#METABOLOMICS WORKBENCH nozomu_kono_20210602_235749_mwtab.txt DATATRACK_ID:2672 STUDY_ID:ST001818 ANALYSIS_ID:AN002950 PROJECT_ID:000000
VERSION             	1
CREATED_ON             	June 3, 2021, 12:04 am
#PROJECT
PR:PROJECT_TITLE                 	Quantification of PIPs species in biological samples.
PR:PROJECT_SUMMARY               	Phosphoinositides (PIPs) species, differing in phosphorylation at hydroxyls of
PR:PROJECT_SUMMARY               	the inositol head group, play roles in various cellular events. Here we
PR:PROJECT_SUMMARY               	developed a supercritical fluid chromatography-mass spectrometry (SFC-MS) method
PR:PROJECT_SUMMARY               	that allows us to quantify molecular species of all seven PIP regioisomers in
PR:PROJECT_SUMMARY               	culture cells and tissues.
PR:INSTITUTE                     	Grad Sch of Pharmaceut Sci, Univ of Tokyo
PR:LAST_NAME                     	Kono
PR:FIRST_NAME                    	Nozomu
PR:ADDRESS                       	Hongo 7-3-1,, Bunyo-ku, Tokyo, 113-0033, Japan
PR:EMAIL                         	nozomu@mol.f.u-tokyo.ac.jp
PR:PHONE                         	+81-3-5841-4723
#STUDY
ST:STUDY_TITLE                   	Quantification of PIPs species in mouse embryonic fibroblasts (MEFs) during
ST:STUDY_TITLE                   	autophagosome formation.
ST:STUDY_SUMMARY                 	Using a supercritical fluid chromatography-mass spectrometry (SFC-MS)-based
ST:STUDY_SUMMARY                 	methodology, we quantified phosphoinositides (PIPs) species in mouse embryonic
ST:STUDY_SUMMARY                 	fibroblasts (MEFs) from WT or FIP200 KO mice during autophagosome formation.
ST:INSTITUTE                     	Grad Sch of Pharmaceut Sci, Univ of Tokyo
ST:LAST_NAME                     	Kono
ST:FIRST_NAME                    	Nozomu
ST:ADDRESS                       	Hongo 7-3-1,, Bunyo-ku, Tokyo, 113-0033, Japan
ST:EMAIL                         	nozomu@mol.f.u-tokyo.ac.jp
ST:PHONE                         	+81-3-5841-4723
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENDER                        	Not applicable
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	WT_DMEM1	Genotype:WT | Medium:DMEM | Bafilomycin:-	RAW_FILE_NAME=Data190411 FIP KO PIPs-WT_DMEM1.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_DMEM2	Genotype:WT | Medium:DMEM | Bafilomycin:-	RAW_FILE_NAME=Data190411 FIP KO PIPs-WT_DMEM2.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_DMEM3	Genotype:WT | Medium:DMEM | Bafilomycin:-	RAW_FILE_NAME=Data190411 FIP KO PIPs-WT_DMEM3.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_DMEM_Baf1	Genotype:WT | Medium:DMEM | Bafilomycin:+	RAW_FILE_NAME=Data190411 FIP KO PIPs-WT_DMEM_Baf1.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_DMEM_Baf2	Genotype:WT | Medium:DMEM | Bafilomycin:+	RAW_FILE_NAME=Data190411 FIP KO PIPs-WT_DMEM_Baf2.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_DMEM_Baf3	Genotype:WT | Medium:DMEM | Bafilomycin:+	RAW_FILE_NAME=Data190411 FIP KO PIPs-WT_DMEM_Baf3.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_EBSS1	Genotype:WT | Medium:EBSS | Bafilomycin:-	RAW_FILE_NAME=Data190411 FIP KO PIPs-WT_EBSS1.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_EBSS2	Genotype:WT | Medium:EBSS | Bafilomycin:-	RAW_FILE_NAME=Data190411 FIP KO PIPs-WT_EBSS2.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_EBSS3	Genotype:WT | Medium:EBSS | Bafilomycin:-	RAW_FILE_NAME=Data190411 FIP KO PIPs-WT_EBSS3.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_EBSS_Baf1	Genotype:WT | Medium:EBSS | Bafilomycin:+	RAW_FILE_NAME=Data190411 FIP KO PIPs-WT_EBSS_Baf1.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_EBSS_Baf2	Genotype:WT | Medium:EBSS | Bafilomycin:+	RAW_FILE_NAME=Data190411 FIP KO PIPs-WT_EBSS_Baf2.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_EBSS_Baf3	Genotype:WT | Medium:EBSS | Bafilomycin:+	RAW_FILE_NAME=Data190411 FIP KO PIPs-WT_EBSS_Baf3.mzML
SUBJECT_SAMPLE_FACTORS           	-	FIP200_KO_DMEM1	Genotype:FIP200_KO | Medium:DMEM | Bafilomycin:-	RAW_FILE_NAME=Data190411 FIP KO PIPs-KO_DMEM1.mzML
SUBJECT_SAMPLE_FACTORS           	-	FIP200_KO_DMEM2	Genotype:FIP200_KO | Medium:DMEM | Bafilomycin:-	RAW_FILE_NAME=Data190411 FIP KO PIPs-KO_DMEM2.mzML
SUBJECT_SAMPLE_FACTORS           	-	FIP200_KO_DMEM3	Genotype:FIP200_KO | Medium:DMEM | Bafilomycin:-	RAW_FILE_NAME=Data190411 FIP KO PIPs-KO_DMEM3.mzML
SUBJECT_SAMPLE_FACTORS           	-	FIP200_KO_DMEM_Baf1	Genotype:FIP200_KO | Medium:DMEM | Bafilomycin:+	RAW_FILE_NAME=Data190411 FIP KO PIPs-KO_DMEM_Baf1.mzML
SUBJECT_SAMPLE_FACTORS           	-	FIP200_KO_DMEM_Baf2	Genotype:FIP200_KO | Medium:DMEM | Bafilomycin:+	RAW_FILE_NAME=Data190411 FIP KO PIPs-KO_DMEM_Baf2.mzML
SUBJECT_SAMPLE_FACTORS           	-	FIP200_KO_DMEM_Baf3	Genotype:FIP200_KO | Medium:DMEM | Bafilomycin:+	RAW_FILE_NAME=Data190411 FIP KO PIPs-KO_DMEM_Baf3.mzML
SUBJECT_SAMPLE_FACTORS           	-	FIP200_KO_EBSS1	Genotype:FIP200_KO | Medium:EBSS | Bafilomycin:-	RAW_FILE_NAME=Data190411 FIP KO PIPs-KO_EBSS1.mzML
SUBJECT_SAMPLE_FACTORS           	-	FIP200_KO_EBSS2	Genotype:FIP200_KO | Medium:EBSS | Bafilomycin:-	RAW_FILE_NAME=Data190411 FIP KO PIPs-KO_EBSS2.mzML
SUBJECT_SAMPLE_FACTORS           	-	FIP200_KO_EBSS3	Genotype:FIP200_KO | Medium:EBSS | Bafilomycin:-	RAW_FILE_NAME=Data190411 FIP KO PIPs-KO_EBSS3.mzML
SUBJECT_SAMPLE_FACTORS           	-	FIP200_KO_EBSS_Baf1	Genotype:FIP200_KO | Medium:EBSS | Bafilomycin:+	RAW_FILE_NAME=Data190411 FIP KO PIPs-KO_EBSS_Baf1.mzML
