{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001172","ANALYSIS_ID":"AN001937","VERSION":"1","CREATED_ON":"April 19, 2019, 1:13 pm"},

"PROJECT":{"PROJECT_TITLE":"Deep Metabolomics of a High-Grade Serous Ovarian Cancer Triple Knockout Mouse Model.","PROJECT_TYPE":"Untargeted metabolomics","PROJECT_SUMMARY":"High-grade serous carcinoma (HGSC) is the most common and deadliest ovarian cancer (OC) type, accounting for 70–80% of OC deaths. This high mortality is largely due to late diagnosis. Early detection is thus crucial to reduce mortality. Yet tumor pathogenesis of HGSC remains poorly understood, making early detection difficult. Faithfully and reliably representing the clinical nature of human HGSC, a recently-developed triple knockout (TKO) mouse model offers a unique opportunity to examine the entire disease spectrum of HGSC. Deep metabolomics study was performed to serum samples collected from these mice to understand the metabolic alternations associated with HGSC development and progression, and provide guidance toward early detection.","INSTITUTE":"Georgia Institute of Technology","DEPARTMENT":"Chemistry","LABORATORY":"Fernández","LAST_NAME":"Huang","FIRST_NAME":"Danning","ADDRESS":"901 Atlantic Dr NE, Atlanta, GA, 30332, USA","EMAIL":"dhuang74@gatech.edu","PHONE":"404-512-7523"},

"STUDY":{"STUDY_TITLE":"Deep Metabolomics of a High-Grade Serous Ovarian Cancer Triple Knockout Mouse Model.","STUDY_TYPE":"Untargeted metabolomics","STUDY_SUMMARY":"Metabolic alternations were investigated by applying Ultra Performance Liquid Chromatography Mass Spectrometry (UPLC-MS) to serum samples collected from triple knockout (TKO) mice at pre-malignant, early, and advanced stages of HGSC. Samples were analyzed with control mice, which have the same genetic background as TKO mice but develop no tumors. To enhance the selectivity for HGSC-specific metabolite markers, a tumor control group was also included. These were uterine tumor (UT) mice that developed uterine tumors, but no HGSC. All samples were analyzed using reverse phase (RP) and hydrophilic interaction liquid chromatography (HILIC) UPLC-MS analysis in positive and negative ion modes.","INSTITUTE":"Georgia Institute of Technology","DEPARTMENT":"Chemistry","LABORATORY":"Fernández","LAST_NAME":"Huang","FIRST_NAME":"Danning","ADDRESS":"901 Atlantic Dr NE","EMAIL":"dhuang74@gatech.edu","PHONE":"4045127523","NUM_GROUPS":"5","TOTAL_SUBJECTS":"84","NUM_FEMALES":"84"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Female"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"1",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"2",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"3",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"4",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"5",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"6",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"7",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"8",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"9",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"10",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"11",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"12",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"13",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"14",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"15",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"16",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"17",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"18",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"19",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"20",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"21",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"22",
"Factors":{"Group":"pre"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"23",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"24",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"25",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"26",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"27",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"28",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"29",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"30",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"31",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"32",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"33",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"34",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"35",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"36",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"37",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"38",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"39",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"40",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"41",
"Factors":{"Group":"ctrl"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"+/+"}
},
{
"Subject ID":"-",
"Sample ID":"42",
"Factors":{"Group":"ET"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"43",
"Factors":{"Group":"ET"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"44",
"Factors":{"Group":"ET"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"45",
"Factors":{"Group":"ET"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"46",
"Factors":{"Group":"ET"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"47",
"Factors":{"Group":"ET"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"48",
"Factors":{"Group":"ET"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"49",
"Factors":{"Group":"ET"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"50",
"Factors":{"Group":"ET"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"51",
"Factors":{"Group":"ET"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"52",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"53",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"54",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"55",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"56",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"57",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"58",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"59",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"60",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"61",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"62",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"63",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"64",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"65",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"66",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"67",
"Factors":{"Group":"LT"},
"Additional sample data":{"p53":"R172H/+","Pten":"fl/fl","Dicer":"fl/fl","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"68",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"69",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"70",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"71",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"72",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"73",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"74",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"75",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"76",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"77",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"78",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"79",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"80",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"81",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"82",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"83",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
},
{
"Subject ID":"-",
"Sample ID":"84",
"Factors":{"Group":"UT"},
"Additional sample data":{"p53":"fl/fl","Pten":"fl/fl","Dicer":"+/+","Amhr2":"Cre/+"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Blood samples were collected via retro-orbital bleeding after anesthesia, from 22 TKO-Pre mice (average age, 2.0 months; age range, 1.3–3.1), 10 TKO-ET mice (4.8 months: 2.5–5.9), 16 TKO-AT mice (6.4 months: 4.3–10.1), 19 TKO-ctrl mice (3.0 months: 3.0–8.2), and 17 UT mice (5.4 months: 3.7–6.8). Blood samples were centrifuged at 14,000 rpm for 5 minutes at room temperature, and serum was collected and stored at -80 °C until UPLC-MS analysis.","SAMPLE_TYPE":"Blood (serum)"},

