Data for 13C mass isotopomer analysis (LCMS flux studies) MLL-AF9 (part I) (Study ST000308)
(Analysis AN000488)Values for each metabolite have been scaled by dividing by the mean across all factors Run Hierarchial cluster analysis on this study | Run Heatmap cluster analysis on this study| Metabolite | F1 | F2 | F3 | F4 | F5 | F6 | F7 |
|---|---|---|---|---|---|---|---|
| 2PG/3PG | 0.7867 | 0.6213 | 1.4722 | 0.7214 | 1.4901 | 0.7667 | 1.0466 |
| 2PG/3PG_13C_1 | NA | NA | NA | NA | NA | NA | NA |
| 2PG/3PG_13C_2 | NA | NA | NA | 0.2904 | 1.6192 | 0.7626 | 1.3279 |
| 2PG/3PG_13C_3 | NA | NA | NA | NA | NA | NA | NA |
| 6PG | 0.7802 | 1.6767 | 1.6472 | 0.9477 | 1.2825 | 0.5215 | 0.8110 |
| 6PG_13C_1 | 0.4262 | 1.0290 | 0.8757 | 0.6838 | 1.1829 | 0.9961 | 1.7842 |
| 6PG_13C_2 | 0.0247 | 0.0261 | 0.0029 | 0.4528 | 1.1632 | 1.4297 | 2.5866 |
| 6PG_13C_3 | 0.2036 | 0.1315 | 0.3399 | 0.4522 | 0.6600 | 1.5471 | 2.8666 |
| 6PG_13C_4 | 0.3242 | 0.3107 | 0.3853 | 0.5005 | 0.7887 | 1.2714 | 2.1582 |
| 6PG_13C_5 | NA | NA | NA | NA | NA | NA | NA |
| 6PG_13C_6 | NA | NA | NA | NA | NA | NA | NA |
| aCoA | 0.7090 | 1.7634 | 1.5902 | 0.5703 | 1.4935 | 0.5970 | 0.9823 |
| aCoA_13C_1 | NA | NA | 1.0000 | NA | NA | NA | NA |
| aCoA_13C_2 | NA | NA | NA | NA | 0.9840 | NA | 1.0320 |
| ADP | 0.7513 | 1.8434 | 1.4201 | 0.9301 | 1.1856 | 0.7094 | 0.8624 |
| ADP_13C_1 | 0.0959 | NA | NA | 0.2923 | 0.3429 | 1.3838 | 2.2823 |
| ADP_13C_2 | 0.0186 | NA | 0.0332 | 0.2505 | 0.5956 | 1.5447 | 2.9129 |
| ADP_13C_3 | 0.0538 | 0.0186 | 0.0687 | 0.4261 | 0.6212 | 1.6425 | 3.2044 |
| ADP_13C_4 | 0.0322 | 0.0089 | 0.0349 | 0.2209 | 0.5521 | 1.6315 | 3.2144 |
| ADP_13C_5 | NA | 0.0949 | 0.0459 | 0.0156 | 0.3315 | 1.1204 | 2.2731 |
| AMP | 0.9848 | 2.0780 | 1.6598 | 0.8811 | 0.8771 | 0.6889 | 0.7690 |
| AMP_13C_1 | NA | NA | NA | 0.2168 | 0.2268 | 1.4108 | 2.1456 |
| AMP_13C_2 | 0.0114 | 0.0218 | 0.0661 | 0.2549 | 0.5408 | 1.7777 | 3.0343 |
| AMP_13C_3 | 0.0461 | 0.0240 | 0.0615 | 0.4393 | 0.4931 | 1.8285 | 3.1439 |
| AMP_13C_4 | 0.0384 | 0.0026 | 0.0413 | 0.2138 | 0.4412 | 1.7748 | 3.1828 |
| AMP_13C_5 | NA | 0.1449 | 0.2254 | 0.2689 | 0.2863 | 1.6262 | 2.3422 |
| ASP | 0.8027 | 1.2527 | 1.3669 | 0.8944 | 0.9953 | 0.9156 | 1.0631 |
| ASP_13C_1 | 0.0602 | 0.0704 | NA | 0.0662 | 0.2336 | 1.6464 | 2.6769 |
