{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000954","ANALYSIS_ID":"AN001565","VERSION":"1","CREATED_ON":"April 18, 2018, 4:44 pm"}, "PROJECT":{"PROJECT_TITLE":"Using Metabolomics and Lipidomics Analysis to Explore Metabolites and Pathways Associated Increased Airway Hyperresponsiveness in Patients with Asthma who are Identified by Race (African American) and Genotype (ADRB2 Arg16/Arg)","PROJECT_TYPE":"Open-label, prospective corhort study","PROJECT_SUMMARY":"Asthma is a heterogeneous disease largely defined by chronic airway inflammation with similar symptomatology in patients that includes wheezing, shortness of breath, chest tightness and cough. However, underlying these common symptoms are varying endotypes with distinct pathophysiological processes. Metabolomic studies in patients with asthma are emerging and suggest that metabolomics can characterize distinct asthma phenotypes. In a completed study, we identified a population of patients with asthma who have increased airway hyperresponsiveness (airway hyperresponsiveness is a marker for asthma disease severity) who are characterized by race (African American) and genotype (ADRB2 Arg16/Arg) compared with patients who have less airway hyperresponsiveness (African Americans and whites with differing ADRB2 genotypes). This group may represent a distinct endotype of asthma with unique metabolomic and lipidomic characteristics. The aims of this project are to (1) use metabolomic and lipidomic analysis to identify metabolites present in plasma in this population of patients with asthma who have increased airway hyperresponsiveness (African Americans who carry the ADRB2 Arg16/Arg genotype) and patients with asthma who have less airway hyperresponsiveness (African Americans and whites with differing ADRB2 genotypes); and (2) identify pathways that will improve the understanding of increased airway hyperresponsiveness in this population. We hypothesize that there will be unique metabolic pathways in the population with increased airway hyperresponsiveness that will be distinct from pathways in patients with lower airway hyperresponsiveness. In this project will use data and samples that were previously collected as part of the NIH funded project “Pharmacogenetics of β2-Agonists in Asthma” (Blake, PI K23 HL081245). Blood was collected in 55 African Americans and whites after receiving 2-weeks treatment with inhaled fluticasone. Samples were stored on ice until processed and plasma frozen at -80°C. If our findings indicate distinct metabolic pathways are present using global metabolomic and lipodomic analysis, we will seek to replicate our findings using samples and data from phenotypically well characterized participants who participated in trials conducted through the American Lung Association Airways Clinical Research Centers network, of which Nemours has been a highly productive site since 1999. Future controlled trials would be conducted to evaluate treatments based upon molecular pathways identified through metabolomic and lipidomic analysis.","INSTITUTE":"Nemours Children's Specialty Care","DEPARTMENT":"Center for Pharmacogenomics and Translational Research","LABORATORY":"Pulmonology laboratory","LAST_NAME":"Blake","FIRST_NAME":"Kathryn","ADDRESS":"807 Childrens's Way Jacksonville, Florida 32207","EMAIL":"Kathryn.Blake@nemours.org","PHONE":"(904) 697-3806","FUNDING_SOURCE":"SECIM pilot and feasibility, NIH U24 DK097209"}, "STUDY":{"STUDY_TITLE":"Using Metabolomics and Lipidomics Analysis to Explore Metabolites and Pathways Associated Increased Airway Hyperresponsiveness in Patients with Asthma who are Identified by Race (African American) and Genotype (ADRB2 Arg16/Arg)","STUDY_TYPE":"Open-label, prospective cohort study","STUDY_SUMMARY":"Asthma is a heterogeneous disease largely defined by chronic airway inflammation with similar symptomatology in patients that includes wheezing, shortness of breath, chest tightness and cough. However, underlying these