{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001282","ANALYSIS_ID":"AN002126","VERSION":"1","CREATED_ON":"November 25, 2019, 1:08 pm"}, "PROJECT":{"PROJECT_TITLE":"Role of Hi1a in mitochondrial metabolic rewiring in M1 Macrophages","PROJECT_TYPE":"metabolomics","PROJECT_SUMMARY":"Study of Metabolome of WT and Hi1a KO Bone Marrow Derived Macrophages in resting state or activated for 18h with LPS+IFN gamma","INSTITUTE":"National Cancer Institute","LABORATORY":"Laboratory of Cancer Immunometabolism","LAST_NAME":"Palmieri","FIRST_NAME":"Erika M","ADDRESS":"1050 Boyles street, Frederick MD 21702","EMAIL":"erikamariana.palmieri@nih.gov","PHONE":"3018461946","PUBLICATIONS":"Nitric Oxide Orchestrates Metabolic Rewiring in M1 Macrophages by Targeting Aconitase 2 and Pyruvate Dehydrogenase contributors: Erika M. Palmieri, Marieli Gonzalez-Cotto, Walter A. Baseler, Luke C. Davies, Bart Ghesquiere, Nunziata Maio, Christopher M. Rice,Tracey A. Rouault, Teresa Cassel, Richard M. Higashi, Andrew N. Lane, Teresa W.-M. Fan, David A. Wink and Daniel","CONTRIBUTORS":"W. McVicar"}, "STUDY":{"STUDY_TITLE":"Role of Hi1a in mitochondrial metabolic rewiring in M1 Macrophages","STUDY_SUMMARY":"Study of Metabolome of WT and Hi1a KO Bone Marrow Derived Macrophages in resting state or activated for 18h with LPS+IFN gamma","INSTITUTE":"National Cancer Institute","LAST_NAME":"Palmieri","FIRST_NAME":"Erika M","ADDRESS":"1050 Boyles street, Frederick MD 21702","EMAIL":"erikamariana.palmieri@nih.gov","PHONE":"3018461946"}, "SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"EMP_05 KO-CTRL", "Factors":{"Hours":"18","LPS (ng/mL)":"-","IFNgamma ng/mL)":"-"} }, { "Subject ID":"-", "Sample ID":"EMP_06 KO-CTRL", "Factors":{"Hours":"18","LPS (ng/mL)":"-","IFNgamma ng/mL)":"-"} }, { "Subject ID":"-", "Sample ID":"EMP_07 KO-CTRL", "Factors":{"Hours":"18","LPS (ng/mL)":"-","IFNgamma ng/mL)":"-"} }, { "Subject ID":"-", "Sample ID":"EMP_08 KO-CTRL", "Factors":{"Hours":"18","LPS (ng/mL)":"-","IFNgamma ng/mL)":"-"} }, { "Subject ID":"-", "Sample ID":"EMP_01 WT-CTRL", "Factors":{"Hours":"18","LPS (ng/mL)":"-","IFNgamma ng/mL)":"-"} }, { "Subject ID":"-", "Sample ID":"EMP_02 WT-CTRL", "Factors":{"Hours":"18","LPS (ng/mL)":"-","IFNgamma ng/mL)":"-"} }, { "Subject ID":"-", "Sample ID":"EMP_03 WT-CTRL", "Factors":{"Hours":"18","LPS (ng/mL)":"-","IFNgamma ng/mL)":"-"} }, { "Subject ID":"-", "Sample ID":"EMP_04 WT-CTRL", "Factors":{"Hours":"18","LPS (ng/mL)":"-","IFNgamma ng/mL)":"-"} }, { "Subject ID":"-", "Sample ID":"EMP_13 KO-LPSIFN", "Factors":{"Hours":"18","LPS (ng/mL)":"100","IFNgamma ng/mL)":"50"} }, { "Subject ID":"-", "Sample ID":"EMP_14 KO-LPSIFN", "Factors":{"Hours":"18","LPS (ng/mL)":"100","IFNgamma ng/mL)":"50"} }, { "Subject ID":"-", "Sample ID":"EMP_15 KO-LPSIFN", "Factors":{"Hours":"18","LPS (ng/mL)":"100","IFNgamma ng/mL)":"50"} }, { "Subject ID":"-", "Sample ID":"EMP_16 KO-LPSIFN", "Factors":{"Hours":"18","LPS (ng/mL)":"100","IFNgamma ng/mL)":"50"} }, { "Subject ID":"-", "Sample ID":"EMP_09 WT-LPSIFN", "Factors":{"Hours":"18","LPS (ng/mL)":"100","IFNgamma ng/mL)":"50"} }, { "Subject ID":"-", "Sample ID":"EMP_10 WT-LPSIFN", "Factors":{"Hours":"18","LPS (ng/mL)":"100","IFNgamma ng/mL)":"50"} }, { "Subject ID":"-", "Sample ID":"EMP_11 WT-LPSIFN", "Factors":{"Hours":"18","LPS (ng/mL)":"100","IFNgamma ng/mL)":"50"} }, { "Subject ID":"-", "Sample ID":"EMP_12 WT-LPSIFN", "Factors":{"Hours":"18","LPS (ng/mL)":"100","IFNgamma ng/mL)":"50"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"WT and Hif1a-/- BMDM (~107 cells per sample) were treated with vehicle (PBS) or 100ng/mL of LPS + 50ng/mL for 18 hours. Cells were then washed, gently scraped, pelleted, snap frozen in liquid nitrogen and sent to West Coast Metabolomic Center at the University of California, Davis (Davis, CA) for metabolomic analyses.","SAMPLE_TYPE":"Macrophages"}, "TREATMENT":{"TREATMENT_SUMMARY":"As mentioned in the sample information."}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"WT and Hif1a-/- BMDM (~107 cells per sample) were treated with vehicle (PBS) or 100ng/mL of LPS + 50ng/mL for 18 hours. Cells were then washed, gently scraped, pelleted, snap frozen in liquid nitrogen and sent to West Coast Metabolomic Center at the University of California, Davis (Davis, CA) for metabolomic analyses."}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 6890N","COLUMN_NAME":"Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"INSTRUMENT_NAME":"Leco Pegasus III GC TOF","INSTRUMENT_TYPE":"GC-TOF","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"Raw GC-TOF MS data files were preprocessed directly after data acquisition and stored as ChromaTOF-specific peg files, as generic txt result files and additionally as generic ANDI MS cdf files. ChromaTOF version 4.0 was used for data preprocessing without smoothing, 3 s peak width, baseline subtraction just