{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000278","ANALYSIS_ID":"AN000444","VERSION":"1","CREATED_ON":"01-15-2019"},

"PROJECT":{"PROJECT_TITLE":"Viral Effect on Metabolism","PROJECT_TYPE":"Glycolysis/TCA/Nucleotide analysis (tissue/cells)","PROJECT_SUMMARY":"Global Metabolic profiling of infection by respiratory virus","INSTITUTE":"University of Texas Medical Branch","DEPARTMENT":"Department of Pediatrics","LABORATORY":"Casola Lab","LAST_NAME":"Casola","FIRST_NAME":"Antonella","ADDRESS":"Galveston, TX","EMAIL":"yazhao1@utmb.edu","PHONE":"409-772-2798","DOI":"http://dx.doi.org/10.21228/M8TP4R"},

"STUDY":{"STUDY_TITLE":"Viral Effect on Metabolism  (part I)","STUDY_TYPE":"Glycolysis/TCA/Nucleotide analysis (tissue/cells)","STUDY_SUMMARY":"Small airway epithelial cell (SAEC) (Lonza Walkersville, Inc., MD) were grown according to the manufacturer's instructions in growth medium (SAGM) containing 0.03 mg/ml bovine pituitary extract (BPE), 0.5 µg/ml hydrocortisone, 0.5 ng/ml hEGF, 0.5 µg/ml epinephrine, 10 µg/ml transferrin, 5 µg/ml insulin, 0.1 ng/ml retinoic acid, 6.5ng/ml triiodothyronine, 50 µg/ml gentamicin and 50ng/ml Amphotericin-B, and 0.5 mg/ml bovine serum albumin (BSA, fatty acid free). When SAE grew to around 80 to 90% confluence, the cells were put into basal medium not supplemented with growth factors 3hours. Then the cell monolayers were infected with naïve hMPV at multiplicity of infection (MOI) of 3 to certain time. The supernatant was saved and cells were harvested and stored exactly according to the protocol provided by University of Michigan Metabolomics Core Facility for metabolomics analysis. 50mM ammonium acetate in water was used for rinse buffer.","INSTITUTE":"University of Michigan","DEPARTMENT":"Department of Pediatrics","LABORATORY":"Casola Lab (University of Texas Medical Branch)","LAST_NAME":"Zhao","FIRST_NAME":"Yanhua","ADDRESS":"Galveston, TX","EMAIL":"yazhao1@utmb.edu","PHONE":"409-443-1461"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","SPECIES_GROUP":"Human"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"S00016664",
"Factors":{"INFECTION TIME (0-24 Hrs)":"0"}
},
{
"Subject ID":"-",
"Sample ID":"S00016665",
"Factors":{"INFECTION TIME (0-24 Hrs)":"0"}
},
{
"Subject ID":"-",
"Sample ID":"S00016666",
"Factors":{"INFECTION TIME (0-24 Hrs)":"0"}
},
{
"Subject ID":"-",
"Sample ID":"S00016670",
"Factors":{"INFECTION TIME (0-24 Hrs)":"15"}
},
{
"Subject ID":"-",
"Sample ID":"S00016671",
"Factors":{"INFECTION TIME (0-24 Hrs)":"15"}
},
{
"Subject ID":"-",
"Sample ID":"S00016672",
"Factors":{"INFECTION TIME (0-24 Hrs)":"15"}
},
{
"Subject ID":"-",
"Sample ID":"S00016673",
"Factors":{"INFECTION TIME (0-24 Hrs)":"24"}
},
{
"Subject ID":"-",
"Sample ID":"S00016674",
"Factors":{"INFECTION TIME (0-24 Hrs)":"24"}
},
{
"Subject ID":"-",
"Sample ID":"S00016675",
"Factors":{"INFECTION TIME (0-24 Hrs)":"24"}
},
{
"Subject ID":"-",
"Sample ID":"S00016667",
"Factors":{"INFECTION TIME (0-24 Hrs)":"6"}
},
{
"Subject ID":"-",
"Sample ID":"S00016668",
"Factors":{"INFECTION TIME (0-24 Hrs)":"6"}
},
{
"Subject ID":"-",
"Sample ID":"S00016669",
"Factors":{"INFECTION TIME (0-24 Hrs)":"6"}
}
],
"COLLECTION":{"SAMPLE_TYPE":"Cell Line","COLLECTION_SUMMARY":"-"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_PROTOCOL_FILENAME":"Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx","SAMPLEPREP_SUMMARY":"-"},

