{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000829","ANALYSIS_ID":"AN001321","VERSION":"1","CREATED_ON":"August 8, 2017, 12:14 pm"}, "PROJECT":{"PROJECT_TITLE":"Mice Retina & Plasma Studies","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"Control and 12W STZ B6 Mice Retina and Plasma","INSTITUTE":"University of Michigan","DEPARTMENT":"Ophthalmology & Visual Sciences","LABORATORY":"Abcouwer Lab","LAST_NAME":"Abcouwer","FIRST_NAME":"Steven","ADDRESS":"Ann Arbor, MI","EMAIL":"sabcouwe@umich.edu","PHONE":"734-232-8227"}, "STUDY":{"STUDY_TITLE":"12 weeks control & STZ B6 mice retina & plasma amino acid analysis","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"Mice were injected with vehicle or STZ to induce diabetes. After 12 weeks, retinas and plasma were harvested.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"4","TOTAL_SUBJECTS":"2"}, "SUBJECT":{"SUBJECT_TYPE":"MOUSE","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"SU0012636", "Sample ID":"S00019142", "Factors":{"Sample type":"Retina","Type":"Control"} }, { "Subject ID":"SU0012636", "Sample ID":"S00019143", "Factors":{"Sample type":"Retina","Type":"Control"} }, { "Subject ID":"SU0012636", "Sample ID":"S00019144", "Factors":{"Sample type":"Retina","Type":"Control"} }, { "Subject ID":"SU0012636", "Sample ID":"S00019145", "Factors":{"Sample type":"Retina","Type":"Control"} }, { "Subject ID":"SU0012636", "Sample ID":"S00019146", "Factors":{"Sample type":"Retina","Type":"Control"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019147", "Factors":{"Sample type":"Retina","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019148", "Factors":{"Sample type":"Retina","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019149", "Factors":{"Sample type":"Retina","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019150", "Factors":{"Sample type":"Retina","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019151", "Factors":{"Sample type":"Retina","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019152", "Factors":{"Sample type":"Retina","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019153", "Factors":{"Sample type":"Retina","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019154", "Factors":{"Sample type":"Retina","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019155", "Factors":{"Sample type":"Retina","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019156", "Factors":{"Sample type":"Retina","Type":"12W STZ"} }, { "Subject ID":"SU0012636", "Sample ID":"S00019157", "Factors":{"Sample type":"Plasma","Type":"Control"} }, { "Subject ID":"SU0012636", "Sample ID":"S00019158", "Factors":{"Sample type":"Plasma","Type":"Control"} }, { "Subject ID":"SU0012636", "Sample ID":"S00019159", "Factors":{"Sample type":"Plasma","Type":"Control"} }, { "Subject ID":"SU0012636", "Sample ID":"S00019160", "Factors":{"Sample type":"Plasma","Type":"Control"} }, { "Subject ID":"SU0012636", "Sample ID":"S00019161", "Factors":{"Sample type":"Plasma","Type":"Control"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019162", "Factors":{"Sample type":"Plasma","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019163", "Factors":{"Sample type":"Plasma","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019164", "Factors":{"Sample type":"Plasma","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019165", "Factors":{"Sample type":"Plasma","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019166", "Factors":{"Sample type":"Plasma","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019167", "Factors":{"Sample type":"Plasma","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019168", "Factors":{"Sample type":"Plasma","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019169", "Factors":{"Sample type":"Plasma","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019170", "Factors":{"Sample type":"Plasma","Type":"12W STZ"} }, { "Subject ID":"SU0012637", "Sample ID":"S00019171", "Factors":{"Sample type":"Plasma","Type":"12W STZ"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"-"}, "TREATMENT":{"TREATMENT_SUMMARY":"-"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"A005_Amino_Acids.pdf"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890N","COLUMN_NAME":"Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm (Phenomenex)"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"A005 - Amino Acids.pdf"}, "MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 5975","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE"}, "MS_METABOLITE_DATA":{ "Units":"uM", "Data":[{"Metabolite":"L-PROLINE","S00019157":"40.61993724","S00019158":"22.32183617","S00019159":"38.01549219","S00019160":"45.09219288","S00019161":"29.3458431","S00019162":"36.09047393","S00019163":"41.31696125","S00019164":"59.07136921","S00019165":"55.48931556","S00019166":"34.80282878","S00019167":"26.64519471","S00019168":"40.91440211","S00019169":"35.34802578","S00019170":"63.36175763","S00019171":"45.18939862"},{"Metabolite":"L-GLUTAMIC