#METABOLOMICS WORKBENCH ssah9_20220126_062026 DATATRACK_ID:3054 STUDY_ID:ST002067 ANALYSIS_ID:AN003367 PROJECT_ID:PR001309 VERSION 1 CREATED_ON January 26, 2022, 8:51 am #PROJECT PR:PROJECT_TITLE Time-Resolved Metabolomics of a Mouse Model of Ovarian High-Grade Serous PR:PROJECT_TITLE Carcinoma . PR:PROJECT_TYPE Untargeted serum metabolomics and mass spectrometry imaging PR:PROJECT_SUMMARY The dismally-low survival rate of ovarian cancer patients diagnosed with PR:PROJECT_SUMMARY high-grade serous carcinoma (HGSC) emphasizes the lack of effective screening PR:PROJECT_SUMMARY strategies. One major obstacle is the limited knowledge of the underlying PR:PROJECT_SUMMARY mechanisms of HGSC pathogenesis at very early stages. Here, we present the first PR:PROJECT_SUMMARY 10-month time-resolved serum metabolic profile of a triple mutant (TKO) HGSC PR:PROJECT_SUMMARY mouse model, along with the spatial lipidome profile of its entire reproductive PR:PROJECT_SUMMARY system. A high-coverage liquid chromatography mass spectrometry-based PR:PROJECT_SUMMARY metabolomics approach was applied to longitudinally-collected serum samples from PR:PROJECT_SUMMARY both TKO and TKO control mice, tracking metabolome and lipidome changes from PR:PROJECT_SUMMARY disease onset until death. Spatial lipid distributions within the reproductive PR:PROJECT_SUMMARY system were also mapped via ultrahigh-resolution matrix-assisted laser PR:PROJECT_SUMMARY desorption/ionization (MALDI) mass spectrometry, and compared with serum lipid PR:PROJECT_SUMMARY profiles for various lipid classes. Altogether, our results show that the PR:PROJECT_SUMMARY remodeling of lipid and fatty acid metabolism, amino acid biosynthesis, TCA PR:PROJECT_SUMMARY cycle and ovarian steroidogenesis are critical components of HGSC onset and PR:PROJECT_SUMMARY development. These metabolic alterations are accompanied by changes in energy PR:PROJECT_SUMMARY metabolism, mitochondrial and peroxisomal function, redox homeostasis, and PR:PROJECT_SUMMARY inflammatory response, collectively supporting tumorigenesis. PR:INSTITUTE Georgia Institute of Technology PR:DEPARTMENT Chemistry and Biochemistry PR:LAST_NAME Sah PR:FIRST_NAME Samyukta PR:ADDRESS 901 Atlantic Dr NE, Atlanta, GA, 30332, USA PR:EMAIL ssah9@gatech.edu PR:PHONE 574-678-0124 #STUDY ST:STUDY_TITLE Time-Resolved Metabolomics of a Mouse Model of Ovarian High-Grade Serous ST:STUDY_TITLE Carcinoma (LC-MS) ST:STUDY_SUMMARY The dismally-low survival rate of ovarian cancer patients diagnosed with ST:STUDY_SUMMARY high-grade serous carcinoma (HGSC) emphasizes the lack of effective screening ST:STUDY_SUMMARY strategies. One major obstacle is the limited knowledge of the underlying ST:STUDY_SUMMARY mechanisms of HGSC pathogenesis at very early stages. Here, we present the first ST:STUDY_SUMMARY 10-month time-resolved serum metabolic profile of a triple mutant (TKO) HGSC ST:STUDY_SUMMARY mouse model, along with the spatial lipidome