#METABOLOMICS WORKBENCH sean_harshman_20181008_114153_mwtab.txt DATATRACK_ID:1534 STUDY_ID:ST001072 ANALYSIS_ID:AN001754 PROJECT_ID:PR000715 VERSION 1 CREATED_ON October 10, 2018, 1:49 pm #PROJECT PR:PROJECT_TITLE The proteomic and metabolomic characterization of exercise-induced sweat PR:PROJECT_SUMMARY Proteomic and Metabolomic analysis of exercise-induced sweat to evaluate analyte PR:PROJECT_SUMMARY correlation with human performance parameters. PR:INSTITUTE UES Inc, Air Force Research Laboratory PR:LAST_NAME Harshman PR:FIRST_NAME Sean PR:ADDRESS 2510 Fifth Street, Area B, Building 840, WPAFB, OH 45433 PR:EMAIL sean.harshman.ctr@us.af.mil PR:PHONE 937-938-3785 #STUDY ST:STUDY_TITLE The in-gel proteomic characterization of exercise-induced sweat - (part III) ST:STUDY_SUMMARY The in-gel proteomic characterization of exercise-induced sweat to add depth to ST:STUDY_SUMMARY the evaluation of the sweat protein content. ST:INSTITUTE UES Inc, Air Force Research Laboratory ST:LAST_NAME Harshman ST:FIRST_NAME Sean ST:ADDRESS 2510 Fifth Street, Area B, Building 840, WPAFB, OH 45433 ST:EMAIL sean.harshman.ctr@us.af.mil ST:PHONE 937-938-3785 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - InGel_#1 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_#2 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_#3 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_#4 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_#5 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_#6 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_#7 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_#8 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_#9 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_#10 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_#11 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_#12 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_#13 Gel:175ug_Peptide SUBJECT_SAMPLE_FACTORS - InGel_A Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_B Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_C Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_D Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_E Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_F Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_G Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_H Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_I Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_J Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_K Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_L Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_M Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_N Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_P Gel:2ug_Protein SUBJECT_SAMPLE_FACTORS - InGel_S Gel:2ug_Protein #COLLECTION CO:COLLECTION_SUMMARY Sweat collected from the forearms of participants. CO:SAMPLE_TYPE Sweat #TREATMENT TR:TREATMENT_SUMMARY Sweat induced by exercise on a treadmill. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Sweat samples were collected, pooled, and frozen on liquid nitrogen. Following, SP:SAMPLEPREP_SUMMARY frozen samples were lyophilized and frozen until analysis. Samples were run in 2 SP:SAMPLEPREP_SUMMARY SDS-PAGE gels, excised, digested, and analyzed by LS-MS-MS. Gel 1 was loaded SP:SAMPLEPREP_SUMMARY based on 175ug of sweat peptides (Factor) and Gel 2 was loaded based on 2ug of SP:SAMPLEPREP_SUMMARY protein (Factor). #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 RS