ACID","S00019157":"10.78098443","S00019158":"10.91652363","S00019159":"12.17367097","S00019160":"17.46678542","S00019161":"15.60973145","S00019162":"10.99485464","S00019163":"12.50402282","S00019164":"8.89646888","S00019165":"8.557534158","S00019166":"3.224715899","S00019167":"4.6367922","S00019168":"6.424739037","S00019169":"6.765492962","S00019170":"8.113231448","S00019171":"11.39111083"},{"Metabolite":"L-ALANINE","S00019157":"176.8739724","S00019158":"125.2838437","S00019159":"198.6499401","S00019160":"214.3912914","S00019161":"144.5690588","S00019162":"190.8699122","S00019163":"184.8524096","S00019164":"219.1721867","S00019165":"260.764197","S00019166":"121.9565911","S00019167":"98.30946481","S00019168":"148.5426852","S00019169":"161.4965267","S00019170":"178.0700206","S00019171":"151.9569447"},{"Metabolite":"L-SERINE","S00019157":"16.57896624","S00019158":"17.76445556","S00019159":"35.85150512","S00019160":"38.92760397","S00019161":"25.43974376","S00019162":"35.85651662","S00019163":"36.82142061","S00019164":"38.39252703","S00019165":"72.10275983","S00019166":"36.76576803","S00019167":"28.79783438","S00019168":"26.01635513","S00019169":"32.22795007","S00019170":"63.57891583","S00019171":"42.58649444"},{"Metabolite":"L-ASPARTIC ACID","S00019157":"1.308806694","S00019158":"1.351247413","S00019159":"1.844263426","S00019160":"1.927355222","S00019161":"1.633531997","S00019162":"1.784402522","S00019163":"2.378226821","S00019164":"2.513874314","S00019165":"1.354954676","S00019166":".412258662","S00019167":".790464539","S00019168":".842489442","S00019169":".865524286","S00019170":"1.223425993","S00019171":"2.551528446"},{"Metabolite":"L-GLUTAMINE","S00019157":"4.140935914","S00019158":"","S00019159":"","S00019160":"","S00019161":"","S00019162":"8.152663117","S00019163":"","S00019164":"","S00019165":"","S00019166":"","S00019167":"","S00019168":"","S00019169":"8.012927588","S00019170":"","S00019171":""},{"Metabolite":"L-LYSINE","S00019157":"97.06048004","S00019158":"106.783139","S00019159":"150.1656953","S00019160":"110.936258","S00019161":"91.0146733","S00019162":"58.51750124","S00019163":"76.38858654","S00019164":"73.6942849","S00019165":"58.40705572","S00019166":"32.00402203","S00019167":"29.58380482","S00019168":"25.79505481","S00019169":"25.24272214","S00019170":"102.9208797","S00019171":"56.08866517"},{"Metabolite":"L-TYROSINE","S00019157":"37.41526763","S00019158":"28.45427913","S00019159":"30.1172146","S00019160":"31.0181571","S00019161":"28.70958904","S00019162":"20.94388841","S00019163":"27.65814583","S00019164":"41.65054229","S00019165":"35.15764089","S00019166":"14.83627476","S00019167":"29.07380557","S00019168":"21.68845976","S00019169":"16.27334781","S00019170":"80.30021849","S00019171":"33.67936868"},{"Metabolite":"L-LEUCINE","S00019157":"68.48633511","S00019158":"34.06217041","S00019159":"47.51472926","S00019160":"64.19669809","S00019161":"47.01758965","S00019162":"67.93428008","S00019163":"98.98392942","S00019164":"116.8205429","S00019165":"187.79656","S00019166":"51.35256728","S00019167":"67.11385491","S00019168":"84.90549691","S00019169":"67.11549648","S00019170":"216.3285184","S00019171":"156.535194"},{"Metabolite":"2-AMINOISOBUTYRIC ACID","S00019157":"2.963241053","S00019158":"1.333498513","S00019159":"2.09231066","S00019160":"3.221657812","S00019161":"1.411861011","S00019162":"1.374212785","S00019163":"2.449745866","S00019164":"2.203698276","S00019165":"3.051558843","S00019166":"1.066780089","S00019167":"1.310611509","S00019168":"1.332468127","S00019169":"1.555369724","S00019170":"3.012408164","S00019171":"3.4606322"},{"Metabolite":"L-METHIONINE","S00019157":"14.65226498","S00019158":"6.838560192","S00019159":"14.87604432","S00019160":"13.65230568","S00019161":"11.03774101","S00019162":"8.627787716","S00019163":"13.26602534","S00019164":"23.81658834","S00019165":"26.08978836","S00019166":"7.036821227","S00019167":"3.869877439","S00019168":"5.453003294","S00019169":"4.213807478","S00019170":"41.5932986","S00019171":"11.78546398"},{"Metabolite":"L-PHENYLALANINE","S00019157":"27.39407982","S00019158":"22.07575717","S00019159":"24.15479362","S00019160":"28.11132449","S00019161":"25.38800708","S00019162":"25.41012467","S00019163":"29.32567674","S00019164":"29.88082916","S00019165":"33.65543371","S00019166":"18.12130086","S00019167":"19.62072404","S00019168":"20.06591437","S00019169":"21.5201888","S00019170":"40.83707644","S00019171":"28.14510136"},{"Metabolite":"L-ORNITHINE","S00019157":"18.39144708","S00019158":"17.81013858","S00019159":"22.81921146","S00019160":"26.59392455","S00019161":"16.5173662","S00019162":"20.90979775","S00019163":"23.31567812","S00019164":"32.74355375","S00019165":"23.67462381","S00019166":"7.174070822","S00019167":"9.291184644","S00019168":"10.43377154","S00019169":"13.1935425","S00019170":"31.32135599","S00019171":"17.22311005"},{"Metabolite":"L-ASPARAGINE","S00019157":"17.63197989","S00019158":"4.796622517","S00019159":"14.42740896","S00019160":"19.3969766","S00019161":"9.443504931","S00019162":"8.239099988","S00019163":"16.26814967","S00019164":"21.79670289","S00019165":"22.36888011","S00019166":"","S00019167":"","S00019168