above the noise level, and automatic mass spectral deconvolution and peak detection at signal/noise (s/n) levels of 5:1 throughout the chromatogram. Results in .txt format were exported to a data server with absolute spectra intensities and further processed by a filtering algorithm implemented in the metabolomics BinBase database. The BinBase algorithm (rtx5) used the following settings: validity of chromatogram (107 counts/s), unbiased retention index marker detection (MS similarity > 800, validity of intensity range for high m/z"}, "MS_METABOLITE_DATA":{ "Units":"normalized peak height", "Data":[{"Metabolite":"zymosterol","EMP_05 KO-CTRL":"6826","EMP_06 KO-CTRL":"7796","EMP_07 KO-CTRL":"7461","EMP_08 KO-CTRL":"7876","EMP_01 WT-CTRL":"6952","EMP_02 WT-CTRL":"8145","EMP_03 WT-CTRL":"8024","EMP_04 WT-CTRL":"7041","EMP_13 KO-LPSIFN":"6050","EMP_14 KO-LPSIFN":"6717","EMP_15 KO-LPSIFN":"7389","EMP_16 KO-LPSIFN":"5759","EMP_09 WT-LPSIFN":"4909","EMP_10 WT-LPSIFN":"8010","EMP_11 WT-LPSIFN":"5595","EMP_12 WT-LPSIFN":"4372"},{"Metabolite":"xylose","EMP_05 KO-CTRL":"167","EMP_06 KO-CTRL":"152","EMP_07 KO-CTRL":"167","EMP_08 KO-CTRL":"107","EMP_01 WT-CTRL":"243","EMP_02 WT-CTRL":"163","EMP_03 WT-CTRL":"226","EMP_04 WT-CTRL":"170","EMP_13 KO-LPSIFN":"89","EMP_14 KO-LPSIFN":"160","EMP_15 KO-LPSIFN":"139","EMP_16 KO-LPSIFN":"136","EMP_09 WT-LPSIFN":"120","EMP_10 WT-LPSIFN":"82","EMP_11 WT-LPSIFN":"71","EMP_12 WT-LPSIFN":"170"},{"Metabolite":"xylitol","EMP_05 KO-CTRL":"419","EMP_06 KO-CTRL":"331","EMP_07 KO-CTRL":"375","EMP_08 KO-CTRL":"490","EMP_01 WT-CTRL":"351","EMP_02 WT-CTRL":"332","EMP_03 WT-CTRL":"520","EMP_04 WT-CTRL":"504","EMP_13 KO-LPSIFN":"275","EMP_14 KO-LPSIFN":"338","EMP_15 KO-LPSIFN":"292","EMP_16 KO-LPSIFN":"268","EMP_09 WT-LPSIFN":"389","EMP_10 WT-LPSIFN":"394","EMP_11 WT-LPSIFN":"502","EMP_12 WT-LPSIFN":"449"},{"Metabolite":"xanthine","EMP_05 KO-CTRL":"223","EMP_06 KO-CTRL":"250","EMP_07 KO-CTRL":"235","EMP_08 KO-CTRL":"370","EMP_01 WT-CTRL":"322","EMP_02 WT-CTRL":"216","EMP_03 WT-CTRL":"318","EMP_04 WT-CTRL":"336","EMP_13 KO-LPSIFN":"400","EMP_14 KO-LPSIFN":"478","EMP_15 KO-LPSIFN":"764","EMP_16 KO-LPSIFN":"548","EMP_09 WT-LPSIFN":"450","EMP_10 WT-LPSIFN":"726","EMP_11 WT-LPSIFN":"591","EMP_12 WT-LPSIFN":"349"},{"Metabolite":"valine","EMP_05 KO-CTRL":"21274","EMP_06 KO-CTRL":"21347","EMP_07 KO-CTRL":"24088","EMP_08 KO-CTRL":"19716","EMP_01 WT-CTRL":"13914","EMP_02 WT-CTRL":"17566","EMP_03 