{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001968","ANALYSIS_ID":"AN003207","VERSION":"1","CREATED_ON":"September 27, 2021, 3:33 am"},

"PROJECT":{"PROJECT_TITLE":"Analytical methodology for a metabolome atlas of goat’s plas-ma, milk and feces using 1H-NMR and UHPLC-HRMS","PROJECT_SUMMARY":"Metabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factor that can also alter animal integrity and welfare. Some studies have al-ready investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (Nu-clear Magnetic Resonance or Mass Spectrometry). Only few studies with multi-platform ap-proaches are also used with a single or a multi type of sample, but they mainly consider dairy cows metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabol-ic atlas of goat plasma, milk and feces, based on healthy animals. Our study describes a Standard Operating Procedure for three goat matrices: blood plasma, milk, and feces using multiple plat-forms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample prep-aration procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.","INSTITUTE":"INSERM","LAST_NAME":"Martias","FIRST_NAME":"Cecile","ADDRESS":"10 Boulevard tonnelé, Tours, Indre et Loire, 37000, France","EMAIL":"cecile.martias@univ-tours.fr","PHONE":"0247366351"},

"STUDY":{"STUDY_TITLE":"Analytical methodology for a metabolome atlas of goat’s plas-ma, milk and feces using 1H-NMR and UHPLC-HRMS","STUDY_SUMMARY":"Metabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factor that can also alter animal integrity and welfare. Some studies have al-ready investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (Nu-clear Magnetic Resonance or Mass Spectrometry). Only few studies with multi-platform ap-proaches are also used with a single or a multi type of sample, but they mainly consider dairy cows metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabol-ic atlas of goat plasma, milk and feces, based on healthy animals. Our study describes a Standard Operating Procedure for three goat matrices: blood plasma, milk, and feces using multiple plat-forms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample prep-aration procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.","INSTITUTE":"INSERM","LAST_NAME":"Martias","FIRST_NAME":"Cecile","ADDRESS":"10 Boulevard tonnelé, Tours, Indre et Loire, 37000, France","EMAIL":"cecile.martias@univ-tours.fr","PHONE":"0247366351"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Goat"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"P_200_P1_1",
"Factors":{"Protocole":"P1"},
"Additional sample data":{"RAW_FILE_NAME":"P_200_P1_1"}
},
{
"Subject ID":"-",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P1_2"}
},
{
"Subject ID":"-",
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"Factors":{"Protocole":"P1"},
"Additional sample data":{"RAW_FILE_NAME":"P_200_P1_3"}
},
{
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"Factors":{"Protocole":"P1"},
"Additional sample data":{"RAW_FILE_NAME":"P_200_P1_4"}
},
{
"Subject ID":"-",
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"Factors":{"Protocole":"P1"},
"Additional sample data":{"RAW_FILE_NAME":"P_200_P1_5"}
},
{
"Subject ID":"-",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P1_6"}
},
{
"Subject ID":"-",
"Sample ID":"P_200_P1_7",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P1_7"}
},
{
"Subject ID":"-",
"Sample ID":"P_200_P1_8",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P1_8"}
},
{
"Subject ID":"-",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P1_9"}
},
{
"Subject ID":"-",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P1_10"}
},
{
"Subject ID":"-",
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"Factors":{"Protocole":"P2"},
"Additional sample data":{"RAW_FILE_NAME":"P_200_P2_1"}
},
{
"Subject ID":"-",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P2_2"}
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{
"Subject ID":"-",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P2_3"}
},
{
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P2_4"}
},
{
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{
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{
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{
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P2_8"}
},
{
"Subject ID":"-",
"Sample ID":"P_200_P2_9",
"Factors":{"Protocole":"p2"},
"Additional sample data":{"RAW_FILE_NAME":"P_200_P2_9"}
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{
"Subject ID":"-",
"Sample ID":"P_200_P2_10",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P2_10"}
},
{
"Subject ID":"-",
"Sample ID":"P_200_P4_1",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P4_1"}
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{
"Subject ID":"-",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P4_2"}
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{
"Subject ID":"-",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P4_3"}
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{
"Subject ID":"-",
"Sample ID":"P_200_P4_4",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P4_4"}
},
{
"Subject ID":"-",
"Sample ID":"P_200_P4_5",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P4_5"}
},
{
"Subject ID":"-",
"Sample ID":"P_200_P4_6",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P4_6"}
},
{
"Subject ID":"-",
"Sample ID":"P_200_P4_7",
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"Additional sample data":{"RAW_FILE_NAME":"P_200_P4_7"}
},
{
"Subject ID":"-",
"Sample ID":"P_200_P4_8",
"Factors":{"Protocole":"P4"},
"Additional sample data":{"RAW_FILE_NAME":"P_200_P4_8"}
},
{
"Subject ID":"-",
"Sample ID":"P_200_P4_9",
"Factors":{"Protocole":"P4"},
"Additional sample data":{"RAW_FILE_NAME":"P_200_P4_9"}
},
{
"Subject ID":"-",
"Sample ID":"P_200_P4_10",
"Factors":{"Protocole":"P4"},
"Additional sample data":{"RAW_FILE_NAME":"P_200_P4_10"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","SAMPLE_TYPE":"Blood (plasma)"},

"TREATMENT":{"TREATMENT_SUMMARY":"P1 : Blood:MeOH:CHCl3 1:1:1 P2: Blood:MeOH:CHCl3 1:2.5:2.5 P4: Blood:MeOH:CHCl3 1:8"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Phenomenex","COLUMN_NAME":"Phenomenex Kinetex XB-C18 (1.7µm 100 A 150x 2.1mm)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"-"},

"MS_METABOLITE_DATA":{
"Units":"SI",

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}

}