{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001963","ANALYSIS_ID":"AN003200","VERSION":"1","CREATED_ON":"November 3, 2021, 11:48 am"},

"PROJECT":{"PROJECT_TITLE":"THEM6-mediated lipid remodelling sustains stress resistance in cancer","PROJECT_TYPE":"Lipidomics","PROJECT_SUMMARY":"Despite the clinical benefit of androgen-deprivation therapy (ADT), the majority of patients with advanced prostate cancer (PCa) ultimately develop lethal castration-resistant prostate cancer (CRPC). In this study, we identified thioesterase superfamily member 6 (THEM6) as a marker of ADT resistance in PCa. In patients, THEM6 expression correlates with progressive disease and is associated with poor survival. THEM6 deletion reduces in vivo tumour growth and restores castration sensitivity in orthograft models of CRPC. Mechanistically, THEM6 is located at the endoplasmic reticulum (ER) membrane and controls lipid homeostasis by regulating intracellular levels of ether lipids. As a consequence, THEM6 loss in CRPC cells significantly alters ER function, preventing lipid-mediated induction of ATF4 and reducing de novo sterol biosynthesis. Finally, we show that THEM6 is required for the establishment of the MYC-induced stress response. Thus, similar to PCa, THEM6 loss significantly impairs tumorigenesis in the MYC-dependent subtype of triple negative breast cancer. Altogether our results highlight THEM6 as a novel component of the treatment-induced stress response and a promising target for the treatment of CRPC and MYC-driven cancer.","INSTITUTE":"Beatson Institute for Cancer Research","LAST_NAME":"Rodriguez Blanco","FIRST_NAME":"Giovanny","ADDRESS":"Crewe Road South, Edinburgh, Midlothian, EH42XU, United Kingdom","EMAIL":"g.blanco@ed.ac.uk","PHONE":"00447526056849"},

"STUDY":{"STUDY_TITLE":"THEM6-mediated lipid remodelling sustains stress resistance in cancer","STUDY_TYPE":"Lipidomics","STUDY_SUMMARY":"Despite the clinical benefit of androgen-deprivation therapy (ADT), the majority of patients with advanced prostate cancer (PCa) ultimately develop lethal castration-resistant prostate cancer (CRPC). In this study, we identified thioesterase superfamily member 6 (THEM6) as a marker of ADT resistance in PCa. In patients, THEM6 expression correlates with progressive disease and is associated with poor survival. THEM6 deletion reduces in vivo tumour growth and restores castration sensitivity in orthograft models of CRPC. Mechanistically, THEM6 is located at the endoplasmic reticulum (ER) membrane and controls lipid homeostasis by regulating intracellular levels of ether lipids. As a consequence, THEM6 loss in CRPC cells significantly alters ER function, preventing lipid-mediated induction of ATF4 and reducing de novo sterol biosynthesis. Finally, we show that THEM6 is required for the establishment of the MYC-induced stress response. Thus, similar to PCa, THEM6 loss significantly impairs tumorigenesis in the MYC-dependent subtype of triple negative breast cancer. Altogether our results highlight THEM6 as a novel component of the treatment-induced stress response and a promising target for the treatment of CRPC and MYC-driven cancer.","INSTITUTE":"IGMM","LAST_NAME":"Blanco","FIRST_NAME":"Giovanny","ADDRESS":"Crewe Road South","EMAIL":"g.blanco@ed.ac.uk","PHONE":"+447526056849"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"T0_1_Al_exp1",
"Factors":{"Condition":"T0_1_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T0_1_Al_expt1_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T0_1_Al_exp2",
"Factors":{"Condition":"T0_1_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T0_1_Al_expt2_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T0_1_Al_exp3",
