#METABOLOMICS WORKBENCH epannkuk_20210511_095835 DATATRACK_ID:2635 STUDY_ID:ST001790 ANALYSIS_ID:AN002904 PROJECT_ID:PR001137 VERSION 1 CREATED_ON May 11, 2021, 11:44 am #PROJECT PR:PROJECT_TITLE LDR_mouse_biofluid_metabolomics PR:PROJECT_SUMMARY An important component of ionizing radiation (IR) exposure after a radiological PR:PROJECT_SUMMARY incident may include low-dose rate (LDR) exposures either externally or PR:PROJECT_SUMMARY internally, such as from 137Cs deposition. LDR exposures can have different PR:PROJECT_SUMMARY effects compared to acute high-dose rate exposures from a health and PR:PROJECT_SUMMARY biodosimetry perspective. In this study, a novel irradiation system, VAriable PR:PROJECT_SUMMARY Dose-rate External 137Cs irradiatoR (VADER), was used to expose male and female PR:PROJECT_SUMMARY mice to a variable LDR over a 30-day time span to cumulative doses of 1 (only in PR:PROJECT_SUMMARY males), 2, 2.8, 4.1, 8.8 (only in males), or 9.7 Gy to simulate fall-out type PR:PROJECT_SUMMARY exposures. Urine and serum from mice exposed to an acute dose (~0.8 Gy/min) of PR:PROJECT_SUMMARY x-rays were collected in parallel. Radiation markers were identified by global PR:PROJECT_SUMMARY mass spectrometry based metabolomics and the machine learning algorithm Random PR:PROJECT_SUMMARY Forests. PR:INSTITUTE Georgetown University PR:LAST_NAME Pannkuk PR:FIRST_NAME Evan PR:ADDRESS 3970 Reservoir Rd, NW New Research Building E504 PR:EMAIL elp44@georgetown.edu PR:PHONE 2026875650 #STUDY ST:STUDY_TITLE Effect of external low-dose rate radiation on mouse biofluid metabolomic ST:STUDY_TITLE signatures ST:STUDY_SUMMARY An important component of ionizing radiation (IR) exposure after a radiological ST:STUDY_SUMMARY incident may include low-dose rate (LDR) exposures either externally or ST:STUDY_SUMMARY internally, such as from 137Cs deposition. LDR exposures can have different ST:STUDY_SUMMARY effects compared to acute high-dose rate exposures from a health and ST:STUDY_SUMMARY biodosimetry perspective. In this study, a novel irradiation system, VAriable ST:STUDY_SUMMARY Dose-rate External 137Cs irradiatoR (VADER), was used to expose male and female ST:STUDY_SUMMARY mice to a variable LDR over a 30-day time span to cumulative doses of 1 (only in ST:STUDY_SUMMARY males), 2, 2.8, 4.1, 8.8 (only in males), or 9.7 Gy to simulate fall-out type ST:STUDY_SUMMARY exposures. Urine and serum from mice exposed to an acute dose (~0.8 Gy/min) of ST:STUDY_SUMMARY x-rays were collected in parallel. Radiation markers were identified by global ST:STUDY_SUMMARY mass spectrometry based metabolomics and the machine learning algorithm Random ST:STUDY_SUMMARY Forests. ST:INSTITUTE Georgetown University ST:LAST_NAME Pannkuk ST:FIRST_NAME Evan ST:ADDRESS 3970 Reservoir Rd, NW New Research Building E504 ST:EMAIL elp44@georgetown.edu ST:PHONE 2026875650 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENOTYPE_STRAIN C57BL/6 SU:GENDER Male and female SU:ANIMAL_ANIMAL_SUPPLIER Charles River #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS D3c 9 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_09 SUBJECT_SAMPLE_FACTORS D3c 10 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_10 SUBJECT_SAMPLE_FACTORS D3 11 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_11 SUBJECT_SAMPLE_FACTORS D3c 12 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_12 SUBJECT_SAMPLE_FACTORS D2c 13 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_13 SUBJECT_SAMPLE_FACTORS D2 14 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_14 SUBJECT_SAMPLE_FACTORS D30c 15 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_15 SUBJECT_SAMPLE_FACTORS D1 16 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_16 SUBJECT_SAMPLE_FACTORS D5 18 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_18 SUBJECT_SAMPLE_FACTORS D3 22 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_22 SUBJECT_SAMPLE_FACTORS D2c 23 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_23 SUBJECT_SAMPLE_FACTORS D3c 24 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_24 SUBJECT_SAMPLE_FACTORS D2 25 