#METABOLOMICS WORKBENCH harleygordon_20211029_134313 DATATRACK_ID:2913 STUDY_ID:ST001962 ANALYSIS_ID:AN003198 PROJECT_ID:PR001249 VERSION 1 CREATED_ON November 3, 2021, 11:41 am #PROJECT PR:PROJECT_TITLE Nontargeted metabolomics of UGT71 CRISPR knockouts in Poplar PR:PROJECT_TYPE non-targeted metabolomics PR:PROJECT_SUMMARY HRMS non-targeted analysis of knockout, empty vector, and wild-type populus PR:PROJECT_SUMMARY tremula x populus alba plants. CRISPR-Cas9 knockout gene was UGT71L1 and mutants PR:PROJECT_SUMMARY were selected with Kanamycin resistance. PR:INSTITUTE University of Victoria PR:DEPARTMENT Biology PR:LABORATORY Constabel PR:LAST_NAME Gordon PR:FIRST_NAME Harley PR:ADDRESS 3800 Finnerty Rd PR:EMAIL harleygordon@uvic.ca PR:PHONE 2507217211 #STUDY ST:STUDY_TITLE Nontargeted metabolomics of UGT71 CRISPR knockouts in Poplar ST:STUDY_SUMMARY Non-targeted metabolomic analysis of UGT71L1 CRISPR-Cas9 knockouts in populus ST:STUDY_SUMMARY tremula x populus alba ST:INSTITUTE University of Victoria ST:LAST_NAME Gordon ST:FIRST_NAME Harley ST:ADDRESS 3800 Finnerty Rd ST:EMAIL harleygordon@uvic.ca ST:PHONE 2507217211 #SUBJECT SU:SUBJECT_TYPE Plant SU:SUBJECT_SPECIES Poplar #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - Salicortin sample_type:Standard RAW_FILE_NAME=Salicortin SUBJECT_SAMPLE_FACTORS - Tremulacin sample_type:Standard RAW_FILE_NAME=Tremulacin SUBJECT_SAMPLE_FACTORS - Salicylic_Alcohol sample_type:Standard RAW_FILE_NAME=Salicylic_Alcohol SUBJECT_SAMPLE_FACTORS - Salicyl_Aldehyde sample_type:Standard RAW_FILE_NAME=Salicyl_Aldehyde SUBJECT_SAMPLE_FACTORS - Helicin sample_type:Standard RAW_FILE_NAME=Helicin SUBJECT_SAMPLE_FACTORS - Salicylic_Acid sample_type:Standard RAW_FILE_NAME=Salicylic_Acid SUBJECT_SAMPLE_FACTORS - Salicin sample_type:Standard RAW_FILE_NAME=Salicin SUBJECT_SAMPLE_FACTORS - Tremuloidin sample_type:Standard RAW_FILE_NAME=Tremuloidin SUBJECT_SAMPLE_FACTORS - P-coumaric_acid sample_type:Standard RAW_FILE_NAME=P-coumaric_acid SUBJECT_SAMPLE_FACTORS - Chlorogenic_Acid sample_type:Standard RAW_FILE_NAME=Chlorogenic_Acid SUBJECT_SAMPLE_FACTORS - Caffeic_Acid sample_type:Standard RAW_FILE_NAME=Caffeic_Acid SUBJECT_SAMPLE_FACTORS - Ferrulic_Acid sample_type:Standard RAW_FILE_NAME=Ferrulic_Acid SUBJECT_SAMPLE_FACTORS - Epi-catechin sample_type:Standard RAW_FILE_NAME=Epi-catechin SUBJECT_SAMPLE_FACTORS - Benzoic_Acid sample_type:Standard RAW_FILE_NAME=Benzoic_Acid SUBJECT_SAMPLE_FACTORS - Catechol sample_type:Standard RAW_FILE_NAME=Catechol SUBJECT_SAMPLE_FACTORS - 4-methoxyphenol sample_type:Standard RAW_FILE_NAME=4-methoxyphenol SUBJECT_SAMPLE_FACTORS - Salicyl_Benzoate