{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001068","ANALYSIS_ID":"AN001749","VERSION":"1","CREATED_ON":"October 9, 2018, 5:31 pm"},

"PROJECT":{"PROJECT_TITLE":"The proteomic and metabolomic characterization of exercise-induced sweat","PROJECT_SUMMARY":"Proteomic and Metabolomic analysis of exercise-induced sweat to evaluate analyte correlation with human performance parameters.","INSTITUTE":"UES Inc, Air Force Research Laboratory","LAST_NAME":"Harshman","FIRST_NAME":"Sean","ADDRESS":"2510 Fifth Street, Area B, Building 840, WPAFB, OH 45433","EMAIL":"sean.harshman.ctr@us.af.mil","PHONE":"937-938-3785"},

"STUDY":{"STUDY_TITLE":"The proteomic and metabolomic characterization of exercise-induced sweat (part -I)","STUDY_SUMMARY":"Proteomic and Metabolomic analysis of exercise-induced sweat to evaluate analyte correlation with human performance parameters. Sweat was collected from participants on a treadmill at low, medium, and hard speed and incline. Sweat was analyzed by untagged HILIC LC-MS.","INSTITUTE":"UES Inc, Air Force Research Laboratory","LAST_NAME":"Harshman","FIRST_NAME":"Sean","ADDRESS":"2510 Fifth Street, Area B, Building 840, WPAFB, OH 45433","EMAIL":"sean.harshman.ctr@us.af.mil","PHONE":"937-938-3785"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Subject_#13_Positive_HILIC__01",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#13_Positive_HILIC__02",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#14_Positive_HILIC__01",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#14_Positive_HILIC__02",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#15_Positive_HILIC_01",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#15_Positive_HILIC_02",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#16_Positive_HILIC__01",
"Factors":{"Treadmill Speed":"Hard"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#16_Positive_HILIC__02",
"Factors":{"Treadmill Speed":"Hard"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#17_Positive_HILIC_01",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#17_Positive_HILIC_02",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#19_Positive_HILIC__01",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#19_Positive_HILIC__02",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#20_Positive_HILIC_01",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#20_Positive_HILIC_02",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#21_Positive_HILIC_01",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#21_Positive_HILIC_02",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#23_Positive_HILIC__01",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#23_Positive_HILIC__02",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#24_Positive_HILIC_01",
"Factors":{"Treadmill Speed":"Hard"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#24_Positive_HILIC_02",
"Factors":{"Treadmill Speed":"Hard"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_Positive_01",
"Factors":{"Treadmill Speed":"Pool"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_Positive_02",
"Factors":{"Treadmill Speed":"Pool"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_Positive_03",
"Factors":{"Treadmill Speed":"Pool"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_Positive_04",
"Factors":{"Treadmill Speed":"Pool"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_Positive_05",
"Factors":{"Treadmill Speed":"Pool"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#13_Negative_HILIC_01",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#13_Negative_HILIC_02",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#14_Negative_HILIC_01",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#14_Negative_HILIC_02",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#15_Negative_HILIC_01",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#15_Negative_HILIC_02",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#16_Negative_HILIC_01",
"Factors":{"Treadmill Speed":"Hard"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#16_Negative_HILIC_02",
"Factors":{"Treadmill Speed":"Hard"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#17_Negative_HILIC_01",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#17_Negative_HILIC_02",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#19_Negative_HILIC_01",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#19_Negative_HILIC_02",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#20_Negative_HILIC_01",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#20_Negative_HILIC_02",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#21_Negative_HILIC_01",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#21_Negative_HILIC_02",
"Factors":{"Treadmill Speed":"Low"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#23_Negative_HILIC_01",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#23_Negative_HILIC_02",
"Factors":{"Treadmill Speed":"Medium"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#24_Negative_HILIC_01",
"Factors":{"Treadmill Speed":"Hard"}
},
{
"Subject ID":"-",
"Sample ID":"Subject_#24_Negative_HILIC_02",
"Factors":{"Treadmill Speed":"Hard"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_Negative_01",
"Factors":{"Treadmill Speed":"Pool"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_Negative_02",
"Factors":{"Treadmill Speed":"Pool"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_Negative_03",
"Factors":{"Treadmill Speed":"Pool"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_Negative_04",
"Factors":{"Treadmill Speed":"Pool"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_Negative_05",
"Factors":{"Treadmill Speed":"Pool"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Sweat collected from the forearms of participants.","SAMPLE_TYPE":"Sweat"},

"TREATMENT":{"TREATMENT_SUMMARY":"Sweat induced by exercise on a treadmill."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Sweat samples were collected, pooled, and frozen on liquid nitrogen. Following, frozen samples were lyophilized and frozen until analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"Phenomenex Luna NH2 (100 x 3mm, 3um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Thermo LTQ XL","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_RESULTS_FILE":"ST001068_AN001749_Results.txt UNITS:Abundance"}

}