SUBJECT_SAMPLE_FACTORS           	-	FIP200_KO_EBSS_Baf2	Genotype:FIP200_KO | Medium:EBSS | Bafilomycin:+	RAW_FILE_NAME=Data190411 FIP KO PIPs-KO_EBSS_Baf2.mzML
SUBJECT_SAMPLE_FACTORS           	-	FIP200_KO_EBSS_Baf3	Genotype:FIP200_KO | Medium:EBSS | Bafilomycin:+	RAW_FILE_NAME=Data190411 FIP KO PIPs-KO_EBSS_Baf3.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	MEFs were maintained in Dulbecco’s modified Eagle’s medium (DMEM;
CO:COLLECTION_SUMMARY            	Sigma-Aldrich) supplemented with 10% fetal calf serum, 100 units/ml penicillin,
CO:COLLECTION_SUMMARY            	100 µg/ml streptomycin, and 2 mM L-glutamine. For autophagy induction, 2 × 105
CO:COLLECTION_SUMMARY            	cells were seeded on 6-well plates and the next day, cells were washed twice
CO:COLLECTION_SUMMARY            	with PBS and incubated in amino acid-free EBSS (starvation medium; Thermo Fisher
CO:COLLECTION_SUMMARY            	Scientific 24010-043) in the presence or absence of bafilomycin A1 (Selleck
CO:COLLECTION_SUMMARY            	Chemicals) for 30 min at 37 ºC. After the incubation, cells were washed twice
CO:COLLECTION_SUMMARY            	with PBS and collected to a safe-lock poly-propylene tube (2 ml) with 1 M HCl
CO:COLLECTION_SUMMARY            	(500 μl), followed by centrifugation (15,000 g, 5 min at 4 ºC). Supernatants
CO:COLLECTION_SUMMARY            	were removed rapidly and resuspended with 750 μl of quench mix, 170 μl of H2O.
CO:SAMPLE_TYPE                   	Fibroblasts
#TREATMENT
TR:TREATMENT_SUMMARY             	MEFs were maintained in Dulbecco’s modified Eagle’s medium (DMEM;
TR:TREATMENT_SUMMARY             	Sigma-Aldrich) supplemented with 10% fetal calf serum, 100 units/ml penicillin,
TR:TREATMENT_SUMMARY             	100 µg/ml streptomycin, and 2 mM L-glutamine. For autophagy induction, 2 × 105
TR:TREATMENT_SUMMARY             	cells were seeded on 6-well plates and the next day, cells were washed twice
TR:TREATMENT_SUMMARY             	with PBS and incubated in amino acid-free EBSS (starvation medium; Thermo Fisher
TR:TREATMENT_SUMMARY             	Scientific 24010-043) in the presence or absence of bafilomycin A1 (Selleck
TR:TREATMENT_SUMMARY             	Chemicals) for 30 min at 37 ºC.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	The following solutions were prepared for lipid extractions; the quench mixture
SP:SAMPLEPREP_SUMMARY            	comprising 484 ml MeOH, 242 ml CHCl3, and 23.55 ml 1 M HCl; the
SP:SAMPLEPREP_SUMMARY            	pre-derivatization wash composed of 240 ml CHCl3, 120 ml MeOH and 90 ml 0.01 M
SP:SAMPLEPREP_SUMMARY            	HCl; and the post-derivatization wash made up of 240 ml CHCl3, 120 ml MeOH, and
SP:SAMPLEPREP_SUMMARY            	90 ml H2O. Wash mixtures were shaken and allowed to separate into two phases.
SP:SAMPLEPREP_SUMMARY            	For pre/post-derivatization wash, the upper phase of each solution was used.
SP:SAMPLEPREP_SUMMARY            	Cells were washed twice with PBS and collected to a safe-lock poly-propylene
SP:SAMPLEPREP_SUMMARY            	tube (2 ml) with 1 M HCl (500 μl), followed by centrifugation (15,000 g, 5 min
SP:SAMPLEPREP_SUMMARY            	at 4 ºC). Supernatants were removed rapidly and resuspended with 750 μl of
SP:SAMPLEPREP_SUMMARY            	quench mix, 170 μl of H2O and internal standards [10 μl containing 2 ng
SP:SAMPLEPREP_SUMMARY            	12:0/13:0 PI, 17:0/20:4 PI (4)P, 17:0/20:4 PI (4,5)P2 and 17:0/20:4 PI
SP:SAMPLEPREP_SUMMARY            	(3,4,5)P3], followed by vortex-mixing and lipid extract steps. Lipid extraction
SP:SAMPLEPREP_SUMMARY            	was performed, based on procedures described by J Clark et al. The single-phase
SP:SAMPLEPREP_SUMMARY            	sample (a mixture of 170 μl of an aqueous sample, 2 ng of internal standards,
SP:SAMPLEPREP_SUMMARY            	and 750 μl of quench mix) were mixed with 725 μl of CHCl3 and 170 μl of 2M
SP:SAMPLEPREP_SUMMARY            	HCl, followed by vortex-mixing and centrifugation (15,000 g, 5 min at room
SP:SAMPLEPREP_SUMMARY            	temperature). The lower organic phase was collected into a fresh safe-lock
SP:SAMPLEPREP_SUMMARY            	poly-propylene tube (2 ml) and mixed with 708 μl of prederivatization wash,
SP:SAMPLEPREP_SUMMARY            	followed by vortex-mixing and centrifugation (15,000 g, 3 min at room
SP:SAMPLEPREP_SUMMARY            	temperature). The lower phase was collected into another fresh tube and
SP:SAMPLEPREP_SUMMARY            	subjected to derivatization. Derivatization of lipids was performed in a fume
SP:SAMPLEPREP_SUMMARY            	hood with adequate personal safety equipment as follows, based on procedures
SP:SAMPLEPREP_SUMMARY            	described by J Clark et al7. Fifty μl trimethylsilyl diazomethane in hexane (2
SP:SAMPLEPREP_SUMMARY            	M solution; Sigma-Aldrich) was added to the lipid extracts (~1 ml), and after
SP:SAMPLEPREP_SUMMARY            	leaving to stand 10 min at room temperature, the reaction was quenched with 6
SP:SAMPLEPREP_SUMMARY            	μl of acetic acid. Next, 700 μl post-derivatization wash solution was added to
SP:SAMPLEPREP_SUMMARY            	the mixture, followed by centrifugation (1,500 g, 3 min). The resultant lower
SP:SAMPLEPREP_SUMMARY            	phase was collected and rewashed with a 700 μl post-derivatization wash
SP:SAMPLEPREP_SUMMARY            	solution. Then 90 μl of MeOH and 10 μl of H2O were added to the final
SP:SAMPLEPREP_SUMMARY            	collected lower phase. The samples were dried up with a centrifugal evaporator.
