{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001617","ANALYSIS_ID":"AN002652","VERSION":"1","CREATED_ON":"December 3, 2020, 11:16 am"},

"PROJECT":{"PROJECT_TITLE":"Unique metabolomic profile of skeletal muscle in chronic limb threatening ischemia","PROJECT_SUMMARY":"This project is focused on a cross-sectional analysis of non-PAD controls and CLTI patients undergoing either a vascular intervention or undergoing limb amputation was performed and involved a detailed assessment of the limb muscle metabolome using HR-MAS and solution state NMR spectroscopy. It was hypothesized that patients undergoing limb amputation would present with altered muscle metaolite features compared with non-PAD controls.","INSTITUTE":"University of Florida","DEPARTMENT":"Applied Physiology and Kinesiology","LABORATORY":"Rm 42 and Rm 43","LAST_NAME":"Ryan","FIRST_NAME":"Terence","ADDRESS":"1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA","EMAIL":"ryant@ufl.edu","PHONE":"352-294-1700","FUNDING_SOURCE":"National Institutes of Health and the National Heart, Lung, and Blood, Institute numbers R01-HL149704 (to T.E.R.) and R01-HL148597 (to S.T.S.); as well as the American Heart Association grant number 18CDA34110044 (to T.E.R.). A portion of this work was supported by pilot funds (awarded to T.E.R.) from The University of Florida Claude D. Pepper Older Americans Independence Center P30AG028740. (if applicable)","PROJECT_COMMENTS":"PAD metabolomic study via NMR. A detailed assessment of the limb muscle metabolome using semi-solid and solution state NMR spectroscopy.","PUBLICATIONS":"Journal of Clinical Medicine","CONTRIBUTORS":"Ram B. Khattri, Kyoungrae Kim, Trace Thome, Zachary R. Salyers, Kerri A. O’Malley, Scott A. Berceli, Salvatore T. Scali, Terence E. Ryan*"},

"STUDY":{"STUDY_TITLE":"Unique metabolomic profile of skeletal muscle in chronic limb threatening ischemia (HR-MAS study)","STUDY_SUMMARY":"This project is focused on a cross-sectional analysis of non-PAD controls and CLTI patients undergoing either a vascular intervention or undergoing limb amputation was performed and involved a detailed assessment of the limb muscle metabolome using HR-MAS and solution state NMR spectroscopy. It was hypothesized that patients undergoing limb amputation would present with altered muscle metaolite features compared with non-PAD controls.","INSTITUTE":"University of Florida","DEPARTMENT":"Applied Physiology and Kinesiology","LABORATORY":"Rm 42 and Rm 43","LAST_NAME":"Ryan","FIRST_NAME":"Terence","ADDRESS":"1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA","EMAIL":"ryant@ufl.edu","PHONE":"352-294-1700","NUM_GROUPS":"3","TOTAL_SUBJECTS":"30","NUM_MALES":"25","NUM_FEMALES":"5","STUDY_COMMENTS":"PAD metabolomic study via NMR. A detailed assessment of the limb muscle metabolome using semi-solid and solution state NMR spectroscopy.","PUBLICATIONS":"Journal of Clinical Medicine"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","AGE_OR_AGE_RANGE":"55-82 years","GENDER":"Male and female","HUMAN_RACE":"unknown","HUMAN_ETHNICITY":"unknown","HUMAN_MEDICATIONS":"Asprin, ACE inhibitor, statins, cilostazol","HUMAN_SMOKING_STATUS":"Controls = 4, CLTI Pre-surgery = 7, and CLTI Amputation = 9"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"CLTI Amputation1B",
"Factors":{"Group":"CLTI_Amputation"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Amputation1B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Amputation2B",
