Data for Pilot Study 13C flux effects when RhoC or RhoA perturbed (13C BCs) (Study ST000282)
(Analysis AN000450)Values for each metabolite have been scaled by dividing by the mean across all factors Run Hierarchial cluster analysis on this study | Run Heatmap cluster analysis on this study| Metabolite | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 | F11 | F12 | F13 | F14 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Alanine_123 | 2.3443 | 1.4305 | 0.5135 | 0.1784 | 0.8528 | 0.5188 | 0.9390 | 1.5661 | 1.1471 | 2.7462 | 0.2702 | 0.2577 | 0.7536 | 0.3956 |
| Alanine_123_13C_1 | 1.0474 | NA | 0.4344 | 0.7452 | NA | NA | 1.2602 | 1.6401 | NA | 1.5057 | NA | NA | 0.3064 | 1.0606 |
| Alanine_123_13C_2 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
| Alanine_123_13C_3 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
| alpha-Ketoglutarate_12345 | 0.2224 | 0.1633 | 0.1002 | NA | 10.5944 | 0.0834 | 0.1059 | 0.7327 | 0.0285 | 0.1324 | 0.4850 | 0.2625 | NA | 0.9549 |
| alpha-Ketoglutarate_12345_13C_1 | 0.4643 | 0.4175 | NA | NA | 2.9672 | NA | NA | 1.6755 | NA | NA | 0.0821 | 0.3934 | NA | NA |
| alpha-Ketoglutarate_12345_13C_2 | NA | 0.1840 | NA | NA | NA | NA | NA | 2.7770 | NA | 0.0765 | 1.1731 | 1.1374 | NA | 0.6520 |
| alpha-Ketoglutarate_12345_13C_3 | 3.6967 | NA | 0.0914 | NA | 0.0922 | 0.0761 | NA | 3.2109 | 0.0260 | 1.4025 | 0.3091 | NA | NA | 1.0691 |
| alpha-Ketoglutarate_12345_13C_4 | NA | NA | 0.0181 | NA | NA | 0.0151 | NA | 4.9564 | 0.0052 | NA | NA | NA | NA | NA |
| alpha-Ketoglutarate_12345_13C_5 | 1.9964 | 1.0345 | 0.8339 | NA | 1.2444 | 0.9591 | NA | 0.2373 | 0.4284 | 1.7751 | NA | NA | NA | 1.0627 |
| Aspartate_1234 | 1.8488 | 1.5846 | 0.6228 | 0.2677 | 3.5287 | 0.4826 | 2.0463 | 1.5353 | 0.6934 | 0.2256 | 0.0374 | 0.0299 | 0.0558 | 0.3013 |
| Aspartate_1234_13C_1 | 2.7081 | 2.5424 | 0.7750 | NA | 0.1049 | 0.4782 | 0.3585 | 2.2007 | 0.6347 | 0.2041 | NA | NA | NA | NA |
| Aspartate_1234_13C_2 | 3.1541 | 2.2196 | 0.6485 | 0.2231 | 0.4363 | 0.2689 | 0.5884 | 2.8649 | 1.3833 | 0.1141 | NA | NA | 0.0742 | 0.0528 |
| Aspartate_1234_13C_3 | 1.3038 | 1.4551 | 0.2714 | 0.0947 | 0.6109 | 0.2909 | 0.9378 | 2.7061 | 2.5726 | 0.0823 | NA | NA | 0.0347 | 0.1295 |
| Aspartate_1234_13C_4 | 2.7473 | 2.0997 | 0.6222 | 0.1673 | 1.2867 | 0.5731 | 0.0133 | 0.1732 | 2.5292 | 0.1183 | NA | NA | 0.1172 | 0.0232 |
| Fumarate_1234 | 0.1873 | 0.1702 | 0.1516 | 0.1722 | 8.7512 | 0.1523 | 0.3600 | 0.5337 | 0.2181 | 0.2694 | 0.6809 | 0.3460 | 0.5896 | 2.8395 |