"Factors":{"Condition":"T0_1_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T0_1_Al_expt3_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T0_2_Al_exp1",
"Factors":{"Condition":"T0_2_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T0_2_Al_expt1_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T0_2_Al_exp2",
"Factors":{"Condition":"T0_2_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T0_2_Al_expt2_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T0_2_Al_exp3",
"Factors":{"Condition":"T0_2_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T0_2_Al_expt3_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T0_3_Al_exp1",
"Factors":{"Condition":"T0_3_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T0_3_Al_expt1_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T0_3_Al_exp2",
"Factors":{"Condition":"T0_3_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T0_3_Al_expt2_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T0_3_Al_exp3",
"Factors":{"Condition":"T0_3_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T0_3_Al_expt3_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T1_1_Al_exp1",
"Factors":{"Condition":"T1_1_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T1_1_Al_expt1_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T1_1_Al_exp2",
"Factors":{"Condition":"T1_1_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T1_1_Al_expt2_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T1_1_Al_exp3",
"Factors":{"Condition":"T1_1_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T1_1_Al_expt3_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T1_2_Al_exp1",
"Factors":{"Condition":"T1_2_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T1_2_Al_expt1_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T1_2_Al_exp2",
"Factors":{"Condition":"T1_2_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T1_2_Al_expt2_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T1_2_Al_exp3",
"Factors":{"Condition":"T1_2_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T1_2_Al_expt3_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T1_3_Al_exp1",
"Factors":{"Condition":"T1_3_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T1_3_Al_expt1_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T1_3_Al_exp2",
"Factors":{"Condition":"T1_3_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T1_3_Al_expt2_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T1_3_Al_exp3",
"Factors":{"Condition":"T1_3_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T1_3_Al_expt3_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T7_1_Al_exp1",
"Factors":{"Condition":"T7_1_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T7_1_Al_expt1_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T7_1_Al_exp2",
"Factors":{"Condition":"T7_1_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T7_1_Al_expt2_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T7_1_Al_exp3",
"Factors":{"Condition":"T7_1_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T7_1_Al_expt3_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T7_2_Al_exp1",
"Factors":{"Condition":"T7_2_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T7_2_Al_expt1_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T7_2_Al_exp2",
"Factors":{"Condition":"T7_2_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T7_2_Al_expt2_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T7_2_Al_exp3",
"Factors":{"Condition":"T7_2_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T7_2_Al_expt3_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T7_3_Al_exp1",
