Data for Effect of cell harvesting technique and storage on metabolic profiles in human skin fibroblasts (Study ST001148)
(Analysis AN001895)Values for each metabolite have been scaled by dividing by the mean across all factors Run Hierarchial cluster analysis on this study | Run Heatmap cluster analysis on this study| Metabolite | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 | F11 | F12 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 3_Methylhistidine | 1.1686 | 1.0449 | 1.2849 | 1.1203 | 1.2396 | 1.4762 | 0.3944 | 0.7669 | 0.2457 | 0.9115 | 0.9922 | 0.9975 |
| 5_Hydroxylysine | 1.0329 | 0.7354 | 0.9415 | 1.0004 | 0.7520 | 0.9342 | 1.0174 | 0.9829 | 0.7930 | 1.2774 | 1.2384 | 1.2944 |
| Alanine | 1.4648 | 1.4421 | 1.3157 | 1.3430 | 1.5146 | 1.2699 | 0.4919 | 0.2034 | 0.3128 | 0.9635 | 0.7880 | 0.8904 |
| alpha_Aminobutyric_acid | 1.5308 | 1.1785 | 1.0735 | 1.5181 | 1.1328 | 0.9922 | 0.3593 | 0.7267 | 0.4509 | 1.2269 | 0.8954 | 0.9149 |
| Aminoadipic_acid | 1.2618 | 0.9270 | 1.1144 | 1.2049 | 1.0173 | 1.1098 | 0.3178 | 0.4392 | 0.3371 | 1.6673 | 1.2939 | 1.3096 |
| Arginine | 1.2686 | 1.3533 | 1.1597 | 1.0535 | 1.3977 | 1.1069 | 1.1770 | 0.3345 | 0.8176 | 0.8649 | 0.6496 | 0.8166 |
| Asparagine | 1.2351 | 1.3167 | 1.1922 | 1.2305 | 1.3220 | 1.1685 | 0.3406 | 0.1846 | 0.3444 | 1.3995 | 1.0668 | 1.1990 |
| Aspartic_Acid | 0.9518 | 0.9115 | 0.8949 | 0.8856 | 1.0366 | 0.8879 | 0.2275 | 0.1907 | 0.2383 | 1.9410 | 1.8613 | 1.9730 |
| beta_Alanine | 1.0478 | 0.9804 | 0.8042 | 0.9990 | 1.0034 | 0.7616 | NA | NA | NA | 1.2885 | 1.1390 | 0.9762 |
| beta_Aminoisobutyric_acid | 1.7611 | 1.6492 | 0.7697 | 1.2531 | 1.4987 | 0.5761 | NA | NA | NA | 0.1763 | 0.5486 | NA |
| Carnosine | 0.9910 | 1.2155 | 0.8224 | 0.9663 | 1.2425 | 0.7610 | 0.7941 | 1.9982 | 0.4471 | 0.9134 | 1.0799 | 0.7000 |
| Citrulline | 1.3738 | 1.5185 | 1.4553 | 1.3427 | 1.7289 | 1.3938 | 0.0446 | 0.2355 | 0.0206 | 0.9820 | 0.9430 | 0.9612 |
| Cystathionine | 0.8460 | 1.0036 | 0.8287 | 0.7878 | 0.9355 | 0.8349 | 0.3043 | NA | 0.2120 | 1.7913 | 1.7524 | 1.7036 |
| Cysteine | 1.4846 | 1.2287 | 1.2256 | 1.0773 | 1.2019 | 1.1576 | 0.9138 | NA | 0.7436 | 0.3732 | 0.0785 | 0.6919 |
| Ethanolamine | 0.6018 | 0.7262 | 0.6372 | 0.8515 | 0.8496 | 0.7034 | 0.7442 | 1.2754 | 0.8971 | 1.8012 | 1.5042 | 1.4084 |
| gamma_Aminobutyric_acid | 1.1370 | 0.8986 | 1.0248 | 1.1014 | 1.0406 | 1.0060 | NA | 0.3531 | 0.2804 | 1.6099 | 1.2328 | 1.3153 |
| Glutamic_Acid | 1.0567 | 1.1834 | 1.1510 | 1.0482 | 1.3046 | 1.1332 | 0.2046 | 0.1309 | 0.1683 | 1.5524 | 1.5983 | 1.4684 |
| Glutamine | 1.4074 | 1.1806 | 1.4121 | 1.2982 | 1.3021 | 1.3455 | NA | 0.0406 | 0.0900 | 1.0807 | 0.8191 | 1.0236 |
| Glycine | 1.2843 | 1.1651 | 1.2180 | 1.2271 | 1.3257 | 1.2089 | 0.3453 | 0.1545 | 0.2639 | 1.3485 | 1.2284 | 1.2303 |