common symptoms are varying endotypes with distinct pathophysiological processes. Metabolomic studies in patients with asthma are emerging and suggest that metabolomics can characterize distinct asthma phenotypes. In a completed study, we identified a population of patients with asthma who have increased airway hyperresponsiveness (airway hyperresponsiveness is a marker for asthma disease severity) who are characterized by race (African American) and genotype (ADRB2 Arg16/Arg) compared with patients who have less airway hyperresponsiveness (African Americans and whites with differing ADRB2 genotypes). This group may represent a distinct endotype of asthma with unique metabolomic and lipidomic characteristics. The aims of this project are to (1) use metabolomic and lipidomic analysis to identify metabolites present in plasma in this population of patients with asthma who have increased airway hyperresponsiveness (African Americans who carry the ADRB2 Arg16/Arg genotype) and patients with asthma who have less airway hyperresponsiveness (African Americans and whites with differing ADRB2 genotypes); and (2) identify pathways that will improve the understanding of increased airway hyperresponsiveness in this population. We hypothesize that there will be unique metabolic pathways in the population with increased airway hyperresponsiveness that will be distinct from pathways in patients with lower airway hyperresponsiveness. In this project will use data and samples that were previously collected as part of the NIH funded project “Pharmacogenetics of β2-Agonists in Asthma” (Blake, PI K23 HL081245). Blood was collected in 55 African Americans and whites after receiving 2-weeks treatment with inhaled fluticasone. Samples were stored on ice until processed and plasma frozen at -80°C. If our findings indicate distinct metabolic pathways are present using global metabolomic and lipodomic analysis, we will seek to replicate our findings using samples and data from phenotypically well characterized participants who participated in trials conducted through the American Lung Association Airways Clinical Research Centers network, of which Nemours has been a highly productive site since 1999. Future controlled trials would be conducted to evaluate treatments based upon molecular pathways identified through metabolomic and lipidomic analysis.","INSTITUTE":"University of Florida","DEPARTMENT":"SECIM","LAST_NAME":"Beecher","FIRST_NAME":"Chris","ADDRESS":"PO Box 100219 Gainesville FL 32610-0219 , Southeast Center for Integrated Metabolomics","EMAIL":"chris@iroatech.com","PHONE":"(352) 294-4385","NUM_GROUPS":"4","TOTAL_SUBJECTS":"55","STUDY_COMMENTS":"Nubmer of groups : 4 (race x diplotype); SECIM pilot and feasibility, NIH U24 DK097209"}, "SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","AGE_OR_AGE_RANGE":"11-67 yo","WEIGHT_OR_WEIGHT_RANGE":"32-194 kg","HEIGHT_OR_HEIGHT_RANGE":"141-388 cm","GENDER":"Male and female","HUMAN_RACE":"47% white, 53% african american","HUMAN_ETHNICITY":"95% not Hispanic","HUMAN_TRIAL_TYPE":"Open-label, prospective cohort study","HUMAN_LIFESTYLE_FACTORS":"NA","HUMAN_MEDICATIONS":"fluticasone (was not the intervention in this project, all participants received this drug for 2 weeks prior to sample collection)","HUMAN_PRESCRIPTION_OTC":"prescription","HUMAN_SMOKING_STATUS":"9% current, 20% former"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"068-K23NIH-120", "Sample ID":"13779184", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"1","daily_dose":"3"}, "Additional sample data":{"age":"61.4","fev1_pct":"52","pc20":"2.3784"} }, { "Subject ID":"126-K23NIH-121", "Sample ID":"13779193", "Factors":{"race":"1","diplotype":"1","ethnicity":"1","ever_smoked":"1","daily_dose":"2"}, "Additional sample data":{"age":"40.2","fev1_pct":"75","pc20":"0.8942"} }, { "Subject ID":"331-K23NIH-122", "Sample ID":"13779201", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"4"}, "Additional sample