{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001943","ANALYSIS_ID":"AN003187","VERSION":"1","CREATED_ON":"September 2, 2021, 4:48 pm"},

"PROJECT":{"PROJECT_TITLE":"Urinary signature of chronic kidney disease in patients with severe obesity by CE-MS","PROJECT_TYPE":"Human nephropathy in CKD obese patients","PROJECT_SUMMARY":"Urine metabolomic characterization of severely obese patients with and without chronic kidney disease (CKD) by CE-MS. Analysis was performed in patients before and after bariatric surgery. In the present studio, samples obtained from CKD patients with severe obesity before bariatric surgery will be referred as OD (obese disease). Samples obtained from CKD patients with severe obesity after bariatric surgery will be referred as ODBS (Obese disease bariatric surgery). Patients with severe obesity without CKD will be referenced as O (obese) and after BS, OBS (obese bariatric surgery). In healthy group, when results refer to the first void urine samples, the acronym will be Healthy1V, and the acronym for urine samples collected at 24-hour will be Healthy24h.","INSTITUTE":"University Rey Juan Carlos","DEPARTMENT":"Basics Science of Health","LABORATORY":"LAFEMEX","LAST_NAME":"Lanzon","FIRST_NAME":"Borja","ADDRESS":"Avenida de Atenas S/N, Alcorcón, Madrid, 28922, Spain","EMAIL":"borja.lanzon@urjc.es","PHONE":"663692554"},

"STUDY":{"STUDY_TITLE":"Urinary signature of chronic kidney disease in patients with severe obesity by CE-MS","STUDY_TYPE":"Human nephropathy in CKD obese patients","STUDY_SUMMARY":"Urine metabolomic characterization of severe obese patients with and without chronic kidney disease (CKD) by CE-MS. Analysis was performed in patients before and after bariatric surgery. In the present studio, samples obtained from CKD patients with severe obesity before bariatric surgery will be referred as OD (obese disease). Samples obtained from CKD patients with severe obesity after bariatric surgery will be referred as ODBS (Obese disease bariatric surgery). Patients with severe obesity without CKD will be referenced as O (obese) and after BS, OBS (obese bariatric surgery). In healthy group, when results refer to the first void urine samples, the acronym will be Healthy1V, and the acronym for urine samples collected at 24-hour will be Healthy24h.","INSTITUTE":"University Rey Juan Carlos","DEPARTMENT":"Basics Science of Health","LABORATORY":"LAFEMEX","LAST_NAME":"Lanzon","FIRST_NAME":"Borja","ADDRESS":"Avenida de Atenas S/N","EMAIL":"borja.lanzon@urjc.es","PHONE":"663692554","NUM_GROUPS":"6","TOTAL_SUBJECTS":"27"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","AGE_OR_AGE_RANGE":"53 ± 15","WEIGHT_OR_WEIGHT_RANGE":"116 ± 25","GENDER":"Male and female","HUMAN_INCLUSION_CRITERIA":"(i) body mass index (BMI)>35kg/m2 plus GFR 30–60ml/min and proteinuria >1 g/24h or GFR>60ml/min and proteinuria >2.5 g/24h despite receiving maximally tolerated doses of renin-angiotensin-aldosterone system (RAAS) blocker and (ii) BMI>40kg/m2 with a GFR>30ml/min and proteinuria >0.5 g/24h despite receiving maximally tolerated doses of RAAS blocker. For the renal function criterion, eGFR was used. The follow-up time was 24 months.","HUMAN_EXCLUSION_CRITERIA":"1) Patients who had participated or were participating in another clinical trial or had taken an experimental drug in the last 28 days. 2) Patients with renal transplantation and/or chronic replacement therapy (hemodialysis and/or peritoneal dialysis). 3) Subjects with poorly controlled blood pressure (SBP > 170 mmHg or DBP > 110 mmHg). 4) Patients with a history of cardiovascular events in the past six months. 5) Patients treated with immunosuppressants. 6) Subjects with a history of renovascular disease, autoimmune diseases, cancer, drug use, or obstructive uropathy. 6) Patients who did not sign the informed consent. 7) Patients who were pregnant or lactating. eGFR was used to establish the renal function criteria. Patients were monitored for 24 months."},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"OD_1",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"OD_1","Acronym explanation":"CKD patients with severe obesity"}
},
{
"Subject ID":"-",
"Sample ID":"OD_2",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"OD_2","Acronym explanation":"CKD patients with severe obesity"}
},
{
"Subject ID":"-",
"Sample ID":"OD_3",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"OD_3","Acronym explanation":"CKD patients with severe obesity"}
},
{
"Subject ID":"-",
"Sample ID":"OD_4",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"OD_4","Acronym explanation":"CKD patients with severe obesity"}
},
{
"Subject ID":"-",
"Sample ID":"OD_5",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"OD_5","Acronym explanation":"CKD patients with severe obesity"}
},
{
"Subject ID":"-",
"Sample ID":"OD_6",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"OD_6","Acronym explanation":"CKD patients with severe obesity"}
},
{
"Subject ID":"-",
"Sample ID":"OD_7",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"OD_7","Acronym explanation":"CKD patients with severe obesity"}
},
{
"Subject ID":"-",