SP:SAMPLEPREP_SUMMARY            	Finally, samples were dissolved in 80μl MeOH, sonicated briefly, and 20 μl H2O
SP:SAMPLEPREP_SUMMARY            	was added. To avoid degradation, the samples were stored at -80ºC until use.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Other
CH:INSTRUMENT_NAME               	Shimadzu Nexera UC
CH:COLUMN_NAME                   	ULTRON AF-HILIC-CD (250 mm × 2.1 mm, 5.0 µm; Shinwa Chemical Industries)
CH:FLOW_GRADIENT                 	0–16 min: 5% B→20% B; 16.01–18 min: 40% B; 18.01–22 min: 5% B
CH:FLOW_RATE                     	1.5 ml/min
CH:COLUMN_TEMPERATURE            	4℃
CH:SOLVENT_A                     	supercritical carbon dioxide (SCCO2)
CH:SOLVENT_B                     	water/methanol (2.5/97.5, v/v) containing 0.1% (v/v) formic acid
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI SCIEX 4500 QTrap
MS:INSTRUMENT_TYPE               	Ion trap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	The instrument parameters of QTRAP4500 for positive ion mode were as follows:
MS:MS_COMMENTS                   	curtain gas, 20 psi; ionspray voltage, 4500 V; temperature, 300 ºC; ion source
MS:MS_COMMENTS                   	gas 1, 18 psi; ion source gas 2, 20 psi. and quantification was performed using
MS:MS_COMMENTS                   	MultiQuant™ software (SCIEX).
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	PI3P / 36:1PS 
MS_METABOLITE_DATA_START
Samples	WT_DMEM1	WT_DMEM2	WT_DMEM3	WT_DMEM_Baf1	WT_DMEM_Baf2	WT_DMEM_Baf3	WT_EBSS1	WT_EBSS2	WT_EBSS3	WT_EBSS_Baf1	WT_EBSS_Baf2	WT_EBSS_Baf3	FIP200_KO_DMEM1	FIP200_KO_DMEM2	FIP200_KO_DMEM3	FIP200_KO_DMEM_Baf1	FIP200_KO_DMEM_Baf2	FIP200_KO_DMEM_Baf3	FIP200_KO_EBSS1	FIP200_KO_EBSS2	FIP200_KO_EBSS3	FIP200_KO_EBSS_Baf1	FIP200_KO_EBSS_Baf2	FIP200_KO_EBSS_Baf3
Factors	Genotype:WT | Medium:DMEM | Bafilomycin:-	Genotype:WT | Medium:DMEM | Bafilomycin:-	Genotype:WT | Medium:DMEM | Bafilomycin:-	Genotype:WT | Medium:DMEM | Bafilomycin:+	Genotype:WT | Medium:DMEM | Bafilomycin:+	Genotype:WT | Medium:DMEM | Bafilomycin:+	Genotype:WT | Medium:EBSS | Bafilomycin:-	Genotype:WT | Medium:EBSS | Bafilomycin:-	Genotype:WT | Medium:EBSS | Bafilomycin:-	Genotype:WT | Medium:EBSS | Bafilomycin:+	Genotype:WT | Medium:EBSS | Bafilomycin:+	Genotype:WT | Medium:EBSS | Bafilomycin:+	Genotype:FIP200_KO | Medium:DMEM | Bafilomycin:-	Genotype:FIP200_KO | Medium:DMEM | Bafilomycin:-	Genotype:FIP200_KO | Medium:DMEM | Bafilomycin:-	Genotype:FIP200_KO | Medium:DMEM | Bafilomycin:+	Genotype:FIP200_KO | Medium:DMEM | Bafilomycin:+	Genotype:FIP200_KO | Medium:DMEM | Bafilomycin:+	Genotype:FIP200_KO | Medium:EBSS | Bafilomycin:-	Genotype:FIP200_KO | Medium:EBSS | Bafilomycin:-	Genotype:FIP200_KO | Medium:EBSS | Bafilomycin:-	Genotype:FIP200_KO | Medium:EBSS | Bafilomycin:+	Genotype:FIP200_KO | Medium:EBSS | Bafilomycin:+	Genotype:FIP200_KO | Medium:EBSS | Bafilomycin:+
PI3P 32:0	2.24E-06	3.79E-06	3.13668E-06	9.55657E-06	9.93043E-06	6.58481E-06	8.64826E-06	1.19787E-05	2.50446E-05	3.11828E-05	8.47341E-06	2.22222E-05	1.67807E-05	5.24808E-05	5.80769E-06	1.53197E-05	1.55821E-05	-6.89196E-07	7.34364E-06	4.13498E-05	6.20395E-06	4.87909E-06	1.82989E-05	2.20191E-05
PI3P 32:1	1.00368E-05	3.65358E-06	6.35676E-07	8.93731E-06	1.63225E-05	6.22132E-06	1.58188E-05	1.41416E-05	2.52943E-05	2.57378E-05	2.42164E-05	1.24036E-05	1.18719E-05	5.17536E-05	5.91331E-06	1.51279E-05	6.04279E-06	3.89748E-06	2.63614E-05	1.74156E-05	1.41877E-05	7.74133E-06	2.52515E-05	1.34181E-05
PI3P 32:2	5.63091E-06	7.06485E-06	1.12664E-05	1.48242E-05	1.38597E-05	5.78271E-06	1.42078E-05	1.19593E-05	2.80235E-05	3.55754E-05	1.56713E-05	2.19095E-05	3.85405E-05	5.30796E-05	2.07977E-05	1.73529E-05	7.84876E-06	9.30031E-06	1.90958E-05	7.33004E-06	6.86583E-06	8.85332E-06	2.92924E-05	1.0345E-05
PI3P 34:0	6.74027E-06	6.13197E-06	1.28649E-05	9.20489E-06	1.20044E-05	9.24879E-06	9.78198E-06	1.32881E-05	2.72565E-05	3.6216E-05	9.43552E-06	3.3792E-05	1.62923E-05	2.28914E-05	2.4185E-05	1.38875E-05	1.87761E-05	2.0385E-05	1.39925E-05	2.9679E-05	1.19814E-05	3.04856E-06	2.6769E-05	1.13959E-05