"Factors":{"Group":"CLTI_Amputation"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Amputation2B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Amputation3B",
"Factors":{"Group":"CLTI_Amputation"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Amputation3B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Amputation4B",
"Factors":{"Group":"CLTI_Amputation"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Amputation4B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Amputation6B",
"Factors":{"Group":"CLTI_Amputation"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Amputation6B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Amputation7B",
"Factors":{"Group":"CLTI_Amputation"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Amputation7B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Amputation8B",
"Factors":{"Group":"CLTI_Amputation"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Amputation8B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Amputation9B",
"Factors":{"Group":"CLTI_Amputation"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Amputation9B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Amputation10B",
"Factors":{"Group":"CLTI_Amputation"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Amputation10B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Pre-surgery1B",
"Factors":{"Group":"CLTI_Pre_surgery"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Pre-surgery1B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Pre-surgery2B",
"Factors":{"Group":"CLTI_Pre_surgery"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Pre-surgery2B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Pre-surgery5B",
"Factors":{"Group":"CLTI_Pre_surgery"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Pre-surgery5B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Pre-surgery6B",
"Factors":{"Group":"CLTI_Pre_surgery"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Pre-surgery6B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Pre-surgery7B",
"Factors":{"Group":"CLTI_Pre_surgery"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Pre-surgery7B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"CLTI Pre-surgery9B",
"Factors":{"Group":"CLTI_Pre_surgery"},
"Additional sample data":{"RAW_FILE_NAME":"CLTI Pre-surgery9B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Control1B",
"Factors":{"Group":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"Control1B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Control2B",
"Factors":{"Group":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"Control2B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Control3B",
"Factors":{"Group":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"Control3B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Control4B",
"Factors":{"Group":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"Control4B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Control5B",
"Factors":{"Group":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"Control5B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Control6B",
"Factors":{"Group":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"Control6B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Control7B",
"Factors":{"Group":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"Control7B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Control8B",
"Factors":{"Group":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"Control8B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Control9B",