| ASP_13C_2 | 0.0084 | 0.0008 | 0.0083 | 0.2602 | 0.6252 | 1.7380 | 2.3708 |
| ASP_13C_3 | NA | 0.1135 | 0.4441 | 0.2428 | 0.3699 | 1.3715 | 1.9919 |
| ASP_13C_4 | NA | 0.0340 | NA | 0.4366 | NA | 0.2738 | 2.2360 |
| ATP | 0.6497 | 0.8945 | 1.2331 | 1.0538 | 1.4125 | 0.8166 | 0.9471 |
| ATP_13C_1 | 0.0754 | NA | 0.1046 | 0.3763 | 0.5164 | 1.6020 | 2.7284 |
| ATP_13C_2 | 0.0385 | NA | 0.0436 | 0.2531 | 0.7043 | 1.6579 | 2.9837 |
| ATP_13C_3 | 0.0512 | NA | 0.0464 | 0.3385 | 0.6631 | 1.3916 | 2.8750 |
| ATP_13C_4 | 0.0358 | NA | 0.0450 | 0.2281 | 0.5456 | 1.4779 | 2.7095 |
| ATP_13C_5 | NA | NA | NA | NA | 0.1300 | 0.6490 | 1.6410 |
| CIT/ICIT | 0.7274 | 1.1649 | 1.6650 | 0.8248 | 1.3519 | 0.6195 | 0.9782 |
| CIT/ICIT_13C_1 | 0.1474 | 0.0891 | 0.2140 | 1.3754 | 0.8697 | 1.9252 | 1.2257 |
| CIT/ICIT_13C_2 | 0.0308 | 0.0247 | 0.0446 | 0.8798 | 1.9054 | 1.3317 | 1.8143 |
| CIT/ICIT_13C_3 | 0.0665 | 0.7542 | 0.2159 | 0.3436 | 0.5912 | 1.6194 | 2.9840 |
| CIT/ICIT_13C_4 | NA | NA | NA | NA | 0.6433 | 0.9011 | 1.4555 |
| CIT/ICIT_13C_5 | NA | 2.2797 | 0.6028 | 0.5389 | 0.7220 | 1.0078 | 1.2622 |
| CIT/ICIT_13C_6 | 0.8439 | 1.4888 | 0.8197 | 0.8562 | 1.0463 | 1.0531 | 1.1578 |
| E4P | 1.2151 | 1.5725 | 1.1142 | 0.8436 | 1.0047 | 0.9863 | 0.6833 |
| E4P_13C_1 | NA | NA | NA | NA | NA | 1.0000 | NA |
| E4P_13C_2 | 0.3333 | NA | 0.3774 | 1.2043 | 1.2073 | 1.6763 | 0.9939 |
| E4P_13C_3 | 0.6016 | 0.5355 | 1.5248 | 0.5649 | 1.3263 | 0.6827 | 1.6295 |
| E4P_13C_4 | NA | NA | 0.4768 | NA | 0.8950 | NA | 1.3666 |
| FBP | 1.5393 | 1.9030 | 1.8268 | 0.7401 | 0.9651 | 0.4907 | 0.4126 |
| FBP_13C_1 | 0.1633 | 0.0816 | 0.1199 | 1.0167 | 1.9229 | 1.5692 | 0.9420 |
| FBP_13C_2 | 0.1758 | 0.0959 | 0.1705 | 0.6269 | 2.7494 | 1.0615 | 1.2407 |
| FBP_13C_3 | 0.0025 | NA | NA | 0.3483 | 1.7725 | 1.3151 | 0.8965 |
| FBP_13C_4 | 0.1576 | NA | 0.1343 | 0.4202 | 2.3205 | 0.9280 | 1.1815 |
| FBP_13C_5 | NA | NA | NA | NA | NA | NA | NA |
| FBP_13C_6 | NA | NA | NA | NA | NA | NA | NA |
| G3P | 1.4403 | 0.8452 | 1.6787 | 1.0106 | 0.8028 | 0.7709 | 0.5745 |
| G3P_13C_1 | NA | NA | NA | 1.0661 | 0.6789 | 1.3879 | 0.8671 |
| G3P_13C_2 | 0.0270 | 0.0168 | 0.0038 | 1.3322 | 1.3341 | 1.3719 | 1.2702 |
| G3P_13C_3 | NA | NA | NA | NA | NA | NA | NA |
| G6P/F6P | 1.8892 | 1.8571 | 1.2683 | 0.8299 | 0.7238 | 0.7003 | 0.3921 |