PI3P 34:1	2.91377E-05	1.96758E-05	5.63629E-05	4.25917E-05	6.12148E-05	3.47496E-05	2.63445E-05	5.68671E-05	9.45951E-05	0.000157859	7.96975E-05	8.56785E-05	7.69669E-05	0.000175577	7.4267E-05	4.6087E-05	3.54328E-05	4.10728E-05	4.13982E-05	6.18601E-05	3.44242E-05	3.70565E-05	8.03423E-05	6.531E-05
PI3P 34:2	1.42324E-05	1.79067E-05	3.72896E-05	1.40291E-05	3.12976E-05	3.25283E-05	7.37161E-05	3.88167E-05	8.01463E-05	0.000110043	8.83674E-05	8.45112E-05	1.39852E-05	9.01198E-05	6.12457E-05	2.80435E-05	1.10348E-05	2.39321E-05	4.33916E-05	3.10123E-05	1.19286E-05	2.91378E-05	3.13746E-05	1.67409E-05
PI3P 36:0	7.82334E-06	8.17406E-06	7.64479E-06	1.14832E-05	1.90723E-05	8.44911E-06	1.2001E-05	1.12124E-05	2.04067E-05	4.42919E-05	2.93622E-05	1.77715E-05	1.66021E-05	4.30282E-05	3.5458E-05	1.64194E-05	9.28159E-06	1.73462E-05	2.16236E-05	1.60593E-05	7.06957E-06	1.37364E-05	2.2181E-05	1.0194E-05
PI3P 36:1	3.36435E-05	2.5347E-05	5.59614E-05	4.34098E-05	5.53285E-05	5.22859E-05	3.8469E-05	3.85451E-05	5.41206E-05	0.000118943	7.63917E-05	5.37419E-05	1.81718E-05	4.50813E-05	0.000134948	2.29923E-05	3.201E-05	2.55867E-05	3.46382E-05	4.45761E-05	1.42194E-05	1.21011E-05	3.13217E-05	3.56598E-05
PI3P 36:2	4.95268E-05	5.11433E-05	8.94208E-05	5.91284E-05	6.38653E-05	5.93538E-05	4.1703E-05	6.08729E-05	7.08883E-05	0.000248913	0.000158116	0.000152012	2.04295E-05	6.42002E-05	6.50883E-05	2.85806E-05	5.48657E-05	4.11809E-05	4.6685E-05	5.20823E-05	3.81822E-05	3.41625E-05	5.90083E-05	5.6089E-05
PI3P 36:3	2.28549E-05	1.25711E-05	3.23475E-05	1.81651E-05	2.25842E-05	3.28029E-05	1.5734E-05	4.24248E-05	5.37281E-05	8.63647E-05	4.9462E-05	1.38378E-05	3.45116E-05	5.21814E-05	4.09033E-05	2.97698E-05	4.04876E-05	3.26592E-05	4.10082E-05	1.99177E-05	1.09786E-05	1.38454E-05	2.4475E-05	3.3275E-05
PI3P 36:4	1.52787E-05	2.13083E-05	4.77992E-05	2.43731E-05	3.58034E-05	1.87561E-05	4.23934E-05	9.21629E-06	5.41027E-05	7.07962E-05	6.70045E-05	7.75902E-05	1.92132E-05	0.000101754	6.05901E-05	4.2954E-05	2.10448E-05	2.51865E-05	3.77004E-05	3.82881E-05	2.29494E-05	2.54807E-05	2.27281E-05	4.531E-05
PI3P 36:5	6.47739E-06	5.58134E-06	1.46718E-05	8.11927E-06	2.80177E-05	1.67932E-05	2.42006E-05	2.63434E-05	3.6122E-05	4.44063E-05	2.799E-05	1.60163E-05	3.30483E-05	2.48332E-05	3.03041E-05	1.82097E-05	1.26766E-05	1.33665E-05	2.19847E-05	2.83951E-05	1.80513E-05	7.49455E-06	4.16799E-05	1.79809E-05
PI3P 36:6	6.78233E-06	3.32651E-07	8.69498E-06	1.29664E-05	1.28548E-06	6.51292E-06	2.66715E-06	7.64306E-06	2.53657E-05	1.61313E-05	2.01466E-05	2.10131E-05	6.70468E-06	1.26775E-05	1.68712E-05	1.3913E-05	5.23682E-06	5.1779E-06	7.73364E-06	1.27177E-05	2.8766E-06	6.84341E-06	9.33122E-06	1.41924E-05
PI3P 38:2	1.68297E-05	9.08987E-06	2.51583E-05	1.49388E-05	2.15461E-05	8.2391E-06	1.97432E-05	1.25897E-05	3.16447E-05	3.60558E-05	2.68205E-05	1.97102E-05	1.69346E-05	1.96578E-05	1.96503E-05	1.24194E-05	1.51642E-05	1.19174E-05	1.68424E-05	2.39835E-05	1.75235E-05	2.43409E-05	2.29045E-05	7.80922E-06
PI3P 38:3	7.41851E-05	6.05802E-05	0.000117452	5.99312E-05	0.000116289	7.91922E-05	9.68629E-05	9.55965E-05	0.000163432	0.000277282	0.00014232	0.000119095	4.50589E-05	7.81437E-05	8.51393E-05	6.9335E-05	7.67761E-05	6.98172E-05	8.87477E-05	8.74897E-05	7.63961E-05	4.53915E-05	9.16358E-05	8.11606E-05
PI3P 38:4	0.000235068	0.000228953	0.000423089	0.000261544	0.000417007	0.000376898	0.000385296	0.000323569	0.00078309	0.000653558	0.000362545	0.000291849	0.000385025	0.00014521	0.00048971	0.000118913	0.00013393	0.00020969	0.000204102	0.000218601	0.00019033	0.000158375	0.000212987	0.000218283
PI3P 38:5	6.47213E-05	4.9306E-05	0.000116834	5.73165E-05	7.54279E-05	6.71971E-05	5.52204E-05	7.86227E-05	0.000103104	0.000312743	0.000169344	9.54763E-05	0.000202775	5.82549E-05	7.97123E-05	5.80563E-05	7.07164E-05	9.22245E-05	6.1924E-05	0.000132658	0.000105774	7.19524E-05	8.76831E-05	7.22576E-05
PI3P 38:6	1.62093E-05	1.26792E-05	4.34131E-05	2.05505E-05	2.4053E-05	2.45315E-05	2.87427E-05	1.65082E-05	2.91473E-05	2.93983E-05	4.03399E-05	3.20617E-05	5.52071E-05	7.42515E-05	3.7352E-05	2.90153E-05	2.30547E-05	2.85931E-05	2.13202E-05	3.72016E-05	1.60878E-05	1.87116E-05	3.31745E-05	2.7504E-05