"Factors":{"Group":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"Control9B.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Control10B",
"Factors":{"Group":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"Control10B.raw"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Gastrocnemius muscle specimens were obtained from ten older adult non-PAD controls (Control), ten patients with critical limb ischemia (CLI) undergoing surgical intervention (CLTI Pre-surgery), and ten CLI patients undergoing limb amputation (CLTI Amputation). Five pre-surgery patients underwent bypass interventions and five underwent endovascular procedures. Muscle specimens were collected within the confines of the operating rooms (CLI patients) or via percutaneous muscle biopsy using sterile procedures. A portion of the muscle was quickly trimmed of fat/connective tissue and snap frozen in liquid nitrogen for metabolomics analysis.","SAMPLE_TYPE":"Muscle","COLLECTION_METHOD":"Muscle specimens were collected within the confines of the operating rooms (CLI patients) or via percutaneous muscle biopsy using sterile procedures. A portion of the muscle was quickly trimmed of fat/connective tissue and snap frozen in liquid nitrogen for metabolomics analysis.","STORAGE_CONDITIONS":"-80℃","STORAGE_VIALS":"cryovials"},

"TREATMENT":{"TREATMENT_SUMMARY":"This was a prospective cohort study that examined the metabolomic profile of skeletal muscle from CLTI patients undergoing surgerical intervention or amputation, as well as a cohort of non-PAD controls. CLTI patients exhibited severe symptomology (Rutherford Classification 3-6), with high incidence of common PAD risk factors including hypertension, hyperlipidemia, coronary artery disease, and diabetes.Ten patients with critical limb ischemia (CLI) undergoing surgical intervention (CLTI Pre-surgery), and ten CLI patients undergoing limb amputation (CLTI Amputation). Five pre-surgery patients underwent bypass interventions and five underwent endovascular procedures. Muscle specimens were collected within the confines of the operating rooms (CLI patients) or via percutaneous muscle biopsy using sterile procedures.","HUMAN_FASTING":"non-fasted","HUMAN_ENDP_CLINICAL_SIGNS":"N/A"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Semi-solid HR-MAS spectra were collected on intact human gastrocnemius muscle specimens.","PROCESSING_METHOD":"None","PROCESSING_STORAGE_CONDITIONS":"-80℃","EXTRACT_STORAGE":"-80℃","SAMPLE_RESUSPENSION":"None","SAMPLE_SPIKING":"None"},

"ANALYSIS":{"DATA_FORMAT":"fid, 1r"},

"NM":{"INSTRUMENT_NAME":"Bruker Avance 800 MHz with HR-MAS console","INSTRUMENT_TYPE":"FT-NMR","NMR_EXPERIMENT_TYPE":"1D-1H","FIELD_FREQUENCY_LOCK":"Deuterium","STANDARD_CONCENTRATION":"None","SPECTROMETER_FREQUENCY":"800 MHz","NMR_PROBE":"HR-MAS probe","NMR_SOLVENT":"For HR-MAS experiments, deuterium oxide was used.","NMR_TUBE_SIZE":"3.2 mm inside diameter plastic insert was used for HR-MAS experiments.","SHIMMING_METHOD":"Topshim","PULSE_SEQUENCE":"noesypr1d","WATER_SUPPRESSION":"presat","PULSE_WIDTH":"90-degree","RECEIVER_GAIN":"28.5","OFFSET_FREQUENCY":"4.96 Hz","CHEMICAL_SHIFT_REF_CPD":"None","TEMPERATURE":"4 oC","NUMBER_OF_SCANS":"256","DUMMY_SCANS":"8","ACQUISITION_TIME":"2.0447 s","RELAXATION_DELAY":"2 s","SPECTRAL_WIDTH":"8012.8 Hz","NUM_DATA_POINTS_ACQUIRED":"16384 points","REAL_DATA_POINTS":"65536","LINE_BROADENING":"0.5 Hz","ZERO_FILLING":"65,536 points","APODIZATION":"Exponential","BASELINE_CORRECTION_METHOD":"Spline","CHEMICAL_SHIFT_REF_STD":"Alanine doublet at 1.46 ppm"},