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_25 SUBJECT_SAMPLE_FACTORS D3 26 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_26 SUBJECT_SAMPLE_FACTORS D3c 27 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_27 SUBJECT_SAMPLE_FACTORS D20 28 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_28 SUBJECT_SAMPLE_FACTORS D30 29 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_29 SUBJECT_SAMPLE_FACTORS D2 30 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_30 SUBJECT_SAMPLE_FACTORS D5 31 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_31 SUBJECT_SAMPLE_FACTORS D20c 35 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_35 SUBJECT_SAMPLE_FACTORS D1 36 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_36 SUBJECT_SAMPLE_FACTORS D5c 37 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_37 SUBJECT_SAMPLE_FACTORS D3c 38 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_38 SUBJECT_SAMPLE_FACTORS D2c 39 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_39 SUBJECT_SAMPLE_FACTORS D1c 40 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_40 SUBJECT_SAMPLE_FACTORS D3c 41 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_41 SUBJECT_SAMPLE_FACTORS D1c 42 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_42 SUBJECT_SAMPLE_FACTORS D3c 43 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_43 SUBJECT_SAMPLE_FACTORS D20c 44 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_44 SUBJECT_SAMPLE_FACTORS D20c 48 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_48 SUBJECT_SAMPLE_FACTORS D20 49 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_49 SUBJECT_SAMPLE_FACTORS D1c 50 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_50 SUBJECT_SAMPLE_FACTORS D5c 51 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_51 SUBJECT_SAMPLE_FACTORS D5 52 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_52 SUBJECT_SAMPLE_FACTORS D20 53 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_53 SUBJECT_SAMPLE_FACTORS D5 54 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_54 SUBJECT_SAMPLE_FACTORS D2 55 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_55 SUBJECT_SAMPLE_FACTORS D5c 56 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_56 SUBJECT_SAMPLE_FACTORS D1c 57 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_57 SUBJECT_SAMPLE_FACTORS D3c 61 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_61 SUBJECT_SAMPLE_FACTORS D2c 62 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_62 SUBJECT_SAMPLE_FACTORS D2c 63 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_63 SUBJECT_SAMPLE_FACTORS D5c 64 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_64 SUBJECT_SAMPLE_FACTORS D5c 65 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_65 SUBJECT_SAMPLE_FACTORS D2c 66 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_66 SUBJECT_SAMPLE_FACTORS D20c 67 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_67 SUBJECT_SAMPLE_FACTORS D1c 68 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_68 SUBJECT_SAMPLE_FACTORS D1 69 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_69 SUBJECT_SAMPLE_FACTORS D3 70 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_70 SUBJECT_SAMPLE_FACTORS D1 74 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_74 SUBJECT_SAMPLE_FACTORS D30c 75 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_75 SUBJECT_SAMPLE_FACTORS D2 76 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_76 SUBJECT_SAMPLE_FACTORS D5 77 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_77 SUBJECT_SAMPLE_FACTORS D5 78 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_78 SUBJECT_SAMPLE_FACTORS D2 79 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_79 SUBJECT_SAMPLE_FACTORS D30 80 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_80 SUBJECT_SAMPLE_FACTORS D1 81 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_81 SUBJECT_SAMPLE_FACTORS D1c 82 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_82 SUBJECT_SAMPLE_FACTORS D1c 86 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_86 SUBJECT_SAMPLE_FACTORS D1 87 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_87 SUBJECT_SAMPLE_FACTORS