Data for TCA cycle metabolomics of H3K27M cells grown in regular glutamine media, glutamine free media, and glutamine free media with alpha-ketoglutarate (Part-II) (Study ST001024)
(Analysis AN001681)Values for each metabolite have been scaled by dividing by the mean across all factors Run Hierarchial cluster analysis on this study | Run Heatmap cluster analysis on this study| Metabolite | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 | F11 | F12 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2-Hydroxyglutarate | 0.0583 | 0.1321 | 0.1267 | 0.0558 | 0.0498 | 1.5990 | 0.0707 | 0.0707 | 0.8537 | 0.1918 | 0.1148 | 8.6765 |
| Aspartate | 4.4204 | 0.0889 | 0.3202 | 0.6902 | 0.1067 | 0.0374 | 5.9911 | 0.0409 | 0.2046 | 0.0409 | 0.0267 | 0.0320 |
| cis-Aconitic Acid | 0.0421 | 0.0213 | 0.0446 | 0.0414 | 0.0205 | 0.0517 | 3.0062 | 0.7703 | 1.2460 | 3.1392 | 2.8598 | 0.7569 |
| Citrate | 0.1131 | 0.0738 | 0.1439 | 0.1402 | 0.0689 | 0.1512 | 1.5885 | 1.3033 | 2.1910 | 3.1943 | 2.0201 | 1.0119 |
| Fumarate | 1.3387 | 0.3850 | 0.7758 | 0.8560 | 0.3062 | 0.4754 | 1.6332 | 0.5658 | 1.9147 | 2.0489 | 0.9027 | 0.7977 |
| Glutamate | 1.6845 | 0.1089 | 0.4081 | 1.2900 | 0.0956 | 0.7066 | 2.9280 | 0.0210 | 0.6203 | 0.0642 | 0.0085 | 4.0642 |
| Isocitrate | 0.4426 | 0.4426 | 0.5901 | 0.4426 | 0.4426 | 0.5901 | 1.6819 | 0.4721 | 0.9885 | 2.5376 | 1.7556 | 1.2835 |
| Ketoglutarate | 0.0006 | 0.0003 | 0.0007 | 0.0014 | 0.0003 | 0.1082 | 0.0040 | 0.0043 | 6.4510 | 0.0241 | 0.0083 | 5.3968 |
| Lactate | 0.0217 | 0.0133 | 0.0149 | 0.0838 | 0.0878 | 0.0306 | 2.3220 | 1.9973 | 1.5333 | 2.6556 | 2.7916 | 0.4481 |
| Malate | 2.2068 | 0.1897 | 0.8168 | 0.9079 | 0.1529 | 0.7480 | 1.3761 | 0.3254 | 1.9665 | 1.9386 | 0.6834 | 0.6880 |
| Oxaloacetate | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
| Succinate | 0.1596 | 0.0328 | 0.0670 | 0.1263 | 0.0919 | 0.4712 | 0.1636 | 0.0807 | 3.8605 | 0.9259 | 0.3460 | 5.6745 |
Factors:
| F1 | Sample type:Cells | Grouping:IV Control |
| F2 | Sample type:Cells | Grouping:IV Treatment 1 |
| F3 | Sample type:Cells | Grouping:IV Treatment 2 |
| F4 | Sample type:Cells | Grouping:XVII Control |
| F5 | Sample type:Cells | Grouping:XVII Treatment 1 |
| F6 | Sample type:Cells | Grouping:XVII Treatment 2 |
| F7 | Sample type:Media | Grouping:IV Control |
| F8 | Sample type:Media | Grouping:IV Treatment 1 |
| F9 | Sample type:Media | Grouping:IV Treatment 2 |
| F10 | Sample type:Media | Grouping:XVII Control |
| F11 | Sample type:Media | Grouping:XVII Treatment 1 |
| F12 | Sample type:Media | Grouping:XVII Treatment 2 |
