Data for (Study ST003728)
(Analysis AN006115)Values for each metabolite have been scaled by dividing by the mean across all factors Run Hierarchial cluster analysis on this study | Run Heatmap cluster analysis on this study| Metabolite | F1 | F2 | F3 | F4 | F5 | F6 | F7 |
|---|---|---|---|---|---|---|---|
| 1,2^-O-dimethyladenosine | NA | NA | 1.4514 | NA | NA | NA | 0.5486 |
| 1-methyladenosine | NA | NA | 1.0257 | NA | NA | NA | 0.9743 |
| 1-methylguanosine | NA | NA | 0.9052 | NA | NA | NA | 1.0948 |
| 1-methylinosine | NA | NA | 1.0217 | NA | NA | NA | 0.9783 |
| 1-methylpseudouridine_1 | NA | NA | 1.0801 | NA | NA | NA | 0.9199 |
| 1-methylpseudouridine_2 | NA | NA | 1.3187 | NA | NA | NA | 0.6813 |
| 2,8-dimethyladenosine | NA | NA | 1.1176 | NA | NA | NA | 0.8824 |
| 2-methyladenosine | NA | NA | 1.1704 | NA | NA | NA | 0.8296 |
| 2-methylthio-N6-isopentenyladenosine_1 | NA | NA | 0.9412 | NA | NA | NA | 1.0588 |
| 2-methylthio-N6-isopentenyladenosine_2 | NA | NA | 1.3050 | NA | NA | NA | 0.6950 |
| 2-methylthio-N6-methyladenosine | NA | NA | 1.2195 | NA | NA | NA | 0.7805 |
| 2^-O-methyladenosine | NA | NA | 1.0343 | NA | NA | NA | 0.9657 |
| 2^-O-methylcytidine | NA | NA | 1.0089 | NA | NA | NA | 0.9911 |
| 2^-O-methylguanosine | NA | NA | 1.0094 | NA | NA | NA | 0.9906 |
| 2^-O-methylinosine_1 | NA | NA | 1.1660 | NA | NA | NA | 0.8340 |
| 2^-O-methylinosine_2 | NA | NA | 1.2334 | NA | NA | NA | 0.6498 |
| 2^-O-methyluridine | NA | NA | 1.0041 | NA | NA | NA | 0.9959 |
| 2-thio-2^-O-methyluridine | NA | NA | 1.0271 | NA | NA | NA | 0.9594 |
| 2-thiocytidine | NA | NA | 1.0074 | NA | NA | NA | 0.9926 |
| 2-thiouridine | NA | NA | 1.1237 | NA | NA | NA | 0.8763 |
| 3,2^-O-dimethyluridine | NA | NA | 0.6349 | NA | NA | NA | 1.3651 |
| 3-(3-amino-3-carboxypropyl)uridine | NA | NA | 1.5122 | NA | NA | NA | 0.4878 |
| 3-methylcytidine | NA | NA | 0.6296 | NA | NA | NA | 1.3704 |
| 3-methylpseudouridine_1 | NA | NA | 0.9537 | NA | NA | NA | 1.0463 |
| 3-methylpseudouridine_2 | NA | NA | 1.2225 | NA | NA | NA | 0.7775 |
| 3-methyluridine | NA | NA | 0.9229 | NA | NA | NA | 1.0771 |
| 4-demethylwyosine | NA | NA | 1.4615 | NA | NA | NA | 0.5385 |
| 4-thiouridine | NA | NA | 1.1150 | NA | NA | NA | 0.8850 |
| 5,2^-O-dimethylcytidine | NA | NA | 0.8945 | NA | NA | NA | 1.1055 |
| 5,2^-O-dimethyluridine | NA | NA | 1.2275 | NA | NA | NA | 0.7725 |
| 5-carbamoylmethyluridine_1 | NA | NA | 0.9417 | NA | NA | NA | 1.0583 |
| 5-carbamoylmethyluridine_2 | NA | NA | 0.9646 | NA | NA | NA | 1.0354 |
| 5-carboxycytidine | NA | NA | 1.0149 | NA | NA | NA | 0.9851 |