"Sample ID":"OD_8",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"OD_8","Acronym explanation":"CKD patients with severe obesity"}
},
{
"Subject ID":"-",
"Sample ID":"ODBS_1",
"Factors":{"Genotype":"ODBS"},
"Additional sample data":{"RAW_FILE_NAME":"ODBS_1","Acronym explanation":"CKD patients with severe obesity after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"ODBS_2",
"Factors":{"Genotype":"ODBS"},
"Additional sample data":{"RAW_FILE_NAME":"ODBS_2","Acronym explanation":"CKD patients with severe obesity after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"ODBS_3",
"Factors":{"Genotype":"ODBS"},
"Additional sample data":{"RAW_FILE_NAME":"ODBS_3","Acronym explanation":"CKD patients with severe obesity after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"ODBS_4",
"Factors":{"Genotype":"ODBS"},
"Additional sample data":{"RAW_FILE_NAME":"ODBS_4","Acronym explanation":"CKD patients with severe obesity after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"ODBS_5",
"Factors":{"Genotype":"ODBS"},
"Additional sample data":{"RAW_FILE_NAME":"ODBS_5","Acronym explanation":"CKD patients with severe obesity after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"ODBS_6",
"Factors":{"Genotype":"ODBS"},
"Additional sample data":{"RAW_FILE_NAME":"ODBS_6","Acronym explanation":"CKD patients with severe obesity after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"ODBS_7",
"Factors":{"Genotype":"ODBS"},
"Additional sample data":{"RAW_FILE_NAME":"ODBS_7","Acronym explanation":"CKD patients with severe obesity after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"ODBS_8",
"Factors":{"Genotype":"ODBS"},
"Additional sample data":{"RAW_FILE_NAME":"ODBS_8","Acronym explanation":"CKD patients with severe obesity after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"O_1",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"O_1","Acronym explanation":"Patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"O_2",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"O_2","Acronym explanation":"Patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"O_3",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"O_3","Acronym explanation":"Patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"O_4",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"O_4","Acronym explanation":"Patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"O_5",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"O_5","Acronym explanation":"Patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"O_6",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"O_6","Acronym explanation":"Patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"O_7",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"O_7","Acronym explanation":"Patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"O_8",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"O_8","Acronym explanation":"Patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"O_9",
"Factors":{"Genotype":"O"},
"Additional sample data":{"RAW_FILE_NAME":"O_9","Acronym explanation":"Patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"OBS_1",
"Factors":{"Genotype":"OBS"},
"Additional sample data":{"RAW_FILE_NAME":"OBS_1","Acronym explanation":"Patients with severe obesity without CKD after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"OBS_2",
"Factors":{"Genotype":"OBS"},
"Additional sample data":{"RAW_FILE_NAME":"OBS_2","Acronym explanation":"Patients with severe obesity without CKD after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"OBS_3",
"Factors":{"Genotype":"OBS"},
"Additional sample data":{"RAW_FILE_NAME":"OBS_3","Acronym explanation":"Patients with severe obesity without CKD after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"OBS_4",
"Factors":{"Genotype":"OBS"},
"Additional sample data":{"RAW_FILE_NAME":"OBS_4","Acronym explanation":"Patients with severe obesity without CKD after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"OBS_5",
"Factors":{"Genotype":"OBS"},
"Additional sample data":{"RAW_FILE_NAME":"OBS_5","Acronym explanation":"Patients with severe obesity without CKD after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"OBS_6",
"Factors":{"Genotype":"OBS"},
"Additional sample data":{"RAW_FILE_NAME":"OBS_6","Acronym explanation":"Patients with severe obesity without CKD after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"OBS_7",
"Factors":{"Genotype":"OBS"},
"Additional sample data":{"RAW_FILE_NAME":"OBS_7","Acronym explanation":"Patients with severe obesity without CKD after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"OBS_8",
"Factors":{"Genotype":"OBS"},
"Additional sample data":{"RAW_FILE_NAME":"OBS_8","Acronym explanation":"Patients with severe obesity without CKD after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"OBS_9",
"Factors":{"Genotype":"OBS"},
"Additional sample data":{"RAW_FILE_NAME":"OBS_9","Acronym explanation":"Patients with severe obesity without CKD after bariatric surgery"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy24h_1",
