{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000097","ANALYSIS_ID":"AN000156","VERSION":"1","CREATED_ON":"10-02-2015"},

"PROJECT":{"PROJECT_TITLE":"13C Metabolomics: Applications at Natural Abundance","INSTITUTE":"University of Florida","DEPARTMENT":"Department of Biochemistry and Molecular Biology","LABORATORY":"Edison","LAST_NAME":"Edison","FIRST_NAME":"Arthur","ADDRESS":"R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245","EMAIL":"aedison@ufl.edu","PHONE":"352-392-4535","PROJECT_SUMMARY":"-"},

"STUDY":{"STUDY_TITLE":"NMR analysis of D. melanogaster","STUDY_TYPE":"Cold vs Hardy","STUDY_SUMMARY":"Twenty replicates of each genetic line (cold hardy and cold tolerant) were collected with 40 flies per replicate.","INSTITUTE":"University of Florida","DEPARTMENT":"Department of Biochemistry and Molecular Biology","LABORATORY":"Arthur Edison","LAST_NAME":"Chaevien","FIRST_NAME":"Clendinen","ADDRESS":"R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245","EMAIL":"csclendinen@ufl.edu","NUM_GROUPS":"2","TOTAL_SUBJECTS":"40","PHONE":"-"},

"SUBJECT":{"SUBJECT_TYPE":"Animals","SUBJECT_SPECIES":"Drosophila melanogaster","TAXONOMY_ID":"7227","GENDER":"Female","SPECIES_GROUP":"Insect"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"H2_1",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_10",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_11",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_12",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_13",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_14",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_15",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_16",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_17",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_18",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_19",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_2",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_20",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_3",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_4",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_5",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_6",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_7",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_8",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"H2_9",
"Factors":{"Cold Susceptibility":"Cold Hardy"}
},
{
"Subject ID":"-",
"Sample ID":"L2_1",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_10",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_11",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_12",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_13",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_14",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_15",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_16",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_17",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_18",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_19",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_2",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_20",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_3",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_4",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_5",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_6",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_7",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_8",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
},
{
"Subject ID":"-",
"Sample ID":"L2_9",
"Factors":{"Cold Susceptibility":"Cold Susceptible"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Polar-phase metabolites were extracted from homogenized whole fly tissue using an aqueous-optimized metabolite extraction protocol. (Wu, H.; Southam, A. D.; Hines, A.; Viant, M. R. Analytical biochemistry 2008.)","SAMPLE_TYPE":"Tissue extract"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Twenty replicates of each genetic line (cold hardy and cold tolerant) were collected with 40 flies per replicate. Polar phase extracted metabolites were dried and reconstituted in 40 ?L of 99% D2O  and pipetted into 1.5-mm NMR tubes.","PROCESSING_METHOD":"Lyophilization","PROCESSING_STORAGE_CONDITIONS":"-80 degree celsius","EXTRACTION_METHOD":"Polar phase extraction"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"-","INSTRUMENT_NAME":"-","COLUMN_NAME":"-"},

"ANALYSIS":{"LABORATORY_NAME":"Edison","ANALYSIS_TYPE":"NMR","SOFTWARE_VERSION":"VNMRJ","OPERATOR_NAME":"Chaevien Clendinen","DATA_FORMAT":"Varian"},

"NM":{"INSTRUMENT_TYPE":"Varian","NMR_EXPERIMENT_TYPE":"1D 1H","SPECTROMETER_FREQUENCY":"600 MHz","NMR_PROBE":"1.5 mm HTS cryoprobe","NMR_SOLVENT":"D2O","NMR_TUBE_SIZE":"1.5 mm","SHIMMING_METHOD":"Manual","PULSE_SEQUENCE":"1D 1H","WATER_SUPPRESSION":"Presaturation","PULSE_WIDTH":"45","RECEIVER_GAIN":"28","OFFSET_FREQUENCY":"4.77","CHEMICAL_SHIFT_REF_CPD":"TSP","TEMPERATURE":"22 degree Celsius","NUMBER_OF_SCANS":"64","DUMMY_SCANS":"16","ACQUISITION_TIME":"2.3s","RELAXATION_DELAY":"2s","SPECTRAL_WIDTH":"12 ppm","NUM_DATA_POINTS_ACQUIRED":"32768","REAL_DATA_POINTS":"16384","ZERO_FILLING":"2x","APODIZATION":"Cosine squared","INSTRUMENT_NAME":"Varian"},

"Data":[{"Bin range(ppm)":"NMR_BINNED_DATA_END","H2_1":"","H2_10":"","H2_11":"","H2_12":"","H2_13":"","H2_14":"","H2_15":"","H2_16":"","H2_17":"","H2_18":"","H2_19":"","H2_2":"","H2_20":"","H2_3":"","H2_4":"","H2_5":"","H2_6":"","H2_7":"","H2_8":"","H2_9":"","L2_1":"","L2_10":"","L2_11":"","L2_12":"","L2_13":"","L2_14":"","L2_15":"","L2_16":"","L2_17":"","L2_18":"","L2_19":"","L2_2":"","L2_20":"","L2_3":"","L2_4":"","L2_5":"","L2_6":"","L2_7":"","L2_8":"","L2_9":""},{"Bin range(ppm)":"#END","H2_1":"","H2_10":"","H2_11":"","H2_12":"","H2_13":"","H2_14":"","H2_15":"","H2_16":"","H2_17":"","H2_18":"","H2_19":"","H2_2":"","H2_20":"","H2_3":"","H2_4":"","H2_5":"","H2_6":"","H2_7":"","H2_8":"","H2_9":"","L2_1":"","L2_10":"","L2_11":"","L2_12":"","L2_13":"","L2_14":"","L2_15":"","L2_16":"","L2_17":"","L2_18":"","L2_19":"","L2_2":"","L2_20":"","L2_3":"","L2_4":"","L2_5":"","L2_6":"","L2_7":"","L2_8":"","L2_9":""}]

}