"Factors":{"Condition":"T7_3_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T7_3_Al_expt1_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T7_3_Al_exp2",
"Factors":{"Condition":"T7_3_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T7_3_Al_expt2_pos.raw"}
},
{
"Subject ID":"-",
"Sample ID":"T7_3_Al_exp3",
"Factors":{"Condition":"T7_3_Al"},
"Additional sample data":{"RAW_FILE_NAME":"T7_3_Al_expt3_pos.raw"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Lipids were extracted monophasic extractions of a mixture 1:1 of butanol-methanol (BuMe)","SAMPLE_TYPE":"LnCap cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"These cells are CRISPR KOs of THEM6."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Monophasic extraction straight from cell plates using BuMe or IPA."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Please see methods","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"Waters Acquity CSH C18 (100 x 2.1mm, 1.7um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"See Methods file."},

"MS_METABOLITE_DATA":{
"Units":"Peak area",

"Data":[{"Metabolite":"AlkanylTG (O-16:0_14:0_16:0)a","T0_1_Al_exp1":"-3.079.561.234","T0_1_Al_exp2":"-3.126.070.023","T0_1_Al_exp3":"-2.375.190.735","T0_2_Al_exp1":"-3.116.056.442","T0_2_Al_exp2":"-3.347.764.969","T0_2_Al_exp3":"-199.798.584","T0_3_Al_exp1":"-282.491.684","T0_3_Al_exp2":"-3.047.422.409","T0_3_Al_exp3":"-2.380.975.723","T1_1_Al_exp1":"-4.546.678.543","T1_1_Al_exp2":"-4.454.595.566","T1_1_Al_exp3":"-3.335.519.791","T1_2_Al_exp1":"-4.660.432.816","T1_2_Al_exp2":"-4.827.749.252","T1_2_Al_exp3":"-3.444.904.327","T1_3_Al_exp1":"-4.605.413.437","T1_3_Al_exp2":"-4.118.722.916","T1_3_Al_exp3":"-3.432.794.571","T7_1_Al_exp1":"-3.812.498.093","T7_1_Al_exp2":"-3.527.086.258","T7_1_Al_exp3":"-2.858.917.236","T7_2_Al_exp1":"-392.285.347","T7_2_Al_exp2":"-372.759.819","T7_2_Al_exp3":"-2.572.742.462","T7_3_Al_exp1":"-3.606.294.632","T7_3_Al_exp2":"-3.434.806.824","T7_3_Al_exp3":"-2.655.748.367"},{"Metabolite":"AlkanylTG (O-16:0_16:0_16:0)b","T0_1_Al_exp1":"-1.792.669.296","T0_1_Al_exp2":"-196.062.088","T0_1_Al_exp3":"-1.127.429.962","T0_2_Al_exp1":"-1.869.838.715","T0_2_Al_exp2":"-20.082.798","T0_2_Al_exp3":"-0.629655838","T0_3_Al_exp1":"-1.346.054.077","T0_3_Al_exp2":"-1.871.595.383","T0_3_Al_exp3":"-1.059.263.229","T1_1_Al_exp1":"-3.461.076.736","T1_1_Al_exp2":"-3.642.631.531","T1_1_Al_exp3":"-2.002.569.199","T1_2_Al_exp1":"-3.578.020.096","T1_2_Al_exp2":"-3.880.411.148","T1_2_Al_exp3":"-2.090.801.239","T1_3_Al_exp1":"-3.624.389.648","T1_3_Al_exp2":"-3.443.805.695","T1_3_Al_exp3":"-2.079.963.684","T7_1_Al_exp1":"-2.862.016.678","T7_1_Al_exp2":"-2.618.400.574","T7_1_Al_exp3":"-1.772.233.963","T7_2_Al_exp1":"-2.874.864.578","T7_2_Al_exp2":"-2.801.523.209","T7_2_Al_exp3":"-1.457.883.835","T7_3_Al_exp1":"-2.626.581.192","T7_3_Al_exp2":"-2.601.501.465","T7_3_Al_exp3":"-1.515.083.313"},{"Metabolite":"AlkanylTG (O-50:0)","T0_1_Al_exp1":"-2.147.609.711","T0_1_Al_exp2":"-2.199.279.785","T0_1_Al_exp3":"-1.542.173.386","T0_2_Al_exp1":"-2.244.890.213","T0_2_Al_exp2":"-2.328.195.572","T0_2_Al_exp3":"-1.063.688.278","T0_3_Al_exp1":"-1.653.644.562","T0_3_Al_exp2":"-2.112.596.512","T0_3_Al_exp3":"-1.522.550.583","T1_1_Al_exp1":"-3.332.706.451","T1_1_Al_exp2":"-3.524.103.165","T1_1_Al_exp3":"-1.938.680.649","T1_2_Al_exp1":"-3.753.992.081","T1_2_Al_exp2":"-3.830.318.451","T1_2_Al_exp3":"-2.073.284.149","T1_3_Al_exp1":"-362.279.129","T1_3_Al_exp2":"-337.833.786","T1_3_Al_exp3":"-2.109.689.713","T7_1_Al_exp1":"-3.321.098.328","T7_1_Al_exp2":"-3.092.218.399","T7_1_Al_exp3":"-2.530.918.121","T7_2_Al_exp1":"-3.374.235.153","T7_2_Al_exp2":"-3.295.557.022","T7_2_Al_exp3":"-2.184.339.523","T7_3_Al_exp1":"-3.195.768.356","T7_3_Al_exp2":"-3.119.903.564","T7_3_Al_exp3":"-2.334.413.528"},{"Metabolite":"AlkenylTG (P-20:0_18:1_18:1)","T0_1_Al_exp1":"-2.983.198.166","T0_1_Al_exp2":"-3.120.210.648","T0_1_Al_exp3":"-1.500.640.869","T0_2_Al_exp1":"-2.332.893.372","T0_2_Al_exp2":"-2.749.042.511","T0_2_Al_exp3":"-1.786.819.458","T0_3_Al_exp1":"-2.198.011.398","T0_3_Al_exp2":"-2.705.989.838","T0_3_Al_exp3":"-1.729.408.264","T1_1_Al_exp1":"-3.486.110.687","T1_1_Al_exp2":"-4.634.326.935","T1_1_Al_exp3":"-228.194.046","T1_2_Al_exp1":"-548.481.369","T1_2_Al_exp2":"-39.030.056","T1_2_Al_exp3":"-2.760.149.002","T1_3_Al_exp1":"-3.576.957.703","T1_3_Al_exp2":"-3.739.032.745","T1_3_Al_exp3":"-2.649.913.788","T7_1_Al_exp1":"-3.081.274.033","T7_1_Al_exp2":"-3.041.566.849","T7_1_Al_exp3":"-1.837.453.842","T7_2_Al_exp1":"-3.158.309.937","T7_2_Al_exp2":"-3.759.895.325","T7_2_Al_exp3":"-2.016.950.607","T7_3_Al_exp1":"-3.829.401.016","T7_3_Al_exp2":"-3.007.297.516","T7_3_Al_exp3":"-2.136.550.903"},{"Metabolite":"AlkenylTG (P-48:0)","T0_1_Al_exp1":"-0.441392899","T0_1_Al_exp2":"-0.575860977","T0_1_Al_exp3":"0.234165192","T0_2_Al_exp1":"-0.397947311","T0_2_Al_exp2":"-0.68778801","T0_2_Al_exp3":"0.54457283","T0_3_Al_exp1":"-0.278942108","T0_3_Al_exp2":"-0.464929581","T0_3_Al_exp3":"0.156164169","T1_1_Al_exp1":"-1.836.387.634","T1_1_Al_exp2":"-1.974.943.161","T1_1_Al_exp3":"-0.588478088","T1_2_Al_exp1":"-202.623.558","T1_2_Al_exp2":"-2.114.631.653","T1_2_Al_exp3":"-0.764310837","T1_3_Al_exp1":"-1.970.993.042","T1_3_Al_exp2":"-1.706.624.985","T1_3_Al_exp3":"-0.763669968","T7_1_Al_exp1":"-1.088.159.561","T7_1_Al_exp2":"-1.008.749.008","T7_1_Al_exp3":"-0.167715073","T7_2_Al_exp1":"-1.218.610.764","T7_2_Al_exp2":"-1.080.234.528","T7_2_Al_exp3":"-0.072544098","T7_3_Al_exp1":"-1.039.890.289","T7_3_Al_exp2":"-100.569.725","T7_3_Al_exp3":"-0.032489777"},{"Metabolite":"AlkenylTG (P-48:1)","T0_1_Al_exp1":"-2.344.911.575","T0_1_Al_exp2":"-2.489.580.154","T0_1_Al_exp3":"-1.928.674.698","T0_2_Al_exp1":"-2.519.117.355","T0_2_Al_exp2":"-2.746.046.066","T0_2_Al_exp3":"-17.289.505","T0_3_Al_exp1":"-2.535.181.046","T0_3_Al_exp2":"-2.536.430.359","T0_3_Al_exp3":"-1.849.592.209","T1_1_Al_exp1":"-3.115.800.858","T1_1_Al_exp2":"-2.806.148.529","T1_1_Al_exp3":"-2.204.454.422","T1_2_Al_exp1":"-3.408.956.528","T1_2_Al_exp2":"-3.047.149.658","T1_2_Al_exp3":"-2.266.548.157","T1_3_Al_exp1":"-3.196.670.532","T1_3_Al_exp2":"-265.243.721","T1_3_Al_exp3":"-2.259.649.277","T7_1_Al_exp1":"-2.659.702.301","T7_1_Al_exp2":"-2.407.966.614","T7_1_Al_exp3":"-1.871.725.082","T7_2_Al_exp1":"-2.813.623.428","T7_2_Al_exp2":"-2.812.259.674","T7_2_Al_exp3":"-1.966.180.801","T7_3_Al_exp1":"-3.005.123.138","T7_3_Al_exp2":"-2.493.371.964","T7_3_Al_exp3":"-1.918.828.964"},{"Metabolite":"AlkenylTG (P-50:0)","T0_1_Al_exp1":"0.248168945","T0_1_Al_exp2":"0.234294891","T0_1_Al_exp3":"1.019.048.691","T0_2_Al_exp1":"0.368061066","T0_2_Al_exp2":"0.207529068","T0_2_Al_exp3":"1.352.319.717","T0_3_Al_exp1":"-0.03339386","T0_3_Al_exp2":"0.348577499","T0_3_Al_exp3":"1.139.709.473","T1_1_Al_exp1":"-1.024.824.142","T1_1_Al_exp2":"-117.716.217","T1_1_Al_exp3":"0.322025299","T1_2_Al_exp1":"-1.178.056.717","T1_2_Al_exp2":"-1.468.803.406","T1_2_Al_exp3":"0.165456772","T1_3_Al_exp1":"-1.300.788.879","T1_3_Al_exp2":"-0.980751038","T1_3_Al_exp3":"0.207511902","T7_1_Al_exp1":"-0.782999039","T7_1_Al_exp2":"-0.340463638","T7_1_Al_exp3":"0.453243256","T7_2_Al_exp1":"-0.497629166","T7_2_Al_exp2":"-1.238.325.119","T7_2_Al_exp3":"0.624019623","T7_3_Al_exp1":"-0.344232559","T7_3_Al_exp2":"-0.362955093","T7_3_Al_exp3":"0.707006454"},{"Metabolite":"AlkenylTG 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