| Fumarate_1234_13C_1 | 0.3409 | 0.2821 | 0.1174 | NA | 3.4107 | NA | 0.4048 | 0.8493 | 0.7311 | NA | 0.1934 | NA | 1.5942 | 2.6714 |
| Fumarate_1234_13C_2 | 0.1429 | NA | NA | NA | 0.0269 | NA | 0.4334 | 1.6139 | 0.6143 | 0.7143 | 1.5846 | 1.2605 | 1.9930 | 1.6161 |
| Fumarate_1234_13C_3 | NA | 0.0096 | NA | NA | 0.3614 | NA | NA | 1.6991 | 1.1472 | 0.4116 | 1.2381 | 0.7760 | 1.8138 | 1.5433 |
| Fumarate_1234_13C_4 | 0.1857 | 0.1686 | NA | NA | 1.2313 | NA | NA | 0.0486 | 1.5304 | 0.4930 | NA | NA | 2.7569 | 1.5854 |
| Glutamate_12345 | 1.4728 | 1.0786 | 0.6464 | 0.3075 | 1.6859 | 0.4118 | 2.3232 | 3.3385 | 0.5156 | 0.7979 | 0.1404 | 0.1222 | 0.1151 | 0.2053 |
| Glutamate_12345_13C_1 | 2.7791 | 2.4596 | 0.6215 | 0.2570 | 0.3669 | 0.6296 | 0.1438 | 2.8082 | 0.5568 | 2.4746 | 0.0248 | NA | NA | 0.1775 |
| Glutamate_12345_13C_2 | 1.4170 | 0.8823 | 0.3115 | 0.1624 | 0.1553 | 0.0795 | 1.0594 | 8.7382 | 0.3504 | 0.9541 | 0.1230 | 0.2069 | 0.0782 | 0.0721 |
| Glutamate_12345_13C_3 | 3.2949 | 2.1744 | 0.9641 | 0.3843 | 1.0807 | 0.6806 | 0.0639 | 1.6653 | 1.3288 | 1.6705 | NA | 0.0260 | 0.1692 | 0.1049 |
| Glutamate_12345_13C_4 | 1.5473 | 0.6829 | 0.7001 | 0.2758 | 1.3732 | 0.6171 | 0.1375 | 5.1925 | 1.4146 | 1.3605 | 0.0454 | 0.0546 | 0.1269 | 0.0571 |
| Glutamate_12345_13C_5 | 2.4883 | 1.4737 | 0.9590 | 0.3413 | 2.1805 | 1.0783 | 0.0064 | 0.1311 | 2.3532 | 2.3159 | 0.0030 | 0.0034 | 0.1954 | 0.1110 |
| Glutamine_12345 | 0.7641 | 0.3725 | 0.1843 | 0.0817 | 1.6388 | 0.2014 | 4.0204 | 1.4492 | 0.2776 | 0.1177 | 1.2614 | 1.0034 | 0.1615 | 0.1682 |
| Glutamine_12345_13C_1 | NA | 0.5905 | NA | NA | 2.5068 | 0.0712 | 1.3926 | 3.0967 | 0.5235 | 0.1238 | NA | 0.1713 | NA | NA |
| Glutamine_12345_13C_2 | NA | 0.2404 | 0.6426 | NA | NA | NA | 2.3473 | 0.7162 | NA | NA | NA | 1.0535 | NA | NA |
| Glutamine_12345_13C_3 | NA | 0.9685 | 0.0016 | 0.0248 | NA | 2.2720 | 1.1374 | NA | 1.6312 | 1.2557 | NA | NA | 0.0373 | 0.9029 |
| Glutamine_12345_13C_4 | 4.1956 | 1.5938 | NA | 0.1371 | NA | 0.3301 | 0.8492 | NA | 0.3862 | 0.1856 | NA | NA | 0.3224 | NA |
| Glutamine_12345_13C_5 | 2.3155 | 1.1892 | 0.7518 | 0.2010 | 1.9095 | 0.8230 | NA | NA | 1.1413 | 0.4190 | NA | NA | 0.6613 | 0.4472 |
| Lactate_123 | 1.2227 | 0.5477 | 0.6000 | 0.2362 | 1.0468 | 0.4815 | 0.5720 | 1.1541 | 0.9635 | 5.6668 | 0.3066 | 0.2649 | 0.8436 | 0.5585 |
| Lactate_123_13C_1 | NA | 0.1819 | NA | NA | NA | NA | 0.4609 | 2.8388 | NA | 3.2493 | 0.0590 | 0.0428 | NA | 0.1672 |
| Lactate_123_13C_2 | 0.5546 | 0.3163 | 0.4280 | 0.3313 | 0.4365 | 0.3573 | 0.9410 | 8.3613 | 0.3367 | 1.0625 | 0.6080 | 0.5078 | 0.3613 | 0.1195 |