PI3P 40:3	1.85068E-06	1.49317E-06	3.461E-06	4.90596E-06	7.1894E-06	4.32633E-06	4.29021E-06	1.13676E-05	1.35016E-05	2.45482E-05	1.34165E-05	7.13779E-06	4.44318E-06	5.88109E-09	1.39774E-05	1.01982E-05	7.27463E-06	5.87434E-06	7.63686E-06	5.86667E-06	1.87269E-06	5.79584E-07	3.81683E-06	1.88871E-06
PI3P 40:4	7.55521E-06	4.25199E-06	2.11274E-05	1.11009E-05	7.9746E-06	8.62682E-06	9.20058E-06	1.03298E-05	1.6188E-05	1.80256E-05	7.23998E-06	9.83114E-06	4.26264E-06	4.93157E-06	2.18722E-05	1.11266E-05	1.03781E-05	6.94279E-06	6.0624E-06	9.36132E-06	1.32587E-05	5.18831E-06	6.92253E-06	1.593E-05
PI3P 40:5	2.68086E-05	1.96075E-05	4.77066E-05	3.07722E-05	4.86692E-05	2.72213E-05	2.9954E-05	3.88652E-05	7.1584E-05	7.25692E-05	3.10931E-05	2.66E-05	4.50209E-05	2.94696E-05	2.88836E-05	1.83887E-05	3.08358E-05	2.12069E-05	3.64437E-05	1.55309E-05	3.40336E-05	1.42517E-05	3.77448E-05	3.18442E-05
PI3P 40:6	1.46215E-05	5.71104E-06	1.94286E-05	1.70183E-05	3.4677E-05	1.34006E-05	2.586E-05	3.08438E-05	4.46486E-05	4.71288E-05	2.77093E-05	3.93996E-05	3.60509E-05	3.55304E-05	4.10308E-05	2.6202E-05	1.91045E-05	1.96064E-05	2.2028E-05	2.48066E-05	1.95925E-05	1.20614E-05	3.13393E-05	2.54213E-05
PI3P 40:7	8.71714E-06	5.67804E-06	1.84865E-05	6.46789E-06	2.46052E-05	1.19386E-05	1.92829E-05	2.10766E-05	2.07456E-05	5.55022E-05	1.49696E-05	2.49739E-05	4.26264E-05	5.33362E-05	3.76981E-05	1.69821E-05	9.63682E-06	2.00606E-05	2.61447E-05	2.14321E-05	1.96559E-05	1.95243E-05	1.36051E-05	2.58506E-05
PI4P 32:0	4.87907E-06	3.33959E-06	0	0	7.98123E-06	9.74152E-07	0	2.25509E-06	0	0	2.13317E-06	0	5.62714E-06	1.91574E-05	1.27481E-06	1.44246E-06	9.27363E-07	1.91957E-05	-3.93471E-06	-2.76148E-05	3.04339E-06	-4.73736E-07	-7.18193E-06	-1.65024E-05
PI4P 32:1	1.16188E-05	7.45677E-06	2.21597E-05	7.79817E-07	1.48161E-05	2.27932E-05	5.25024E-06	1.67119E-06	3.14324E-05	8.09998E-05	1.75113E-06	3.7226E-06	3.53269E-05	5.72113E-05	1.57949E-05	0	2.45443E-05	7.9442E-06	6.97674E-06	2.38683E-05	1.06007E-05	6.95639E-06	1.35352E-06	2.1717E-05
PI4P 32:2	1.4858E-06	3.24289E-06	5.15753E-06	0	3.69067E-06	2.1567E-06	0	0	0	0	0	0	9.90688E-06	8.72387E-06	1.99718E-05	2.09028E-05	2.47861E-06	1.34022E-05	1.09201E-05	1.30255E-05	6.71171E-06	6.72349E-06	0	1.06064E-05
PI4P 34:0	7.67403E-06	3.61149E-06	1.14834E-05	7.29358E-06	8.00221E-06	1.06866E-05	0	0	2.44649E-05	1.07779E-05	1.59426E-05	0	2.68985E-05	4.72027E-05	1.72519E-05	9.93606E-06	1.15801E-05	0	2.51336E-06	0	5.70041E-06	1.29613E-05	0	0
PI4P 34:1	0.000118948	5.59329E-05	8.15753E-05	4.26988E-05	1.02706E-06	6.19144E-05	6.68241E-05	1.23686E-05	6.7071E-05	8.24754E-05	2.49961E-05	0	1.01672E-05	0	5.24859E-05	1.5601E-05	2.76517E-05	1.44804E-05	3.01892E-06	0	1.13692E-05	0	0	0
PI4P 34:2	2.19522E-05	1.24848E-05	4.39537E-05	5.4961E-05	2.64329E-05	2.37076E-05	0	9.95538E-06	0	0	0	0	4.78183E-05	0	-2.42943E-05	0	3.40498E-05	9.11647E-06	0	1.36955E-05	2.25272E-05	0	0	1.38315E-05
PI4P 36:0	7.72871E-06	4.36177E-06	3.10641E-05	4.55352E-06	3.42352E-06	1.84836E-05	1.61047E-05	2.61882E-06	2.38655E-05	0	0	7.68397E-06	1.9713E-05	0	9.34438E-06	1.06777E-06	6.35522E-06	3.56872E-07	0	1.0507E-05	7.71561E-06	-5.74827E-06	0	4.46582E-06
PI4P 36:1	0.00015113	9.71957E-05	0.000201467	0.000159358	0.000176875	0.00015517	0.000177047	0.000137342	0.000288263	0.000301418	0.000116061	0.000120367	0.000246442	0.000174559	0.000192897	4.43095E-05	6.65473E-05	4.63394E-05	8.86754E-05	7.58041E-05	6.55336E-05	3.59366E-05	7.76425E-05	8.79173E-05
PI4P 36:2	0.000194911	0.000159551	0.000415189	0.000268035	0.000262706	0.000276745	0.000246148	0.00024775	0.000568534	0.000449073	0.000246078	0.000164832	0.00034732	0.000307442	0.000369514	0.000119565	0.000194149	0.000115551	0.000170094	0.000225745	0.00017249	9.64817E-05	0.000188495	0.000232242
PI4P 36:3	7.26656E-05	4.65245E-05	0.000112981	8.50497E-05	9.87631E-05	5.14297E-05	0.000112984	4.33075E-05	7.16554E-05	0.00017186	5.63075E-05	9.3375E-05	6.20106E-05	7.24294E-05	0.000111801	2.60614E-05	4.20597E-05	3.01179E-05	3.40329E-05	7.04033E-05	6.4763E-05	2.54014E-05	8.09423E-05	7.73641E-05
PI4P 36:4	8.38801E-05	4.80319E-05	9.19073E-05	6.8805E-05	0.00012476	0.000126171	0.000119151	0.000109814	0.000190653	0.000177625	8.95213E-05	7.39566E-05	9.68814E-05	0	7.96631E-05	2.69949E-05	4.90537E-05	4.52471E-05	-2.26593E-05	0.00010225	6.0247E-05	7.07631E-05	0.000123152	8.90175E-05
