{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000372","ANALYSIS_ID":"AN000606","VERSION":"1","CREATED_ON":"April 18, 2016, 7:27 pm"}, "PROJECT":{"PROJECT_TITLE":"Fly Brain Studies","PROJECT_SUMMARY":"Measure change in metabolites according to diet and feeding state","INSTITUTE":"University of Michigan","DEPARTMENT":"MCDB LS&A","LABORATORY":"Dus Lab","LAST_NAME":"Dus","FIRST_NAME":"Monica","ADDRESS":"Ann Arbor, MI","EMAIL":"mdus@umich.edu","PHONE":"347-891-2287"}, "STUDY":{"STUDY_TITLE":"Measure change in metabolites based on diet and feeding status","STUDY_TYPE":"Regular","STUDY_SUMMARY":"Flies were fed a normal diet or high-sugar (30% sucrose) diet for 10 days. Flies of each diet were flash frozen in liquid nitrogen as sated (refed for 3hrs on 400mM D-glucose) or after fasting for 24hrs. Heads and bodies were separated using a sieve-system and placed immediately on dry ice (total time app. 1min). We collected 50 heads per condition (4 conditions total) and 5 biological replicates","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"4","TOTAL_SUBJECTS":"18"}, "SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Drosophila melanogaster","TAXONOMY_ID":"7227"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"SU0012355", "Sample ID":"S00018443", "Factors":{"State":"sated","Diet":"high sugar"} }, { "Subject ID":"SU0012355", "Sample ID":"S00018444", "Factors":{"State":"sated","Diet":"high sugar"} }, { "Subject ID":"SU0012355", "Sample ID":"S00018445", "Factors":{"State":"sated","Diet":"high sugar"} }, { "Subject ID":"SU0012355", "Sample ID":"S00018446", "Factors":{"State":"sated","Diet":"high sugar"} }, { "Subject ID":"SU0012355", "Sample ID":"S00018447", "Factors":{"State":"sated","Diet":"high sugar"} }, { "Subject ID":"SU0012356", "Sample ID":"S00018448", "Factors":{"State":"sated","Diet":"normal"} }, { "Subject ID":"SU0012356", "Sample ID":"S00018449", "Factors":{"State":"sated","Diet":"normal"} }, { "Subject ID":"SU0012356", "Sample ID":"S00018450", "Factors":{"State":"sated","Diet":"normal"} }, { "Subject ID":"SU0012356", "Sample ID":"S00018451", "Factors":{"State":"sated","Diet":"normal"} }, { "Subject ID":"SU0012357", "Sample ID":"S00018452", "Factors":{"State":"starved","Diet":"normal"} }, { "Subject ID":"SU0012357", "Sample ID":"S00018453", "Factors":{"State":"starved","Diet":"normal"} }, { "Subject ID":"SU0012357", "Sample ID":"S00018454", "Factors":{"State":"starved","Diet":"normal"} }, { "Subject ID":"SU0012357", "Sample ID":"S00018455", "Factors":{"State":"starved","Diet":"normal"} }, { "Subject ID":"SU0012357", "Sample ID":"S00018456", "Factors":{"State":"starved","Diet":"normal"} }, { "Subject ID":"SU0012358", "Sample ID":"S00018457", "Factors":{"State":"starved","Diet":"high sugar"} }, { "Subject ID":"SU0012358", "Sample ID":"S00018458", "Factors":{"State":"starved","Diet":"high sugar"} }, { "Subject ID":"SU0012358", "Sample ID":"S00018459", "Factors":{"State":"starved","Diet":"high sugar"} }, { "Subject ID":"SU0012358", "Sample ID":"S00018460", "Factors":{"State":"starved","Diet":"high sugar"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"none","SAMPLE_TYPE":"Tissues"}, "TREATMENT":{"TREATMENT_SUMMARY":"none","TREATMENT_PROTOCOL_FILENAME":"Acyl-CoA -20150305.docx"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"none","SAMPLEPREP_PROTOCOL_FILENAME":"Acyl-CoA -20150305.docx"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"None","COLUMN_NAME":"Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)","METHODS_FILENAME":"QM-005-Xbrdg2mm-CoA-6490.m.zip","METHODS_ID":"DQM0127"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"QM-005-Xbrdg2mm-CoA-6490.m.zip"}, "MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6410 QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE"}, "MS_METABOLITE_DATA":{ "Units":"pmol/µg