Summary of Study ST003955

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002478. The data can be accessed directly via it's Project DOI: 10.21228/M8BC2J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003955
Study Title	effects of NO treatment on ripening and senescence of apricot fruits,
Study Summary	To understand the regulatory effects of SNP treatment on ripening and senescence of apricot fruits, extensive targeted metabolome technology was used to detect metabolites in three groups of samples (CK-0d, CK-8d and SNP-8d). A total of 1814 metabolites were detected across all groups, with the top five categories being phenolic acids (19.63%), flavonoids (16.48%), derivatives (10.31%), lipids (8.27%), and terpenoids (7.83%)
Institute	Beijing Academy of Agriculture and Forestry Sciences
Last Name	jiang
First Name	yuanye
Address	Haidian District of Beijing City, Beijing, Beijing, 100000, China
Email	yuanyejiang1@outlook.com
Phone	13805170527
Submit Date	2025-05-27
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2025-07-21
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR002478
Project DOI:	doi: 10.21228/M8BC2J
Project Title:	Determination of metabolites before and after storage of apricot fruits treated with Nitric Oxide (NO)
Project Summary:	To understand the regulatory effects of Single Nucleotide Polymorphism [SNP]（NO donor) treatment on ripening and senescence of apricot fruits, extensive targeted metabolome technology was used to detect metabolites in three groups of samples (CK-0d, CK-8d and SNP-8d). A total of 1814 metabolites were detected across all groups, with the top five categories being phenolic acids (19.63%), flavonoids (16.48%), derivatives (10.31%), lipids (8.27%), and terpenoids (7.83%)
Institute:	Beijing Academy of Agriculture and Forestry Sciences
Last Name:	jiang
First Name:	yuanye
Address:	Haidian District of Beijing City, Beijing, Beijing, 100000, China
Email:	yuanyejiang1@outlook.com
Phone:	13805170527

Subject:

Subject ID:	SU004092
Subject Type:	Plant
Subject Species:	Apricot(Prunus armeniaca)
Taxonomy ID:	36596

Factors:

Subject type: Plant; Subject species: Apricot(Prunus armeniaca) (Factor headings shown in green)

mb_sample_id	local_sample_id	Treatment	Sample source
SA452691	CK-8d-1_P	Control	fruit
SA452692	CK-8d-3_P	Control	fruit
SA452693	CK-8d-3_N	Control	fruit
SA452694	CK-0d-1_P	Control	fruit
SA452695	CK-8d-2_N	Control	fruit
SA452696	CK-0d-1_N	Control	fruit
SA452697	CK-8d-1_N	Control	fruit
SA452698	CK-0d-3_P	Control	fruit
SA452699	CK-0d-3_N	Control	fruit
SA452700	CK-0d-2_P	Control	fruit
SA452701	CK-0d-2_N	Control	fruit
SA452702	CK-8d-2_P	Control	fruit
SA452703	SNP-8d-1_N	SNP Treatment	fruit
SA452704	SNP-8d-1_P	SNP Treatment	fruit
SA452705	SNP-8d-2_N	SNP Treatment	fruit
SA452706	SNP-8d-2_P	SNP Treatment	fruit
SA452707	SNP-8d-3_N	SNP Treatment	fruit
SA452708	SNP-8d-3_P	SNP Treatment	fruit

Showing results 1 to 18 of 18

Showing results 1 to 18 of 18

Collection:

Collection ID:	CO004085
Collection Summary:	The apricot cultivar used in the experiment was “Chuanzhihong” (Prunus armeniaca L.), a naturally hybridized cultivar exhibiting mid-April flowering, late-July maturation (95-day fruit development period). Harvested at Beijing Huahai Horticultural Planting Cooperative, Yanqing District, Beijing (40°58 N, 116°19 E). Fruits at commercial maturity were selected based on uniform size and color, along with the absence of mechanical damage or microbial infection. Apricot fruits were transported to the laboratory in foam boxes with ice bags for subsequent treatment. A total of 600 fruits were randomly divided into two groups: the control group was soaked in 10 L distilled water for 10 mins, while the treatment group was soaked in 10 L 0.2 mmol/L SNP solution. After air-drying, fruits were packed into plastic containers (12 fruits per box) and stored at 25°C and 80% relative humidity for 8 days. Sampled tissues were snap-frozen in liquid nitrogen, with residual materials stored at -80°C for subsequent analyses.
Sample Type:	Apricot fruit

Treatment:

