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The interpretability of our results demonstrates the promise of using genetic variants underlying differences in the metabolome to predict novel phenotypes from genotype.","INSTITUTE":"Washington University in St. Louis","LAST_NAME":"Swain Lenz","FIRST_NAME":"Devjanee","ADDRESS":"4515 McKinley Avenue, Saint Louis, Missouri, 63110, USA","EMAIL":"devjanee.swain.lenz@duke.edu","PHONE":"314-362-3679"}, "STUDY":{"STUDY_TITLE":"Untargeted metabolomic profile of Saccharomyces cerevisiae (F2) hybrids (part II)","STUDY_SUMMARY":"Metabolomic profiles were compiled from oak and wine yeast parents, and their F2 hybrids. 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"Sample ID":"STD_7b", "Factors":{"Parent":"BC233_BC240_mix"}, "Additional sample data":{"batch":"1"} }, { "Subject ID":"BC233_BC240_mix", "Sample ID":"STD_7c", "Factors":{"Parent":"BC233_BC240_mix"}, "Additional sample data":{"batch":"1"} }, { "Subject ID":"BC233_BC240_mix", "Sample ID":"STD_8", "Factors":{"Parent":"BC233_BC240_mix"}, "Additional sample data":{"batch":"1"} }, { "Subject ID":"BC233_BC240_mix", "Sample ID":"STD_8b", "Factors":{"Parent":"BC233_BC240_mix"}, "Additional sample data":{"batch":"1"} }, { "Subject ID":"BC233_BC240_mix", "Sample ID":"STD_8c", "Factors":{"Parent":"BC233_BC240_mix"}, "Additional sample data":{"batch":"1"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Cells were grown at 30°C in synthetic dextrose media (0.145% yeast nitrogen base minus amino acids/ammonium sulfate, 0.5% ammonium sulfate, 2% dextrose) to an OD of 0.55 - 0.65. We harvested cells by vacuum filter."}, "TREATMENT":{"TREATMENT_SUMMARY":"Cells were grown at 30°C in synthetic dextrose media (0.145% yeast nitrogen base minus amino acids/ammonium sulfate, 0.5% ammonium sulfate, 2% dextrose) to an OD of 0.55 - 0.65. Different genotypes were used, and media was made at three separate times (batch)."}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"We harvested cells by vacuum filter, and extracted hydrophilic metabolites from 0.2 um filters using 40:40:20% (v/v/v) methanol/acetonitrile/water8. We froze and thawed extracts at -80 °C and -20 °C, respectively, three times. We pelleted cells, and stored the supernatant at -80 °C until we performed mass spectrometry"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"None (Direct infusion)","INSTRUMENT_NAME":"None","COLUMN_NAME":"none"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Thermo LTQ Discovery Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"API","ION_MODE":"NEGATIVE","MS_RESULTS_FILE":"ST000870_AN001417_Results.txt UNITS:normalized peak intensity"} }