profile of its entire reproductive ST:STUDY_SUMMARY system. A high-coverage liquid chromatography mass spectrometry-based ST:STUDY_SUMMARY metabolomics approach was applied to longitudinally-collected serum samples from ST:STUDY_SUMMARY both TKO and TKO control mice, tracking metabolome and lipidome changes from ST:STUDY_SUMMARY disease onset until death. Spatial lipid distributions within the reproductive ST:STUDY_SUMMARY system were also mapped via ultrahigh-resolution matrix-assisted laser ST:STUDY_SUMMARY desorption/ionization (MALDI) mass spectrometry, and compared with serum lipid ST:STUDY_SUMMARY profiles for various lipid classes. Altogether, our results show that the ST:STUDY_SUMMARY remodeling of lipid and fatty acid metabolism, amino acid biosynthesis, TCA ST:STUDY_SUMMARY cycle and ovarian steroidogenesis are critical components of HGSC onset and ST:STUDY_SUMMARY development. These metabolic alterations are accompanied by changes in energy ST:STUDY_SUMMARY metabolism, mitochondrial and peroxisomal function, redox homeostasis, and ST:STUDY_SUMMARY inflammatory response, collectively supporting tumorigenesis. ST:INSTITUTE Georgia Institute of Technology ST:LAST_NAME Sah ST:FIRST_NAME Samyukta ST:ADDRESS 901 Atlantic Dr NE, Atlanta, GA, 30332, USA ST:EMAIL ssah9@gatech.edu ST:PHONE 574-678-0124 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENDER Female #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - T1_1 Factor:TKO RAW_FILE_NAME=TKO_7320_1 SUBJECT_SAMPLE_FACTORS - T1_2 Factor:TKO RAW_FILE_NAME=TKO_7320_2 SUBJECT_SAMPLE_FACTORS - T1_3 Factor:TKO RAW_FILE_NAME=TKO_7320_3 SUBJECT_SAMPLE_FACTORS - T1_4 Factor:TKO RAW_FILE_NAME=TKO_7320_4 SUBJECT_SAMPLE_FACTORS - T1_5 Factor:TKO RAW_FILE_NAME=TKO_7320_5 SUBJECT_SAMPLE_FACTORS - T1_6 Factor:TKO RAW_FILE_NAME=TKO_7320_6 SUBJECT_SAMPLE_FACTORS - T1_7 Factor:TKO RAW_FILE_NAME=TKO_7320_7 SUBJECT_SAMPLE_FACTORS - T1_8 Factor:TKO RAW_FILE_NAME=TKO_7320_8 SUBJECT_SAMPLE_FACTORS - T1_9 Factor:TKO RAW_FILE_NAME=TKO_7320_9 SUBJECT_SAMPLE_FACTORS - T1_10 Factor:TKO RAW_FILE_NAME=TKO_7320_10 SUBJECT_SAMPLE_FACTORS - T1_11 Factor:TKO RAW_FILE_NAME=TKO_7320_11 SUBJECT_SAMPLE_FACTORS - T1_12 Factor:TKO RAW_FILE_NAME=TKO_7320_12 SUBJECT_SAMPLE_FACTORS - T1_13 Factor:TKO RAW_FILE_NAME=TKO_7320_13 SUBJECT_SAMPLE_FACTORS - T4_1 Factor:TKO RAW_FILE_NAME=TKO_7390_1 SUBJECT_SAMPLE_FACTORS - T4_2 Factor:TKO RAW_FILE_NAME=TKO_7390_2 SUBJECT_SAMPLE_FACTORS - T4_3 Factor:TKO RAW_FILE_NAME=TKO_7390_3 SUBJECT_SAMPLE_FACTORS - T4_4 Factor:TKO RAW_FILE_NAME=TKO_7390_4 SUBJECT_SAMPLE_FACTORS - T4_5 Factor:TKO RAW_FILE_NAME=TKO_7390_5 SUBJECT_SAMPLE_FACTORS - T4_6 Factor:TKO RAW_FILE_NAME=TKO_7390_6 SUBJECT_SAMPLE_FACTORS - T4_7 Factor:TKO RAW_FILE_NAME=TKO_7390_7 SUBJECT_SAMPLE_FACTORS - T4_8 Factor:TKO RAW_FILE_NAME=TKO_7390_8 SUBJECT_SAMPLE_FACTORS - T4_9 Factor:TKO RAW_FILE_NAME=TKO_7390_9 SUBJECT_SAMPLE_FACTORS - T5_1 Factor:TKO RAW_FILE_NAME=TKO_7453_1 SUBJECT_SAMPLE_FACTORS - T5_2 Factor:TKO RAW_FILE_NAME=TKO_7453_2 SUBJECT_SAMPLE_FACTORS - T5_3 Factor:TKO RAW_FILE_NAME=TKO_7453_3 SUBJECT_SAMPLE_FACTORS - T5_4 Factor:TKO RAW_FILE_NAME=TKO_7453_4 SUBJECT_SAMPLE_FACTORS - T5_5 Factor:TKO RAW_FILE_NAME=TKO_7453_5 SUBJECT_SAMPLE_FACTORS - T5_6 Factor:TKO RAW_FILE_NAME=TKO_7453_6 SUBJECT_SAMPLE_FACTORS - T5_7 Factor:TKO RAW_FILE_NAME=TKO_7453_7 SUBJECT_SAMPLE_FACTORS - T5_8 Factor:TKO RAW_FILE_NAME=TKO_7453_8 SUBJECT_SAMPLE_FACTORS - T5_9 Factor:TKO RAW_FILE_NAME=TKO_7453_9 SUBJECT_SAMPLE_FACTORS - T7_1 Factor:TKO RAW_FILE_NAME=TKO_8097_1 SUBJECT_SAMPLE_FACTORS - T7_2 Factor:TKO RAW_FILE_NAME=TKO_8097_2 SUBJECT_SAMPLE_FACTORS - T7_3 Factor:TKO RAW_FILE_NAME=TKO_8097_3 SUBJECT_SAMPLE_FACTORS - T7_4 Factor:TKO RAW_FILE_NAME=TKO_8097_4 SUBJECT_SAMPLE_FACTORS - T7_5 Factor:TKO RAW_FILE_NAME=TKO_8097_5 SUBJECT_SAMPLE_FACTORS - T7_6 Factor:TKO RAW_FILE_NAME=TKO_8097_6 SUBJECT_SAMPLE_FACTORS - T7_7 Factor:TKO RAW_FILE_NAME=TKO_8097_7 SUBJECT_SAMPLE_FACTORS - T7_8 Factor:TKO RAW_FILE_NAME=TKO_8097_8 SUBJECT_SAMPLE_FACTORS - T7_9 Factor:TKO RAW_FILE_NAME=TKO_8097_9 SUBJECT_SAMPLE_FACTORS - T8_1 Factor:TKO RAW_FILE_NAME=TKO_8098_1 SUBJECT_SAMPLE_FACTORS - T8_2 Factor:TKO RAW_FILE_NAME=TKO_8098_2 SUBJECT_SAMPLE_FACTORS - T8_3 Factor:TKO RAW_FILE_NAME=TKO_8098_3 SUBJECT_SAMPLE_FACTORS - T8_4 Factor:TKO RAW_FILE_NAME=TKO_8098_4 SUBJECT_SAMPLE_FACTORS - T8_5 Factor:TKO RAW_FILE_NAME=TKO_8098_5 SUBJECT_SAMPLE_FACTORS - T8_6 Factor:TKO RAW_FILE_NAME=TKO_8098_6 SUBJECT_SAMPLE_FACTORS - T8_7 Factor:TKO RAW_FILE_NAME=TKO_8098_7 SUBJECT_SAMPLE_FACTORS - T8_8 Factor:TKO RAW_FILE_NAME=TKO_8098_8 SUBJECT_SAMPLE_FACTORS - T9_1 Factor:TKO RAW_FILE_NAME=TKO_8100_1 SUBJECT_SAMPLE_FACTORS - T9_2 Factor:TKO RAW_FILE_NAME=TKO_8100_2 SUBJECT_SAMPLE_FACTORS - T9_3 Factor:TKO RAW_FILE_NAME=TKO_8100_3 SUBJECT_SAMPLE_FACTORS - T9_4 Factor:TKO RAW_FILE_NAME=TKO_8100_4 SUBJECT_SAMPLE_FACTORS - T9_5 Factor:TKO RAW_FILE_NAME=TKO_8100_5 SUBJECT_SAMPLE_FACTORS - T9_6 Factor:TKO RAW_FILE_NAME=TKO_8100_6 SUBJECT_SAMPLE_FACTORS - T9_7 Factor:TKO RAW_FILE_NAME=TKO_8100_7 SUBJECT_SAMPLE_FACTORS - T9_8 Factor:TKO RAW_FILE_NAME=TKO_8100_8 SUBJECT_SAMPLE_FACTORS - T9_9 Factor:TKO RAW_FILE_NAME=TKO_8100_9 SUBJECT_SAMPLE_FACTORS - T10_1 Factor:TKO RAW_FILE_NAME=TKO_8104_1 SUBJECT_SAMPLE_FACTORS - T10_2 Factor:TKO RAW_FILE_NAME=TKO_8104_2 SUBJECT_SAMPLE_FACTORS - T10_3 Factor:TKO RAW_FILE_NAME=TKO_8104_3 SUBJECT_SAMPLE_FACTORS - T10_4 Factor:TKO RAW_FILE_NAME=TKO_8104_4 SUBJECT_SAMPLE_FACTORS - T10_5 Factor:TKO RAW_FILE_NAME=TKO_8104_5 SUBJECT_SAMPLE_FACTORS - T10_6 Factor:TKO RAW_FILE_NAME=TKO_8104_6 SUBJECT_SAMPLE_FACTORS - T10_7 Factor:TKO RAW_FILE_NAME=TKO_8104_7 SUBJECT_SAMPLE_FACTORS - T10_8 Factor:TKO RAW_FILE_NAME=TKO_8104_8 SUBJECT_SAMPLE_FACTORS - T10_9 Factor:TKO RAW_FILE_NAME=TKO_8104_9 SUBJECT_SAMPLE_FACTORS - T10_10 Factor:TKO RAW_FILE_NAME=TKO_8104_10 SUBJECT_SAMPLE_FACTORS - T10_11 Factor:TKO RAW_FILE_NAME=TKO_8104_11 