sample_type:Standard RAW_FILE_NAME=Salicyl_Benzoate SUBJECT_SAMPLE_FACTORS - 71-5_1a sample_type:knockout RAW_FILE_NAME=71-5_1a SUBJECT_SAMPLE_FACTORS - 71-5_1b sample_type:knockout RAW_FILE_NAME=71-5_1b SUBJECT_SAMPLE_FACTORS - 71-5_1c sample_type:knockout RAW_FILE_NAME=71-5_1c SUBJECT_SAMPLE_FACTORS - 71-5_1blank sample_type:Blank RAW_FILE_NAME=71-5_1blank SUBJECT_SAMPLE_FACTORS - 71-5_2a sample_type:knockout RAW_FILE_NAME=71-5_2a SUBJECT_SAMPLE_FACTORS - 71-5_2b sample_type:knockout RAW_FILE_NAME=71-5_2b SUBJECT_SAMPLE_FACTORS - 71-5_2c sample_type:knockout RAW_FILE_NAME=71-5_2c SUBJECT_SAMPLE_FACTORS - 71-5_2blank sample_type:Blank RAW_FILE_NAME=71-5_2blank SUBJECT_SAMPLE_FACTORS - 71-5_4a sample_type:knockout RAW_FILE_NAME=71-5_4a SUBJECT_SAMPLE_FACTORS - 71-5_4b sample_type:knockout RAW_FILE_NAME=71-5_4b SUBJECT_SAMPLE_FACTORS - 71-5_4c sample_type:knockout RAW_FILE_NAME=71-5_4c SUBJECT_SAMPLE_FACTORS - 71-5_4blank sample_type:Blank RAW_FILE_NAME=71-5_4blank SUBJECT_SAMPLE_FACTORS - 71-5_5a sample_type:knockout RAW_FILE_NAME=71-5_5a SUBJECT_SAMPLE_FACTORS - 71-5_5b sample_type:knockout RAW_FILE_NAME=71-5_5b SUBJECT_SAMPLE_FACTORS - 71-5_5c sample_type:knockout RAW_FILE_NAME=71-5_5c SUBJECT_SAMPLE_FACTORS - 71-5_5blank sample_type:Blank RAW_FILE_NAME=71-5_5blank SUBJECT_SAMPLE_FACTORS - 71-5_7a sample_type:knockout RAW_FILE_NAME=71-5_7a SUBJECT_SAMPLE_FACTORS - 71-5_7b sample_type:knockout RAW_FILE_NAME=71-5_7b SUBJECT_SAMPLE_FACTORS - 71-5_7c sample_type:knockout RAW_FILE_NAME=71-5_7c SUBJECT_SAMPLE_FACTORS - 71-5_7blank sample_type:Blank RAW_FILE_NAME=71-5_7blank SUBJECT_SAMPLE_FACTORS - 71-3_12a sample_type:knockout RAW_FILE_NAME=71-3_12a SUBJECT_SAMPLE_FACTORS - 71-3_12b sample_type:knockout RAW_FILE_NAME=71-3_12b SUBJECT_SAMPLE_FACTORS - 71-3_12c sample_type:knockout RAW_FILE_NAME=71-3_12c SUBJECT_SAMPLE_FACTORS - 71-3_12blank sample_type:Blank RAW_FILE_NAME=71-3_12blank SUBJECT_SAMPLE_FACTORS - 71-3_13a sample_type:knockout RAW_FILE_NAME=71-3_13a SUBJECT_SAMPLE_FACTORS - 71-3_13b sample_type:knockout RAW_FILE_NAME=71-3_13b SUBJECT_SAMPLE_FACTORS - 71-3_13c sample_type:knockout RAW_FILE_NAME=71-3_13c SUBJECT_SAMPLE_FACTORS - 71-3_13blank sample_type:Blank RAW_FILE_NAME=71-3_13blank SUBJECT_SAMPLE_FACTORS - 71-3_14a sample_type:knockout RAW_FILE_NAME=71-3_14a SUBJECT_SAMPLE_FACTORS - 71-3_14b sample_type:knockout RAW_FILE_NAME=71-3_14b SUBJECT_SAMPLE_FACTORS - 71-3_14c sample_type:knockout RAW_FILE_NAME=71-3_14c SUBJECT_SAMPLE_FACTORS - 71-3_14blank sample_type:Blank RAW_FILE_NAME=71-3_14blank SUBJECT_SAMPLE_FACTORS - 71-3_15a sample_type:knockout RAW_FILE_NAME=71-3_15a SUBJECT_SAMPLE_FACTORS - 71-3_15b sample_type:knockout RAW_FILE_NAME=71-3_15b SUBJECT_SAMPLE_FACTORS - 71-3_15c sample_type:knockout RAW_FILE_NAME=71-3_15c SUBJECT_SAMPLE_FACTORS - 71-3_15blank sample_type:Blank RAW_FILE_NAME=71-3_15blank SUBJECT_SAMPLE_FACTORS - 71-3_16a sample_type:knockout RAW_FILE_NAME=71-3_16a SUBJECT_SAMPLE_FACTORS - 71-3_16b sample_type:knockout RAW_FILE_NAME=71-3_16b SUBJECT_SAMPLE_FACTORS - 71-3_16c sample_type:knockout RAW_FILE_NAME=71-3_16c SUBJECT_SAMPLE_FACTORS - 71-3_16blank sample_type:Blank RAW_FILE_NAME=71-3_16blank SUBJECT_SAMPLE_FACTORS - 71-10_21a sample_type:intermediate RAW_FILE_NAME=71-10_21a SUBJECT_SAMPLE_FACTORS - 71-10_21b sample_type:intermediate RAW_FILE_NAME=71-10_21b SUBJECT_SAMPLE_FACTORS - 71-10_21c sample_type:intermediate RAW_FILE_NAME=71-10_21c SUBJECT_SAMPLE_FACTORS - 71-10_21blank sample_type:Blank RAW_FILE_NAME=71-10_21blank SUBJECT_SAMPLE_FACTORS - 71-10_22a sample_type:intermediate RAW_FILE_NAME=71-10_22a SUBJECT_SAMPLE_FACTORS - 71-10_22b sample_type:intermediate RAW_FILE_NAME=71-10_22b SUBJECT_SAMPLE_FACTORS - 71-10_22c sample_type:intermediate RAW_FILE_NAME=71-10_22c SUBJECT_SAMPLE_FACTORS - 71-10_22blank sample_type:Blank RAW_FILE_NAME=71-10_22blank SUBJECT_SAMPLE_FACTORS - 71-10_23a sample_type:intermediate RAW_FILE_NAME=71-10_23a SUBJECT_SAMPLE_FACTORS - 71-10_23b sample_type:intermediate RAW_FILE_NAME=71-10_23b SUBJECT_SAMPLE_FACTORS - 71-10_23c sample_type:intermediate RAW_FILE_NAME=71-10_23c SUBJECT_SAMPLE_FACTORS - 71-10_23blank sample_type:Blank RAW_FILE_NAME=71-10_23blank SUBJECT_SAMPLE_FACTORS - 71-10_24a sample_type:intermediate RAW_FILE_NAME=71-10_24a SUBJECT_SAMPLE_FACTORS - 71-10_24b sample_type:intermediate RAW_FILE_NAME=71-10_24b SUBJECT_SAMPLE_FACTORS - 71-10_24c sample_type:intermediate RAW_FILE_NAME=71-10_24c SUBJECT_SAMPLE_FACTORS - 71-10_24blank sample_type:Blank RAW_FILE_NAME=71-10_24blank SUBJECT_SAMPLE_FACTORS - 71-10_26a sample_type:intermediate RAW_FILE_NAME=71-10_26a SUBJECT_SAMPLE_FACTORS - 71-10_26b sample_type:intermediate RAW_FILE_NAME=71-10_26b SUBJECT_SAMPLE_FACTORS - 71-10_26c sample_type:intermediate RAW_FILE_NAME=71-10_26c SUBJECT_SAMPLE_FACTORS - 71-10_26blank sample_type:Blank RAW_FILE_NAME=71-10_26blank SUBJECT_SAMPLE_FACTORS - 71-9_33a sample_type:knockout RAW_FILE_NAME=71-9_33a SUBJECT_SAMPLE_FACTORS - 71-9_33b sample_type:knockout RAW_FILE_NAME=71-9_33b SUBJECT_SAMPLE_FACTORS - 71-9_33c sample_type:knockout RAW_FILE_NAME=71-9_33c SUBJECT_SAMPLE_FACTORS - 71-9_33blank sample_type:Blank RAW_FILE_NAME=71-9_33blank SUBJECT_SAMPLE_FACTORS - 71-9_35a sample_type:knockout RAW_FILE_NAME=71-9_35a SUBJECT_SAMPLE_FACTORS - 71-9_35b