data":{"age":"64.9","fev1_pct":"85","pc20":"5.0397"} }, { "Subject ID":"128-K23NIH-123", "Sample ID":"13779209", "Factors":{"race":"1","diplotype":"1","ethnicity":"2","ever_smoked":"1","daily_dose":"2"}, "Additional sample data":{"age":"56.4","fev1_pct":"88","pc20":"32"} }, { "Subject ID":"115-K23NIH-124", "Sample ID":"13779234", "Factors":{"race":"1","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"63","fev1_pct":"83","pc20":"5.1974"} }, { "Subject ID":"157-K23NIH-128", "Sample ID":"13779260", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"15.9","fev1_pct":"95","pc20":"6.8302"} }, { "Subject ID":"159-K23NIH-129", "Sample ID":"13779270", "Factors":{"race":"2","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"13.1","fev1_pct":"101","pc20":"0.7895"} }, { "Subject ID":"022-K23NIH-130", "Sample ID":"13765456", "Factors":{"race":"1","diplotype":"1","ethnicity":"1","ever_smoked":"1","daily_dose":"2"}, "Additional sample data":{"age":"25.9","fev1_pct":"102","pc20":"0.6507"} }, { "Subject ID":"169-K23NIH-131", "Sample ID":"13765466", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"1","daily_dose":"4"}, "Additional sample data":{"age":"57.9","fev1_pct":"70","pc20":"4"} }, { "Subject ID":"170-K23NIH-132", "Sample ID":"13765511", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"66.5","fev1_pct":"95","pc20":"32"} }, { "Subject ID":"149-K23NIH-133", "Sample ID":"13765520", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"20.3","fev1_pct":"81","pc20":"3.3543"} }, { "Subject ID":"077-K23NIH-134", "Sample ID":"13765529", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"14.3","fev1_pct":"105","pc20":"32"} }, { "Subject ID":"175-K23NIH-135", "Sample ID":"13765547", "Factors":{"race":"2","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"18.6","fev1_pct":"99","pc20":"0.5453"} }, { "Subject ID":"013-K23NIH-137", "Sample ID":"13765863", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"4"}, "Additional sample data":{"age":"29.3","fev1_pct":"67","pc20":"1.5376"} }, { "Subject ID":"316-K23NIH-139", "Sample ID":"13765893", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"1","daily_dose":"3"}, "Additional sample data":{"age":"54","fev1_pct":"62","pc20":"5.6569"} }, { "Subject ID":"042-K23NIH-140", "Sample ID":"13765902", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"1","daily_dose":"4"}, "Additional sample data":{"age":"19.6","fev1_pct":"73","pc20":"0.1646"} }, { "Subject ID":"180-K23NIH-141", "Sample ID":"13765925", "Factors":{"race":"1","diplotype":"2","ethnicity":"-","ever_smoked":"2","daily_dose":"3"}, "Additional sample data":{"age":"64.8","fev1_pct":"68","pc20":"1.7411"} }, { "Subject ID":"037-K23NIH-143", "Sample ID":"13765368", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"3"}, "Additional sample data":{"age":"14.3","fev1_pct":"108","pc20":"32"} }, { "Subject ID":"196-K23NIH-144", "Sample ID":"13765377", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"1","daily_dose":"2"}, "Additional sample data":{"age":"22.1","fev1_pct":"66","pc20":"7.0168"} }, { "Subject ID":"357-K23NIH-145", "Sample ID":"13765395", "Factors":{"race":"2","diplotype":"1","ethnicity":"2","ever_smoked":"1","daily_dose":"4"}, "Additional sample data":{"age":"24.1","fev1_pct":"90","pc20":"0.5304"} }, { "Subject ID":"182-K23NIH-147", "Sample ID":"13765414", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"65.8","fev1_pct":"89","pc20":"2.1301"} }, { "Subject ID":"359-K23NIH-149", "Sample ID":"13765432", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"4"}, "Additional sample data":{"age":"43.9","fev1_pct":"107","pc20":"5.8709"} }, { "Subject ID":"177-K23NIH-148", "Sample ID":"13765440", "Factors":{"race":"1","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"3"}, "Additional sample data":{"age":"27.6","fev1_pct":"111","pc20":"32"} }, { "Subject ID":"204-K23NIH-151", "Sample ID":"13765745", "Factors":{"race":"1","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"37.7","fev1_pct":"91","pc20":"0.933"} }, { "Subject