PI4P 36:5	5.14353E-06	7.47207E-06	2.15065E-05	5.77217E-06	8.51463E-06	7.78352E-06	1.43362E-05	1.15519E-05	1.45808E-05	2.91535E-05	2.09559E-05	2.5591E-05	2.61764E-05	2.93413E-05	1.90931E-05	1.49193E-05	2.16348E-05	1.86352E-05	1.95324E-05	2.73514E-05	1.82455E-05	1.39623E-05	2.99365E-05	2.1531E-05
PI4P 36:6	5.96104E-06	2.50353E-06	8.37915E-06	8.23043E-06	1.50138E-05	8.37641E-06	2.0384E-05	1.74053E-05	6.04709E-06	1.84695E-05	1.16576E-05	1.3571E-05	1.68643E-05	2.51069E-06	1.91258E-05	3.55499E-06	1.31781E-05	1.81032E-06	1.70475E-05	1.15852E-05	1.65629E-05	0	6.28904E-06	1.04229E-05
PI4P 38:2	6.2755E-05	3.34073E-05	0.000117413	8.50635E-05	5.40585E-05	5.75283E-05	7.16691E-05	7.14355E-05	0.000109811	0.000191672	6.10011E-05	7.3393E-05	9.19783E-05	4.53636E-05	9.3635E-05	2.53977E-05	5.50259E-05	5.07949E-05	4.6516E-05	4.71374E-05	4.12435E-05	1.30426E-05	6.40903E-05	4.15231E-05
PI4P 38:3	0.000446951	0.000285108	0.000907977	0.000348922	0.000510768	0.000426502	0.000483249	0.000367517	0.000970799	0.000989705	0.000500405	0.000369543	0.00052862	0.000492173	0.000535203	0.000118785	0.000269294	0.000236596	0.000325899	0.00043279	0.000343101	0.000341705	0.000478895	0.000270731
PI4P 38:4	0.001008675	0.000702617	0.001945251	0.001042232	0.00156942	0.001219548	0.001377059	0.001064103	0.001917463	0.002343171	0.001661063	0.001447571	0.001353668	0.001515783	0.00169583	0.000568939	0.001121692	0.000748243	0.000968467	0.00126716	0.001002745	0.001313865	0.001507641	0.001361129
PI4P 38:5	0.000172734	0.000139386	0.000285992	0.000208616	0.000252292	0.000232754	0.000230681	7.05917E-05	0.00039629	0.000433768	0.0002678	0.000193142	0.000251805	0.000384688	0.00033167	0.000142584	0.000226189	0.000151617	0.000224469	0.00020935	0.000220923	0.000201189	0.000300053	0.000324367
PI4P 38:6	8.81667E-06	4.719E-06	1.13931E-05	1.79098E-05	1.328E-05	1.27714E-05	1.67829E-05	1.4742E-05	2.6975E-05	4.66804E-05	3.10354E-05	3.27434E-05	2.17807E-05	1.56766E-05	2.6864E-05	2.40831E-05	1.81393E-05	1.73116E-05	1.17738E-05	2.09136E-05	2.04888E-05	1.4337E-05	3.42298E-05	2.55962E-05
PI4P 40:3	2.28044E-05	1.61718E-05	3.50402E-05	1.8185E-05	2.50668E-05	2.5542E-05	2.3641E-05	1.02037E-05	3.81752E-05	4.89499E-05	1.56588E-05	3.62039E-05	4.49905E-05	4.80207E-05	4.13586E-05	8.49105E-06	8.91343E-06	1.25727E-05	2.66647E-06	2.4316E-05	2.55484E-05	1.40539E-05	2.7429E-05	3.01161E-05
PI4P 40:4	3.04348E-05	3.3843E-05	6.01622E-05	3.56881E-05	4.83733E-05	5.10501E-05	5.79118E-05	3.18448E-05	0.00010023	0.000121075	6.18478E-05	5.22764E-05	8.61403E-05	5.09752E-05	8.69969E-05	3.34156E-05	4.83294E-05	4.52244E-05	5.42814E-05	6.72329E-05	5.38351E-05	3.21506E-05	5.73496E-05	6.0814E-05
PI4P 40:5	4.30231E-05	3.11832E-05	8.76834E-05	6.8211E-05	3.36278E-05	7.04523E-05	5.26042E-05	3.61154E-05	6.84088E-05	0.000206559	7.86512E-05	9.13342E-05	0.000104477	6.64927E-05	0.000165784	4.78478E-05	5.49055E-05	7.02163E-05	6.06673E-06	5.72675E-05	1.15697E-05	4.52002E-05	5.71272E-05	7.40763E-05
PI4P 40:6	4.16588E-05	2.71746E-05	9.25019E-05	3.61437E-05	2.43125E-05	3.87221E-05	5.35053E-05	3.15092E-05	7.52765E-05	0.000122995	7.07968E-05	2.66521E-05	6.62961E-05	7.787E-05	7.04516E-05	7.40665E-06	3.88557E-05	3.15216E-05	9.88011E-06	5.27407E-05	4.29684E-05	2.34381E-05	5.43744E-05	4.16932E-05
PI4P 40:7	1.25499E-05	1.15694E-05	4.41853E-05	1.46468E-05	3.02374E-05	1.60695E-05	2.37427E-05	1.65761E-05	4.75437E-05	3.85701E-05	3.247E-05	3.35168E-05	3.31433E-05	5.15312E-05	1.71007E-05	3.34322E-05	1.80438E-05	1.39007E-05	2.49227E-05	2.08609E-05	1.40346E-05	1.87988E-05	2.33916E-05	1.07949E-05
PI5P 32:0	2.44848E-06	2.28612E-06	3.77992E-06	9.56422E-06	4.16455E-06	3.3538E-06	7.75073E-06	1.45975E-06	5.54941E-06	1.69572E-05	6.65835E-07	6.76256E-06	6.51653E-06	2.01925E-05	1.42251E-05	6.42199E-06	4.16716E-06	3.88991E-06	2.04824E-05	6.44609E-06	1.17597E-05	8.20119E-06	4.75737E-06	1.80604E-05
PI5P 32:1	5.10042E-06	4.79408E-06	6.10039E-06	8.44801E-06	7.49199E-06	6.93861E-06	1.10877E-06	1.3065E-06	1.3894E-05	6.78334E-06	9.94386E-06	1.73588E-07	1.50019E-05	3.16253E-05	3.05773E-05	5.6867E-06	1.06468E-05	7.38618E-06	1.57302E-05	2.09053E-05	5.04592E-07	5.84737E-06	-1.71237E-07	5.96184E-06