"TREATMENT":{"TREATMENT_SUMMARY":"Triple-mutant (TKO) mice (p53 LSL-R172H/+ Dicer1 flox/flox Pten flox/flox Amhr2 cre/+) were generated by mating p53 LSL-R172H/+ Dicer1 flox/flox Pten flox/flox female with Dicer1 flox/flox Pten flox/flox Amhr2 cre/+ male mice. Serum samples were collected from TKO mice at different stages of tumor progression: from premalignant stage to early stage to advanced stage. Precursor lesions are fallopian tubes at premalignant stage, when no tumors are yet present. TKO mice with precursor (i.e. premalignant stage) lesions in the fallopian tubes are referred to as “TKO-Pre”; TKO-ET (early tumor) are TKO mice with early-stage tumors in the fallopian tube without evidence of metastasis; TKO-AT (advanced-stage tumor) are TKO mice with ovarian and peritoneal metastases accompanied by ascites; and TKO-ctrl are control mice (p53 LSL-R172H/+ Dicer1 flox/flox Pten flox/flox), which have the same genetic background as TKO mice but develop no tumors. All TKO mice developed high-grade serous carcinoma (HGSC, i.e. high-grade serous ovarian cancer)."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Serum samples were thawed on ice and subject to two different sample preparation protocols to profile either non-polar or polar sub-metabolomes. Reverse phase (RP) and hydrophilic interaction liquid chromatography (HILIC) UPLC-MS analysis were used for these different polarity metabolite fractions to obtain complementary information. Methanol (for polar) or iso-propanol (for non-polar) was added to a 50 μL serum sample in a 3:1 ratio to precipitate proteins. Samples were vortex mixed for 10 s and centrifuged at 13,000 rpm for 7 min. Then, 150 μL supernatant was frozen at -80 °C for UPLC-MS analysis. A sample blank was prepared with 50 μL of LC-MS grade water, and a pooled quality control (QC) sample was created by mixing 10 μL aliquot of each serum sample. Both the sample blank and the pooled sample were processed with the same procedure as the murine serum samples. Solvent blanks and sample blanks were analyzed together with murine serum samples."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"Waters Acquity BEH C18 (50 x 2.1mm, 1.7 um)","COLUMN_TEMPERATURE":"55","SOLVENT_A":"water/acetonitrile (40:60 v/v), 10 mM ammonium formate and 0.1% formic acid","SOLVENT_B":"acetonitrile/isopropanol (10:90 v/v), 10 mM ammonium formate and 0.1% formic acid","SAMPLE_INJECTION":"5μL"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive HF hybrid Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Samples were analyzed in positive and negative ion modes for both RP and HILIC chromatography. Spectral features were extracted from the raw data using Compound Discoverer v2.1 software.","CAPILLARY_TEMPERATURE":"275 °C","SPRAY_VOLTAGE":"3.0 kV","MS_RESULTS_FILE":"ST001172_AN001937_Results.txt UNITS:chromatographic peak area Has m/z:Neutral masses Has RT:No RT units:No RT data"}

}