| Lactate_123_13C_3 | 0.0150 | 0.0065 | 0.0068 | 0.0011 | 0.0031 | NA | 0.9009 | 12.2004 | 0.0035 | 0.1007 | 0.5176 | 0.3204 | 0.0139 | 0.0055 |
| Oxaloacetate_1234 | 0.4042 | 4.2468 | 0.4195 | 0.3695 | 5.0138 | 0.3059 | 0.3933 | 0.2623 | 0.2506 | 0.1819 | 0.3312 | 0.3254 | 0.2355 | 2.6162 |
| Oxaloacetate_1234_13C_1 | NA | 1.9600 | 0.7200 | 0.0759 | 5.0398 | 0.3475 | 0.4009 | 0.1843 | 0.1856 | NA | NA | 0.0860 | NA | NA |
| Oxaloacetate_1234_13C_2 | 2.5677 | NA | NA | NA | NA | NA | 0.0630 | NA | 0.3693 | NA | NA | NA | NA | NA |
| Oxaloacetate_1234_13C_3 | NA | 0.9559 | NA | 0.3794 | 3.2130 | 1.2317 | NA | NA | NA | 0.2691 | 0.7522 | NA | 0.1987 | NA |
| Oxaloacetate_1234_13C_4 | NA | NA | NA | NA | 1.4277 | NA | NA | NA | NA | NA | NA | NA | NA | 0.5723 |
| Pyruvate_123 | 1.1775 | 0.8706 | 1.4660 | 0.0386 | 3.0100 | 1.3961 | 0.8652 | 0.7266 | 1.5977 | 0.2573 | 0.5239 | 0.6388 | 0.0907 | 0.8781 |
| Pyruvate_123_13C_1 | 1.0292 | 0.4041 | NA | NA | NA | NA | 0.7738 | 4.0011 | NA | NA | 0.4348 | NA | 0.2074 | 0.1497 |
| Pyruvate_123_13C_2 | 0.6426 | 0.1994 | NA | NA | 0.3498 | NA | 1.8674 | 5.5276 | 0.2873 | 0.0052 | 0.9851 | 0.8473 | 0.0594 | 0.0741 |
| Pyruvate_123_13C_3 | 0.0149 | 0.0083 | 0.0096 | NA | NA | 0.0057 | 1.4624 | 7.3564 | 0.0012 | NA | 0.9358 | 0.7386 | NA | 0.0034 |
Factors:
| F1 | Cell line:MCF10A | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F2 | Cell line:MCF10A | 13C Label:Glutamine | RhoC overexpression:yes | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F3 | Cell line:MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F4 | Cell line:MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:yes |
| F5 | Cell line:MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:yes | RhoC drug:no |
| F6 | Cell line:MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:yes | RhoC knockdown:no | RhoC drug:no |
| F7 | Cell line:SUM149 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F8 | Cell line:SUM149 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:yes |
| F9 | Cell line:SUM149 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F10 | Cell line:SUM149 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:yes |
| F11 | Cell line:SUM190 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F12 | Cell line:SUM190 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:yes | RhoC drug:no |
| F13 | Cell line:SUM190 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no |
| F14 | Cell line:SUM190 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:yes | RhoC drug:no |