SUBJECT_SAMPLE_FACTORS - T11_1 Factor:TKO RAW_FILE_NAME=TKO_8256_1 SUBJECT_SAMPLE_FACTORS - T11_2 Factor:TKO RAW_FILE_NAME=TKO_8256_2 SUBJECT_SAMPLE_FACTORS - T11_3 Factor:TKO RAW_FILE_NAME=TKO_8256_3 SUBJECT_SAMPLE_FACTORS - T11_4 Factor:TKO RAW_FILE_NAME=TKO_8256_4 SUBJECT_SAMPLE_FACTORS - T11_5 Factor:TKO RAW_FILE_NAME=TKO_8256_5 SUBJECT_SAMPLE_FACTORS - T11_6 Factor:TKO RAW_FILE_NAME=TKO_8256_6 SUBJECT_SAMPLE_FACTORS - T11_7 Factor:TKO RAW_FILE_NAME=TKO_8256_7 SUBJECT_SAMPLE_FACTORS - T11_8 Factor:TKO RAW_FILE_NAME=TKO_8256_8 SUBJECT_SAMPLE_FACTORS - T11_9 Factor:TKO RAW_FILE_NAME=TKO_8256_9 SUBJECT_SAMPLE_FACTORS - T11_10 Factor:TKO RAW_FILE_NAME=TKO_8256_10 SUBJECT_SAMPLE_FACTORS - T11_11 Factor:TKO RAW_FILE_NAME=TKO_8256_11 SUBJECT_SAMPLE_FACTORS - T11_12 Factor:TKO RAW_FILE_NAME=TKO_8256_12 SUBJECT_SAMPLE_FACTORS - T11_13 Factor:TKO RAW_FILE_NAME=TKO_8256_13 SUBJECT_SAMPLE_FACTORS - T11_14 Factor:TKO RAW_FILE_NAME=TKO_8256_14 SUBJECT_SAMPLE_FACTORS - T11_15 Factor:TKO RAW_FILE_NAME=TKO_8256_15 SUBJECT_SAMPLE_FACTORS - T12_1 Factor:TKO RAW_FILE_NAME=TKO_8288_1 SUBJECT_SAMPLE_FACTORS - T12_2 Factor:TKO RAW_FILE_NAME=TKO_8288_2 SUBJECT_SAMPLE_FACTORS - T12_3 Factor:TKO RAW_FILE_NAME=TKO_8288_3 SUBJECT_SAMPLE_FACTORS - T12_4 Factor:TKO RAW_FILE_NAME=TKO_8288_4 SUBJECT_SAMPLE_FACTORS - T12_5 Factor:TKO RAW_FILE_NAME=TKO_8288_5 SUBJECT_SAMPLE_FACTORS - T12_6 Factor:TKO RAW_FILE_NAME=TKO_8288_6 SUBJECT_SAMPLE_FACTORS - T12_7 Factor:TKO RAW_FILE_NAME=TKO_8288_7 SUBJECT_SAMPLE_FACTORS - T12_8 Factor:TKO RAW_FILE_NAME=TKO_8288_8 SUBJECT_SAMPLE_FACTORS - T12_9 Factor:TKO RAW_FILE_NAME=TKO_8288_9 SUBJECT_SAMPLE_FACTORS - T12_10 Factor:TKO RAW_FILE_NAME=TKO_8288_10 SUBJECT_SAMPLE_FACTORS - T13_1 Factor:TKO RAW_FILE_NAME=TKO_8465_1 SUBJECT_SAMPLE_FACTORS - T13_2 Factor:TKO RAW_FILE_NAME=TKO_8465_2 SUBJECT_SAMPLE_FACTORS - T13_3 Factor:TKO RAW_FILE_NAME=TKO_8465_3 SUBJECT_SAMPLE_FACTORS - T13_4 Factor:TKO RAW_FILE_NAME=TKO_8465_4 SUBJECT_SAMPLE_FACTORS - T13_5 Factor:TKO RAW_FILE_NAME=TKO_8465_5 SUBJECT_SAMPLE_FACTORS - T13_6 Factor:TKO RAW_FILE_NAME=TKO_8465_6 SUBJECT_SAMPLE_FACTORS - T13_7 Factor:TKO RAW_FILE_NAME=TKO_8465_7 SUBJECT_SAMPLE_FACTORS - T13_8 Factor:TKO RAW_FILE_NAME=TKO_8465_8 SUBJECT_SAMPLE_FACTORS - T13_9 Factor:TKO RAW_FILE_NAME=TKO_8465_9 SUBJECT_SAMPLE_FACTORS - T14_1 Factor:TKO RAW_FILE_NAME=TKO_8470_1 SUBJECT_SAMPLE_FACTORS - T14_2 Factor:TKO RAW_FILE_NAME=TKO_8470_2 SUBJECT_SAMPLE_FACTORS - T14_3 Factor:TKO RAW_FILE_NAME=TKO_8470_3 SUBJECT_SAMPLE_FACTORS - T14_4 Factor:TKO RAW_FILE_NAME=TKO_8470_4 SUBJECT_SAMPLE_FACTORS - T14_5 Factor:TKO RAW_FILE_NAME=TKO_8470_5 SUBJECT_SAMPLE_FACTORS - T14_6 Factor:TKO RAW_FILE_NAME=TKO_8470_6 SUBJECT_SAMPLE_FACTORS - T14_7 Factor:TKO RAW_FILE_NAME=TKO_8470_7 SUBJECT_SAMPLE_FACTORS - T14_8 Factor:TKO RAW_FILE_NAME=TKO_8470_8 SUBJECT_SAMPLE_FACTORS - T15_1 Factor:TKO RAW_FILE_NAME=TKO_8472_1 SUBJECT_SAMPLE_FACTORS - T15_2 Factor:TKO RAW_FILE_NAME=TKO_8472_2 