WT-CTRL":"17402","EMP_04 WT-CTRL":"18621","EMP_13 KO-LPSIFN":"19773","EMP_14 KO-LPSIFN":"21240","EMP_15 KO-LPSIFN":"25169","EMP_16 KO-LPSIFN":"22055","EMP_09 WT-LPSIFN":"25782","EMP_10 WT-LPSIFN":"26599","EMP_11 WT-LPSIFN":"23559","EMP_12 WT-LPSIFN":"23049"},{"Metabolite":"uridine-5-monophosphate","EMP_05 KO-CTRL":"384","EMP_06 KO-CTRL":"176","EMP_07 KO-CTRL":"673","EMP_08 KO-CTRL":"209","EMP_01 WT-CTRL":"327","EMP_02 WT-CTRL":"496","EMP_03 WT-CTRL":"592","EMP_04 WT-CTRL":"412","EMP_13 KO-LPSIFN":"1797","EMP_14 KO-LPSIFN":"248","EMP_15 KO-LPSIFN":"390","EMP_16 KO-LPSIFN":"271","EMP_09 WT-LPSIFN":"418","EMP_10 WT-LPSIFN":"1040","EMP_11 WT-LPSIFN":"437","EMP_12 WT-LPSIFN":"265"},{"Metabolite":"uridine","EMP_05 KO-CTRL":"592","EMP_06 KO-CTRL":"558","EMP_07 KO-CTRL":"543","EMP_08 KO-CTRL":"684","EMP_01 WT-CTRL":"319","EMP_02 WT-CTRL":"325","EMP_03 WT-CTRL":"614","EMP_04 WT-CTRL":"492","EMP_13 KO-LPSIFN":"377","EMP_14 KO-LPSIFN":"481","EMP_15 KO-LPSIFN":"1059","EMP_16 KO-LPSIFN":"334","EMP_09 WT-LPSIFN":"373","EMP_10 WT-LPSIFN":"1303","EMP_11 WT-LPSIFN":"628","EMP_12 WT-LPSIFN":"360"},{"Metabolite":"uric acid","EMP_05 KO-CTRL":"1414","EMP_06 KO-CTRL":"1203","EMP_07 KO-CTRL":"1807","EMP_08 KO-CTRL":"2082","EMP_01 WT-CTRL":"689","EMP_02 WT-CTRL":"984","EMP_03 WT-CTRL":"1602","EMP_04 WT-CTRL":"1064","EMP_13 KO-LPSIFN":"281","EMP_14 KO-LPSIFN":"233","EMP_15 KO-LPSIFN":"401","EMP_16 KO-LPSIFN":"326","EMP_09 WT-LPSIFN":"465","EMP_10 WT-LPSIFN":"834","EMP_11 WT-LPSIFN":"623","EMP_12 WT-LPSIFN":"373"},{"Metabolite":"urea","EMP_05 KO-CTRL":"7159","EMP_06 KO-CTRL":"4862","EMP_07 KO-CTRL":"3434","EMP_08 KO-CTRL":"4020","EMP_01 WT-CTRL":"5949","EMP_02 WT-CTRL":"4700","EMP_03 WT-CTRL":"4803","EMP_04 WT-CTRL":"6748","EMP_13 KO-LPSIFN":"6114","EMP_14 KO-LPSIFN":"5178","EMP_15 KO-LPSIFN":"3862","EMP_16 KO-LPSIFN":"6903","EMP_09 WT-LPSIFN":"4365","EMP_10 WT-LPSIFN":"4160","EMP_11 WT-LPSIFN":"5653","EMP_12 WT-LPSIFN":"3774"},{"Metabolite":"uracil","EMP_05 KO-CTRL":"871","EMP_06 KO-CTRL":"1013","EMP_07 KO-CTRL":"1301","EMP_08 KO-CTRL":"1872","EMP_01 WT-CTRL":"579","EMP_02 WT-CTRL":"719","EMP_03 WT-CTRL":"706","EMP_04 WT-CTRL":"719","EMP_13 KO-LPSIFN":"355","EMP_14 KO-LPSIFN":"356","EMP_15 KO-LPSIFN":"343","EMP_16 KO-LPSIFN":"330","EMP_09 WT-LPSIFN":"463","EMP_10 WT-LPSIFN":"448","EMP_11 WT-LPSIFN":"591","EMP_12 WT-LPSIFN":"350"},{"Metabolite":"UDP GlcNAc","EMP_05 KO-CTRL":"2084","EMP_06 KO-CTRL":"1725","EMP_07 KO-CTRL":"2605","EMP_08 