{
"METABOLOMICS WORKBENCH":{"VERSION":"1","CREATED_ON":"November 15, 2016, 2:21 pm"},

"PROJECT":{"PROJECT_TITLE":"Metabolomic analysis of oxytocin effects on social deficits in mice","PROJECT_SUMMARY":"The goal of this study was to determine the effects of the neuropeptide oxytocin (OT) on metabolomic profiles, using a mouse model of autism-like behavior, the BALB/cByJ inbred strain. We have previously reported that subchronic treatment with OT can lead to persistent reversal of social deficits in BALB/cByJ and other models of autism spectrum disorder (ASD). In this study, mice were given a subchronic regimen with either vehicle or OT, and then evaluated for social approach. At the end of the study, brain and blood were collected for metabolomic analysis. In addition, fecal samples were taken at different time points during the treatment and testing regimen. The results from this project could elucidate mechanisms underlying the prosocial effects of oxytocin, and identify new targets for the development of highly specific oxytocin-related drugs.","INSTITUTE":"University of North Carolina","DEPARTMENT":"Department of Psychiatry","LAST_NAME":"Sheryl","FIRST_NAME":"Moy","ADDRESS":"CB# 7146, Chapel Hill, NC 277599","EMAIL":"ssmoy@med.unc.edu","PHONE":"919-966-3082"},

"STUDY":{"STUDY_TITLE":"Metabolomic analysis of oxytocin effects on social deficits in mice","STUDY_SUMMARY":"The goal of this study is to determine the prosocial hormone oxytocin (OT) effects on metabolomic profiles in brain.","INSTITUTE":"RTI International","LAST_NAME":"Sumner","FIRST_NAME":"Susan","ADDRESS":"3040 E. Cornwallis Road","EMAIL":"ssumner@rti.org","PHONE":"919-541-7479"},