"NMR_METABOLITE_DATA":{
"Units":"A.U.",

"Data":[{"Metabolite":"Caprate","CLTI Amputation1B":"1551.435009","CLTI Amputation2B":"19272.77922","CLTI Amputation3B":"19765.96697","CLTI Amputation4B":"20886.53302","CLTI Amputation6B":"26545.51978","CLTI Amputation7B":"21320.25958","CLTI Amputation8B":"21728.43875","CLTI Amputation9B":"9761.603044","CLTI Amputation10B":"10487.68556","CLTI Pre-surgery1B":"20321.59869","CLTI Pre-surgery2B":"9923.434213","CLTI Pre-surgery5B":"11824.88871","CLTI Pre-surgery6B":"17680.5786","CLTI Pre-surgery7B":"1169.686947","CLTI Pre-surgery9B":"5850.051607","Control1B":"8631.300572","Control2B":"16351.01911","Control3B":"18203.3365","Control4B":"7063.66292","Control5B":"26200.27514","Control6B":"12003.13494","Control7B":"6607.499913","Control8B":"14691.30089","Control9B":"13485.21917","Control10B":"7605.969989"},{"Metabolite":"CH3-lipid","CLTI Amputation1B":"224125.3283","CLTI Amputation2B":"1005208.575","CLTI Amputation3B":"937843.0114","CLTI Amputation4B":"551717.8773","CLTI Amputation6B":"1543304.908","CLTI Amputation7B":"547227.193","CLTI Amputation8B":"671286.7011","CLTI Amputation9B":"307424.9219","CLTI Amputation10B":"1628391.18","CLTI Pre-surgery1B":"1156141.183","CLTI Pre-surgery2B":"749907.3305","CLTI Pre-surgery5B":"443455.381","CLTI Pre-surgery6B":"1350368.217","CLTI Pre-surgery7B":"380583.1517","CLTI Pre-surgery9B":"510605.3794","Control1B":"732525.8771","Control2B":"199979.2402","Control3B":"516007.2441","Control4B":"333131.3161","Control5B":"625320.0765","Control6B":"662878.0236","Control7B":"172953.286","Control8B":"482728.1686","Control9B":"756392.1776","Control10B":"280960.6525"},{"Metabolite":"2-Aminobutyrate","CLTI Amputation1B":"20638.51377","CLTI Amputation2B":"45894.56372","CLTI Amputation3B":"40513.76504","CLTI Amputation4B":"26734.77663","CLTI Amputation6B":"67556.75625","CLTI Amputation7B":"27502.66641","CLTI Amputation8B":"31220.13401","CLTI Amputation9B":"16473.00353","CLTI Amputation10B":"54517.54998","CLTI Pre-surgery1B":"37451.30314","CLTI Pre-surgery2B":"26449.62752","CLTI Pre-surgery5B":"19678.15971","CLTI Pre-surgery6B":"47451.98572","CLTI Pre-surgery7B":"20703.60896","CLTI Pre-surgery9B":"26393.14217","Control1B":"36061.65118","Control2B":"15620.67776","Control3B":"26038.25866","Control4B":"14574.54804","Control5B":"30781.53386","Control6B":"33029.38286","Control7B":"10404.9706","Control8B":"28441.6048","Control9B":"27595.55734","Control10B":"11253.49135"},{"Metabolite":"(CH2)nlipids+lactate","CLTI Amputation1B":"1124010.746","CLTI Amputation2B":"5233779.925","CLTI Amputation3B":"5106118.908","CLTI Amputation4B":"2698652.314","CLTI Amputation6B":"7963165.474","CLTI Amputation7B":"2813313.212","CLTI Amputation8B":"3549584.876","CLTI Amputation9B":"1466423.887","CLTI Amputation10B":"8289366.699","CLTI Pre-surgery1B":"5894748.264","CLTI Pre-surgery2B":"3671004.287","CLTI Pre-surgery5B":"2241356.882","CLTI Pre-surgery6B":"7452814.529","CLTI Pre-surgery7B":"1950661.143","CLTI Pre-surgery9B":"2428250.189","Control1B":"3835762.003","Control2B":"894146.2406","Control3B":"2654209.539","Control4B":"1826801.741","Control5B":"3129758.225","Control6B":"3456292.832","Control7B":"865590.3316","Control8B":"2620332.513","Control9B":"3767006.24","Control10B":"1364114.407"},{"Metabolite":"Lactate","CLTI Amputation1B":"322054.4574","CLTI