D20 88 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_88 SUBJECT_SAMPLE_FACTORS D2c 89 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_89 SUBJECT_SAMPLE_FACTORS D2 90 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_90 SUBJECT_SAMPLE_FACTORS D2 91 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_91 SUBJECT_SAMPLE_FACTORS D2 92 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_92 SUBJECT_SAMPLE_FACTORS D5c 93 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_93 SUBJECT_SAMPLE_FACTORS D30c 94 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_94 SUBJECT_SAMPLE_FACTORS D1c 95 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_95 SUBJECT_SAMPLE_FACTORS D3c 99 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_99 SUBJECT_SAMPLE_FACTORS D3 100 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_100 SUBJECT_SAMPLE_FACTORS D1 101 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_101 SUBJECT_SAMPLE_FACTORS D5c 102 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_102 SUBJECT_SAMPLE_FACTORS D2 103 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_103 SUBJECT_SAMPLE_FACTORS D3 104 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_104 SUBJECT_SAMPLE_FACTORS D5 105 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_105 SUBJECT_SAMPLE_FACTORS D1 106 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_106 SUBJECT_SAMPLE_FACTORS D1c 107 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_107 SUBJECT_SAMPLE_FACTORS D30c 108 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_108 SUBJECT_SAMPLE_FACTORS D3c 112 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_112 SUBJECT_SAMPLE_FACTORS D3c 113 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_113 SUBJECT_SAMPLE_FACTORS D2 114 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_114 SUBJECT_SAMPLE_FACTORS D5c 115 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_115 SUBJECT_SAMPLE_FACTORS D2 116 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_116 SUBJECT_SAMPLE_FACTORS D5c 117 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_117 SUBJECT_SAMPLE_FACTORS D5 118 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_118 SUBJECT_SAMPLE_FACTORS D30c 119 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_119 SUBJECT_SAMPLE_FACTORS D5 120 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_120 SUBJECT_SAMPLE_FACTORS D1c 121 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_121 SUBJECT_SAMPLE_FACTORS D5 125 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_125 SUBJECT_SAMPLE_FACTORS D1 126 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_126 SUBJECT_SAMPLE_FACTORS D2c 127 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_127 SUBJECT_SAMPLE_FACTORS D5 128 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_128 SUBJECT_SAMPLE_FACTORS D1 129 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_129 SUBJECT_SAMPLE_FACTORS D3 130 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_130 SUBJECT_SAMPLE_FACTORS D20c 131 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_131 SUBJECT_SAMPLE_FACTORS D5c 132 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_132 SUBJECT_SAMPLE_FACTORS D2c 133 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_133 SUBJECT_SAMPLE_FACTORS D1c 134 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_134 SUBJECT_SAMPLE_FACTORS D2c 138 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_138 SUBJECT_SAMPLE_FACTORS D3c 139 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_139 SUBJECT_SAMPLE_FACTORS D2 140 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_140 SUBJECT_SAMPLE_FACTORS D2c 141 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_141 SUBJECT_SAMPLE_FACTORS D3 142 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_142 SUBJECT_SAMPLE_FACTORS D1 143 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_143 SUBJECT_SAMPLE_FACTORS D3 144 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_144 SUBJECT_SAMPLE_FACTORS D3 145 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_145 SUBJECT_SAMPLE_FACTORS D3c 146 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_146 