| 5-carboxymethylaminomethyluridine_1 | NA | NA | 0.9034 | NA | NA | NA | 1.0966 |
| 5-carboxymethylaminomethyluridine_2 | NA | NA | 0.6140 | NA | NA | NA | 1.2573 |
| 5-carboxymethylaminomethyluridine_3 | NA | NA | 1.3155 | NA | NA | NA | 0.6845 |
| 5-carboxymethyluridine_1 | NA | NA | 1.4538 | NA | NA | NA | 0.5462 |
| 5-carboxymethyluridine_2 | NA | NA | 0.8911 | NA | NA | NA | 1.1089 |
| 5-formylcytidine | NA | NA | 0.9334 | NA | NA | NA | 1.0666 |
| 5-hydroxymethylcytidine | NA | NA | 1.2850 | NA | NA | NA | 0.7150 |
| 5-hydroxyuridine | NA | NA | 1.0576 | NA | NA | NA | 0.9424 |
| 5-methoxycarbonylmethyl-2^-O-methyluridine | NA | NA | 1.3876 | NA | NA | NA | 0.6124 |
| 5-methoxycarbonylmethyl-2-thiouridine_1 | NA | NA | 0.8451 | NA | NA | NA | 1.1549 |
| 5-methoxycarbonylmethyl-2-thiouridine_2 | NA | NA | 1.0885 | NA | NA | NA | 0.9115 |
| 5-methoxycarbonylmethyluridine_1 | NA | NA | 1.6067 | NA | NA | NA | 0.3933 |
| 5-methoxycarbonylmethyluridine_2 | NA | NA | 1.2036 | NA | NA | NA | 0.7964 |
| 5-methoxyuridine | NA | NA | 1.1694 | NA | NA | NA | 0.8306 |
| 5-methyl-2-thiouridine | NA | NA | 1.0250 | NA | NA | NA | 0.9750 |
| 5-methylaminomethyl-2-thiouridine_1 | NA | NA | 1.5832 | NA | NA | NA | 0.4168 |
| 5-methylaminomethyl-2-thiouridine_2 | NA | NA | 0.9454 | NA | NA | NA | 1.0546 |
| 5-methylcytidine | NA | NA | 0.9939 | NA | NA | NA | 1.0061 |
| 5-methyldihydrouridine | NA | NA | 1.5404 | NA | NA | NA | 0.4596 |
| 5-methyluridine | NA | NA | 0.8900 | NA | NA | NA | 1.1100 |
| 7-methylguanosine | NA | NA | 0.0909 | NA | NA | NA | 1.9091 |
| 8-methyladenosine | NA | NA | 0.7061 | NA | NA | NA | 1.2939 |
| adenosine | NA | NA | 1.0000 | NA | NA | NA | 1.0000 |
| cytidine | NA | NA | 1.0000 | NA | NA | NA | 1.0000 |
| dihydrouridine | NA | NA | 1.3031 | NA | NA | NA | 0.6969 |
| guanosine | NA | NA | 1.0000 | NA | NA | NA | 1.0000 |
| inosine | NA | NA | 0.9065 | NA | NA | NA | 1.0935 |
| isowyosine | NA | NA | 1.2715 | NA | NA | NA | 0.7285 |
| N2,2^-O-dimethylguanosine | NA | NA | 1.0582 | NA | NA | NA | 0.9418 |
| N2,7-dimethylguanosine | NA | NA | 1.0153 | NA | NA | NA | 0.9847 |
| N2-methylguanosine | NA | NA | 1.0036 | NA | NA | NA | 0.9964 |
| N2,N2,7-trimethylguanosine | NA | NA | 1.1801 | NA | NA | NA | 0.8199 |
| N2,N2-dimethylguanosine | NA | NA | 1.0244 | NA | NA | NA | 0.9756 |
| N4,2^-O-dimethylcytidine | NA | NA | 0.8555 | NA | NA | NA | 1.1445 |
| N4-acetyl-2^-O-methylcytidine_1 | NA | NA | 0.9518 | NA | NA | NA | 1.0482 |
| N4-acetyl-2^-O-methylcytidine_2 | NA | NA | 1.1476 | NA | NA | NA | 0.8524 |