Amputation2B":"1018574.867","CLTI Amputation3B":"780749.1139","CLTI Amputation4B":"545692.989","CLTI Amputation6B":"1545031.184","CLTI Amputation7B":"550106.6358","CLTI Amputation8B":"610979.6827","CLTI Amputation9B":"363065.8989","CLTI Amputation10B":"1843197.069","CLTI Pre-surgery1B":"1293599.569","CLTI Pre-surgery2B":"849505.714","CLTI Pre-surgery5B":"458905.5639","CLTI Pre-surgery6B":"1039675.331","CLTI Pre-surgery7B":"374885.5746","CLTI Pre-surgery9B":"614218.6117","Control1B":"634955.9737","Control2B":"150920.5631","Control3B":"427114.7525","Control4B":"266877.0917","Control5B":"603205.4978","Control6B":"670195.0738","Control7B":"131006.9156","Control8B":"389642.2845","Control9B":"818616.5765","Control10B":"276171.0707"},{"Metabolite":"Alanine","CLTI Amputation1B":"11106.83277","CLTI Amputation2B":"7766.663642","CLTI Amputation3B":"6006.755553","CLTI Amputation4B":"1964.852258","CLTI Amputation6B":"4724.001129","CLTI Amputation7B":"5740.832585","CLTI Amputation8B":"7207.774649","CLTI Amputation9B":"2814.444653","CLTI Amputation10B":"15453.3969","CLTI Pre-surgery1B":"1113.82846","CLTI Pre-surgery2B":"1676.116284","CLTI Pre-surgery5B":"2442.282093","CLTI Pre-surgery6B":"12517.7873","CLTI Pre-surgery7B":"10450.89259","CLTI Pre-surgery9B":"7657.988005","Control1B":"9991.766718","Control2B":"82.30495909","Control3B":"2488.091613","Control4B":"6837.054769","Control5B":"1427.97793","Control6B":"11304.39549","Control7B":"2519.425124","Control8B":"8851.586181","Control9B":"2298.096929","Control10B":"1024.384924"},{"Metabolite":"(CH2-CH2-CO-) lipids","CLTI Amputation1B":"102781.908","CLTI Amputation2B":"589303.2171","CLTI Amputation3B":"554757.491","CLTI Amputation4B":"296348.7106","CLTI Amputation6B":"916817.5135","CLTI Amputation7B":"297426.3882","CLTI Amputation8B":"366754.1258","CLTI Amputation9B":"168485.5872","CLTI Amputation10B":"995695.0952","CLTI Pre-surgery1B":"689080.5147","CLTI Pre-surgery2B":"454444.9664","CLTI Pre-surgery5B":"254101.5919","CLTI Pre-surgery6B":"818373.8693","CLTI Pre-surgery7B":"249035.7067","CLTI Pre-surgery9B":"313397.2266","Control1B":"461352.2831","Control2B":"86726.19952","Control3B":"282232.027","Control4B":"188588.9968","Control5B":"340148.3758","Control6B":"405806.6234","Control7B":"87977.36211","Control8B":"279727.0278","Control9B":"445432.3615","Control10B":"152819.1402"},{"Metabolite":"(CH=CH-CH2-CH2) lipids","CLTI Amputation1B":"191173.1486","CLTI Amputation2B":"882466.7402","CLTI Amputation3B":"812810.8706","CLTI Amputation4B":"460510.4251","CLTI Amputation6B":"1522895.203","CLTI Amputation7B":"461719.2533","CLTI Amputation8B":"580796.4152","CLTI Amputation9B":"270461.8235","CLTI Amputation10B":"1565308.842","CLTI Pre-surgery1B":"1083614.964","CLTI Pre-surgery2B":"770822.0649","CLTI Pre-surgery5B":"382667.2242","CLTI Pre-surgery6B":"1193929.974","CLTI Pre-surgery7B":"343876.1457","CLTI Pre-surgery9B":"518986.0222","Control1B":"725698.2539","Control2B":"172937.2663","Control3B":"474514.2474","Control4B":"316846.3877","Control5B":"532082.7176","Control6B":"588329.4084","Control7B":"150379.3165","Control8B":"431651.309","Control9B":"715487.8546","Control10B":"258776.8772"},{"Metabolite":"Glutamine","CLTI Amputation1B":"16878.33137","CLTI Amputation2B":"15191.4304","CLTI Amputation3B":"11016.05033","CLTI Amputation4B":"8859.548684","CLTI Amputation6B":"14105.43814","CLTI Amputation7B":"18990.97623","CLTI Amputation8B":"11286.8191","CLTI Amputation9B":"7265.737983","CLTI