CH:COLUMN_NAME Easy-Spray PepMap 50µm x 150µm, 100Å, 2µm #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Thermo LTQ XL MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Spectral Counts MS_METABOLITE_DATA_START Samples InGel_#1 InGel_#2 InGel_#3 InGel_#4 InGel_#5 InGel_#6 InGel_#7 InGel_#8 InGel_#9 InGel_#10 InGel_#11 InGel_#12 InGel_#13 InGel_A InGel_B InGel_C InGel_D InGel_E InGel_F InGel_G InGel_H InGel_I InGel_J InGel_K InGel_L InGel_M InGel_N InGel_P InGel_S Factors Gel:175ug_Peptide Gel:175ug_Peptide Gel:175ug_Peptide Gel:175ug_Peptide Gel:175ug_Peptide Gel:175ug_Peptide Gel:175ug_Peptide Gel:175ug_Peptide Gel:175ug_Peptide Gel:175ug_Peptide Gel:175ug_Peptide Gel:175ug_Peptide Gel:175ug_Peptide Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Gel:2ug_Protein Keratin; type I cytoskeletal 10 1 105 2 4 5 18 48 3 4 90 2 1 1 3 152 Serum albumin 139 12 4 26 482 7 519 Keratin; type II cytoskeletal 1 15 27 1 1 61 11 41 3 28 56 2 3 133 Prolactin-inducible protein 50 87 25 1 15 2 3 1 9 8 127 308 145 78 130 15 11 4 841 Keratin; type II cytoskeletal 2 epidermal 9 22 1 2 5 42 17 16 3 8 27 1 1 1 57 Zinc-alpha-2-glycoprotein 54 22 7 1 2 14 304 29 17 4 2 2 3 4 3 6 398 Dermcidin 1 3 4 8 10 34 71 15 20 13 6 6 8 43 53 65 63 62 51 56 98 97 105 746 Caspase-14 1 13 6 54 30 3 45 26 158 Carboxypeptidase E 13 1 130 3 1 134 Keratin; type I cytoskeletal 14 11 2 3 1 7 3 14 Keratin; type I cytoskeletal 16 11 Clusterin 10 8 14 2 1 16 2 2 45 Keratin; type II cytoskeletal 6B 1 7 2 3 10 4 7 1 1 1 24 Polyubiquitin-C 110 72 2 90 558 3 653 Keratin; type I cytoskeletal 28 12 5 4 4 8 Secretoglobin family 1D member 2 16 15 1 3 32 75 33 143 Keratin; type II cytoskeletal 1b 2 2 6 1 6 3 5 18 32 Keratin; type I cytoskeletal 9 4 7 14 4 28 46 Keratin; type II cytoskeletal 6C 4 1 5 1 4 1 1 1 13 Beta-2-microglobulin 7 1 30 2 1 34 Arginase-1 5 Thioredoxin 4 1 31 21 1 53 Serpin B7 5 4 13 13 Ig gamma-1 chain C 5 31 2 4 1 38 Cystatin-A 4 1 2 39 39 1 81 Desmoglein-1 2 14 22 11 3 7 2 13 8 5 87 Serpin B3 61 3 9 73 Serotransferrin 65 65 sp|O75223|GGCT_HUMAN Gamma-glutamylcyclotransferase 4 55 59 Fibromodulin 4 23 28 4 59 Protein-glutamine gamma-glutamyltransferase E 28 2 19 2 3 54 Pro-cathepsin 30 20 50 Ig kappa chain C region 15 31 1 47 Serpin B4 28 3 9 40 Mucin-like protein 1 8 24 32 Cathepsin L2 22 3 25 Golgi membrane protein 1 12 9 3 24 Protein S100-A7 16 7 23 Secreted Ly-6/uPAR-related protein 1 7 16 23 Ig lambda-3 chain C regions 13 9 22 Superoxide dismutase [Cu-Zn] 21 21 Catalase 20 20 Alpha-1-antitrypsin 1 18 19 Serpin B12 2 2 3 4 4 4 19 Attractin 17 17 Hemoglobin subunit beta 3 12 2 17 Cathepsin D 16 16 Lysozyme C 4 11 15 Ig gamma-4 chain C 3 11 1 15 Serpin A12 13 13 Alpha-2-macroglobulin 13 13 Cystatin-M 1 2 10 13 N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase 1 10 11 Ig gamma-2 chain C 3 7 1 11 Triosephosphate isomerase 10 10 Ly6/PLAUR domain-containing protein 3 6 3 9 Protein deglycase DJ-1 9 9 Keratin; type II cytoskeletal 5 5 1 3 9 Phosphatidylethanolamine-binding protein 1 8 8 Neuroserpin 8 8 Serpin B8 8 8 Actin; cytoplasmic 1 2 6 8 Cathepsin B 4 4 8 Beta-hexosaminidase subunit beta 1 3 4 8 Keratin; type I cytoskeletal 17 1 1 4 6 Desmocollin-1 1 5 6 Kallikrein-7 5 1 6 Vitamin D-binding protein 6 6 Interleukin-36 gamma 2 4 6 Protein S100-A8 3 3 6 Eukaryotic translation initiation factor 6 2 3 5 Galectin-3-binding protein 4 4 Ribonuclease UK114 4 4 Glutaredoxin-1 4 4 Apolipoprotein A-I 4 4 Carcinoembryonic antigen-related cell adhesion molecule 5 4 4 