| G6P/F6P_13C_1 | 0.2415 | 0.2632 | 0.1534 | 1.2970 | 0.9764 | 2.3036 | 0.9915 |
| G6P/F6P_13C_2 | 0.0329 | 0.0310 | 0.0603 | 0.9577 | 1.6580 | 1.9008 | 1.3999 |
| G6P/F6P_13C_3 | NA | NA | NA | 0.6233 | 0.6831 | 1.7361 | 0.9575 |
| G6P/F6P_13C_4 | NA | NA | 0.0945 | 0.5388 | 1.0587 | 1.4535 | 1.2508 |
| G6P/F6P_13C_5 | NA | NA | 0.0036 | 0.4659 | 0.9648 | 2.1910 | 0.5480 |
| G6P/F6P_13C_6 | NA | NA | NA | NA | NA | 1.0000 | NA |
| GI_OH3P | 0.7643 | 1.4728 | 2.4506 | 0.4040 | 1.5171 | 0.3687 | 0.8212 |
| GI_OH3P_13C_1 | 0.0784 | 0.2735 | 0.3253 | 0.7655 | 2.0091 | 1.0535 | 1.4783 |
| GI_OH3P_13C_2 | 0.1514 | NA | 0.2052 | 0.5843 | 2.0486 | 0.8455 | 1.6172 |
| GI_OH3P_13C_3 | 0.0040 | 0.1085 | NA | 0.2122 | 1.8081 | 0.9568 | 1.3751 |
| GLU | 0.6141 | 0.7537 | 1.3025 | 0.9672 | 1.2737 | 0.8201 | 1.2053 |
| GLU_13C_1 | 0.0617 | NA | NA | 0.2998 | 0.2125 | 1.5528 | 2.2979 |
| GLU_13C_2 | 0.0013 | NA | NA | 0.4723 | 0.8579 | 1.3362 | 1.9993 |
| GLU_13C_3 | 0.0004 | 0.0822 | 0.0167 | 0.0974 | 0.2845 | 1.8046 | 2.7804 |
| GLU_13C_4 | 0.0096 | 0.0159 | NA | 0.0495 | 0.3020 | 1.5578 | 2.7490 |
| GLU_13C_5 | NA | 1.8506 | NA | NA | 0.0685 | 0.3256 | 1.2518 |
| LAC | 1.0070 | 1.7615 | 1.5839 | 0.7894 | 1.1104 | 0.6799 | 0.7701 |
| LAC_13C_1 | NA | NA | NA | NA | NA | NA | NA |
| LAC_13C_2 | NA | NA | NA | 0.4935 | 1.4084 | 0.7505 | 1.3476 |
| LAC_13C_3 | NA | NA | NA | NA | NA | NA | NA |
| MAL | 0.8762 | 1.1125 | 1.3440 | 1.0527 | 1.0900 | 0.8006 | 0.9137 |
| MAL_13C_1 | 0.0280 | 0.0208 | 0.0736 | 0.2292 | 0.4809 | 1.9276 | 2.6455 |
| MAL_13C_2 | 0.0398 | 0.0231 | 0.0407 | 0.4655 | 0.8371 | 1.8535 | 2.4494 |
| MAL_13C_3 | 0.2220 | 0.2243 | 0.1758 | 0.3575 | 0.4188 | 2.1460 | 2.6637 |
| MAL_13C_4 | NA | 0.4001 | 0.1267 | 0.4301 | 0.4366 | 1.1416 | 2.1051 |
| mCoA | NA | NA | NA | NA | NA | NA | NA |
| mCoA_13C_1 | NA | NA | NA | NA | NA | NA | NA |
| mCoA_13C_2 | NA | NA | NA | NA | NA | NA | NA |
| mCoA_13C_3 | NA | NA | NA | NA | NA | NA | NA |
| NAD | 0.8263 | 0.4489 | 1.2420 | 1.0280 | 1.2476 | 0.8117 | 1.1087 |
| NAD_13C_1 | 0.0040 | 0.2905 | 0.6262 | 0.2550 | 0.6823 | 0.8280 | 2.6795 |
| NAD_13C_2 | NA | NA | NA | NA | 0.3138 | 0.5389 | 1.7648 |
| NAD_13C_3 | NA | NA | NA | 0.0096 | 0.2049 | 0.2972 | 2.8280 |
| NAD_13C_4 | NA | NA | 0.0450 | 0.0172 | 0.1943 | 0.6450 | 2.5380 |
| NAD_13C_5 | NA | NA | 0.2671 | 0.5298 | 0.0461 | 0.5481 | 1.8696 |
