{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000766","ANALYSIS_ID":"AN001212","VERSION":"1","CREATED_ON":"July 5, 2017, 9:57 am"}, "PROJECT":{"PROJECT_TITLE":"Soybean project","PROJECT_SUMMARY":"Non-targeted metabolic profiling of 26 soybean varieties using liquid chromatography-mass spectrometry (LC-MS) including 15 wild black soybeans (WBS) and 11 cultivated black soybeans (CBS), combined with multivariate analysis, revealed significant differences in25 differential metabolites","INSTITUTE":"KIST","LAST_NAME":"Xu","FIRST_NAME":"Jiuliang","ADDRESS":"saimdang-ro 679, Gangneung, Gangwon, 213510, Korea, South","EMAIL":"213510@kist.re.kr","PHONE":"01024398802"}, "STUDY":{"STUDY_TITLE":"Differences in metabolite profiles and antioxidant activities between wild and cultivated black soybeans evaluated by a correlation analysis","STUDY_SUMMARY":"Non-targeted metabolic profiling of 26 soybean varieties using liquid chromatography-mass spectrometry (LC-MS) including 15 wild black soybeans (WBS) and 11 cultivated black soybeans (CBS), combined with multivariate analysis, revealed significant differences in 25 differential metabolites","INSTITUTE":"KIST","LAST_NAME":"Xu","FIRST_NAME":"Jiuliang","ADDRESS":"saimdang-ro 679, Gangneung, Gangwon, 213510, Korea, South","EMAIL":"213510@kist.re.kr","PHONE":"01024398802"}, "SUBJECT":{"SUBJECT_TYPE":"plant","SUBJECT_SPECIES":"Glycine max","TAXONOMY_ID":"3847"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"01_CBS_1", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"02_CBS_2", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"03_CBS_3", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"04_CBS_4", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"05_CBS_5", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"06_CBS_6", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"07_CBS_7", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"11_CBS_8", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"12_CBS_9", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"13_CBS_10", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"14_CBS_11", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"08_CBS_QC1", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"18_CBS_QC2", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"28_CBS_QC3", "Factors":{"type":"Cultivar"} }, { "Subject ID":"-", "Sample ID":"15_WBS_1", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"16_WBS_2", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"17_WBS_3", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"21_WBS_4", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"22_WBS_5", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"23_WBS_6", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"24_WBS_7", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"25_WBS_8", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"26_WBS_9", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"27_WBS_10", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"31_WBS_11", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"32_WBS_12", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"33_WBS_13", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"34_WBS_14", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"35_WBS_15", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"09_WBS_QC1", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"19_WBS_QC2", "Factors":{"type":"Wild"} }, { "Subject ID":"-", "Sample ID":"29_WBS_QC3", "Factors":{"type":"Wild"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Fifteen wild and 11 cultivated black soybeans were selected from the soybean germplasm collections that have been maintained by the National Institute of Crop Science in Korea"}, "TREATMENT":{"TREATMENT_SUMMARY":"The planting density was 15 cm between plants in a row, and the rows were 70 cm apart. Field management, including irrigation, fertilizer application, and pest control followed essentially normal agricultural practices according to Rural Development Administration standard protocol"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Dried black soybeans were ground into a fine powder and sieved through a screen with a 55 mesh count, then 40 mg of the powder was mixed with 70% aqueous methanol (4mL, 1% HCl) containing 1.5 μg/ml phlorizin as an internal standard and sonicated for 30 min. The mixture was kept for approximately 16 h at room temperature in the dark.vacuum.Supernatants were filtered through a 0.20-μm syringe filter and freeze dried then reconstituted with 0.5 ml of 50% aqueous methanol for LC-MS analysis."}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 1290","COLUMN_NAME":"Prevail C18"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Bruker Daltonics","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_RESULTS_FILE":"ST000766_AN001212_Results.txt UNITS:peal area"} }