SUBJECT_SAMPLE_FACTORS D20c 147 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_147 SUBJECT_SAMPLE_FACTORS D3 151 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_151 SUBJECT_SAMPLE_FACTORS D30c 152 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_152 SUBJECT_SAMPLE_FACTORS D2 153 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_153 SUBJECT_SAMPLE_FACTORS D5c 154 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_154 SUBJECT_SAMPLE_FACTORS D5c 155 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_155 SUBJECT_SAMPLE_FACTORS D30 156 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_156 SUBJECT_SAMPLE_FACTORS D5 157 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_157 SUBJECT_SAMPLE_FACTORS D1 158 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_158 SUBJECT_SAMPLE_FACTORS D3 159 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_159 SUBJECT_SAMPLE_FACTORS D3 160 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_160 SUBJECT_SAMPLE_FACTORS D1 164 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_164 SUBJECT_SAMPLE_FACTORS D1c 165 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_165 SUBJECT_SAMPLE_FACTORS D5 166 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_166 SUBJECT_SAMPLE_FACTORS D3c 167 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_167 SUBJECT_SAMPLE_FACTORS D1c 168 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_168 SUBJECT_SAMPLE_FACTORS D3 169 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_169 SUBJECT_SAMPLE_FACTORS D3 170 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_170 SUBJECT_SAMPLE_FACTORS D2 171 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_171 SUBJECT_SAMPLE_FACTORS D3 172 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_172 SUBJECT_SAMPLE_FACTORS D1 173 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_173 SUBJECT_SAMPLE_FACTORS D2 177 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_177 SUBJECT_SAMPLE_FACTORS D3 178 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_178 SUBJECT_SAMPLE_FACTORS D2c 179 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_179 SUBJECT_SAMPLE_FACTORS D5c 180 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_180 SUBJECT_SAMPLE_FACTORS D20 181 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_181 SUBJECT_SAMPLE_FACTORS D1c 182 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_182 SUBJECT_SAMPLE_FACTORS D5 183 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_183 SUBJECT_SAMPLE_FACTORS D2c 184 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_184 SUBJECT_SAMPLE_FACTORS D5c 185 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_185 SUBJECT_SAMPLE_FACTORS D1c 186 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_186 SUBJECT_SAMPLE_FACTORS D5c 190 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_190 SUBJECT_SAMPLE_FACTORS D3c 191 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_191 SUBJECT_SAMPLE_FACTORS D3 192 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_192 SUBJECT_SAMPLE_FACTORS D30 193 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_193 SUBJECT_SAMPLE_FACTORS D2c 194 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_194 SUBJECT_SAMPLE_FACTORS D5c 195 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_195 SUBJECT_SAMPLE_FACTORS D1c 196 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_196 SUBJECT_SAMPLE_FACTORS D5c 197 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_197 SUBJECT_SAMPLE_FACTORS D30 198 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_198 SUBJECT_SAMPLE_FACTORS D5 199 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_199 SUBJECT_SAMPLE_FACTORS D5 203 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_203 SUBJECT_SAMPLE_FACTORS D5c 204 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_204 SUBJECT_SAMPLE_FACTORS D2c 205 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_205 SUBJECT_SAMPLE_FACTORS D20 206 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_206 SUBJECT_SAMPLE_FACTORS D3c 207 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_207 SUBJECT_SAMPLE_FACTORS D1 208 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_208 