| NADH | 0.8164 | 0.7260 | 0.9210 | 1.1821 | 1.2688 | 1.0064 | 0.8703 |
| NADH_13C_1 | 0.1576 | 0.1759 | 0.1524 | 0.6702 | 0.7881 | 1.5353 | 2.4061 |
| NADH_13C_2 | NA | NA | 0.3066 | 0.1916 | 0.6047 | 1.0489 | 2.3860 |
| NADH_13C_3 | 0.0294 | NA | 0.0485 | NA | 0.6629 | 0.5518 | 2.4260 |
| NADH_13C_4 | NA | NA | 0.0924 | 0.0705 | 0.4225 | 0.9006 | 2.0967 |
| NADH_13C_5 | NA | NA | NA | NA | NA | NA | NA |
| PEP | NA | NA | NA | NA | NA | NA | NA |
| PEP_13C_1 | NA | NA | NA | NA | NA | NA | NA |
| PEP_13C_2 | NA | NA | NA | NA | NA | NA | NA |
| PEP_13C_3 | NA | NA | NA | NA | NA | NA | NA |
| R5P/X5P | 1.3290 | 2.0129 | 1.4755 | 0.7127 | 1.0116 | 0.5950 | 0.6971 |
| R5P/X5P_13C_1 | 0.1126 | 0.0684 | 0.3054 | 0.8597 | 1.3670 | 1.6339 | 1.8004 |
| R5P/X5P_13C_2 | 0.1136 | 0.0499 | 0.1014 | 0.6968 | 1.4120 | 1.5640 | 1.8299 |
| R5P/X5P_13C_3 | NA | NA | 0.0128 | 0.4664 | 1.0806 | 1.1433 | 1.6388 |
| R5P/X5P_13C_4 | 0.0117 | NA | 0.0766 | 0.2572 | 0.7362 | 1.5918 | 2.1338 |
| R5P/X5P_13C_5 | NA | NA | 0.5311 | 0.3824 | NA | 0.9958 | 1.3649 |
| S7P | 1.2511 | 1.5663 | 1.3863 | 0.8848 | 0.9676 | 0.6585 | 0.7917 |
| S7P_13C_1 | 0.1058 | 0.1757 | 0.0868 | 1.2387 | 1.2322 | 1.8291 | 1.1735 |
| S7P_13C_2 | NA | NA | NA | 0.6726 | 0.8290 | 1.3184 | 1.1799 |
| S7P_13C_3 | 0.0295 | NA | 0.0043 | 0.7809 | 1.1194 | 1.8105 | 1.5916 |
| S7P_13C_4 | 0.0315 | 0.1773 | 0.1058 | 0.6478 | 1.1516 | 1.7871 | 1.9296 |
| S7P_13C_5 | 1.0997 | 1.0670 | 1.4104 | 0.8016 | 1.3057 | 0.7120 | 0.7850 |
| S7P_13C_6 | 0.1476 | 0.1355 | 0.2338 | 1.5384 | 2.3544 | 0.5245 | 0.9786 |
| S7P_13C_7 | 0.2005 | NA | NA | 1.0431 | 1.7242 | 0.6696 | 1.0006 |
| SUC | 0.7542 | 1.3955 | 1.4183 | 0.8688 | 1.1308 | 0.7857 | 1.0497 |
| SUC_13C_1 | 0.2852 | 0.1447 | 0.6058 | 0.6383 | 1.2659 | 1.7335 | 1.6250 |
| SUC_13C_2 | NA | NA | 0.6206 | 0.4192 | 0.8456 | 1.5090 | 1.4930 |
| SUC_13C_3 | NA | NA | NA | NA | NA | 0.5792 | 2.2624 |
| SUC_13C_4 | NA | NA | NA | 0.3093 | NA | 1.6907 | NA |
Factors:
| F1 | Timepoint:0 | Type:KO | Treatment:yes |
| F2 | Timepoint:0 | Type:WT | Treatment:no |
| F3 | Timepoint:0 | Type:WT | Treatment:yes |
| F4 | Timepoint:5 | Type:KO | Treatment:yes |
| F5 | Timepoint:5 | Type:WT | Treatment:yes |
| F6 | Timepoint:60 | Type:KO | Treatment:yes |
| F7 | Timepoint:60 | Type:WT | Treatment:yes |