"Factors":{"Genotype":"Healthy24h"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy24h_1","Acronym explanation":"Healthy subjects 24h urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy24h_2",
"Factors":{"Genotype":"Healthy24h"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy24h_2","Acronym explanation":"Healthy subjects 24h urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy24h_3",
"Factors":{"Genotype":"Healthy24h"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy24h_3","Acronym explanation":"Healthy subjects 24h urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy24h_4",
"Factors":{"Genotype":"Healthy24h"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy24h_4","Acronym explanation":"Healthy subjects 24h urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy24h_5",
"Factors":{"Genotype":"Healthy24h"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy24h_5","Acronym explanation":"Healthy subjects 24h urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy24h_6",
"Factors":{"Genotype":"Healthy24h"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy24h_6","Acronym explanation":"Healthy subjects 24h urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy24h_7",
"Factors":{"Genotype":"Healthy24h"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy24h_7","Acronym explanation":"Healthy subjects 24h urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy24h_8",
"Factors":{"Genotype":"Healthy24h"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy24h_8","Acronym explanation":"Healthy subjects 24h urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy24h_9",
"Factors":{"Genotype":"Healthy24h"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy24h_9","Acronym explanation":"Healthy subjects 24h urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy24h_10",
"Factors":{"Genotype":"Healthy24h"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy24h_10","Acronym explanation":"Healthy subjects 24h urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy1V_1",
"Factors":{"Genotype":"Healthy1V"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy1V_1","Acronym explanation":"Healthy subjects first morning void urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy1V_2",
"Factors":{"Genotype":"Healthy1V"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy1V_2","Acronym explanation":"Healthy subjects first morning void urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy1V_3",
"Factors":{"Genotype":"Healthy1V"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy1V_3","Acronym explanation":"Healthy subjects first morning void urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy1V_4",
"Factors":{"Genotype":"Healthy1V"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy1V_4","Acronym explanation":"Healthy subjects first morning void urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy1V_5",
"Factors":{"Genotype":"Healthy1V"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy1V_5","Acronym explanation":"Healthy subjects first morning void urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy1V_6",
"Factors":{"Genotype":"Healthy1V"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy1V_6","Acronym explanation":"Healthy subjects first morning void urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy1V_7",
"Factors":{"Genotype":"Healthy1V"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy1V_7","Acronym explanation":"Healthy subjects first morning void urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy1V_8",
"Factors":{"Genotype":"Healthy1V"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy1V_8","Acronym explanation":"Healthy subjects first morning void urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy1V_9",
"Factors":{"Genotype":"Healthy1V"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy1V_9","Acronym explanation":"Healthy subjects first morning void urine"}
},
{
"Subject ID":"-",
"Sample ID":"Healthy1V_10",
"Factors":{"Genotype":"Healthy1V"},
"Additional sample data":{"RAW_FILE_NAME":"Healthy1V_10","Acronym explanation":"Healthy subjects first morning void urine"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CKD_1",
"Factors":{"Genotype":"QC_CKD"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CKD_1","Acronym explanation":"Quality controls CKD patients"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CKD_2",
"Factors":{"Genotype":"QC_CKD"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CKD_2","Acronym explanation":"Quality controls CKD patients"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CKD_3",
"Factors":{"Genotype":"QC_CKD"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CKD_3","Acronym explanation":"Quality controls CKD patients"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CKD_4",
"Factors":{"Genotype":"QC_CKD"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CKD_4","Acronym explanation":"Quality controls CKD patients"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CKD_5",
"Factors":{"Genotype":"QC_CKD"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CKD_5","Acronym explanation":"Quality controls CKD patients"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CKD_6",