SUBJECT_SAMPLE_FACTORS - T15_3 Factor:TKO RAW_FILE_NAME=TKO_8472_3 SUBJECT_SAMPLE_FACTORS - T15_4 Factor:TKO RAW_FILE_NAME=TKO_8472_4 SUBJECT_SAMPLE_FACTORS - T15_5 Factor:TKO RAW_FILE_NAME=TKO_8472_5 SUBJECT_SAMPLE_FACTORS - T15_6 Factor:TKO RAW_FILE_NAME=TKO_8472_6 SUBJECT_SAMPLE_FACTORS - T15_7 Factor:TKO RAW_FILE_NAME=TKO_8472_7 SUBJECT_SAMPLE_FACTORS - T15_8 Factor:TKO RAW_FILE_NAME=TKO_8472_8 SUBJECT_SAMPLE_FACTORS - T15_9 Factor:TKO RAW_FILE_NAME=TKO_8472_9 SUBJECT_SAMPLE_FACTORS - T15_10 Factor:TKO RAW_FILE_NAME=TKO_8472_10 SUBJECT_SAMPLE_FACTORS - T15_11 Factor:TKO RAW_FILE_NAME=TKO_8472_11 SUBJECT_SAMPLE_FACTORS - T15_12 Factor:TKO RAW_FILE_NAME=TKO_8472_12 SUBJECT_SAMPLE_FACTORS - T17_1 Factor:TKO RAW_FILE_NAME=TKO_8747_1 SUBJECT_SAMPLE_FACTORS - T17_2 Factor:TKO RAW_FILE_NAME=TKO_8747_2 SUBJECT_SAMPLE_FACTORS - T17_3 Factor:TKO RAW_FILE_NAME=TKO_8747_3 SUBJECT_SAMPLE_FACTORS - T17_4 Factor:TKO RAW_FILE_NAME=TKO_8747_4 SUBJECT_SAMPLE_FACTORS - T17_5 Factor:TKO RAW_FILE_NAME=TKO_8747_5 SUBJECT_SAMPLE_FACTORS - T17_6 Factor:TKO RAW_FILE_NAME=TKO_8747_6 SUBJECT_SAMPLE_FACTORS - T17_7 Factor:TKO RAW_FILE_NAME=TKO_8747_7 SUBJECT_SAMPLE_FACTORS - T17_8 Factor:TKO RAW_FILE_NAME=TKO_8747_8 SUBJECT_SAMPLE_FACTORS - T17_9 Factor:TKO RAW_FILE_NAME=TKO_8747_9 SUBJECT_SAMPLE_FACTORS - T17_10 Factor:TKO RAW_FILE_NAME=TKO_8747_10 SUBJECT_SAMPLE_FACTORS - T17_11 Factor:TKO RAW_FILE_NAME=TKO_8747_11 SUBJECT_SAMPLE_FACTORS - T17_12 Factor:TKO RAW_FILE_NAME=TKO_8747_12 SUBJECT_SAMPLE_FACTORS - T17_13 Factor:TKO RAW_FILE_NAME=TKO_8747_13 SUBJECT_SAMPLE_FACTORS - T3_1 Factor:TKO RAW_FILE_NAME=TKO_7245_1 SUBJECT_SAMPLE_FACTORS - T3_2 Factor:TKO RAW_FILE_NAME=TKO_7245_2 SUBJECT_SAMPLE_FACTORS - T3_3 Factor:TKO RAW_FILE_NAME=TKO_7245_3 SUBJECT_SAMPLE_FACTORS - T3_4 Factor:TKO RAW_FILE_NAME=TKO_7245_4 SUBJECT_SAMPLE_FACTORS - T3_5 Factor:TKO RAW_FILE_NAME=TKO_7245_5 SUBJECT_SAMPLE_FACTORS - T3_6 Factor:TKO RAW_FILE_NAME=TKO_7245_6 SUBJECT_SAMPLE_FACTORS - T3_7 Factor:TKO RAW_FILE_NAME=TKO_7245_7 SUBJECT_SAMPLE_FACTORS - T3_8 Factor:TKO RAW_FILE_NAME=TKO_7245_8 SUBJECT_SAMPLE_FACTORS - T3_9 Factor:TKO RAW_FILE_NAME=TKO_7245_9 SUBJECT_SAMPLE_FACTORS - T3_10 Factor:TKO RAW_FILE_NAME=TKO_7245_10 SUBJECT_SAMPLE_FACTORS - T6_1 Factor:TKO RAW_FILE_NAME=TKO_7529_1 SUBJECT_SAMPLE_FACTORS - T6_2 Factor:TKO RAW_FILE_NAME=TKO_7529_2 SUBJECT_SAMPLE_FACTORS - T6_3 Factor:TKO RAW_FILE_NAME=TKO_7529_3 SUBJECT_SAMPLE_FACTORS - T6_4 Factor:TKO RAW_FILE_NAME=TKO_7529_4 SUBJECT_SAMPLE_FACTORS - T6_5 Factor:TKO RAW_FILE_NAME=TKO_7529_5 SUBJECT_SAMPLE_FACTORS - T6_6 Factor:TKO RAW_FILE_NAME=TKO_7529_6 SUBJECT_SAMPLE_FACTORS - T6_7 Factor:TKO RAW_FILE_NAME=TKO_7529_7 SUBJECT_SAMPLE_FACTORS - T6_8 Factor:TKO RAW_FILE_NAME=TKO_7529_8 SUBJECT_SAMPLE_FACTORS - T6_9 Factor:TKO RAW_FILE_NAME=TKO_7529_9 SUBJECT_SAMPLE_FACTORS - T6_10 Factor:TKO RAW_FILE_NAME=TKO_7529_10 SUBJECT_SAMPLE_FACTORS - C1_1 Factor:Control RAW_FILE_NAME=Ctrl_7244_1 