liquid nitrogen. |
| Sample Type: | Leaves |
Treatment:
| Treatment ID: | TR003185 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP003182 |
| Sampleprep Summary: | Leaves were ground into a fine powder using a pre-cooled mortar and pestle. Tea leaf powder (30 mg) was extracted with 1.0 mL of 70% methanol using ultrasonication for 20 min. After centrifugation, the supernatant was filtered through a PVDF filter (0.22 µm, Millipore). |
Combined analysis:
| Analysis ID | AN005016 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity I-Class |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Synapt-G2-Si |
| Ion Mode | NEGATIVE |
| Units | Da |
Chromatography:
| Chromatography ID: | CH003789 |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Gradient: | 1–7% B (0–2 min), 7–40% B (2–13 min), 40–60% B (13–17 min), immediately elevated to 99% B (17 min), held at 99% B (17–22 min) |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004755 |
| Analysis ID: | AN005016 |
| Instrument Name: | Waters Synapt-G2-Si |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MassLynx software (ver 4.1) was used to operate the instrument. Progenesis QI software (ver 2.4) was used for peak picking, alignment and normalization. |
| Ion Mode: | NEGATIVE |