":"8.56159894","S00019169":"5.676498381","S00019170":"20.99813139","S00019171":"15.5186241"},{"Metabolite":"L-VALINE","S00019157":"143.9964676","S00019158":"83.17030003","S00019159":"97.51919296","S00019160":"127.9290484","S00019161":"91.70798867","S00019162":"107.4847824","S00019163":"177.6695613","S00019164":"162.822852","S00019165":"317.0808714","S00019166":"77.58234244","S00019167":"85.27751392","S00019168":"107.2768937","S00019169":"115.8897173","S00019170":"271.4082419","S00019171":"267.0590075"},{"Metabolite":"L-THREONINE","S00019157":"57.89627107","S00019158":"39.92979857","S00019159":"69.58040576","S00019160":"72.32522422","S00019161":"46.11105868","S00019162":"46.08079285","S00019163":"56.05549794","S00019164":"72.51648847","S00019165":"88.52513772","S00019166":"44.76371671","S00019167":"37.69116282","S00019168":"44.21232159","S00019169":"39.31510408","S00019170":"88.3940365","S00019171":"77.15415444"},{"Metabolite":"L-TRYPTOPHAN","S00019157":"3.11230765","S00019158":"12.21336199","S00019159":"14.46289428","S00019160":"14.30781344","S00019161":"12.31672365","S00019162":"5.800346247","S00019163":"11.70403374","S00019164":"8.462933989","S00019165":"23.84368811","S00019166":"9.263910066","S00019167":"4.647104526","S00019168":"4.870037851","S00019169":"6.312381775","S00019170":"6.156957908","S00019171":"4.388855904"},{"Metabolite":"L-ISOLEUCINE","S00019157":"52.88728818","S00019158":"28.28398609","S00019159":"33.39795773","S00019160":"45.4471672","S00019161":"36.74269305","S00019162":"45.09738448","S00019163":"67.35088447","S00019164":"76.494299","S00019165":"130.0657661","S00019166":"36.42551801","S00019167":"35.64650536","S00019168":"39.71537283","S00019169":"38.01355057","S00019170":"141.7818523","S00019171":"94.73362123"},{"Metabolite":"GLYCINE","S00019157":"92.76042683","S00019158":"68.27596575","S00019159":"112.9803821","S00019160":"113.3042751","S00019161":"89.56905547","S00019162":"74.53526431","S00019163":"81.48283244","S00019164":"78.36549166","S00019165":"115.8635949","S00019166":"71.34582346","S00019167":"59.76798149","S00019168":"77.86288643","S00019169":"71.8500794","S00019170":"96.28999921","S00019171":"106.0350383"},{"Metabolite":"4-HYDROXYPROLINE","S00019157":"3.903788138","S00019158":"4.192759241","S00019159":"5.317962921","S00019160":"3.751833708","S00019161":"","S00019162":"3.25562901","S00019163":"2.405008929","S00019164":"3.883884211","S00019165":"3.691598591","S00019166":"1.202856351","S00019167":"1.374326002","S00019168":"4.509427697","S00019169":"3.73136959","S00019170":"8.204177141","S00019171":"8.003231897"}], "Metabolites":[{"Metabolite":"L-PROLINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"145742","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-GLUTAMIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"33032","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-ALANINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5950","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-SERINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5951","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-ASPARTIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5960","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-GLUTAMINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5961","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-LYSINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5962","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-TYROSINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6057","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-LEUCINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6106","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"2-AMINOISOBUTYRIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6119","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-METHIONINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6137","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-PHENYLALANINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6140","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-ORNITHINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6262","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-ASPARAGINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6267","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-VALINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6287","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-THREONINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6288","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-TRYPTOPHAN","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6305","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-ISOLEUCINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6306","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"GLYCINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"750","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"4-HYDROXYPROLINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"825","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""}] } }