PI5P 32:2	3.09359E-06	1.95051E-06	1.74595E-06	4.70642E-06	6.27388E-07	1.38207E-06	6.09254E-06	1.96314E-06	2.12451E-06	1.00435E-05	2.42164E-06	6.71461E-06	4.17522E-06	-3.83405E-07	1.21981E-05	8.00256E-06	1.71244E-06	9.33925E-07	4.01271E-06	2.63868E-06	3.20701E-06	3.50942E-06	1.09158E-05	2.45628E-06
PI5P 34:0	1.1062E-06	4.13595E-06	6.17683E-06	6.42202E-06	5.71397E-06	4.05493E-06	9.78198E-06	1.32881E-05	7.0371E-06	8.43972E-06	2.75222E-06	3.3792E-05	1.62923E-05	9.41831E-06	5.49445E-06	1.38875E-05	3.55423E-06	7.39051E-06	2.86437E-06	1.16872E-05	1.19814E-05	3.04856E-06	2.6769E-05	1.13959E-05
PI5P 34:1	1.21136E-05	1.42662E-05	1.17143E-05	1.75382E-05	4.52789E-05	2.458E-05	4.42951E-05	9.3579E-06	7.05851E-05	5.06749E-05	2.891E-05	3.52095E-05	4.40327E-05	4.83319E-05	3.87179E-05	2.24041E-05	1.31244E-05	1.37991E-05	2.69103E-05	2.71276E-05	2.28122E-05	1.45491E-05	1.94989E-05	1.26995E-05
PI5P 34:2	8.08097E-07	2.17122E-07	1.16525E-05	7.30963E-06	7.64771E-06	1.01454E-05	2.41158E-05	7.27837E-06	2.20121E-05	9.14207E-06	1.97412E-05	2.36606E-05	1.74762E-05	3.99145E-05	3.13968E-05	8.19821E-06	5.92836E-06	2.47648E-05	8.55698E-06	1.94239E-05	1.25092E-05	1.69177E-05	5.64673E-06	3.88394E-06
PI5P 36:0	3.6409E-06	3.03299E-06	3.54054E-06	6.48624E-06	1.37714E-05	-3.97981E-07	1.11386E-05	7.9195E-07	1.26846E-05	1.0771E-05	6.6412E-06	4.77173E-06	1.78848E-05	1.01668E-05	1.44764E-05	7.44885E-06	8.71144E-06	1.23824E-05	9.55944E-06	3.78765E-06	3.85728E-06	1.22101E-05	6.34904E-06	7.96343E-06
PI5P 36:1	1.80652E-05	1.45449E-05	6.34981E-05	2.56728E-05	3.59359E-05	2.80856E-05	2.67321E-05	2.40155E-05	4.7913E-05	8.47861E-05	4.22267E-05	2.11799E-05	6.98594E-05	9.7006E-06	6.75287E-05	1.41688E-05	2.93035E-05	3.56764E-05	2.30103E-05	1.12082E-05	2.69714E-05	1.1893E-05	1.83342E-05	1.77742E-05
PI5P 36:2	3.81073E-05	2.43458E-05	7.56988E-05	3.12462E-05	7.17725E-05	3.98304E-05	6.52253E-05	4.56935E-05	0.000112879	7.33242E-05	4.39576E-05	4.94267E-05	5.22425E-05	8.17365E-05	4.69131E-05	2.79412E-05	2.30249E-05	1.94333E-05	1.20237E-05	2.15967E-05	4.19403E-05	3.20912E-05	3.60685E-05	4.55326E-05
PI5P 36:3	9.57939E-06	1.31456E-05	2.87259E-05	6.03593E-06	3.72391E-05	3.70921E-05	1.77204E-05	1.48497E-05	3.23939E-05	2.77511E-05	4.9462E-05	2.74547E-05	3.45116E-05	2.2438E-05	3.48753E-05	2.97698E-05	1.09353E-05	1.1301E-05	1.42554E-05	0.00002	1.14114E-05	1.59068E-05	1.98871E-05	6.05405E-06
PI5P 36:4	6.36172E-06	6.25711E-06	2.09112E-05	1.83869E-05	1.33738E-05	8.38449E-06	3.66885E-06	1.14258E-05	3.76204E-05	3.78403E-05	9.20474E-06	1.63498E-05	9.96009E-06	0.000101754	1.65307E-05	9.66752E-06	3.24478E-06	1.1474E-05	4.6035E-06	1.23671E-05	4.50544E-06	2.27849E-05	1.94989E-05	5.97456E-06
PI5P 36:5	3.66299E-06	2.45791E-07	4.62625E-07	3.45566E-06	9.66317E-06	1.01292E-06	1.02883E-05	2.45393E-06	9.33999E-06	3.15031E-06	5.08498E-06	2.67042E-06	1.01406E-05	2.42258E-05	7.71444E-06	1.82992E-07	1.31045E-06	2.27966E-06	9.35577E-07	3.06831E-06	1.73757E-06	6.57086E-06	9.14946E-06	8.469E-06
PI5P 36:6	3.06046E-07	1.52275E-06	2.05328E-06	5.3104E-07	2.24296E-06	1.24152E-06	4.64995E-06	1.0194E-07	2.53657E-05	5.11553E-06	2.29846E-06	2.10131E-05	6.70468E-06	1.26775E-05	1.68712E-05	1.3913E-05	5.23682E-06	5.1779E-06	7.73364E-06	1.27177E-05	2.8766E-06	6.84341E-06	9.33122E-06	1.41924E-05
PI5P 38:2	1.12671E-05	8.76564E-06	2.18301E-05	6.11391E-06	1.87631E-05	2.06624E-05	2.4782E-05	1.3065E-05	4.58437E-05	3.60558E-05	4.13067E-05	1.95622E-06	2.21779E-05	1.08982E-05	1.5234E-05	9.51023E-06	8.25771E-06	1.02844E-05	1.08725E-05	1.30601E-05	1.55917E-05	1.30723E-05	2.54456E-05	1.70747E-05
PI5P 38:3	7.19243E-05	3.92947E-05	4.87027E-05	2.73089E-05	5.55494E-05	2.78271E-05	4.05281E-05	4.99806E-05	6.6625E-05	5.46786E-05	7.16046E-05	6.27476E-05	3.32193E-05	5.25235E-05	5.14114E-05	8.52813E-05	0.000111542	7.63058E-05	7.57475E-05	6.57284E-05	0.000103251	3.02775E-05	3.46391E-05	7.21781E-05
PI5P 38:4	8.53838E-05	8.94767E-05	0.000148263	5.70795E-05	4.67035E-05	0.000276737	0.000169574	4.58875E-05	0.00013391	0.000289636	7.68127E-05	8.13008E-05	0.000203535	0.000113601	3.62775E-05	6.22634E-05	7.27363E-05	0.000100032	0.000108089	0.000251687	0.000165523	2.42914E-05	0.00010217	0.000132766