"SUBJECT":{"SUBJECT_TYPE":"Mice","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"A1",
"Sample ID":"B_1",
"Factors":{"Treatment":"Oxytocin","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C2",
"Sample ID":"B_10",
"Factors":{"Treatment":"Oxytocin","Brain region":"OlF"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"D6",
"Sample ID":"B_100",
"Factors":{"Treatment":"Veh","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C3",
"Sample ID":"B_11",
"Factors":{"Treatment":"Veh","Brain region":"OlF"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"C4",
"Sample ID":"B_12",
"Factors":{"Treatment":"Oxytocin","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C5",
"Sample ID":"B_13",
"Factors":{"Treatment":"Veh","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C6",
"Sample ID":"B_14",
"Factors":{"Treatment":"Oxytocin","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D1",
"Sample ID":"B_15",
"Factors":{"Treatment":"Oxytocin","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D2",
"Sample ID":"B_16",
"Factors":{"Treatment":"Veh","Brain region":"OlF"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"D4",
"Sample ID":"B_18",
"Factors":{"Treatment":"Veh","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D5",
"Sample ID":"B_19",
"Factors":{"Treatment":"Oxytocin","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"A4",
"Sample ID":"B_2",
"Factors":{"Treatment":"Oxytocin","Brain region":"OlF"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"D6",
"Sample ID":"B_20",
"Factors":{"Treatment":"Veh","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"A1",
"Sample ID":"B_21",
"Factors":{"Treatment":"Oxytocin","Brain region":"Fore"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"A4",
"Sample ID":"B_22",
"Factors":{"Treatment":"Oxytocin","Brain region":"Fore"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"A5",
"Sample ID":"B_23",
"Factors":{"Treatment":"Oxytocin","Brain region":"Fore"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"B1",
"Sample ID":"B_24",
"Factors":{"Treatment":"Veh","Brain region":"Fore"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B2",
"Sample ID":"B_25",
"Factors":{"Treatment":"Oxytocin","Brain region":"Fore"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"B3",
"Sample ID":"B_26",
"Factors":{"Treatment":"Oxytocin","Brain region":"Fore"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B4",
"Sample ID":"B_27",
"Factors":{"Treatment":"Veh","Brain region":"Fore"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B5",
"Sample ID":"B_28",
"Factors":{"Treatment":"Oxytocin","Brain region":"Fore"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"B6",
"Sample ID":"B_29",
"Factors":{"Treatment":"Veh","Brain region":"Fore"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"A5",
"Sample ID":"B_3",
"Factors":{"Treatment":"Oxytocin","Brain region":"OlF"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"C2",
"Sample ID":"B_30",
"Factors":{"Treatment":"Oxytocin","Brain region":"Fore"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C3",
"Sample ID":"B_31",
"Factors":{"Treatment":"Veh","Brain region":"Fore"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C4",
"Sample ID":"B_32",
"Factors":{"Treatment":"Oxytocin","Brain region":"Fore"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"C5",
"Sample ID":"B_33",
"Factors":{"Treatment":"Veh","Brain region":"Fore"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"C6",
"Sample ID":"B_34",
"Factors":{"Treatment":"Oxytocin","Brain region":"Fore"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D1",
"Sample ID":"B_35",
"Factors":{"Treatment":"Oxytocin","Brain region":"Fore"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D2",
"Sample ID":"B_36",
"Factors":{"Treatment":"Veh","Brain region":"Fore"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D3",
"Sample ID":"B_37",
"Factors":{"Treatment":"Oxytocin","Brain region":"Fore"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"D4",
"Sample ID":"B_38",
"Factors":{"Treatment":"Veh","Brain region":"Fore"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D5",
"Sample ID":"B_39",
"Factors":{"Treatment":"Oxytocin","Brain region":"Fore"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B1",
"Sample ID":"B_4",
"Factors":{"Treatment":"Veh","Brain region":"OlF"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"D6",
"Sample ID":"B_40",
"Factors":{"Treatment":"Veh","Brain region":"Fore"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"A1",
"Sample ID":"B_41",
"Factors":{"Treatment":"Oxytocin","Brain region":"Mid"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"A4",
"Sample ID":"B_42",
"Factors":{"Treatment":"Oxytocin","Brain region":"Mid"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"A5",
"Sample ID":"B_43",
"Factors":{"Treatment":"Oxytocin","Brain region":"Mid"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B1",
"Sample ID":"B_44",
"Factors":{"Treatment":"Veh","Brain region":"Mid"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B2",
"Sample ID":"B_45",
"Factors":{"Treatment":"Oxytocin","Brain region":"Mid"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B3",
"Sample ID":"B_46",
"Factors":{"Treatment":"Oxytocin","Brain region":"Mid"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"B4",
"Sample ID":"B_47",
"Factors":{"Treatment":"Veh","Brain region":"Mid"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B5",
"Sample ID":"B_48",
"Factors":{"Treatment":"Oxytocin","Brain region":"Mid"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B6",
"Sample ID":"B_49",
"Factors":{"Treatment":"Veh","Brain region":"Mid"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B2",
"Sample ID":"B_5",
"Factors":{"Treatment":"Oxytocin","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C2",
"Sample ID":"B_50",
"Factors":{"Treatment":"Oxytocin","Brain region":"Mid"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"C3",
"Sample ID":"B_51",
"Factors":{"Treatment":"Veh","Brain region":"Mid"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C4",
"Sample ID":"B_52",
"Factors":{"Treatment":"Oxytocin","Brain region":"Mid"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C5",
"Sample ID":"B_53",
"Factors":{"Treatment":"Veh","Brain region":"Mid"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C6",
"Sample ID":"B_54",
"Factors":{"Treatment":"Oxytocin","Brain region":"Mid"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"D1",
"Sample ID":"B_55",
"Factors":{"Treatment":"Oxytocin","Brain region":"Mid"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"D2",
"Sample ID":"B_56",
"Factors":{"Treatment":"Veh","Brain region":"Mid"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D3",
"Sample ID":"B_57",