KO-CTRL":"1748","EMP_01 WT-CTRL":"1416","EMP_02 WT-CTRL":"1962","EMP_03 WT-CTRL":"1627","EMP_04 WT-CTRL":"2138","EMP_13 KO-LPSIFN":"585","EMP_14 KO-LPSIFN":"1229","EMP_15 KO-LPSIFN":"686","EMP_16 KO-LPSIFN":"569","EMP_09 WT-LPSIFN":"1834","EMP_10 WT-LPSIFN":"873","EMP_11 WT-LPSIFN":"977","EMP_12 WT-LPSIFN":"823"},{"Metabolite":"tyrosine","EMP_05 KO-CTRL":"4318","EMP_06 KO-CTRL":"4973","EMP_07 KO-CTRL":"6251","EMP_08 KO-CTRL":"5902","EMP_01 WT-CTRL":"3254","EMP_02 WT-CTRL":"4593","EMP_03 WT-CTRL":"5145","EMP_04 WT-CTRL":"5054","EMP_13 KO-LPSIFN":"4040","EMP_14 KO-LPSIFN":"3647","EMP_15 KO-LPSIFN":"5259","EMP_16 KO-LPSIFN":"5070","EMP_09 WT-LPSIFN":"5321","EMP_10 WT-LPSIFN":"7182","EMP_11 WT-LPSIFN":"8960","EMP_12 WT-LPSIFN":"4790"},{"Metabolite":"tryptophan","EMP_05 KO-CTRL":"1183","EMP_06 KO-CTRL":"1384","EMP_07 KO-CTRL":"2950","EMP_08 KO-CTRL":"1983","EMP_01 WT-CTRL":"1142","EMP_02 WT-CTRL":"1928","EMP_03 WT-CTRL":"1699","EMP_04 WT-CTRL":"1495","EMP_13 KO-LPSIFN":"1343","EMP_14 KO-LPSIFN":"1013","EMP_15 KO-LPSIFN":"1830","EMP_16 KO-LPSIFN":"1320","EMP_09 WT-LPSIFN":"2226","EMP_10 WT-LPSIFN":"2700","EMP_11 WT-LPSIFN":"1038","EMP_12 WT-LPSIFN":"1806"},{"Metabolite":"tocopherol alpha-","EMP_05 KO-CTRL":"342","EMP_06 KO-CTRL":"258","EMP_07 KO-CTRL":"167","EMP_08 KO-CTRL":"314","EMP_01 WT-CTRL":"359","EMP_02 WT-CTRL":"260","EMP_03 WT-CTRL":"312","EMP_04 WT-CTRL":"234","EMP_13 KO-LPSIFN":"478","EMP_14 KO-LPSIFN":"320","EMP_15 KO-LPSIFN":"491","EMP_16 KO-LPSIFN":"405","EMP_09 WT-LPSIFN":"401","EMP_10 WT-LPSIFN":"365","EMP_11 WT-LPSIFN":"379","EMP_12 WT-LPSIFN":"384"},{"Metabolite":"thymine","EMP_05 KO-CTRL":"214","EMP_06 KO-CTRL":"208","EMP_07 KO-CTRL":"154","EMP_08 KO-CTRL":"103","EMP_01 WT-CTRL":"394","EMP_02 WT-CTRL":"160","EMP_03 WT-CTRL":"287","EMP_04 WT-CTRL":"132","EMP_13 KO-LPSIFN":"242","EMP_14 KO-LPSIFN":"359","EMP_15 KO-LPSIFN":"247","EMP_16 KO-LPSIFN":"189","EMP_09 WT-LPSIFN":"221","EMP_10 WT-LPSIFN":"157","EMP_11 WT-LPSIFN":"227","EMP_12 WT-LPSIFN":"184"},{"Metabolite":"thymidine-5-phosphate","EMP_05 KO-CTRL":"395","EMP_06 KO-CTRL":"278","EMP_07 KO-CTRL":"231","EMP_08 KO-CTRL":"229","EMP_01 WT-CTRL":"430","EMP_02 WT-CTRL":"376","EMP_03 WT-CTRL":"639","EMP_04 WT-CTRL":"390","EMP_13 KO-LPSIFN":"222","EMP_14 KO-LPSIFN":"245","EMP_15 KO-LPSIFN":"149","EMP_16 KO-LPSIFN":"252","EMP_09 WT-LPSIFN":"286","EMP_10 WT-LPSIFN":"195","EMP_11 WT-LPSIFN":"313","EMP_12 WT-LPSIFN":"261"},{"Metabolite":"thymidine","EMP_05 