SUBJECT_SAMPLE_FACTORS D30 209 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_209 SUBJECT_SAMPLE_FACTORS D2c 210 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_210 SUBJECT_SAMPLE_FACTORS D20 211 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_211 SUBJECT_SAMPLE_FACTORS D2 212 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_212 SUBJECT_SAMPLE_FACTORS D2 216 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_216 SUBJECT_SAMPLE_FACTORS D30 217 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_217 SUBJECT_SAMPLE_FACTORS D1c 218 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_218 SUBJECT_SAMPLE_FACTORS D1 219 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_219 SUBJECT_SAMPLE_FACTORS D2 221 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_221 SUBJECT_SAMPLE_FACTORS D5 222 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_222 SUBJECT_SAMPLE_FACTORS D20c 223 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_223 SUBJECT_SAMPLE_FACTORS D5 224 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_224 SUBJECT_SAMPLE_FACTORS D20c 225 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_225 SUBJECT_SAMPLE_FACTORS D30c 229 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_229 SUBJECT_SAMPLE_FACTORS D5c 230 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_230 SUBJECT_SAMPLE_FACTORS D3 231 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_231 SUBJECT_SAMPLE_FACTORS D1c 232 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_232 SUBJECT_SAMPLE_FACTORS D20 233 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_233 SUBJECT_SAMPLE_FACTORS D2c 234 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_234 SUBJECT_SAMPLE_FACTORS D1 237 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_237 SUBJECT_SAMPLE_FACTORS D5c 238 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_238 SUBJECT_SAMPLE_FACTORS D5 242 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_242 SUBJECT_SAMPLE_FACTORS D30 243 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_243 SUBJECT_SAMPLE_FACTORS D2 244 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_244 SUBJECT_SAMPLE_FACTORS D20 245 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_245 SUBJECT_SAMPLE_FACTORS D20 246 Factor:LDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_246 SUBJECT_SAMPLE_FACTORS D2c 247 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_247 SUBJECT_SAMPLE_FACTORS D30 248 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_248 SUBJECT_SAMPLE_FACTORS D1 249 Factor:HDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_249 SUBJECT_SAMPLE_FACTORS D30c 250 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_250 SUBJECT_SAMPLE_FACTORS D2c 251 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_251 SUBJECT_SAMPLE_FACTORS D3 252 Factor:LDR | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_252 SUBJECT_SAMPLE_FACTORS D30c 253 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_253 SUBJECT_SAMPLE_FACTORS D20c 254 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_254 SUBJECT_SAMPLE_FACTORS D1c 255 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_255 SUBJECT_SAMPLE_FACTORS D1 235 Factor:HDR | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_235 SUBJECT_SAMPLE_FACTORS D3c 236 Factor:Control | Factor:post RAW_FILE_NAME=HDRVader_urine_POS_236 SUBJECT_SAMPLE_FACTORS D3c 220 Factor:Control | Factor:pre RAW_FILE_NAME=HDRVader_urine_POS_220 #COLLECTION CO:COLLECTION_SUMMARY Spot urine collected before and after irradiation CO:SAMPLE_TYPE Urine CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY The VADER was designed to deliver controlled dose rates in the range 0.1 – 1 TR:TREATMENT_SUMMARY Gy/day to a cohort of up to 15 mice. The VADER uses ~0.5 Ci of retired 137Cs TR:TREATMENT_SUMMARY brachytherapy seeds that are arranged in two platters placed above and below a TR:TREATMENT_SUMMARY “mouse hotel”. The platters can be placed ~0.5 – 60 cm above and below the TR:TREATMENT_SUMMARY mouse hotel allowing implementation of time-variable dose rates. Offline TR:TREATMENT_SUMMARY dosimetry of the VADER was