Amputation10B":"24605.53281","CLTI Pre-surgery1B":"8965.523716","CLTI Pre-surgery2B":"6033.6823","CLTI Pre-surgery5B":"3856.102619","CLTI Pre-surgery6B":"13102.50017","CLTI Pre-surgery7B":"5275.191684","CLTI Pre-surgery9B":"6500.640413","Control1B":"19337.75126","Control2B":"12865.46104","Control3B":"9041.348125","Control4B":"18309.66414","Control5B":"8596.810231","Control6B":"15936.41499","Control7B":"8726.436345","Control8B":"21039.30098","Control9B":"6096.090031","Control10B":"2606.9394"},{"Metabolite":"O-Cresol?","CLTI Amputation1B":"148.5219721","CLTI Amputation2B":"7903.277893","CLTI Amputation3B":"6940.005401","CLTI Amputation4B":"4373.243989","CLTI Amputation6B":"7865.724835","CLTI Amputation7B":"5604.82066","CLTI Amputation8B":"4834.093293","CLTI Amputation9B":"2238.403894","CLTI Amputation10B":"8044.985796","CLTI Pre-surgery1B":"8819.89171","CLTI Pre-surgery2B":"4586.801217","CLTI Pre-surgery5B":"3627.231506","CLTI Pre-surgery6B":"17614.33138","CLTI Pre-surgery7B":"1935.420863","CLTI Pre-surgery9B":"3197.510664","Control1B":"10215.83147","Control2B":"3316.05438","Control3B":"47099.92581","Control4B":"5110.167607","Control5B":"6256.159197","Control6B":"9697.94099","Control7B":"15360.71746","Control8B":"7365.807457","Control9B":"4973.489239","Control10B":"2165.826314"},{"Metabolite":"(CH2-CH2-CO) lipids","CLTI Amputation1B":"111074.7813","CLTI Amputation2B":"578029.2466","CLTI Amputation3B":"547193.8805","CLTI Amputation4B":"300443.9491","CLTI Amputation6B":"932621.358","CLTI Amputation7B":"298796.6832","CLTI Amputation8B":"379453.2326","CLTI Amputation9B":"168402.9916","CLTI Amputation10B":"986845.7387","CLTI Pre-surgery1B":"697180.3928","CLTI Pre-surgery2B":"465052.9561","CLTI Pre-surgery5B":"255032.8914","CLTI Pre-surgery6B":"788451.5748","CLTI Pre-surgery7B":"227151.2475","CLTI Pre-surgery9B":"305553.2157","Control1B":"452982.0439","Control2B":"91999.17213","Control3B":"292226.5546","Control4B":"188241.8724","Control5B":"342319.9968","Control6B":"393864.4676","Control7B":"89468.03323","Control8B":"272903.872","Control9B":"452970.1777","Control10B":"156829.8055"},{"Metabolite":"Pyruvate/3-Hydroxyisovalerate","CLTI Amputation1B":"524.4770364","CLTI Amputation2B":"1094.55023","CLTI Amputation3B":"668.6957272","CLTI Amputation4B":"70.56481829","CLTI Amputation6B":"1173.934386","CLTI Amputation7B":"2289.2859","CLTI Amputation8B":"953.9883554","CLTI Amputation9B":"334.3944975","CLTI Amputation10B":"696.8668686","CLTI Pre-surgery1B":"2116.677169","CLTI Pre-surgery2B":"1596.504298","CLTI Pre-surgery5B":"1097.404356","CLTI Pre-surgery6B":"1473.028555","CLTI Pre-surgery7B":"891.9808046","CLTI Pre-surgery9B":"3190.231448","Control1B":"1753.259418","Control2B":"499.4055339","Control3B":"264.4912098","Control4B":"870.4533626","Control5B":"209.9789587","Control6B":"2210.617708","Control7B":"757.8967149","Control8B":"1206.266225","Control9B":"1948.968586","Control10B":"650.9048653"},{"Metabolite":"Lipid Y","CLTI Amputation1B":"47952.31847","CLTI Amputation2B":"172656.591","CLTI Amputation3B":"143500.0541","CLTI Amputation4B":"63055.0987","CLTI Amputation6B":"430561.0537","CLTI Amputation7B":"112914.092","CLTI Amputation8B":"225204.4138","CLTI Amputation9B":"62859.11468","CLTI Amputation10B":"316688.0195","CLTI Pre-surgery1B":"419718.4567","CLTI Pre-surgery2B":"400738.6902","CLTI Pre-surgery5B":"361377.6319","CLTI Pre-surgery6B":"487216.7313","CLTI Pre-surgery7B":"371729.2607","CLTI 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}

}