KO-CTRL":"504","EMP_06 KO-CTRL":"336","EMP_07 KO-CTRL":"228","EMP_08 KO-CTRL":"354","EMP_01 WT-CTRL":"436","EMP_02 WT-CTRL":"454","EMP_03 WT-CTRL":"539","EMP_04 WT-CTRL":"490","EMP_13 KO-LPSIFN":"352","EMP_14 KO-LPSIFN":"740","EMP_15 KO-LPSIFN":"423","EMP_16 KO-LPSIFN":"462","EMP_09 WT-LPSIFN":"466","EMP_10 WT-LPSIFN":"299","EMP_11 WT-LPSIFN":"635","EMP_12 WT-LPSIFN":"366"},{"Metabolite":"threonine","EMP_05 KO-CTRL":"6576","EMP_06 KO-CTRL":"7047","EMP_07 KO-CTRL":"6582","EMP_08 KO-CTRL":"5608","EMP_01 WT-CTRL":"3674","EMP_02 WT-CTRL":"5960","EMP_03 WT-CTRL":"4963","EMP_04 WT-CTRL":"4586","EMP_13 KO-LPSIFN":"8937","EMP_14 KO-LPSIFN":"8794","EMP_15 KO-LPSIFN":"10893","EMP_16 KO-LPSIFN":"7892","EMP_09 WT-LPSIFN":"12644","EMP_10 WT-LPSIFN":"17243","EMP_11 WT-LPSIFN":"12017","EMP_12 WT-LPSIFN":"12488"},{"Metabolite":"threonic acid","EMP_05 KO-CTRL":"122","EMP_06 KO-CTRL":"131","EMP_07 KO-CTRL":"166","EMP_08 KO-CTRL":"138","EMP_01 WT-CTRL":"160","EMP_02 WT-CTRL":"138","EMP_03 WT-CTRL":"190","EMP_04 WT-CTRL":"142","EMP_13 KO-LPSIFN":"172","EMP_14 KO-LPSIFN":"206","EMP_15 KO-LPSIFN":"132","EMP_16 KO-LPSIFN":"189","EMP_09 WT-LPSIFN":"207","EMP_10 WT-LPSIFN":"138","EMP_11 WT-LPSIFN":"580","EMP_12 WT-LPSIFN":"118"},{"Metabolite":"terephthalic acid","EMP_05 KO-CTRL":"152","EMP_06 KO-CTRL":"173","EMP_07 KO-CTRL":"176","EMP_08 KO-CTRL":"190","EMP_01 WT-CTRL":"291","EMP_02 WT-CTRL":"137","EMP_03 WT-CTRL":"379","EMP_04 WT-CTRL":"542","EMP_13 KO-LPSIFN":"133","EMP_14 KO-LPSIFN":"436","EMP_15 KO-LPSIFN":"213","EMP_16 KO-LPSIFN":"185","EMP_09 WT-LPSIFN":"158","EMP_10 WT-LPSIFN":"165","EMP_11 WT-LPSIFN":"235","EMP_12 WT-LPSIFN":"187"},{"Metabolite":"taurine","EMP_05 KO-CTRL":"1123","EMP_06 KO-CTRL":"3616","EMP_07 KO-CTRL":"35157","EMP_08 KO-CTRL":"3296","EMP_01 WT-CTRL":"15704","EMP_02 WT-CTRL":"12145","EMP_03 WT-CTRL":"100998","EMP_04 WT-CTRL":"5152","EMP_13 KO-LPSIFN":"15125","EMP_14 KO-LPSIFN":"1389","EMP_15 KO-LPSIFN":"2923","EMP_16 KO-LPSIFN":"1696","EMP_09 WT-LPSIFN":"28126","EMP_10 WT-LPSIFN":"27233","EMP_11 WT-LPSIFN":"17432","EMP_12 WT-LPSIFN":"7499"},{"Metabolite":"tartaric acid","EMP_05 KO-CTRL":"106","EMP_06 KO-CTRL":"127","EMP_07 KO-CTRL":"136","EMP_08 KO-CTRL":"79","EMP_01 WT-CTRL":"226","EMP_02 WT-CTRL":"93","EMP_03 WT-CTRL":"92","EMP_04 WT-CTRL":"158","EMP_13 KO-LPSIFN":"120","EMP_14 KO-LPSIFN":"144","EMP_15 KO-LPSIFN":"133","EMP_16 KO-LPSIFN":"161","EMP_09 WT-LPSIFN":"423","EMP_10 WT-LPSIFN":"100","EMP_11 