Angiotensin-converting enzyme 4 4 Protein S100-A7A 4 4 Heat shock-related 70 kDa protein 2 1 3 4 Gamma-glutamyl hydrolase 1 3 4 Ribosyldihydronicotinamide dehydrogenase [quinone] 4 4 Ribonuclease 7 2 2 4 Retinoid-inducible serine carboxypeptidase 3 3 Fatty acid-binding protein; epidermal 3 3 Phospholipase B-like 1 3 3 Biotinidase 3 3 Lysosome-associated membrane glycoprotein 1 2 1 3 Epididymal secretory protein E1 3 3 Cystatin-SN 3 3 Monocyte differentiation antigen CD14 3 3 Cystatin-S 2 2 Proteasome subunit alpha type-7 2 2 Peroxiredoxin-2 2 2 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Uniprot ID Keratin; type I cytoskeletal 10 P13645 Serum albumin P02768 Keratin; type II cytoskeletal 1 P04264 Prolactin-inducible protein P12273 Keratin; type II cytoskeletal 2 epidermal P35908 Zinc-alpha-2-glycoprotein P25311 Dermcidin P81605 Caspase-14 P31944 Carboxypeptidase E P16870 Keratin; type I cytoskeletal 14 P02533 Keratin; type I cytoskeletal 16 P08779 Clusterin P10909 Keratin; type II cytoskeletal 6B P04259 Polyubiquitin-C P0CG48 Keratin; type I cytoskeletal 28 Q7Z3Y7 Secretoglobin family 1D member 2 O95969 Keratin; type II cytoskeletal 1b Q7Z794 Keratin; type I cytoskeletal 9 P35527 Keratin; type II cytoskeletal 6C P48668 Beta-2-microglobulin P61769 Arginase-1 P05089 Thioredoxin P10599 Serpin B7 O75635 Ig gamma-1 chain C P01857 Cystatin-A P01040 Desmoglein-1 Q02413 Serpin B3 P29508 Serotransferrin P02787 sp|O75223|GGCT_HUMAN Gamma-glutamylcyclotransferase O75223 Fibromodulin Q06828 Protein-glutamine gamma-glutamyltransferase E Q08188 Pro-cathepsin P09668 Ig kappa chain C region P01834 Serpin B4 P48594 Mucin-like protein 1 Q96DR8 Cathepsin L2 O60911 Golgi membrane protein 1 Q8NBJ4 Protein S100-A7 P31151 Secreted Ly-6/uPAR-related protein 1 P55000 Ig lambda-3 chain C regions P0CG06 Superoxide dismutase [Cu-Zn] P00441 Catalase P04040 Alpha-1-antitrypsin P01009 Serpin B12 Q96P63 Attractin O75882 Hemoglobin subunit beta P68871 Cathepsin D P07339 Lysozyme C P61626 Ig gamma-4 chain C P01861 Serpin A12 Q8IW75 Alpha-2-macroglobulin P01023 Cystatin-M Q15828 N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase P20933 Ig gamma-2 chain C P01859 Triosephosphate isomerase P60174 Ly6/PLAUR domain-containing protein 3 O95274 Protein deglycase DJ-1 Q99497 Keratin; type II cytoskeletal 5 P13647 Phosphatidylethanolamine-binding protein 1 P30086 Neuroserpin Q99574 Serpin B8 P50452 Actin; cytoplasmic 1 P60709 Cathepsin B P07858 Beta-hexosaminidase subunit beta P07686 Keratin; type I cytoskeletal 17 Q04695 Desmocollin-1 Q08554 Kallikrein-7 P49862 Vitamin D-binding protein P02774 Interleukin-36 gamma Q9NZH8 Protein S100-A8 P05109 Eukaryotic translation initiation factor 6 P56537 Galectin-3-binding protein Q08380 Ribonuclease UK114 P52758 Glutaredoxin-1 P35754 Apolipoprotein A-I P02647 Carcinoembryonic antigen-related cell adhesion molecule 5 P06731 Angiotensin-converting enzyme P12821 Protein S100-A7A Q86SG5 Heat shock-related 70 kDa protein 2 P54652 Gamma-glutamyl hydrolase Q92820 Ribosyldihydronicotinamide dehydrogenase [quinone] P16083 Ribonuclease 7 Q9H1E1 Retinoid-inducible serine carboxypeptidase Q9HB40 Fatty acid-binding protein; epidermal Q01469 Phospholipase B-like 1 Q6P4A8 Biotinidase P43251 Lysosome-associated membrane glycoprotein 1 P11279 Epididymal secretory protein E1 P61916 Cystatin-SN P01037 Monocyte differentiation antigen CD14 P08571 Cystatin-S P01036 Proteasome subunit alpha type-7 O14818 Peroxiredoxin-2 P32119 METABOLITES_END #END