sample_type:knockout RAW_FILE_NAME=71-9_35b SUBJECT_SAMPLE_FACTORS - 71-9_35c sample_type:knockout RAW_FILE_NAME=71-9_35c SUBJECT_SAMPLE_FACTORS - 71-9_35blank sample_type:Blank RAW_FILE_NAME=71-9_35blank SUBJECT_SAMPLE_FACTORS - 71-9_36a sample_type:knockout RAW_FILE_NAME=71-9_36a SUBJECT_SAMPLE_FACTORS - 71-9_36b sample_type:knockout RAW_FILE_NAME=71-9_36b SUBJECT_SAMPLE_FACTORS - 71-9_36c sample_type:knockout RAW_FILE_NAME=71-9_36c SUBJECT_SAMPLE_FACTORS - 71-9_36blank sample_type:Blank RAW_FILE_NAME=71-9_36blank SUBJECT_SAMPLE_FACTORS - 71-9_38a sample_type:knockout RAW_FILE_NAME=71-9_38a SUBJECT_SAMPLE_FACTORS - 71-9_38b sample_type:knockout RAW_FILE_NAME=71-9_38b SUBJECT_SAMPLE_FACTORS - 71-9_38c sample_type:knockout RAW_FILE_NAME=71-9_38c SUBJECT_SAMPLE_FACTORS - 71-9_38blank sample_type:Blank RAW_FILE_NAME=71-9_38blank SUBJECT_SAMPLE_FACTORS - 71-9_39a sample_type:knockout RAW_FILE_NAME=71-9_39a SUBJECT_SAMPLE_FACTORS - 71-9_39b sample_type:knockout RAW_FILE_NAME=71-9_39b SUBJECT_SAMPLE_FACTORS - 71-9_39c sample_type:knockout RAW_FILE_NAME=71-9_39c SUBJECT_SAMPLE_FACTORS - 71-9_39blank sample_type:Blank RAW_FILE_NAME=71-9_39blank SUBJECT_SAMPLE_FACTORS - Empty-2_42a sample_type:empty_vector RAW_FILE_NAME=Empty-2_42a SUBJECT_SAMPLE_FACTORS - Empty-2_42b sample_type:empty_vector RAW_FILE_NAME=Empty-2_42b SUBJECT_SAMPLE_FACTORS - Empty-2_42c sample_type:empty_vector RAW_FILE_NAME=Empty-2_42c SUBJECT_SAMPLE_FACTORS - Empty-2_42blank sample_type:Blank RAW_FILE_NAME=Empty-2_42blank SUBJECT_SAMPLE_FACTORS - Empty-2_43a sample_type:empty_vector RAW_FILE_NAME=Empty-2_43a SUBJECT_SAMPLE_FACTORS - Empty-2_43b sample_type:empty_vector RAW_FILE_NAME=Empty-2_43b SUBJECT_SAMPLE_FACTORS - Empty-2_43c sample_type:empty_vector RAW_FILE_NAME=Empty-2_43c SUBJECT_SAMPLE_FACTORS - Empty-2_43blank sample_type:Blank RAW_FILE_NAME=Empty-2_43blank SUBJECT_SAMPLE_FACTORS - Empty-2_44a sample_type:empty_vector RAW_FILE_NAME=Empty-2_44a SUBJECT_SAMPLE_FACTORS - Empty-2_44b sample_type:empty_vector RAW_FILE_NAME=Empty-2_44b SUBJECT_SAMPLE_FACTORS - Empty-2_44c sample_type:empty_vector RAW_FILE_NAME=Empty-2_44c SUBJECT_SAMPLE_FACTORS - Empty-2_44blank sample_type:Blank RAW_FILE_NAME=Empty-2_44blank SUBJECT_SAMPLE_FACTORS - Empty-2_45a sample_type:empty_vector RAW_FILE_NAME=Empty-2_45a SUBJECT_SAMPLE_FACTORS - Empty-2_45b sample_type:empty_vector RAW_FILE_NAME=Empty-2_45b SUBJECT_SAMPLE_FACTORS - Empty-2_45c sample_type:empty_vector RAW_FILE_NAME=Empty-2_45c SUBJECT_SAMPLE_FACTORS - Empty-2_45blank sample_type:Blank RAW_FILE_NAME=Empty-2_45blank SUBJECT_SAMPLE_FACTORS - Empty-2_46a sample_type:empty_vector RAW_FILE_NAME=Empty-2_46a