{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001068","ANALYSIS_ID":"AN001749","VERSION":"1","CREATED_ON":"October 9, 2018, 5:31 pm"}, "PROJECT":{"PROJECT_TITLE":"The proteomic and metabolomic characterization of exercise-induced sweat","PROJECT_SUMMARY":"Proteomic and Metabolomic analysis of exercise-induced sweat to evaluate analyte correlation with human performance parameters.","INSTITUTE":"UES Inc, Air Force Research Laboratory","LAST_NAME":"Harshman","FIRST_NAME":"Sean","ADDRESS":"2510 Fifth Street, Area B, Building 840, WPAFB, OH 45433","EMAIL":"sean.harshman.ctr@us.af.mil","PHONE":"937-938-3785"}, "STUDY":{"STUDY_TITLE":"The proteomic and metabolomic characterization of exercise-induced sweat (part -I)","STUDY_SUMMARY":"Proteomic and Metabolomic analysis of exercise-induced sweat to evaluate analyte correlation with human performance parameters. Sweat was collected from participants on a treadmill at low, medium, and hard speed and incline. Sweat was analyzed by untagged HILIC LC-MS.","INSTITUTE":"UES Inc, Air Force Research Laboratory","LAST_NAME":"Harshman","FIRST_NAME":"Sean","ADDRESS":"2510 Fifth Street, Area B, Building 840, WPAFB, OH 45433","EMAIL":"sean.harshman.ctr@us.af.mil","PHONE":"937-938-3785"}, "SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"Subject_#13_Positive_HILIC__01", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#13_Positive_HILIC__02", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#14_Positive_HILIC__01", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#14_Positive_HILIC__02", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#15_Positive_HILIC_01", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#15_Positive_HILIC_02", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#16_Positive_HILIC__01", "Factors":{"Treadmill Speed":"Hard"} }, { "Subject ID":"-", "Sample ID":"Subject_#16_Positive_HILIC__02", "Factors":{"Treadmill Speed":"Hard"} }, { "Subject ID":"-", "Sample ID":"Subject_#17_Positive_HILIC_01", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#17_Positive_HILIC_02", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#19_Positive_HILIC__01", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#19_Positive_HILIC__02", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#20_Positive_HILIC_01", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#20_Positive_HILIC_02", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#21_Positive_HILIC_01", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#21_Positive_HILIC_02", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#23_Positive_HILIC__01", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#23_Positive_HILIC__02", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#24_Positive_HILIC_01", "Factors":{"Treadmill Speed":"Hard"} }, { "Subject ID":"-", "Sample ID":"Subject_#24_Positive_HILIC_02", "Factors":{"Treadmill Speed":"Hard"} }, { "Subject ID":"-", "Sample ID":"Pool_Positive_01", "Factors":{"Treadmill Speed":"Pool"} }, { "Subject ID":"-", "Sample ID":"Pool_Positive_02", "Factors":{"Treadmill Speed":"Pool"} }, { "Subject ID":"-", "Sample ID":"Pool_Positive_03", "Factors":{"Treadmill Speed":"Pool"} }, { "Subject ID":"-", "Sample ID":"Pool_Positive_04", "Factors":{"Treadmill Speed":"Pool"} }, { "Subject ID":"-", "Sample ID":"Pool_Positive_05", "Factors":{"Treadmill Speed":"Pool"} }, { "Subject ID":"-", "Sample ID":"Subject_#13_Negative_HILIC_01", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#13_Negative_HILIC_02", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#14_Negative_HILIC_01", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#14_Negative_HILIC_02", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#15_Negative_HILIC_01", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#15_Negative_HILIC_02", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#16_Negative_HILIC_01", "Factors":{"Treadmill Speed":"Hard"} }, { "Subject ID":"-", "Sample ID":"Subject_#16_Negative_HILIC_02", "Factors":{"Treadmill Speed":"Hard"} }, { "Subject ID":"-", "Sample ID":"Subject_#17_Negative_HILIC_01", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#17_Negative_HILIC_02", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#19_Negative_HILIC_01", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#19_Negative_HILIC_02", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#20_Negative_HILIC_01", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#20_Negative_HILIC_02", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#21_Negative_HILIC_01", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#21_Negative_HILIC_02", "Factors":{"Treadmill Speed":"Low"} }, { "Subject ID":"-", "Sample ID":"Subject_#23_Negative_HILIC_01", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#23_Negative_HILIC_02", "Factors":{"Treadmill Speed":"Medium"} }, { "Subject ID":"-", "Sample ID":"Subject_#24_Negative_HILIC_01", "Factors":{"Treadmill Speed":"Hard"} }, { "Subject ID":"-", "Sample ID":"Subject_#24_Negative_HILIC_02", "Factors":{"Treadmill Speed":"Hard"} }, { "Subject ID":"-", "Sample ID":"Pool_Negative_01", "Factors":{"Treadmill Speed":"Pool"} }, { "Subject ID":"-", "Sample ID":"Pool_Negative_02", "Factors":{"Treadmill Speed":"Pool"} }, { "Subject ID":"-", "Sample ID":"Pool_Negative_03", "Factors":{"Treadmill Speed":"Pool"} }, { "Subject ID":"-", "Sample ID":"Pool_Negative_04", "Factors":{"Treadmill Speed":"Pool"} }, { "Subject ID":"-", "Sample ID":"Pool_Negative_05", "Factors":{"Treadmill Speed":"Pool"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Sweat collected from the forearms of participants.","SAMPLE_TYPE":"Sweat"}, "TREATMENT":{"TREATMENT_SUMMARY":"Sweat induced by exercise on a treadmill."}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Sweat samples were collected, pooled, and frozen on liquid nitrogen. Following, frozen samples were lyophilized and frozen until analysis."}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"Phenomenex Luna NH2 (100 x 3mm, 3um)"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Thermo LTQ XL","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_RESULTS_FILE":"ST001068_AN001749_Results.txt UNITS:Abundance"} }