| N4-acetylcytidine_1 | NA | NA | 0.9625 | NA | NA | NA | 1.0375 |
| N4-acetylcytidine_2 | NA | NA | 0.9733 | NA | NA | NA | 1.0267 |
| N4-methylcytidine_1 | NA | NA | 0.9926 | NA | NA | NA | 1.0074 |
| N4-methylcytidine_2 | NA | NA | 0.9959 | NA | NA | NA | 1.0041 |
| N4,N4,2^-O-trimethylcytidine | NA | NA | 1.0103 | NA | NA | NA | 0.9897 |
| N4,N4-dimethylcytidine | NA | NA | 0.9817 | NA | NA | NA | 1.0183 |
| N6,2^-O-dimethyladenosine | NA | NA | 1.0719 | NA | NA | NA | 0.9281 |
| N6-acetyladenosine_1 | NA | NA | 0.2952 | NA | NA | NA | 1.7048 |
| N6-acetyladenosine_2 | NA | NA | 1.4859 | NA | NA | NA | 0.5141 |
| N6-(cis-hydroxyisopentenyl)adenosine | NA | NA | 1.0486 | NA | NA | NA | 0.9514 |
| N6-formyladenosine_1 | NA | NA | 0.5440 | NA | NA | NA | 1.4560 |
| N6-formyladenosine_2 | NA | NA | 1.4030 | NA | NA | NA | 0.5970 |
| N6-hydroxymethyladenosine_1 | NA | NA | 0.8519 | NA | NA | NA | 1.1481 |
| N6-hydroxymethyladenosine_2 | NA | NA | 0.8699 | NA | NA | NA | 1.1301 |
| N6-isopentenyladenosine_1 | NA | NA | 1.0703 | NA | NA | NA | 0.9297 |
| N6-isopentenyladenosine_2 | NA | NA | 1.1667 | NA | NA | NA | 0.8333 |
| N6-methyladenosine | NA | NA | 0.7084 | NA | NA | NA | 1.2916 |
| N6-methyl-N6-threonylcarbamoyladenosine_1 | NA | NA | 0.7046 | NA | NA | NA | 1.2954 |
| N6-methyl-N6-threonylcarbamoyladenosine_2 | NA | NA | 1.2464 | NA | NA | NA | 0.8357 |
| N6,N6,2^-O-trimethyladenosine | NA | NA | 0.8671 | NA | NA | NA | 1.1994 |
| N6,N6-dimethyladenosine | NA | NA | 1.0356 | NA | NA | NA | 0.9644 |
| N6-threonylcarbamoyladenosine_1 | NA | NA | 1.0905 | NA | NA | NA | 0.9095 |
| N6-threonylcarbamoyladenosine_2 | NA | NA | 0.9247 | NA | NA | NA | 1.0753 |
| O6-methylguanine | NA | NA | 1.5862 | NA | NA | NA | 0.4138 |
| pseudouridine | NA | NA | 1.0033 | NA | NA | NA | 0.9967 |
| uridine | NA | NA | 1.0000 | NA | NA | NA | 1.0000 |
| uridine 5-oxyacetic acid methyl ester_1 | NA | NA | 0.8381 | NA | NA | NA | 1.1619 |
| uridine 5-oxyacetic acid methyl ester_2 | NA | NA | 0.6295 | NA | NA | NA | 1.3705 |
| uridine 5-oxyacetic acid methyl ester_3 | NA | NA | 0.8369 | NA | NA | NA | 1.1631 |
Factors:
| F1 | Genotype:Synthetic 23S_rRNA | Sample source:In vitro transcription |
| F2 | Genotype:TK0008 deletion | Sample source:23S_rRNA_Enrichment |
| F3 | Genotype:TK0008 deletion | Sample source:Total RNA |
| F4 | Genotype:Wild-type | Sample source:23S_Fragment_Depletion |
| F5 | Genotype:Wild-type | Sample source:23S_Fragment_Enrichment |
| F6 | Genotype:Wild-type | Sample source:23S_rRNA_Enrichment |
| F7 | Genotype:Wild-type | Sample source:Total RNA |