SUBJECT_SAMPLE_FACTORS - Empty-2_46b sample_type:empty_vector RAW_FILE_NAME=Empty-2_46b SUBJECT_SAMPLE_FACTORS - Empty-2_46c sample_type:empty_vector RAW_FILE_NAME=Empty-2_46c SUBJECT_SAMPLE_FACTORS - Empty-2_46blank sample_type:Blank RAW_FILE_NAME=Empty-2_46blank SUBJECT_SAMPLE_FACTORS - Empty-5_49a sample_type:empty_vector RAW_FILE_NAME=Empty-5_49a SUBJECT_SAMPLE_FACTORS - Empty-5_49b sample_type:empty_vector RAW_FILE_NAME=Empty-5_49b SUBJECT_SAMPLE_FACTORS - Empty-5_49c sample_type:empty_vector RAW_FILE_NAME=Empty-5_49c SUBJECT_SAMPLE_FACTORS - Empty-5_49blank sample_type:Blank RAW_FILE_NAME=Empty-5_49blank SUBJECT_SAMPLE_FACTORS - Empty-5_50a sample_type:empty_vector RAW_FILE_NAME=Empty-5_50a SUBJECT_SAMPLE_FACTORS - Empty-5_50b sample_type:empty_vector RAW_FILE_NAME=Empty-5_50b SUBJECT_SAMPLE_FACTORS - Empty-5_50c sample_type:empty_vector RAW_FILE_NAME=Empty-5_50c SUBJECT_SAMPLE_FACTORS - Empty-5_50blank sample_type:Blank RAW_FILE_NAME=Empty-5_50blank SUBJECT_SAMPLE_FACTORS - Empty-5_51a sample_type:empty_vector RAW_FILE_NAME=Empty-5_51a SUBJECT_SAMPLE_FACTORS - Empty-5_51b sample_type:empty_vector RAW_FILE_NAME=Empty-5_51b SUBJECT_SAMPLE_FACTORS - Empty-5_51c sample_type:empty_vector RAW_FILE_NAME=Empty-5_51c SUBJECT_SAMPLE_FACTORS - Empty-5_51blank sample_type:Blank RAW_FILE_NAME=Empty-5_51blank SUBJECT_SAMPLE_FACTORS - Empty-5_52a sample_type:empty_vector RAW_FILE_NAME=Empty-5_52a SUBJECT_SAMPLE_FACTORS - Empty-5_52b sample_type:empty_vector RAW_FILE_NAME=Empty-5_52b SUBJECT_SAMPLE_FACTORS - Empty-5_52c sample_type:empty_vector RAW_FILE_NAME=Empty-5_52c SUBJECT_SAMPLE_FACTORS - Empty-5_52blank sample_type:Blank RAW_FILE_NAME=Empty-5_52blank SUBJECT_SAMPLE_FACTORS - Empty-5_53a sample_type:empty_vector RAW_FILE_NAME=Empty-5_53a SUBJECT_SAMPLE_FACTORS - Empty-5_53b sample_type:empty_vector RAW_FILE_NAME=Empty-5_53b SUBJECT_SAMPLE_FACTORS - Empty-5_53c sample_type:empty_vector RAW_FILE_NAME=Empty-5_53c SUBJECT_SAMPLE_FACTORS - Empty-5_53blank sample_type:Blank RAW_FILE_NAME=Empty-5_53blank SUBJECT_SAMPLE_FACTORS - Empty-6_55a sample_type:empty_vector RAW_FILE_NAME=Empty-6_55a SUBJECT_SAMPLE_FACTORS - Empty-6_55b sample_type:empty_vector RAW_FILE_NAME=Empty-6_55b SUBJECT_SAMPLE_FACTORS - Empty-6_55c sample_type:empty_vector RAW_FILE_NAME=Empty-6_55c SUBJECT_SAMPLE_FACTORS - Empty-6_55blank sample_type:Blank RAW_FILE_NAME=Empty-6_55blank SUBJECT_SAMPLE_FACTORS - Empty-6_56a sample_type:empty_vector RAW_FILE_NAME=Empty-6_56a SUBJECT_SAMPLE_FACTORS - Empty-6_56b sample_type:empty_vector RAW_FILE_NAME=Empty-6_56b SUBJECT_SAMPLE_FACTORS - Empty-6_56c sample_type:empty_vector RAW_FILE_NAME=Empty-6_56c