ID":"219-K23NIH-152", "Sample ID":"13765773", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"1","daily_dose":"2"}, "Additional sample data":{"age":"50","fev1_pct":"57","pc20":"0.3015"} }, { "Subject ID":"208-K23NIH-155", "Sample ID":"13765807", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"45.9","fev1_pct":"87","pc20":"1.3704"} }, { "Subject ID":"186-K23NIH-156", "Sample ID":"13765815", "Factors":{"race":"1","diplotype":"2","ethnicity":"1","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"27.4","fev1_pct":"89","pc20":"0.6177"} }, { "Subject ID":"222-K23NIH-157", "Sample ID":"13765265", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"11.1","fev1_pct":"82","pc20":"3.4205"} }, { "Subject ID":"141-K23NIH-158", "Sample ID":"13765274", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"1"}, "Additional sample data":{"age":"19.6","fev1_pct":"73","pc20":"0.0666"} }, { "Subject ID":"012-K23NIH-159", "Sample ID":"13765281", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"1","daily_dose":"2"}, "Additional sample data":{"age":"60.9","fev1_pct":"64","pc20":"5.8633"} }, { "Subject ID":"274-K23NIH-160", "Sample ID":"13765289", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"3"}, "Additional sample data":{"age":"24.1","fev1_pct":"60","pc20":"0.6095"} }, { "Subject ID":"278-K23NIH-162", "Sample ID":"13765316", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"3"}, "Additional sample data":{"age":"39.8","fev1_pct":"86","pc20":"32"} }, { "Subject ID":"043-K23NIH-161", "Sample ID":"13765325", "Factors":{"race":"2","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"3"}, "Additional sample data":{"age":"16.1","fev1_pct":"101","pc20":"32"} }, { "Subject ID":"256-K23NIH-163", "Sample ID":"13765354", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"13.6","fev1_pct":"93","pc20":"0.2299"} }, { "Subject ID":"198-K23NIH-164", "Sample ID":"13765181", "Factors":{"race":"2","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"3"}, "Additional sample data":{"age":"10.9","fev1_pct":"92","pc20":"0.6182"} }, { "Subject ID":"259-K23NIH-165", "Sample ID":"13765207", "Factors":{"race":"2","diplotype":"1","ethnicity":"2","ever_smoked":"1","daily_dose":"2"}, "Additional sample data":{"age":"58.3","fev1_pct":"62","pc20":"0.743"} }, { "Subject ID":"257-K23NIH-166", "Sample ID":"13765227", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"1","daily_dose":"2"}, "Additional sample data":{"age":"63.2","fev1_pct":"72","pc20":"32"} }, { "Subject ID":"282-K23NIH-167", "Sample ID":"13765247", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"15.2","fev1_pct":"108","pc20":"5.5459"} }, { "Subject ID":"299-K23NIH-168", "Sample ID":"13765659", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"32.1","fev1_pct":"108","pc20":"32"} }, { "Subject ID":"016-K23NIH-171", "Sample ID":"13765668", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"1","daily_dose":"2"}, "Additional sample data":{"age":"47.7","fev1_pct":"81","pc20":"0.315"} }, { "Subject ID":"500-K23NIH-169", "Sample ID":"13765678", "Factors":{"race":"1","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"18.3","fev1_pct":"102","pc20":"4.514"} }, { "Subject ID":"058-K23NIH-172", "Sample ID":"13765705", "Factors":{"race":"1","diplotype":"1","ethnicity":"2","ever_smoked":"1","daily_dose":"2"}, "Additional sample data":{"age":"20.5","fev1_pct":"88","pc20":"0.5215"} }, { "Subject ID":"293-K23NIH-173", "Sample ID":"13765714", "Factors":{"race":"2","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"3"}, "Additional sample data":{"age":"10.9","fev1_pct":"75","pc20":"2"} }, { "Subject ID":"504-K23NIH-174", "Sample ID":"13765561", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"10.8","fev1_pct":"85","pc20":"0.8052"} }, { "Subject ID":"244-K23NIH-175", "Sample ID":"13765579", "Factors":{"race":"2","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"29.49","fev1_pct":"84","pc20":"0.7492"} }, { "Subject ID":"531-K23NIH-178", "Sample