Summary of Study ST003855
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002412. The data can be accessed directly via it's Project DOI: 10.21228/M8VK0H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003855 |
| Study Title | Mammary gland metabolism and its relevance to the fetoplacental expression of cytokine signaling in Caveolin-1 null mice |
| Study Summary | Mice lacking Caveolin-1 (Cav1), a major protein of the lipid raft of plasma membrane, show dysregulated cellular proliferation of mammary gland and an abnormal fetoplacental communication during pregnancy. The aim of this study is to better understand the functional links of mammary gland metabolism with gene expression of the placenta and fetus. Untargeted metabolomics analysis was performed to examine changes in mammary gland metabolism due to the absence of Cav1. The metabolomics analysis detected a total of 141 metabolites. We identified 81 metabolites that showed differential rank order in the level of expression in the mammary glands of Cav1-null compared to control mice. Differential metabolomics analysis identified 11 metabolites that were significantly changed in the mammary gland due to the absence of Cav1. Integrative metabolomics and transcriptomics analyses were applied to untangle functional links of metabolic pathways of the mammary gland with the gene expression changes of the placenta and fetus. The findings of this study show that metabolism and gene expression of the mammary gland are significantly impacted due to the loss of Cav1. Genes associated with specific metabolic and signaling pathways show coordinated expression changed in the placenta, mammary gland and fetal brain in Cav1-null mice. The cytokine signaling pathway emerges as a key player of the molecular crosstalk among the mammary gland, placenta and fetal brain. By interrogating the single-nuclei gene expression data of placenta and fetal brain previously generated from Cav1-null mice, the study further reveals that these metabolic and signaling genes are differentially regulated in specific cell types of the placenta and fetal brain. The findings of this study expand our understanding about the role of mammary gland metabolism in the regulation of fetoplacental communication in mammalian pregnancy. |
| Institute | Universiy of Missouri, Columbia |
| Last Name | Behura |
| First Name | Susanta |
| Address | 920 East Campus, Columbia, Missouri, 65211, USA |
| behuras@missouri.edu | |
| Phone | 573-882-1722 |
| Submit Date | 2025-03-27 |
| Num Groups | 2 |
| Total Subjects | 6 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2025-04-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002412 |
| Project DOI: | doi: 10.21228/M8VK0H |
| Project Title: | Mammary gland metabolism and its relevance to the fetoplacental expression of cytokine signaling in Caveolin-1 null mice |
| Project Summary: | Mice lacking Caveolin-1 (Cav1), a major protein of the lipid raft of plasma membrane, show dysregulated cellular proliferation of mammary gland and an abnormal fetoplacental communication during pregnancy. The aim of this study is to better understand the functional links of mammary gland metabolism with gene expression of the placenta and fetus. Untargeted metabolomics analysis was performed to examine changes in mammary gland metabolism due to the absence of Cav1. The metabolomics analysis detected a total of 141 metabolites. We identified 81 metabolites that showed differential rank order in the level of expression in the mammary glands of Cav1-null compared to control mice. Differential metabolomics analysis identified 11 metabolites that were significantly changed in the mammary gland due to the absence of Cav1. Integrative metabolomics and transcriptomics analyses were applied to untangle functional links of metabolic pathways of the mammary gland with the gene expression changes of the placenta and fetus. The findings of this study show that metabolism and gene expression of the mammary gland are significantly impacted due to the loss of Cav1. Genes associated with specific metabolic and signaling pathways show coordinated expression changed in the placenta, mammary gland and fetal brain in Cav1-null mice. The cytokine signaling pathway emerges as a key player of the molecular crosstalk among the mammary gland, placenta and fetal brain. By interrogating the single-nuclei gene expression data of placenta and fetal brain previously generated from Cav1-null mice, the study further reveals that these metabolic and signaling genes are differentially regulated in specific cell types of the placenta and fetal brain. The findings of this study expand our understanding about the role of mammary gland metabolism in the regulation of fetoplacental communication in mammalian pregnancy. |
| Institute: | Universiy of Missouri, Columbia |
| Last Name: | Behura |
| First Name: | Susanta |
| Address: | 920 East Campus, Columbia, Missouri, 65211, USA |
| Email: | behuras@missouri.edu |
| Phone: | 573-882-1722 |
Subject:
| Subject ID: | SU003989 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | B6.Cg-Cav1tm1Mls/J (Cav1-null) mice; C57BL/6J (Control) mice |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Genotype | Sample type |
|---|---|---|---|---|
| SA422053 | CVMAM-1 | Mammary gland | B6.Cg-Cav1tm1Mls/J | Cav1-null |
| SA422054 | CVMAM-2 | Mammary gland | B6.Cg-Cav1tm1Mls/J | Cav1-null |
| SA422055 | CVMAM-3 | Mammary gland | B6.Cg-Cav1tm1Mls/J | Cav1-null |
| SA422056 | BLMAM-1 | Mammary gland | C57BL6/J | Control |
| SA422057 | BLMAM-2 | Mammary gland | C57BL6/J | Control |
| SA422058 | BLMAM-3 | Mammary gland | C57BL6/J | Control |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO003982 |
| Collection Summary: | The B6.Cg-Cav1tm1Mls/J (Cav1-null) and control (C57BL/6J) mice were purchased from the Jackson Laboratory (stock numbers: 000664 and 007083 respectively). Adult male and female mice (7 weeks old) were paired in cages to induce pregnancy. The females were checked for vaginal plug. The start of pregnancy (day 1) was considered when a vaginal plug was observed. The control and Cav1-null pregnant mice were euthanized in triplicates on gestation day 15, and the mammary gland samples were dissected from mice of both the groups (n=3 each). |
| Sample Type: | mammary gland |
Treatment:
| Treatment ID: | TR003998 |
| Treatment Summary: | No treatment. Genotypes B6.Cg-Cav1tm1Mls/J (Cav1-null mice) C57BL/6J (Control mice) were compared. |
Sample Preparation:
| Sampleprep ID: | SP003995 |
| Sampleprep Summary: | The samples were processed as per the attached protocol of GC-MS used by the University of Missouri Metabolomics Center that provided the metabolomics service. |
| Sampleprep Protocol Filename: | Protocol_CoreLab.pdf |
Combined analysis:
| Analysis ID | AN006333 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890 |
| Column | J&W Scientific DB-5MS (60m x 0.25mm, 0.25mm) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5973 |
| Ion Mode | POSITIVE |
| Units | uM |
Chromatography:
| Chromatography ID: | CH004804 |
| Methods Filename: | Protocol_CoreLab.pdf |
| Instrument Name: | Agilent 6890 |
| Column Name: | J&W Scientific DB-5MS (60m x 0.25mm, 0.25mm) |
| Column Temperature: | Temperature program: 80C for 2 min, then ramped at 5C min-1 to 315C and held for 12 min |
| Flow Gradient: | - |
| Flow Rate: | 1.0 mL/min |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | GC |
MS:
| MS ID: | MS006034 |
| Analysis ID: | AN006333 |
| Instrument Name: | Agilent 5973 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | The spectral analysis was performed by the AMDIS (Automate Mass-spectral Deconvolution and Identification System), and metabolites were identified using a commercial NIST17 mass spectral library. The abundance of the metabolites was determined by the Metabolomics Ion-Based Data Extraction Algorithm (MET-IDEA). |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Protocol_CoreLab.pdf |