"CHROMATOGRAPHY":{"METHODS_ID":"AQM011","METHODS_FILENAME":"ALPHA_KETO_ACIDS-FULL.M.zip","INSTRUMENT_NAME":"Agilent 7890A","COLUMN_NAME":"Agilent DB5-MS (30m × 0.25mm, 0.25um)","CHROMATOGRAPHY_TYPE":"GC"},

"ANALYSIS":{"LABORATORY_NAME":"MRC2 (University of Michigan)","ANALYSIS_TYPE":"MS","ACQUISITION_PARAMETERS_FILE":"ALPHA_KETO_ACIDS-FULL.M","PROCESSING_PARAMETERS_FILE":"EX00368-MassHunterQuant-GlyTCA-GC-DataAnalysis-Method.m"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 5975C","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE"},

"MS_METABOLITE_DATA":{
"Units":"pmol/µg protein",

"Data":[{"Metabolite":"2 or 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or Isocitrate","S00016664":"62.5318","S00016665":"41.2233","S00016666":"46.9292","S00016670":"85.2503","S00016671":"73.2136","S00016672":"57.9834","S00016673":"69.0360","S00016674":"63.8405","S00016675":"63.8592","S00016667":"87.4194","S00016668":"94.3143","S00016669":"82.4112"},{"Metabolite":"Erythrose 4-phosphate","S00016664":"47.7812","S00016665":"11.9974","S00016666":"13.9048","S00016670":"26.1320","S00016671":"22.2451","S00016672":"28.3171","S00016673":"28.2715","S00016674":"19.4888","S00016675":"32.1705","S00016667":"12.9763","S00016668":"21.8987","S00016669":"28.2255"},{"Metabolite":"flavin adenine dinucleotide","S00016664":"1.3376","S00016665":"0.9147","S00016666":"0.9510","S00016670":"1.3242","S00016671":"0.9666","S00016672":"0.7568","S00016673":"0.9203","S00016674":"0.9364","S00016675":"0.9441","S00016667":"1.2015","S00016668":"1.3112","S00016669":"0.8748"},{"Metabolite":"Fructose 1_6-bisphosphate","S00016664":"108.1136","S00016665":"111.4112","S00016666":"118.5792","S00016670":"78.2383","S00016671":"54.1404","S00016672":"18.0183","S00016673":"61.4530","S00016674":"44.5080","S00016675":"31.6988","S00016667":"121.2532","S00016668":"130.3715","S00016669":"42.5975"},{"Metabolite":"Glucose 6-phosphate or fructose-6-phosphate","S00016664":"60.8559","S00016665":"24.1002","S00016666":"23.4547","S00016670":"36.3224","S00016671":"25.6044","S00016672":"47.0266","S00016673":"29.4174","S00016674":"23.3227","S00016675":"48.7322","S00016667":"26.3124","S00016668":"31.5395","S00016669":"46.0598"},{"Metabolite":"Malate","S00016664":"130.8806","S00016665":"87.9238","S00016666":"93.5793","S00016670":"66.7578","S00016671":"55.5441","S00016672":"41.9971","S00016673":"61.5242","S00016674":"54.5337","S00016675":"55.8292","S00016667":"90.7693","S00016668":"104.7536","S00016669":"79.8110"},{"Metabolite":"Nicotinamide Adenine Dinucleotide oxidized","S00016664":"53.0273","S00016665":"38.0305","S00016666":"41.3879","S00016670":"48.6584","S00016671":"41.4074","S00016672":"31.5627","S00016673":"40.0170","S00016674":"36.0201","S00016675":"35.2974","S00016667":"51.8479","S00016668":"57.7828","S00016669":"41.5902"},{"Metabolite":"nicotinamide adenine dinucleotide phosphate oxidized","S00016664":"6.5771","S00016665":"4.0963","S00016666":"2.0516","S00016670":"4.2182","S00016671":"2.5302","S00016672":"1.1994","S00016673":"2.9342","S00016674":"2.7724","S00016675":"1.8145","S00016667":"4.4684","S00016668":"4.7466","S00016669":"2.5694"},{"Metabolite":"nicotinamide adenine dinucleotide phosphate