SUBJECT_SAMPLE_FACTORS - Empty-6_56blank sample_type:Blank RAW_FILE_NAME=Empty-6_56blank SUBJECT_SAMPLE_FACTORS - Empty-6_58a sample_type:empty_vector RAW_FILE_NAME=Empty-6_58a SUBJECT_SAMPLE_FACTORS - Empty-6_58b sample_type:empty_vector RAW_FILE_NAME=Empty-6_58b SUBJECT_SAMPLE_FACTORS - Empty-6_58c sample_type:empty_vector RAW_FILE_NAME=Empty-6_58c SUBJECT_SAMPLE_FACTORS - Empty-6_58blank sample_type:Blank RAW_FILE_NAME=Empty-6_58blank SUBJECT_SAMPLE_FACTORS - Empty-6_59a sample_type:empty_vector RAW_FILE_NAME=Empty-6_59a SUBJECT_SAMPLE_FACTORS - Empty-6_59b sample_type:empty_vector RAW_FILE_NAME=Empty-6_59b SUBJECT_SAMPLE_FACTORS - Empty-6_59c sample_type:empty_vector RAW_FILE_NAME=Empty-6_59c SUBJECT_SAMPLE_FACTORS - Empty-6_59blank sample_type:Blank RAW_FILE_NAME=Empty-6_59blank SUBJECT_SAMPLE_FACTORS - Empty-6_61a sample_type:empty_vector RAW_FILE_NAME=Empty-6_61a SUBJECT_SAMPLE_FACTORS - Empty-6_61b sample_type:empty_vector RAW_FILE_NAME=Empty-6_61b SUBJECT_SAMPLE_FACTORS - Empty-6_61c sample_type:empty_vector RAW_FILE_NAME=Empty-6_61c SUBJECT_SAMPLE_FACTORS - Empty-6_61blank sample_type:Blank RAW_FILE_NAME=Empty-6_61blank SUBJECT_SAMPLE_FACTORS - WT_64a sample_type:wild_type RAW_FILE_NAME=WT_64a SUBJECT_SAMPLE_FACTORS - WT_64b sample_type:wild_type RAW_FILE_NAME=WT_64b SUBJECT_SAMPLE_FACTORS - WT_64c sample_type:wild_type RAW_FILE_NAME=WT_64c SUBJECT_SAMPLE_FACTORS - WT_64blank sample_type:Blank RAW_FILE_NAME=WT_64blank SUBJECT_SAMPLE_FACTORS - WT_65a sample_type:wild_type RAW_FILE_NAME=WT_65a SUBJECT_SAMPLE_FACTORS - WT_65b sample_type:wild_type RAW_FILE_NAME=WT_65b SUBJECT_SAMPLE_FACTORS - WT_65c sample_type:wild_type RAW_FILE_NAME=WT_65c SUBJECT_SAMPLE_FACTORS - WT_65blank sample_type:Blank RAW_FILE_NAME=WT_65blank SUBJECT_SAMPLE_FACTORS - WT_66a sample_type:wild_type RAW_FILE_NAME=WT_66a SUBJECT_SAMPLE_FACTORS - WT_66b sample_type:wild_type RAW_FILE_NAME=WT_66b SUBJECT_SAMPLE_FACTORS - WT_66c sample_type:wild_type RAW_FILE_NAME=WT_66c SUBJECT_SAMPLE_FACTORS - WT_66blank sample_type:Blank RAW_FILE_NAME=WT_66blank SUBJECT_SAMPLE_FACTORS - WT_67a sample_type:wild_type RAW_FILE_NAME=WT_67a SUBJECT_SAMPLE_FACTORS - WT_67b sample_type:wild_type RAW_FILE_NAME=WT_67b SUBJECT_SAMPLE_FACTORS - WT_67c sample_type:wild_type RAW_FILE_NAME=WT_67c SUBJECT_SAMPLE_FACTORS - WT_67blank sample_type:Blank RAW_FILE_NAME=WT_67blank SUBJECT_SAMPLE_FACTORS - WT_69a sample_type:wild_type RAW_FILE_NAME=WT_69a SUBJECT_SAMPLE_FACTORS - WT_69b sample_type:wild_type RAW_FILE_NAME=WT_69b SUBJECT_SAMPLE_FACTORS - WT_69c sample_type:wild_type RAW_FILE_NAME=WT_69c SUBJECT_SAMPLE_FACTORS - WT_69blank sample_type:Blank