tissue protein", "Data":[{"Metabolite":"Acetyl-CoA","S00018443":"","S00018444":"","S00018445":"","S00018446":"","S00018447":"","S00018448":"","S00018449":"","S00018450":"","S00018451":"","S00018452":"","S00018453":"","S00018454":"","S00018455":"","S00018456":"","S00018457":"","S00018458":"","S00018459":"","S00018460":""},{"Metabolite":"Palmitoyl-CoA","S00018443":"","S00018444":"","S00018445":"","S00018446":"","S00018447":"","S00018448":"","S00018449":"","S00018450":"","S00018451":"","S00018452":"","S00018453":"","S00018454":"","S00018455":"","S00018456":"","S00018457":"","S00018458":"","S00018459":"","S00018460":""},{"Metabolite":"Palmitoleoyl-CoA","S00018443":"","S00018444":"","S00018445":"","S00018446":"","S00018447":"","S00018448":"","S00018449":"","S00018450":"","S00018451":"","S00018452":"","S00018453":"","S00018454":"","S00018455":"","S00018456":"","S00018457":"","S00018458":"","S00018459":"","S00018460":""},{"Metabolite":"Stearoyl-CoA","S00018443":"","S00018444":"","S00018445":"","S00018446":"","S00018447":"","S00018448":"","S00018449":"","S00018450":"","S00018451":"","S00018452":"","S00018453":"","S00018454":"","S00018455":"","S00018456":"","S00018457":"","S00018458":"","S00018459":"","S00018460":""},{"Metabolite":"Oleoyl-CoA","S00018443":"0.132","S00018444":"0.132","S00018445":"0.199","S00018446":"0.092","S00018447":"0.124","S00018448":"0.091","S00018449":"0.118","S00018450":"0.033","S00018451":"0.177","S00018452":"0.140","S00018453":"0.247","S00018454":"0.126","S00018455":"0.157","S00018456":"0.208","S00018457":"0.043","S00018458":"0.034","S00018459":"0.001","S00018460":"0.041"},{"Metabolite":"Linoleoyl-CoA","S00018443":"0.536","S00018444":"0.255","S00018445":"0.316","S00018446":"0.219","S00018447":"0.240","S00018448":"0.204","S00018449":"0.285","S00018450":"0.219","S00018451":"0.293","S00018452":"0.325","S00018453":"0.304","S00018454":"0.251","S00018455":"0.238","S00018456":"0.318","S00018457":"0.429","S00018458":"0.259","S00018459":"0.397","S00018460":"0.380"},{"Metabolite":"Eicosanoyl-CoA","S00018443":"","S00018444":"","S00018445":"","S00018446":"","S00018447":"","S00018448":"","S00018449":"","S00018450":"","S00018451":"","S00018452":"","S00018453":"","S00018454":"","S00018455":"","S00018456":"","S00018457":"","S00018458":"","S00018459":"","S00018460":""},{"Metabolite":"11Z-eicosenoyl-CoA","S00018443":"","S00018444":"","S00018445":"","S00018446":"","S00018447":"","S00018448":"","S00018449":"","S00018450":"","S00018451":"","S00018452":"","S00018453":"","S00018454":"","S00018455":"","S00018456":"","S00018457":"","S00018458":"","S00018459":"","S00018460":""},{"Metabolite":"8Z,11Z-eicosadienoyl-CoA","S00018443":"","S00018444":"","S00018445":"","S00018446":"","S00018447":"","S00018448":"","S00018449":"","S00018450":"","S00018451":"","S00018452":"","S00018453":"","S00018454":"","S00018455":"","S00018456":"","S00018457":"","S00018458":"","S00018459":"","S00018460":""},{"Metabolite":"Arachidonyl-CoA","S00018443":"","S00018444":"","S00018445":"","S00018446":"","S00018447":"","S00018448":"","S00018449":"","S00018450":"","S00018451":"","S00018452":"","S00018453":"","S00018454":"","S00018455":"","S00018456":"","S00018457":"","S00018458":"","S00018459":"","S00018460":""},{"Metabolite":"Malonyl-CoA","S00018443":"","S00018444":"","S00018445":"","S00018446":"","S00018447":"","S00018448":"","S00018449":"","S00018450":"","S00018451":"","S00018452":"","S00018453":"","S00018454":"","S00018455":"","S00018456":"","S00018457":"","S00018458":"","S00018459":"","S00018460":""}], 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ID":""},{"Metabolite":"Arachidonyl-CoA","retention index":"","quantified m/z":"","PubChem ID":"5497113","KEGG ID":""},{"Metabolite":"Malonyl-CoA","retention index":"","quantified m/z":"","PubChem ID":"644066","KEGG ID":""}] } }