#METABOLOMICS WORKBENCH sean_harshman_20181008_073832 DATATRACK_ID:1530 STUDY_ID:ST001068 ANALYSIS_ID:AN001748 PROJECT_ID:PR000715 VERSION 1 CREATED_ON October 9, 2018, 5:31 pm #PROJECT PR:PROJECT_TITLE The proteomic and metabolomic characterization of exercise-induced sweat PR:PROJECT_SUMMARY Proteomic and Metabolomic analysis of exercise-induced sweat to evaluate analyte PR:PROJECT_SUMMARY correlation with human performance parameters. PR:INSTITUTE UES Inc, Air Force Research Laboratory PR:LAST_NAME Harshman PR:FIRST_NAME Sean PR:ADDRESS 2510 Fifth Street, Area B, Building 840, WPAFB, OH 45433 PR:EMAIL sean.harshman.ctr@us.af.mil PR:PHONE 937-938-3785 #STUDY ST:STUDY_TITLE The proteomic and metabolomic characterization of exercise-induced sweat (part ST:STUDY_TITLE -I) ST:STUDY_SUMMARY Proteomic and Metabolomic analysis of exercise-induced sweat to evaluate analyte ST:STUDY_SUMMARY correlation with human performance parameters. Sweat was collected from ST:STUDY_SUMMARY participants on a treadmill at low, medium, and hard speed and incline. Sweat ST:STUDY_SUMMARY was analyzed by untagged HILIC LC-MS. ST:INSTITUTE UES Inc, Air Force Research Laboratory ST:LAST_NAME Harshman ST:FIRST_NAME Sean ST:ADDRESS 2510 Fifth Street, Area B, Building 840, WPAFB, OH 45433 ST:EMAIL sean.harshman.ctr@us.af.mil ST:PHONE 937-938-3785 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - Subject_#13_Positive_HILIC__01 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#13_Positive_HILIC__02 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#14_Positive_HILIC__01 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#14_Positive_HILIC__02 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#15_Positive_HILIC_01 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#15_Positive_HILIC_02 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#16_Positive_HILIC__01 Treadmill Speed:Hard SUBJECT_SAMPLE_FACTORS - Subject_#16_Positive_HILIC__02 Treadmill Speed:Hard SUBJECT_SAMPLE_FACTORS - Subject_#17_Positive_HILIC_01 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#17_Positive_HILIC_02 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#19_Positive_HILIC__01 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#19_Positive_HILIC__02 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#20_Positive_HILIC_01 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#20_Positive_HILIC_02 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#21_Positive_HILIC_01 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#21_Positive_HILIC_02 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#23_Positive_HILIC__01 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#23_Positive_HILIC__02 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#24_Positive_HILIC_01 Treadmill Speed:Hard SUBJECT_SAMPLE_FACTORS - Subject_#24_Positive_HILIC_02 Treadmill Speed:Hard SUBJECT_SAMPLE_FACTORS - Pool_Positive_01 Treadmill Speed:Pool SUBJECT_SAMPLE_FACTORS - Pool_Positive_02 Treadmill Speed:Pool SUBJECT_SAMPLE_FACTORS - Pool_Positive_03 Treadmill Speed:Pool SUBJECT_SAMPLE_FACTORS - Pool_Positive_04 Treadmill Speed:Pool SUBJECT_SAMPLE_FACTORS - Pool_Positive_05 Treadmill Speed:Pool SUBJECT_SAMPLE_FACTORS - Subject_#13_Negative_HILIC_01 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#13_Negative_HILIC_02 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#14_Negative_HILIC_01 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#14_Negative_HILIC_02 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#15_Negative_HILIC_01 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#15_Negative_HILIC_02 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#16_Negative_HILIC_01 Treadmill Speed:Hard SUBJECT_SAMPLE_FACTORS - Subject_#16_Negative_HILIC_02 Treadmill Speed:Hard SUBJECT_SAMPLE_FACTORS - Subject_#17_Negative_HILIC_01 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#17_Negative_HILIC_02 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#19_Negative_HILIC_01 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#19_Negative_HILIC_02 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#20_Negative_HILIC_01 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#20_Negative_HILIC_02 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#21_Negative_HILIC_01 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#21_Negative_HILIC_02 Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#23_Negative_HILIC_01 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#23_Negative_HILIC_02 Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#24_Negative_HILIC_01 Treadmill Speed:Hard SUBJECT_SAMPLE_FACTORS - Subject_#24_Negative_HILIC_02 Treadmill Speed:Hard SUBJECT_SAMPLE_FACTORS - Pool_Negative_01 Treadmill Speed:Pool SUBJECT_SAMPLE_FACTORS - Pool_Negative_02 Treadmill Speed:Pool SUBJECT_SAMPLE_FACTORS - Pool_Negative_03 Treadmill Speed:Pool SUBJECT_SAMPLE_FACTORS - Pool_Negative_04 Treadmill Speed:Pool SUBJECT_SAMPLE_FACTORS - Pool_Negative_05 Treadmill Speed:Pool #COLLECTION CO:COLLECTION_SUMMARY Sweat collected from the forearms of participants. CO:SAMPLE_TYPE Sweat #TREATMENT TR:TREATMENT_SUMMARY Sweat induced by exercise on a treadmill. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Sweat samples were collected, pooled, and frozen on liquid nitrogen. Following, SP:SAMPLEPREP_SUMMARY frozen samples were lyophilized and frozen until analysis. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 CH:COLUMN_NAME Phenomenex Luna NH2 (100 x 3mm, 3um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Thermo LTQ XL MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_RESULTS_FILE ST001068_AN001748_Results.txt UNITS:Abundance #END