"Factors":{"Genotype":"QC_CKD"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CKD_6","Acronym explanation":"Quality controls CKD patients"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CKD_7",
"Factors":{"Genotype":"QC_CKD"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CKD_7","Acronym explanation":"Quality controls CKD patients"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CKD_8",
"Factors":{"Genotype":"QC_CKD"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CKD_8","Acronym explanation":"Quality controls CKD patients"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Control_1",
"Factors":{"Genotype":"QC_Control"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Control_1","Acronym explanation":"Quality controls patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Control_2",
"Factors":{"Genotype":"QC_Control"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Control_2","Acronym explanation":"Quality controls patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Control_3",
"Factors":{"Genotype":"QC_Control"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Control_3","Acronym explanation":"Quality controls patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Control_4",
"Factors":{"Genotype":"QC_Control"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Control_4","Acronym explanation":"Quality controls patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Control_5",
"Factors":{"Genotype":"QC_Control"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Control_5","Acronym explanation":"Quality controls patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Control_6",
"Factors":{"Genotype":"QC_Control"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Control_6","Acronym explanation":"Quality controls patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Control_7",
"Factors":{"Genotype":"QC_Control"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Control_7","Acronym explanation":"Quality controls patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Control_8",
"Factors":{"Genotype":"QC_Control"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Control_8","Acronym explanation":"Quality controls patients with severe obesity without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CC_1",
"Factors":{"Genotype":"QC_CC"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CC_1","Acronym explanation":"Quality controls patients with CKD and without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CC_2",
"Factors":{"Genotype":"QC_CC"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CC_2","Acronym explanation":"Quality controls patients with CKD and without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CC_3",
"Factors":{"Genotype":"QC_CC"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CC_3","Acronym explanation":"Quality controls patients with CKD and without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CC_4",
"Factors":{"Genotype":"QC_CC"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CC_4","Acronym explanation":"Quality controls patients with CKD and without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CC_5",
"Factors":{"Genotype":"QC_CC"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CC_5","Acronym explanation":"Quality controls patients with CKD and without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CC_6",
"Factors":{"Genotype":"QC_CC"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CC_6","Acronym explanation":"Quality controls patients with CKD and without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CC_7",
"Factors":{"Genotype":"QC_CC"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CC_7","Acronym explanation":"Quality controls patients with CKD and without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_CC_8",
"Factors":{"Genotype":"QC_CC"},
"Additional sample data":{"RAW_FILE_NAME":"QC_CC_8","Acronym explanation":"Quality controls patients with CKD and without CKD"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Healthy_1",
"Factors":{"Genotype":"QC_Healthy"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Healthy_1","Acronym explanation":"Quality controls healthy individuals"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Healthy_2",
"Factors":{"Genotype":"QC_Healthy"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Healthy_2","Acronym explanation":"Quality controls healthy individuals"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Healthy_3",
"Factors":{"Genotype":"QC_Healthy"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Healthy_3","Acronym explanation":"Quality controls healthy individuals"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Healthy_4",
"Factors":{"Genotype":"QC_Healthy"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Healthy_4","Acronym explanation":"Quality controls healthy individuals"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Healthy_5",
"Factors":{"Genotype":"QC_Healthy"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Healthy_5","Acronym explanation":"Quality controls healthy individuals"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Healthy_6",