"Factors":{"Treatment":"Oxytocin","Brain region":"Mid"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D4",
"Sample ID":"B_58",
"Factors":{"Treatment":"Veh","Brain region":"Mid"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D5",
"Sample ID":"B_59",
"Factors":{"Treatment":"Oxytocin","Brain region":"Mid"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"B3",
"Sample ID":"B_6",
"Factors":{"Treatment":"Oxytocin","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D6",
"Sample ID":"B_60",
"Factors":{"Treatment":"Veh","Brain region":"Mid"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"A1",
"Sample ID":"B_61",
"Factors":{"Treatment":"Oxytocin","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"A4",
"Sample ID":"B_62",
"Factors":{"Treatment":"Oxytocin","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"A5",
"Sample ID":"B_63",
"Factors":{"Treatment":"Oxytocin","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B1",
"Sample ID":"B_64",
"Factors":{"Treatment":"Veh","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B2",
"Sample ID":"B_65",
"Factors":{"Treatment":"Oxytocin","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B3",
"Sample ID":"B_66",
"Factors":{"Treatment":"Oxytocin","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B4",
"Sample ID":"B_67",
"Factors":{"Treatment":"Veh","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B5",
"Sample ID":"B_68",
"Factors":{"Treatment":"Oxytocin","Brain region":"Cerb"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"B6",
"Sample ID":"B_69",
"Factors":{"Treatment":"Veh","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B4",
"Sample ID":"B_7",
"Factors":{"Treatment":"Veh","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C2",
"Sample ID":"B_70",
"Factors":{"Treatment":"Oxytocin","Brain region":"Cerb"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"C3",
"Sample ID":"B_71",
"Factors":{"Treatment":"Veh","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C4",
"Sample ID":"B_72",
"Factors":{"Treatment":"Oxytocin","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C5",
"Sample ID":"B_73",
"Factors":{"Treatment":"Veh","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C6",
"Sample ID":"B_74",
"Factors":{"Treatment":"Oxytocin","Brain region":"Cerb"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"D1",
"Sample ID":"B_75",
"Factors":{"Treatment":"Oxytocin","Brain region":"Cerb"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"D2",
"Sample ID":"B_76",
"Factors":{"Treatment":"Veh","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D3",
"Sample ID":"B_77",
"Factors":{"Treatment":"Oxytocin","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D4",
"Sample ID":"B_78",
"Factors":{"Treatment":"Veh","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D5",
"Sample ID":"B_79",
"Factors":{"Treatment":"Oxytocin","Brain region":"Cerb"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B5",
"Sample ID":"B_8",
"Factors":{"Treatment":"Oxytocin","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D6",
"Sample ID":"B_80",
"Factors":{"Treatment":"Veh","Brain region":"Cerb"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"A1",
"Sample ID":"B_81",
"Factors":{"Treatment":"Oxytocin","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"A4",
"Sample ID":"B_82",
"Factors":{"Treatment":"Oxytocin","Brain region":"Hind"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"A5",
"Sample ID":"B_83",
"Factors":{"Treatment":"Oxytocin","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B1",
"Sample ID":"B_84",
"Factors":{"Treatment":"Veh","Brain region":"Hind"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"B2",
"Sample ID":"B_85",
"Factors":{"Treatment":"Oxytocin","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B3",
"Sample ID":"B_86",
"Factors":{"Treatment":"Oxytocin","Brain region":"Hind"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"B4",
"Sample ID":"B_87",
"Factors":{"Treatment":"Veh","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B5",
"Sample ID":"B_88",
"Factors":{"Treatment":"Oxytocin","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B6",
"Sample ID":"B_89",
"Factors":{"Treatment":"Veh","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"B6",
"Sample ID":"B_9",
"Factors":{"Treatment":"Veh","Brain region":"OlF"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C2",
"Sample ID":"B_90",
"Factors":{"Treatment":"Oxytocin","Brain region":"Hind"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"C3",
"Sample ID":"B_91",
"Factors":{"Treatment":"Veh","Brain region":"Hind"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"C4",
"Sample ID":"B_92",
"Factors":{"Treatment":"Oxytocin","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C5",
"Sample ID":"B_93",
"Factors":{"Treatment":"Veh","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"C6",
"Sample ID":"B_94",
"Factors":{"Treatment":"Oxytocin","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D1",
"Sample ID":"B_95",
"Factors":{"Treatment":"Oxytocin","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D2",
"Sample ID":"B_96",
"Factors":{"Treatment":"Veh","Brain region":"Hind"},
"Additional sample data":{"Pool":"N"}
},
{
"Subject ID":"D3",
"Sample ID":"B_97",
"Factors":{"Treatment":"Oxytocin","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D4",
"Sample ID":"B_98",
"Factors":{"Treatment":"Veh","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"D5",
"Sample ID":"B_99",
"Factors":{"Treatment":"Oxytocin","Brain region":"Hind"},
"Additional sample data":{"Pool":"Y"}
},
{
"Subject ID":"Pool",
"Sample ID":"PB_1",
"Factors":{"Treatment":"Pool","Brain region":"Pool"},
"Additional sample data":{"Pool":"Pool"}
},
{
"Subject ID":"Pool",
"Sample ID":"PB_2",
"Factors":{"Treatment":"Pool","Brain region":"Pool"},
"Additional sample data":{"Pool":"Pool"}
},
{
"Subject ID":"Pool",
"Sample ID":"PB_3",
"Factors":{"Treatment":"Pool","Brain region":"Pool"},
"Additional sample data":{"Pool":"Pool"}
},
{
"Subject ID":"Pool",
"Sample ID":"PB_4",
"Factors":{"Treatment":"Pool","Brain region":"Pool"},
"Additional sample data":{"Pool":"Pool"}
},
{
"Subject ID":"Pool",
"Sample ID":"PB_5",
"Factors":{"Treatment":"Pool","Brain region":"Pool"},
"Additional sample data":{"Pool":"Pool"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"24 hr after the social test, mice were deeply anesthetized with 5% isoflurane, followed by rapid decapitation. Brains were removed, rinsed with ice-cold water, and then rapidly dissected into the following 5 regions: forebrain, midbrain, cerebellum, hindbrain, and olfactory bulbs. The dissected parts were flash-frozen on dry ice and stored at -80o C. Samples were coded by treatment: vehicle-treated mice (n=8) and OT-treated mice (n=12)."},