SUBJECT_SAMPLE_FACTORS - C1_2 Factor:Control RAW_FILE_NAME=Ctrl_7244_2 SUBJECT_SAMPLE_FACTORS - C1_3 Factor:Control RAW_FILE_NAME=Ctrl_7244_3 SUBJECT_SAMPLE_FACTORS - C1_4 Factor:Control RAW_FILE_NAME=Ctrl_7244_4 SUBJECT_SAMPLE_FACTORS - C1_5 Factor:Control RAW_FILE_NAME=Ctrl_7244_5 SUBJECT_SAMPLE_FACTORS - C1_6 Factor:Control RAW_FILE_NAME=Ctrl_7244_6 SUBJECT_SAMPLE_FACTORS - C1_7 Factor:Control RAW_FILE_NAME=Ctrl_7244_7 SUBJECT_SAMPLE_FACTORS - C1_8 Factor:Control RAW_FILE_NAME=Ctrl_7244_8 SUBJECT_SAMPLE_FACTORS - C1_9 Factor:Control RAW_FILE_NAME=Ctrl_7244_9 SUBJECT_SAMPLE_FACTORS - C1_10 Factor:Control RAW_FILE_NAME=Ctrl_7244_10 SUBJECT_SAMPLE_FACTORS - C1_11 Factor:Control RAW_FILE_NAME=Ctrl_7244_11 SUBJECT_SAMPLE_FACTORS - C1_12 Factor:Control RAW_FILE_NAME=Ctrl_7244_12 SUBJECT_SAMPLE_FACTORS - C1_13 Factor:Control RAW_FILE_NAME=Ctrl_7244_13 SUBJECT_SAMPLE_FACTORS - C1_14 Factor:Control RAW_FILE_NAME=Ctrl_7244_14 SUBJECT_SAMPLE_FACTORS - C1_15 Factor:Control RAW_FILE_NAME=Ctrl_7244_15 SUBJECT_SAMPLE_FACTORS - C1_16 Factor:Control RAW_FILE_NAME=Ctrl_7244_16 SUBJECT_SAMPLE_FACTORS - C1_17 Factor:Control RAW_FILE_NAME=Ctrl_7244_17 SUBJECT_SAMPLE_FACTORS - C3_1 Factor:Control RAW_FILE_NAME=Ctrl_7527_1 SUBJECT_SAMPLE_FACTORS - C3_2 Factor:Control RAW_FILE_NAME=Ctrl_7527_2 SUBJECT_SAMPLE_FACTORS - C3_3 Factor:Control RAW_FILE_NAME=Ctrl_7527_3 SUBJECT_SAMPLE_FACTORS - C3_4 Factor:Control RAW_FILE_NAME=Ctrl_7527_4 SUBJECT_SAMPLE_FACTORS - C3_5 Factor:Control RAW_FILE_NAME=Ctrl_7527_5 SUBJECT_SAMPLE_FACTORS - C3_6 Factor:Control RAW_FILE_NAME=Ctrl_7527_6 SUBJECT_SAMPLE_FACTORS - C3_7 Factor:Control RAW_FILE_NAME=Ctrl_7527_7 SUBJECT_SAMPLE_FACTORS - C3_8 Factor:Control RAW_FILE_NAME=Ctrl_7527_8 SUBJECT_SAMPLE_FACTORS - C3_9 Factor:Control RAW_FILE_NAME=Ctrl_7527_9 SUBJECT_SAMPLE_FACTORS - C4_1 Factor:Control RAW_FILE_NAME=Ctrl_7531_1 SUBJECT_SAMPLE_FACTORS - C4_2 Factor:Control RAW_FILE_NAME=Ctrl_7531_2 SUBJECT_SAMPLE_FACTORS - C4_3 Factor:Control RAW_FILE_NAME=Ctrl_7531_3 SUBJECT_SAMPLE_FACTORS - C4_4 Factor:Control RAW_FILE_NAME=Ctrl_7531_4 SUBJECT_SAMPLE_FACTORS - C4_5 Factor:Control RAW_FILE_NAME=Ctrl_7531_5 SUBJECT_SAMPLE_FACTORS - C4_6 Factor:Control RAW_FILE_NAME=Ctrl_7531_6 SUBJECT_SAMPLE_FACTORS - C4_7 Factor:Control RAW_FILE_NAME=Ctrl_7531_7 SUBJECT_SAMPLE_FACTORS - C4_8 Factor:Control RAW_FILE_NAME=Ctrl_7531_8 SUBJECT_SAMPLE_FACTORS - C4_9 Factor:Control RAW_FILE_NAME=Ctrl_7531_9 SUBJECT_SAMPLE_FACTORS - C4_10 Factor:Control RAW_FILE_NAME=Ctrl_7531_10 SUBJECT_SAMPLE_FACTORS - C4_11 Factor:Control RAW_FILE_NAME=Ctrl_7531_11 SUBJECT_SAMPLE_FACTORS - C4_12 Factor:Control RAW_FILE_NAME=Ctrl_7531_12 SUBJECT_SAMPLE_FACTORS - C4_13 Factor:Control RAW_FILE_NAME=Ctrl_7531_13 SUBJECT_SAMPLE_FACTORS - C4_14 Factor:Control RAW_FILE_NAME=Ctrl_7531_14 SUBJECT_SAMPLE_FACTORS - C4_15 Factor:Control RAW_FILE_NAME=Ctrl_7531_15 SUBJECT_SAMPLE_FACTORS - C4_16 Factor:Control RAW_FILE_NAME=Ctrl_7531_16 SUBJECT_SAMPLE_FACTORS - C5_1 Factor:Control RAW_FILE_NAME=Ctrl_7532_1 SUBJECT_SAMPLE_FACTORS - C5_2 Factor:Control RAW_FILE_NAME=Ctrl_7532_2 SUBJECT_SAMPLE_FACTORS - C5_3 Factor:Control RAW_FILE_NAME=Ctrl_7532_3 SUBJECT_SAMPLE_FACTORS - C5_4 Factor:Control RAW_FILE_NAME=Ctrl_7532_4 SUBJECT_SAMPLE_FACTORS - C5_5 Factor:Control RAW_FILE_NAME=Ctrl_7532_5 SUBJECT_SAMPLE_FACTORS - C5_6 Factor:Control RAW_FILE_NAME=Ctrl_7532_6 SUBJECT_SAMPLE_FACTORS - C5_7 Factor:Control RAW_FILE_NAME=Ctrl_7532_7 SUBJECT_SAMPLE_FACTORS - C5_8 Factor:Control RAW_FILE_NAME=Ctrl_7532_8 SUBJECT_SAMPLE_FACTORS - C5_9 Factor:Control RAW_FILE_NAME=Ctrl_7532_9 SUBJECT_SAMPLE_FACTORS - C5_10 Factor:Control RAW_FILE_NAME=Ctrl_7532_10 SUBJECT_SAMPLE_FACTORS - C5_11 Factor:Control RAW_FILE_NAME=Ctrl_7532_11 SUBJECT_SAMPLE_FACTORS - C5_12 Factor:Control RAW_FILE_NAME=Ctrl_7532_12 SUBJECT_SAMPLE_FACTORS - C5_13 Factor:Control RAW_FILE_NAME=Ctrl_7532_13 SUBJECT_SAMPLE_FACTORS - C5_14 Factor:Control RAW_FILE_NAME=Ctrl_7532_14 SUBJECT_SAMPLE_FACTORS - C5_15 Factor:Control RAW_FILE_NAME=Ctrl_7532_15 SUBJECT_SAMPLE_FACTORS - C5_16 Factor:Control RAW_FILE_NAME=Ctrl_7532_16 SUBJECT_SAMPLE_FACTORS - C6_1 Factor:Control RAW_FILE_NAME=Ctrl_7616_1 SUBJECT_SAMPLE_FACTORS - C6_2 Factor:Control RAW_FILE_NAME=Ctrl_7616_2 SUBJECT_SAMPLE_FACTORS - C6_3 Factor:Control RAW_FILE_NAME=Ctrl_7616_3 SUBJECT_SAMPLE_FACTORS - C6_4 Factor:Control RAW_FILE_NAME=Ctrl_7616_4 SUBJECT_SAMPLE_FACTORS - C6_5 Factor:Control RAW_FILE_NAME=Ctrl_7616_5 SUBJECT_SAMPLE_FACTORS - C6_6 Factor:Control RAW_FILE_NAME=Ctrl_7616_6 SUBJECT_SAMPLE_FACTORS - C6_7 Factor:Control RAW_FILE_NAME=Ctrl_7616_7 SUBJECT_SAMPLE_FACTORS - C6_8 Factor:Control RAW_FILE_NAME=Ctrl_7616_8 SUBJECT_SAMPLE_FACTORS - C6_9 Factor:Control RAW_FILE_NAME=Ctrl_7616_9 SUBJECT_SAMPLE_FACTORS - C6_10 Factor:Control RAW_FILE_NAME=Ctrl_7616_10 SUBJECT_SAMPLE_FACTORS - C6_11 Factor:Control RAW_FILE_NAME=Ctrl_7616_11 SUBJECT_SAMPLE_FACTORS - C7_1 Factor:Control RAW_FILE_NAME=Ctrl_7781_1 SUBJECT_SAMPLE_FACTORS - C7_2 Factor:Control RAW_FILE_NAME=Ctrl_7781_2 SUBJECT_SAMPLE_FACTORS - C7_3 Factor:Control RAW_FILE_NAME=Ctrl_7781_3 SUBJECT_SAMPLE_FACTORS - C7_4 Factor:Control RAW_FILE_NAME=Ctrl_7781_4 SUBJECT_SAMPLE_FACTORS - C7_5 Factor:Control RAW_FILE_NAME=Ctrl_7781_5 SUBJECT_SAMPLE_FACTORS - C7_6 Factor:Control RAW_FILE_NAME=Ctrl_7781_6 SUBJECT_SAMPLE_FACTORS - C7_7 Factor:Control RAW_FILE_NAME=Ctrl_7781_7 SUBJECT_SAMPLE_FACTORS - C7_8 Factor:Control RAW_FILE_NAME=Ctrl_7781_8 SUBJECT_SAMPLE_FACTORS - C7_9 Factor:Control RAW_FILE_NAME=Ctrl_7781_9 SUBJECT_SAMPLE_FACTORS - C7_10 Factor:Control RAW_FILE_NAME=Ctrl_7781_10 SUBJECT_SAMPLE_FACTORS - C7_11 Factor:Control RAW_FILE_NAME=Ctrl_7781_11 SUBJECT_SAMPLE_FACTORS - C7_12 Factor:Control RAW_FILE_NAME=Ctrl_7781_12 SUBJECT_SAMPLE_FACTORS - C7_13 Factor:Control RAW_FILE_NAME=Ctrl_7781_13 SUBJECT_SAMPLE_FACTORS - C7_14 Factor:Control RAW_FILE_NAME=Ctrl_7781_14 SUBJECT_SAMPLE_FACTORS - C12_1 Factor:Control