WT-LPSIFN":"65","EMP_12 WT-LPSIFN":"111"},{"Metabolite":"sucrose","EMP_05 KO-CTRL":"75102","EMP_06 KO-CTRL":"59441","EMP_07 KO-CTRL":"73277","EMP_08 KO-CTRL":"63171","EMP_01 WT-CTRL":"75933","EMP_02 WT-CTRL":"117324","EMP_03 WT-CTRL":"117636","EMP_04 WT-CTRL":"134166","EMP_13 KO-LPSIFN":"91060","EMP_14 KO-LPSIFN":"90898","EMP_15 KO-LPSIFN":"111603","EMP_16 KO-LPSIFN":"91442","EMP_09 WT-LPSIFN":"120636","EMP_10 WT-LPSIFN":"184429","EMP_11 WT-LPSIFN":"155218","EMP_12 WT-LPSIFN":"140578"},{"Metabolite":"succinic acid","EMP_05 KO-CTRL":"3648","EMP_06 KO-CTRL":"2090","EMP_07 KO-CTRL":"2074","EMP_08 KO-CTRL":"7058","EMP_01 WT-CTRL":"21595","EMP_02 WT-CTRL":"4410","EMP_03 WT-CTRL":"32352","EMP_04 WT-CTRL":"17143","EMP_13 KO-LPSIFN":"7502","EMP_14 KO-LPSIFN":"40828","EMP_15 KO-LPSIFN":"22967","EMP_16 KO-LPSIFN":"4829","EMP_09 WT-LPSIFN":"23445","EMP_10 WT-LPSIFN":"3040","EMP_11 WT-LPSIFN":"32656","EMP_12 WT-LPSIFN":"4296"},{"Metabolite":"succinate semialdehyde","EMP_05 KO-CTRL":"600","EMP_06 KO-CTRL":"947","EMP_07 KO-CTRL":"984","EMP_08 KO-CTRL":"1455","EMP_01 WT-CTRL":"5943","EMP_02 WT-CTRL":"1166","EMP_03 WT-CTRL":"7841","EMP_04 WT-CTRL":"3733","EMP_13 KO-LPSIFN":"736","EMP_14 KO-LPSIFN":"10133","EMP_15 KO-LPSIFN":"6413","EMP_16 KO-LPSIFN":"683","EMP_09 WT-LPSIFN":"6617","EMP_10 WT-LPSIFN":"847","EMP_11 WT-LPSIFN":"12351","EMP_12 WT-LPSIFN":"470"},{"Metabolite":"stearic acid","EMP_05 KO-CTRL":"114140","EMP_06 KO-CTRL":"71661","EMP_07 KO-CTRL":"82179","EMP_08 KO-CTRL":"85934","EMP_01 WT-CTRL":"123705","EMP_02 WT-CTRL":"89938","EMP_03 WT-CTRL":"103152","EMP_04 WT-CTRL":"115286","EMP_13 KO-LPSIFN":"132025","EMP_14 KO-LPSIFN":"107896","EMP_15 KO-LPSIFN":"131394","EMP_16 KO-LPSIFN":"139571","EMP_09 WT-LPSIFN":"114428","EMP_10 WT-LPSIFN":"103427","EMP_11 WT-LPSIFN":"131867","EMP_12 WT-LPSIFN":"111395"},{"Metabolite":"spermidine","EMP_05 KO-CTRL":"290","EMP_06 KO-CTRL":"373","EMP_07 KO-CTRL":"358","EMP_08 KO-CTRL":"450","EMP_01 WT-CTRL":"977","EMP_02 WT-CTRL":"642","EMP_03 WT-CTRL":"765","EMP_04 WT-CTRL":"723","EMP_13 KO-LPSIFN":"904","EMP_14 KO-LPSIFN":"463","EMP_15 KO-LPSIFN":"364","EMP_16 KO-LPSIFN":"386","EMP_09 WT-LPSIFN":"1236","EMP_10 WT-LPSIFN":"4105","EMP_11 WT-LPSIFN":"716","EMP_12 WT-LPSIFN":"708"},{"Metabolite":"sorbitol","EMP_05 KO-CTRL":"2635","EMP_06 KO-CTRL":"2143","EMP_07 KO-CTRL":"2117","EMP_08 KO-CTRL":"1601","EMP_01 WT-CTRL":"1906","EMP_02 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