RAW_FILE_NAME=WT_69blank #COLLECTION CO:COLLECTION_SUMMARY Samples are fully expanded leaf tissue with midvein excised of three month old CO:COLLECTION_SUMMARY greenhouse grown poplar. Tissue was flash frozen and stored at -80 prior to CO:COLLECTION_SUMMARY metabolite extraction. CO:SAMPLE_TYPE Leaves #TREATMENT TR:TREATMENT_SUMMARY Leaf tissue from Populus tremula x Populus alba was transformed using TR:TREATMENT_SUMMARY CRISPR-Cas9 to create functional knockouts of the UGT71L1 gene. Positive TR:TREATMENT_SUMMARY transformants were selected with Kanamycin and sequencing was conducted to TR:TREATMENT_SUMMARY confirm transformation. Clones of either wild type plants, knockouts (frame TR:TREATMENT_SUMMARY shift mutations, both alleles), intermediates (Frameshift mutation, one allele), TR:TREATMENT_SUMMARY or empty vectors (no gRNA for Cas9 construct). Plants were then grown under TR:TREATMENT_SUMMARY identical conditions for three months in a greenhouse. TR:TREATMENT Genetic TR:PLANT_GROWTH_LOCATION Greenhouse TR:PLANT_WATERING_REGIME As required #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Flash frozen leaf tissue was homogenized under liquid nitrogen with mortar and SP:SAMPLEPREP_SUMMARY pestle. Tissue was then freeze dried and stored at room temperature prior to SP:SAMPLEPREP_SUMMARY analysis. For extraction, 50 mg of freeze-dried tissue was weighed into a 2 mL SP:SAMPLEPREP_SUMMARY cryo-vial with 4 x 5 mm steel ball bearings and 750 mL of chilled methanol. SP:SAMPLEPREP_SUMMARY Samples were homogenized 2 x 45 seconds at 5500 rpm using a Precellys 24 SP:SAMPLEPREP_SUMMARY Homogenizer, then sonicated for 2 minutes in a sonicating water bath. Samples SP:SAMPLEPREP_SUMMARY were centrifuged at 12,000 rpm for 2 minutes and the supernatant was filtered SP:SAMPLEPREP_SUMMARY through 0.22 µm PTFE filters into glass vials. Methanol extractions were SP:SAMPLEPREP_SUMMARY repeated twice and extracts were pooled. Extracts were dried under vacuum at SP:SAMPLEPREP_SUMMARY ambient temperature. Final extraction weight was determined, vials were sealed SP:SAMPLEPREP_SUMMARY and stored at -20°C until analysis. Each biological replicate included an SP:SAMPLEPREP_SUMMARY extraction #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 CH:COLUMN_NAME Phenomenex Kinitex C18 (50 mm x 2.1 mm, 1.7 um) CH:FLOW_RATE 0.35 ml/min #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo LTQ XL MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS voltage of 35 V m/z from 100 – 2000.00 MS1 data was processed using MZMine2 MS:MS_RESULTS_FILE ST001962_AN003198_Results.txt UNITS:Area Has m/z:Yes Has RT:Yes RT units:Minutes #END