| Histidine | 1.1986 | 1.4786 | 1.2832 | 1.2363 | 1.6970 | 1.3027 | 0.3744 | 0.2019 | 0.2626 | 0.9885 | 0.9847 | 0.9916 |
| Hydroxyproline | 1.1996 | 1.0101 | 1.1430 | 1.2020 | 1.1910 | 1.1719 | NA | 0.0635 | 0.0121 | 1.2523 | 0.9798 | 1.1162 |
| Isoleucine | 1.3333 | 1.3151 | 1.1988 | 1.2129 | 1.4139 | 1.1583 | 0.4067 | 0.1999 | 0.2978 | 1.3063 | 1.0645 | 1.0923 |
| Leucine | 1.1676 | 1.1977 | 1.0530 | 1.0698 | 1.2314 | 1.0279 | 0.9548 | 0.3571 | 0.5879 | 1.2777 | 1.0001 | 1.0751 |
| Lysine | 1.3255 | 1.2856 | 1.2200 | 1.1557 | 1.3098 | 1.1575 | 0.9836 | 0.4635 | 0.7365 | 0.9385 | 0.6166 | 0.8072 |
| Methionine | 1.1558 | 1.2065 | 1.1315 | 1.0639 | 1.2850 | 1.1327 | 0.9967 | 0.2885 | 0.7006 | 1.1546 | 0.7996 | 1.0846 |
| Ornithine | 1.5313 | 1.5448 | 1.3732 | 1.4263 | 1.5189 | 1.2654 | 0.2549 | 0.2248 | 0.1929 | 1.0823 | 0.7815 | 0.8037 |
| Phenylalanine | 1.3361 | 1.2406 | 1.2232 | 1.2101 | 1.2973 | 1.2187 | 0.6361 | 0.2685 | 0.4635 | 1.1582 | 0.8945 | 1.0531 |
| Phosphoethanolamine | 0.8590 | 0.5016 | 0.5437 | 0.8904 | 0.4410 | 0.6003 | 0.4944 | 0.4022 | 0.5109 | 2.5850 | 2.2889 | 1.8825 |
| Proline | 1.1775 | 1.1590 | 1.1082 | 1.2003 | 1.3254 | 1.1221 | 0.3000 | 0.1374 | 0.2181 | 1.5084 | 1.4185 | 1.3251 |
| Sarcosine | 1.2226 | 1.1725 | 1.0903 | 1.2059 | 1.3959 | 1.1543 | 0.2578 | NA | 0.0133 | 1.3156 | 1.1190 | 1.0527 |
| Serine | 0.8087 | 0.8430 | 0.7550 | 0.7742 | 0.8932 | 0.7209 | 2.3315 | 0.2901 | 1.2616 | 1.2933 | 0.8390 | 1.1895 |
| Taurine | 1.2912 | 1.2313 | 1.4856 | 1.2561 | 1.4504 | 1.5012 | 0.1311 | 0.0389 | 0.0395 | 1.1639 | 1.2056 | 1.2051 |
| Threonine | 1.2983 | 1.2597 | 1.2498 | 1.2122 | 1.3478 | 1.2038 | 0.5286 | 0.2563 | 0.3934 | 1.1973 | 0.9579 | 1.0949 |
| Tryptophan | 1.3893 | 1.3748 | 1.3235 | 1.2680 | 1.4614 | 1.3051 | 0.2755 | 0.0466 | 0.2266 | 1.1786 | 1.0276 | 1.1230 |
| Tyrosine | 1.2368 | 1.2424 | 1.1249 | 1.1071 | 1.3268 | 1.1333 | 0.8356 | 0.2347 | 0.5540 | 1.1690 | 0.9458 | 1.0896 |
| Valine | 1.2806 | 1.2578 | 1.1502 | 1.2085 | 1.3052 | 1.0915 | 0.7972 | 0.2742 | 0.5002 | 1.1931 | 0.8822 | 1.0593 |
Factors:
| F1 | Harvest_technique:Methanol_dry | Storage_time:2_weeks |
| F2 | Harvest_technique:Methanol_dry | Storage_time:48_hours |
| F3 | Harvest_technique:Methanol_dry | Storage_time:4_weeks |
| F4 | Harvest_technique:Methanol | Storage_time:2_weeks |
| F5 | Harvest_technique:Methanol | Storage_time:48_hours |
| F6 | Harvest_technique:Methanol | Storage_time:4_weeks |
| F7 | Harvest_technique:Scraping | Storage_time:2_weeks |
| F8 | Harvest_technique:Scraping | Storage_time:48_hours |
| F9 | Harvest_technique:Scraping | Storage_time:4_weeks |
| F10 | Harvest_technique:Trypsin | Storage_time:2_weeks |
| F11 | Harvest_technique:Trypsin | Storage_time:48_hours |
| F12 | Harvest_technique:Trypsin | Storage_time:4_weeks |