PI5P 38:5	2.22187E-05	1.47213E-05	3.339E-05	2.30581E-05	2.0265E-05	1.43457E-05	1.53343E-05	7.86227E-05	4.76989E-05	3.45459E-05	3.36192E-05	2.51199E-05	8.77993E-05	7.03165E-05	5.90239E-05	1.13798E-05	1.58308E-05	1.52698E-05	2.47436E-05	3.82387E-05	2.98955E-05	2.29931E-05	3.02982E-05	7.50874E-06
PI5P 38:6	2.94795E-07	2.91468E-06	5.17066E-06	3.19113E-06	3.73827E-06	6.03312E-06	1.50678E-06	3.871E-06	9.41491E-07	5.87051E-06	7.38656E-06	3.61267E-06	6.70658E-06	2.50128E-06	1.18357E-05	5.32864E-06	3.69154E-06	2.84633E-06	2.65636E-06	4.81481E-06	4.26581E-06	1.58969E-06	4.76795E-06	1.57234E-05
PI5P 40:3	4.26183E-06	1.49317E-06	1.04324E-06	1.83257E-06	5.8995E-06	2.71648E-06	6.06831E-06	1.13676E-05	7.43846E-06	1.42851E-05	1.24341E-05	2.22848E-06	4.44318E-06	5.88109E-09	1.39774E-05	1.01982E-05	7.27463E-06	5.87434E-06	7.63686E-06	5.86667E-06	1.87269E-06	5.79584E-07	3.81683E-06	6.53259E-06
PI5P 40:4	4.60726E-06	2.40557E-06	9.44402E-06	8.04281E-06	8.3788E-06	8.62682E-06	9.547E-06	6.71969E-06	1.04281E-05	1.04759E-05	1.01887E-05	1.41693E-05	1.98594E-05	9.70488E-06	2.18722E-05	3.66752E-06	7.09353E-06	2.9642E-06	6.73696E-06	1.45827E-05	8.08825E-06	5.18831E-06	1.20469E-05	1.07599E-05
PI5P 40:5	1.49211E-05	1.25199E-05	9.70656E-06	1.38609E-05	2.01988E-05	1.00808E-05	2.9954E-05	9.258E-06	3.21441E-05	1.32533E-05	1.14782E-05	1.41067E-05	1.81376E-05	1.47733E-05	1.67693E-05	7.31841E-06	1.28259E-05	8.26322E-06	3.64437E-05	1.55309E-05	3.40336E-05	6.79187E-06	1.45862E-05	9.81876E-06
PI5P 40:6	4.18244E-06	3.81513E-06	8.68726E-06	7.24312E-06	1.01877E-05	7.2391E-06	5.8939E-06	7.73424E-06	3.12701E-05	1.49828E-05	1.06003E-05	9.16198E-06	1.32364E-05	1.56416E-05	7.85831E-06	7.78517E-06	1.3801E-05	1.02758E-05	2.2028E-05	2.48066E-05	3.67993E-06	7.57284E-06	7.19252E-06	5.9539E-06
PI5P 40:7	6.53523E-06	3.24175E-06	8.33205E-06	4.65749E-06	1.13749E-05	7.80775E-06	2.80766E-06	3.44326E-06	3.15198E-06	9.56532E-06	3.23873E-06	3.48551E-06	2.2748E-05	1.16852E-05	1.95229E-05	8.40921E-06	8.11045E-06	5.13248E-06	5.64206E-06	1.2637E-05	6.84577E-06	6.21606E-06	5.75966E-06	1.61208E-05
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Class	Q1 Mass (Da)	Q3 Mass (Da)
PI3P 32:0	PI3P	933.6	551.6
PI3P 32:1	PI3P	931.6	549.6
PI3P 32:2	PI3P	929.6	547.6
PI3P 34:0	PI3P	961.6	579.6
PI3P 34:1	PI3P	959.6	577.6
PI3P 34:2	PI3P	957.6	575.6
PI3P 36:0	PI3P	989.6	607.6
PI3P 36:1	PI3P	987.6	605.6
PI3P 36:2	PI3P	985.6	603.6
PI3P 36:3	PI3P	983.6	601.6
PI3P 36:4	PI3P	981.6	599.6
PI3P 36:5	PI3P	979.6	597.6
PI3P 36:6	PI3P	977.6	595.6
PI3P 38:2	PI3P	1013.6	631.6
PI3P 38:3	PI3P	1011.6	629.6
PI3P 38:4	PI3P	1009.6	627.6
PI3P 38:5	PI3P	1007.6	625.6
PI3P 38:6	PI3P	1005.6	623.6
PI3P 40:3	PI3P	1039.6	657.6
PI3P 40:4	PI3P	1037.6	655.6
PI3P 40:5	PI3P	1035.6	653.6
PI3P 40:6	PI3P	1033.6	651.6
PI3P 40:7	PI3P	1031.6	649.6
PI4P 32:0	PI4P	933.6	551.6
PI4P 32:1	PI4P	931.6	549.6
PI4P 32:2	PI4P	929.6	547.6
PI4P 34:0	PI4P	961.6	579.6
PI4P 34:1	PI4P	959.6	577.6
PI4P 34:2	PI4P	957.6	575.6
PI4P 36:0	PI4P	989.6	607.6
PI4P 36:1	PI4P	987.6	605.6
PI4P 36:2	PI4P	985.6	603.6
PI4P 36:3	PI4P	983.6	601.6
PI4P 36:4	PI4P	981.6	599.6
PI4P 36:5	PI4P	979.6	597.6
PI4P 36:6	PI4P	977.6	595.6
PI4P 38:2	PI4P	1013.6	631.6
PI4P 38:3	PI4P	1011.6	629.6
PI4P 38:4	PI4P	1009.6	627.6
PI4P 38:5	PI4P	1007.6	625.6
PI4P 38:6	PI4P	1005.6	623.6
PI4P 40:3	PI4P	1039.6	657.6
PI4P 40:4	PI4P	1037.6	655.6
PI4P 40:5	PI4P	1035.6	653.6
PI4P 40:6	PI4P	1033.6	651.6
PI4P 40:7	PI4P	1031.6	649.6
PI5P 32:0	PI5P	933.6	551.6
PI5P 32:1	PI5P	931.6	549.6
PI5P 32:2	PI5P	929.6	547.6
PI5P 34:0	PI5P	961.6	579.6
PI5P 34:1	PI5P	959.6	577.6
PI5P 34:2	PI5P	957.6	575.6
PI5P 36:0	PI5P	989.6	607.6
PI5P 36:1	PI5P	987.6	605.6
PI5P 36:2	PI5P	985.6	603.6
PI5P 36:3	PI5P	983.6	601.6
PI5P 36:4	PI5P	981.6	599.6
PI5P 36:5	PI5P	979.6	597.6
PI5P 36:6	PI5P	977.6	595.6
PI5P 38:2	PI5P	1013.6	631.6
PI5P 38:3	PI5P	1011.6	629.6
PI5P 38:4	PI5P	1009.6	627.6
PI5P 38:5	PI5P	1007.6	625.6
PI5P 38:6	PI5P	1005.6	623.6
PI5P 40:3	PI5P	1039.6	657.6
PI5P 40:4	PI5P	1037.6	655.6
PI5P 40:5	PI5P	1035.6	653.6
PI5P 40:6	PI5P	1033.6	651.6
PI5P 40:7	PI5P	1031.6	649.6
METABOLITES_END
#END