Treatment ID:	TR004101
Treatment Summary:	For treatment, in mid-July 2023, a total of 40 kg of apricot fruits harvested at Beijing Huahai Horticultural Planting Cooperative in Yanqing District (40°58′N, 116°19′E) were transported to the laboratory in foam boxes with ice bags for subsequent treatment. A total of 600 fruits were randomly divided into two groups: the control group was soaked in 10 L distilled water for 10 mins, while the treatment group was soaked in 10 L 0.2 mmol/L SNP solution. After air-drying, fruits were packed into plastic containers (12 fruits per box) and stored at 25°C and 80% relative humidity for 8 days. Sampling occurred at 2-day intervals (0, 2, 4, 6, and 8 days) for quality assessment and photographic documentation. Sampled tissues were snap-frozen in liquid nitrogen, with residual materials stored at -80°C for subsequent analyses.

Treatment ID:

TR004101

Treatment Summary:

For treatment, in mid-July 2023, a total of 40 kg of apricot fruits harvested at Beijing Huahai Horticultural Planting Cooperative in Yanqing District (40°58′N, 116°19′E) were transported to the laboratory in foam boxes with ice bags for subsequent treatment. A total of 600 fruits were randomly divided into two groups: the control group was soaked in 10 L distilled water for 10 mins, while the treatment group was soaked in 10 L 0.2 mmol/L SNP solution. After air-drying, fruits were packed into plastic containers (12 fruits per box) and stored at 25°C and 80% relative humidity for 8 days. Sampling occurred at 2-day intervals (0, 2, 4, 6, and 8 days) for quality assessment and photographic documentation. Sampled tissues were snap-frozen in liquid nitrogen, with residual materials stored at -80°C for subsequent analyses.

Sample Preparation:

Sampleprep ID:	SP004098
Sampleprep Summary:	The biological samples were placed in a freeze dryer (Scientz-100F) for vacuum freeze-drying. Then, they were ground into powder using a grinder (MM 400, Retsch) at 30 Hz for 1.5 minutes. Next, 50 mg of sample powder (if less than 50 mg, the extractant was added at a ratio of 1200 μL per 50 mg sample) was weighed using an electronic balance (MS105DΜ) and mixed with 1200 μL of pre-cooled (–20 °C) 70% methanol-water internal standard extraction solution. The mixture was vortexed for 30 seconds every 30 minutes, totaling 6 times. After centrifugation at 12000 rpm for 3 minutes, the supernatant was aspirated, filtered through a microporous membrane (0.22 μm pore size), and stored in a sample vial for UPLC-MS/MS analysis.

Sampleprep ID:

SP004098

Sampleprep Summary:

The biological samples were placed in a freeze dryer (Scientz-100F) for vacuum freeze-drying. Then, they were ground into powder using a grinder (MM 400, Retsch) at 30 Hz for 1.5 minutes. Next, 50 mg of sample powder (if less than 50 mg, the extractant was added at a ratio of 1200 μL per 50 mg sample) was weighed using an electronic balance (MS105DΜ) and mixed with 1200 μL of pre-cooled (–20 °C) 70% methanol-water internal standard extraction solution. The mixture was vortexed for 30 seconds every 30 minutes, totaling 6 times. After centrifugation at 12000 rpm for 3 minutes, the supernatant was aspirated, filtered through a microporous membrane (0.22 μm pore size), and stored in a sample vial for UPLC-MS/MS analysis.

Chromatography:

Chromatography ID:	CH004938
Instrument Name:	Agilent 1200
Column Name:	Waters ACQUITY UPLC BEH C18 (30 x 2.1 mm, 1.7 μm)
Column Temperature:	40℃
Flow Gradient:	At 0.00 min, 95% A and 5% B. Within 9.00 min, the proportion of B linearly increases to 95% (with A decreasing to 5%), and maintains at 95% B (5% A) for 1 min. During 10.00–11.10 min, the proportion of B decreases to 5% (phase A reverting to 95%), and remains balanced at 95% A and 5% B until 14 min.
Flow Rate:	0.35 mL/min
Solvent A:	100% Water; 0.1% Formic acid
Solvent B:	100% Acetonitrile; 0.1% Formic acid
Chromatography Type:	HILIC

Analysis:

Analysis ID:	AN006501
Analysis Type:	MS
Chromatography ID:	CH004938
Num Factors:	2
Num Metabolites:	962
Units:	peak area

Analysis ID:	AN006502
Analysis Type:	MS
Chromatography ID:	CH004938
Num Factors:	2
Num Metabolites:	852
Units:	peak area