ID":"13765605", "Factors":{"race":"1","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"13.3","fev1_pct":"106","pc20":"32"} }, { "Subject ID":"529-K23NIH-177", "Sample ID":"13765615", "Factors":{"race":"1","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"11.7","fev1_pct":"82","pc20":"12.0273"} }, { "Subject ID":"539-K23NIH-180", "Sample ID":"13765635", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"1","daily_dose":"3"}, "Additional sample data":{"age":"16","fev1_pct":"100","pc20":"0.4674"} }, { "Subject ID":"547-K23NIH-182", "Sample ID":"13765645", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"14.77","fev1_pct":"114","pc20":"3.387"} }, { "Subject ID":"541-K23NIH-181", "Sample ID":"13765079", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"30.75","fev1_pct":"80","pc20":"32"} }, { "Subject ID":"251-K23NIH-183", "Sample ID":"13765107", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"10.28","fev1_pct":"102","pc20":"6.7272"} }, { "Subject ID":"536-K23NIH-185", "Sample ID":"13765118", "Factors":{"race":"2","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"15.72","fev1_pct":"103","pc20":"1.2434"} }, { "Subject ID":"533-K23NIH-184", "Sample ID":"13765124", "Factors":{"race":"2","diplotype":"2","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"14.63","fev1_pct":"87","pc20":"1.7632"} }, { "Subject ID":"291-K23NIH-186", "Sample ID":"13765162", "Factors":{"race":"2","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"11.83","fev1_pct":"88","pc20":"32"} }, { "Subject ID":"553-K23NIH-188", "Sample ID":"13774973", "Factors":{"race":"2","diplotype":"1","ethnicity":"2","ever_smoked":"2","daily_dose":"2"}, "Additional sample data":{"age":"14.53","fev1_pct":"81","pc20":"1.486"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Whole blood is collected in an EDTA vacutainer at Visit 2 after two weeks of fluticasone therapy. 1 ml aliquots at 80°C","SAMPLE_TYPE":"Blood (whole)","COLLECTION_METHOD":"Whole blood is collected in an EDTA vacutainer (Visit 2; after two weeks of fluticasone therapy) and is stored on ice. Within 2 hr of collection, the sample is centrifuged at 2000 rpm in a 4°C refrigerated benchtop centrifuge and the plasma is recovered and stored in aliquots at -80°C.","COLLECTION_LOCATION":"Nemours Children's Clinic Phlebotomy Laboratory.","COLLECTION_FREQUENCY":"One time","STORAGE_CONDITIONS":"Described in summary","COLLECTION_VIALS":"10 ml potassium EDTA vacutainer","STORAGE_VIALS":"1.4 ml Micronic unthreaded 2D data matrix tubes with TPE push caps","COLLECTION_TUBE_TEMP":"4°C","ADDITIVES":"potassium EDTA"}, "TREATMENT":{"TREATMENT_SUMMARY":"All subjects will be treated with at least 110 mg of fluticasone propionate once or twice daily (Flovent® MDI) during the 2-week treatment period.","TREATMENT_PROTOCOL_ID":"NCT00708227","TREATMENT_PROTOCOL_COMMENTS":"Note: Fluticasone was not the intervention in this project; all study participants received this drug for 2 weeks prior to blood collection for the sample being used for the metabolomic analysis. However, participants did receive different doses of fluticasone in order to have them remain on the same dose that they were on prior to study entry. This was to ensure that their asthma remained stable at the end of the 2 week treatment with fluticasone.","TREATMENT_COMPOUND":"fluticasone","TREATMENT_ROUTE":"nebulizer; inhaled","TREATMENT_DOSE":"110 mcg, 220 mcg, 440 mcg, 880 mcg","TREATMENT_DOSEDURATION":"daily"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"none","SAMPLEPREP_PROTOCOL_FILENAME":"GMetabolomics_LCMS_Protocol_092117.pdf;Appendix_A_Internal_Standard_Prep_GLCMS.pdf"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)","FLOW_RATE":"350 ul/min","SOLVENT_A":"0.1% Formic Acid in Water","SOLVENT_B":"Acetonitrile"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_RESULTS_FILE":"ST000954_AN001565_Results.txt UNITS:Peak height"}, "MS_METABOLITE_DATA":{ "Units":"peak height", 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