performed annually using a NIST traceable 10x6-6 TR:TREATMENT_SUMMARY ionization chamber (Radcal Corp., Monrovia, CA). Dose uniformity across the TR:TREATMENT_SUMMARY surface was measured using EBT3 film (Ashland, Covington, KY, USA) and the TR:TREATMENT_SUMMARY variation was 15% across the hotel. A lead and high-density concrete brick TR:TREATMENT_SUMMARY shield ensured minimal radiation doses to occupationally exposed personnel TR:TREATMENT_SUMMARY (operators) inside (< 0.1 mGy/wk) and outside the room (< 0.02 mGy/wk). The TR:TREATMENT_SUMMARY mouse hotel consists of an acrylic box (35 x 35 x 12 cm) allowing housing of ≤ TR:TREATMENT_SUMMARY 15 mice with bedding material and food/water ad libitum. Temperature (20 – TR:TREATMENT_SUMMARY 25°C), humidity (40 – 60%), airflow and lighting were fully controlled to TR:TREATMENT_SUMMARY required animal care standards (temperature/humidity sensor, HWg HTemp, TruePath TR:TREATMENT_SUMMARY Technologies Victor, NY). Environmental controls and monitoring were integrated TR:TREATMENT_SUMMARY into the mouse hotel for easy replacement in case of radiation damage. Mice were TR:TREATMENT_SUMMARY monitored in real time using a 180° fisheye ELP USB camera (Amazon). All animal TR:TREATMENT_SUMMARY experiments were approved by the Columbia University Institutional Animal Care TR:TREATMENT_SUMMARY and Use Committee (IACUC; approved protocol AAQ2410) and were conducted under TR:TREATMENT_SUMMARY all relevant federal and state guidelines. Male and female C57BL/6 mice ( TR:TREATMENT_SUMMARY Charles River Laboratories, Frederick, MD, USA) were irradiated in the VADER in TR:TREATMENT_SUMMARY two randomized batches (15 mice) loaded into mouse hotels (one hotel loaded into TR:TREATMENT_SUMMARY the VADER and one in the same room as a zero-dose control). Five mice per time TR:TREATMENT_SUMMARY point were irradiated in two back-to-back runs (run 1: 2, 3, or 5 d; run 2: 5, TR:TREATMENT_SUMMARY 20, or 30 d) to a total dose of 1 Gy (1 d), 2 Gy (2 d), 2.8 Gy (3 d), 4.1 Gy (5 TR:TREATMENT_SUMMARY d), 8.8 Gy (20 d), and 9.7 Gy (30 d) (Figure S1). Urine and serum were flash TR:TREATMENT_SUMMARY frozen and then stored at -80°C until shipped to Georgetown University Medical TR:TREATMENT_SUMMARY Center for analysis. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Samples were prepared and analyzed as previously described.18, 19 Briefly, serum SP:SAMPLEPREP_SUMMARY (5 μl) was deproteinized (195 μl 66% cold acetonitrile [ACN]) with internal SP:SAMPLEPREP_SUMMARY standards (2 μM debrisoquine [M+H]+ = 176.1188; 30 μM 4-nitrobenzoic acid SP:SAMPLEPREP_SUMMARY [M-H]- = 166.0141), vortexed, incubated on ice (10 min), and centrifuged for 10 SP:SAMPLEPREP_SUMMARY min (max speed, 4 °C). Urine (20 μl) was deproteinized (80 μl 50% cold ACN) SP:SAMPLEPREP_SUMMARY and prepared as above. For urine and serum 1 μl of each sample were combined SP:SAMPLEPREP_SUMMARY for a quality control (QC) sample. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Mobile phases consisted of the following: solvent A (water/0.1% formic acid CH:CHROMATOGRAPHY_SUMMARY [FA]), solvent B (ACN/0.1% FA), solvent C (isopropanol [IPA]/ACN (90:10)/0.1% CH:CHROMATOGRAPHY_SUMMARY FA). The gradient for urine was (solvent A and B) 4.0 min 5% B, 4.0 min 20% B, CH:CHROMATOGRAPHY_SUMMARY 5.1 min 95% B, and 1.9 min 5% B at a flow rate of 0.5 ml/min. The gradient for CH:CHROMATOGRAPHY_SUMMARY serum was (solvent A, B, and C) 4.0 min 98:2 A:B, 4.0 min 40:60 A:B, 1.5 min CH:CHROMATOGRAPHY_SUMMARY 2:98 A:B, 2.0 min 2:98 A:C, 0.5 min 50:50 A:C, and 1.0 min 98:2 A:B at a flow CH:CHROMATOGRAPHY_SUMMARY rate of 0.5 ml/min. CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Waters Acquity BEH C18 (50 x 2.1mm, 1.7 um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Waters Synapt G2 S QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Operating conditions for ESI were: capillary voltage 2.75 kV, cone voltage 30 V, MS:MS_COMMENTS desolvation temperature 500°C, desolvation gas flow 1000 L/Hr. MS:MS_RESULTS_FILE ST001790_AN002904_Results.txt UNITS:peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END