"TREATMENT":{"TREATMENT_SUMMARY":"BALB/cByJ male mice (4-5 weeks in age; Jackson Laboratory, Bar Harbor, ME) were given four treatments of either vehicle or OT (1.0 mg/kg; IP) across 8 days, with at least 48 hours between each injection. Mice were evaluated in a 3-chamber choice test for social preference 24 hr after the final injection."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Each thawed tissue sample was transferred into a pre-labeled MagnaLyser tube and massed. 50% acetonitrile in water was added depending on the mass of each sample (500 uL for up to 200 mg and 1000 uL for more than 200 mg). Samples were homogenized using MagnaLyser for two 30 sec pulses and then centrifuged. A volume of each sample supernatant required to analyze the desired sample mass (20 mg for Mid, Cerb, Hind, Fore, and 10 mg for Olf) was aliquoted. For a subset of samples with excess material, a total study pool was created by mixing the remaining volume of extracted tissue. Five replicates of the total study pool were created and prepared identically to the study samples. Samples were frozen and lyophilized. Lyophilized samples were mixed with 250 μl of Master mix (0.2 mM phosphate buffer (pH 7.5) containing 10% Chenomx Internal Standard - 0.5 mM DSS-d6 and 6mM Imidazole). The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"-","INSTRUMENT_NAME":"-","COLUMN_NAME":"-"},

"ANALYSIS":{"ANALYSIS_TYPE":"NMR"},

"NM":{"INSTRUMENT_NAME":"Bruker Avance III","INSTRUMENT_TYPE":"FT-NMR","NMR_EXPERIMENT_TYPE":"1D-1H","SPECTROMETER_FREQUENCY":"700.13 MHz"},

"Data":[{"Bin range(ppm)":"[0.70 .. 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