reduced","S00016664":"10.0757","S00016665":"14.1021","S00016666":"23.2088","S00016670":"3.3065","S00016671":"4.4625","S00016672":"5.6311","S00016673":"1.5999","S00016674":"2.6634","S00016675":"4.5383","S00016667":"15.3767","S00016668":"11.0840","S00016669":"8.0404"},{"Metabolite":"Nicotinamide Adenine Dinucleotide 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"Metabolites":[{"Metabolite":"2 or 3-phosphoglycerate_2PG/3PG","pubchem_id":"724","inchi_key":"","kegg_id":"C00597","other_id":"2PG/3PG","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"6-Phosphogluconate","pubchem_id":"91493","inchi_key":"","kegg_id":"C00345","other_id":"6PG","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Acetyl-CoA","pubchem_id":"444493","inchi_key":"","kegg_id":"C00024","other_id":"aCoA","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"ADP","pubchem_id":"6022","inchi_key":"","kegg_id":"C00008","other_id":"ADP","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"AMP","pubchem_id":"6083","inchi_key":"","kegg_id":"C00020","other_id":"AMP","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"ATP","pubchem_id":"5957","inchi_key":"","kegg_id":"C00002","other_id":"ATP","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Citrate or Isocitrate","pubchem_id":"311","inchi_key":"","kegg_id":"C00158","other_id":"CIT/ICIT","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Erythrose 4-phosphate","pubchem_id":"122357","inchi_key":"","kegg_id":"C00279","other_id":"E4P","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"flavin adenine dinucleotide","pubchem_id":"643975","inchi_key":"","kegg_id":"C00016","other_id":"FAD","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Fructose 1,6-bisphosphate","pubchem_id":"172313","inchi_key":"","kegg_id":"C00354","other_id":"FBP","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Glucose 6-phosphate or fructose-6-phosphate","pubchem_id":"5958","inchi_key":"","kegg_id":"C00092","other_id":"G6P/F6P","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Malate","pubchem_id":"222656","inchi_key":"","kegg_id":"C00149","other_id":"MAL","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Nicotinamide Adenine Dinucleotide oxidized","pubchem_id":"10897651","inchi_key":"","kegg_id":"C00003","other_id":"NAD","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"nicotinamide adenine dinucleotide phosphate oxidized","pubchem_id":"5886","inchi_key":"","kegg_id":"C00006","other_id":"NADP","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"nicotinamide adenine dinucleotide phosphate reduced","pubchem_id":"5886","inchi_key":"","kegg_id":"C00006","other_id":"NADPH","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Nicotinamide Adenine Dinucleotide reduced","pubchem_id":"439153","inchi_key":"","kegg_id":"C00004","other_id":"NADH","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Phosphoenolpyruvate","pubchem_id":"1005","inchi_key":"","kegg_id":"C00074","other_id":"PEP","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Ribose-5-phosphate or xylulose 5-phosphate","pubchem_id":"77982","inchi_key":"","kegg_id":"C00117","other_id":"R5P/X5P","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Sedoheptulose 7-phosphate","pubchem_id":"165007","inchi_key":"","kegg_id":"C05382","other_id":"S7P","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Sucrose","pubchem_id":"5988","inchi_key":"","kegg_id":"C00089","other_id":"SUC","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""}]
}

}