"Factors":{"Genotype":"QC_Healthy"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Healthy_6","Acronym explanation":"Quality controls healthy individuals"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Healthy_7",
"Factors":{"Genotype":"QC_Healthy"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Healthy_7","Acronym explanation":"Quality controls healthy individuals"}
},
{
"Subject ID":"-",
"Sample ID":"QC_Healthy_8",
"Factors":{"Genotype":"QC_Healthy"},
"Additional sample data":{"RAW_FILE_NAME":"QC_Healthy_8","Acronym explanation":"Quality controls healthy individuals"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"First morning void and 24-hour urine samples, were collected, aliquoted and stored at -80°C until extraction. 24-h urine samples were prepared collecting all the urine after the first morning void until the first urine in the next day.","SAMPLE_TYPE":"Urine","COLLECTION_FREQUENCY":"First void and 24h urines","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"CKD patients with severe obesity and non-CKD patients with severe obesity underwent bariatric surgery."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"100 µL of each urine sample were mixed in a 100 µL IS (internal standard) mixture with 0.4 mM of methionine sulfone and 2 mM of paracetamol containing 5% of acetonitrile, then, samples were vortexed. Some CKD patients showed an elevated proteinuria and in order to avoid capillary collapse, a Merck Milipore filters (Ultrafiltration Device with Ultracel YM-T membrane, product 4104) were used. Samples mixed with IS mixture were added to filters and centrifuged (2000 g, 60 min, 4 °C). Then, 90 µl of each sample was transferred to a glass vial. Samples were stored at -20°C until analysis. Stability and reproducibility of the system were checked with quality control (QC) samples. Four types of QC samples were prepared combining 60 µl of each sample after filtration: 1. QC from OD and OD BS samples (QC CKD). 2. QC from O and O BS samples (QC Control). 3. QC from OD, OD BS and O and OD BS samples (QC CC). 4. QC from Healthy 1H and Healthy 24H samples (QC Healthy). QC samples were vortex-mixed, transferred to a vial and stored at -20°C until analysis.","PROCESSING_STORAGE_CONDITIONS":"-80℃","EXTRACT_STORAGE":"On ice"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Analysis were performed in a capillary electrophoresis (CE) system coupled to a TOF-MS analyser (TOF: time-of-flight) purchased from Agilent Technologies (CE: 7100, MS: 6224). In coupling process, steath liquid was supplied by an ISO pump (Agilent 1200) to increase the volatility and compensate the volume for MS through an electrospray source. A fused silica capillary was used to perform metabolite separation (100 cm total length x 50 μm i.d. x 360 μm o.d., provided by Agilent Technologies). Capillary was conditioned with water, BGE and NaOH. Before analysis, capillary was rinsed for 5 min at 950 mbar with BGE and was applied a voltage of 30kV for 10 s to displace BGE ions. After conditioning, sample was loaded applying 50 mbar for 50 s, then, BGE was applied at 100 mbar for 10 s. In the capillary was applied a pressure of 25 mbar and a voltage of 30 kV to achieve metabolite separation. A positively charged spray is formed by a flow of 0.6 mL min−1 (1:100 split) when separated compounds leave the capillary from the auxiliary liquid system with a nebulization pressure of 10 psig with nitrogen and a capillary voltage of 3500 V. A hot nitrogen flow of 10 mL min−1 at 200 °C was applied to dry the spray.","CHROMATOGRAPHY_TYPE":"None (Direct infusion)","INSTRUMENT_NAME":"none","COLUMN_NAME":"none"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 6224 TOF","INSTRUMENT_TYPE":"CE-TOF-MS","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"Ions in gaseous state were directed to the TOF using two different voltages for the fragmentor (125 V and 200 V) and voltages of 65 V and 750 V for the skimmer and the octopole, respectively. Mass range was set from 70 to 1050 Da at a scanning rate of 1.02 scans per second. Data was collected in ESI positive ion mode. MassHunter Workstation version B.06.01 (Agilent Technologies) was used to monitor CE-MS analysis. Voltage of 125 V in the fragmentor was applied to ordinary samples and QC control samples. Data acquired at 125 V, equivalent to MS1, provided information about adducts, isotopes, multimers and fragments ions with low intensity. Voltage of 200 V in the fragmentor was applied to four QC (covering the four types of QC prepared). Data acquired at 200 V is similar to data obtained in 10 eV MS/MS, although has been previously reported that some fragment ions could differ. An equilibration QC was prepared to adapt and prepare the equipment for the conditions of the samples to be analysed. Equilibration QC was prepared as a pool of the four types of QC used to cover completely the biological diversity of the samples. Equilibration QC was analysed before samples. QC samples were disposed throughout the instrumental analysis.","MS_RESULTS_FILE":"ST001943_AN003187_Results.txt UNITS:Area Has m/z:Neutral masses Has RT:Yes RT units:Minutes"}

}