RAW_FILE_NAME=Ctrl_8469_1 SUBJECT_SAMPLE_FACTORS - C12_2 Factor:Control RAW_FILE_NAME=Ctrl_8469_2 SUBJECT_SAMPLE_FACTORS - C12_3 Factor:Control RAW_FILE_NAME=Ctrl_8469_3 SUBJECT_SAMPLE_FACTORS - C12_4 Factor:Control RAW_FILE_NAME=Ctrl_8469_4 SUBJECT_SAMPLE_FACTORS - C12_5 Factor:Control RAW_FILE_NAME=Ctrl_8469_5 SUBJECT_SAMPLE_FACTORS - C12_6 Factor:Control RAW_FILE_NAME=Ctrl_8469_6 SUBJECT_SAMPLE_FACTORS - C12_7 Factor:Control RAW_FILE_NAME=Ctrl_8469_7 SUBJECT_SAMPLE_FACTORS - C12_8 Factor:Control RAW_FILE_NAME=Ctrl_8469_8 SUBJECT_SAMPLE_FACTORS - C12_9 Factor:Control RAW_FILE_NAME=Ctrl_8469_9 SUBJECT_SAMPLE_FACTORS - C12_10 Factor:Control RAW_FILE_NAME=Ctrl_8469_10 SUBJECT_SAMPLE_FACTORS - C12_11 Factor:Control RAW_FILE_NAME=Ctrl_8469_11 SUBJECT_SAMPLE_FACTORS - C12_12 Factor:Control RAW_FILE_NAME=Ctrl_8469_12 SUBJECT_SAMPLE_FACTORS - C8_1 Factor:Control RAW_FILE_NAME=Ctrl_8103_1 SUBJECT_SAMPLE_FACTORS - C8_2 Factor:Control RAW_FILE_NAME=Ctrl_8103_2 SUBJECT_SAMPLE_FACTORS - C8_3 Factor:Control RAW_FILE_NAME=Ctrl_8103_3 SUBJECT_SAMPLE_FACTORS - C8_4 Factor:Control RAW_FILE_NAME=Ctrl_8103_4 SUBJECT_SAMPLE_FACTORS - C8_5 Factor:Control RAW_FILE_NAME=Ctrl_8103_5 SUBJECT_SAMPLE_FACTORS - C8_6 Factor:Control RAW_FILE_NAME=Ctrl_8103_6 SUBJECT_SAMPLE_FACTORS - C8_7 Factor:Control RAW_FILE_NAME=Ctrl_8103_7 SUBJECT_SAMPLE_FACTORS - C8_8 Factor:Control RAW_FILE_NAME=Ctrl_8103_8 SUBJECT_SAMPLE_FACTORS - C8_9 Factor:Control RAW_FILE_NAME=Ctrl_8103_9 SUBJECT_SAMPLE_FACTORS - C8_10 Factor:Control RAW_FILE_NAME=Ctrl_8103_10 SUBJECT_SAMPLE_FACTORS - C8_11 Factor:Control RAW_FILE_NAME=Ctrl_8103_11 SUBJECT_SAMPLE_FACTORS - C8_12 Factor:Control RAW_FILE_NAME=Ctrl_8103_12 SUBJECT_SAMPLE_FACTORS - C8_13 Factor:Control RAW_FILE_NAME=Ctrl_8103_13 SUBJECT_SAMPLE_FACTORS - C8_14 Factor:Control RAW_FILE_NAME=Ctrl_8103_14 SUBJECT_SAMPLE_FACTORS - C9_1 Factor:Control RAW_FILE_NAME=Ctrl_8257_1 SUBJECT_SAMPLE_FACTORS - C9_2 Factor:Control RAW_FILE_NAME=Ctrl_8257_2 SUBJECT_SAMPLE_FACTORS - C9_3 Factor:Control RAW_FILE_NAME=Ctrl_8257_3 SUBJECT_SAMPLE_FACTORS - C9_4 Factor:Control RAW_FILE_NAME=Ctrl_8257_4 SUBJECT_SAMPLE_FACTORS - C9_5 Factor:Control RAW_FILE_NAME=Ctrl_8257_5 SUBJECT_SAMPLE_FACTORS - C9_6 Factor:Control RAW_FILE_NAME=Ctrl_8257_6 SUBJECT_SAMPLE_FACTORS - C9_7 Factor:Control RAW_FILE_NAME=Ctrl_8257_7 SUBJECT_SAMPLE_FACTORS - C9_8 Factor:Control RAW_FILE_NAME=Ctrl_8257_8 SUBJECT_SAMPLE_FACTORS - C9_9 Factor:Control RAW_FILE_NAME=Ctrl_8257_9 SUBJECT_SAMPLE_FACTORS - C9_10 Factor:Control RAW_FILE_NAME=Ctrl_8257_10 SUBJECT_SAMPLE_FACTORS - C9_11 Factor:Control RAW_FILE_NAME=Ctrl_8257_11 SUBJECT_SAMPLE_FACTORS - C9_12 Factor:Control RAW_FILE_NAME=Ctrl_8257_12 SUBJECT_SAMPLE_FACTORS - C9_13 Factor:Control RAW_FILE_NAME=Ctrl_8257_13 SUBJECT_SAMPLE_FACTORS - C9_14 Factor:Control RAW_FILE_NAME=Ctrl_8257_14 SUBJECT_SAMPLE_FACTORS - C9_15 Factor:Control RAW_FILE_NAME=Ctrl_8257_15 SUBJECT_SAMPLE_FACTORS - C9_16 Factor:Control RAW_FILE_NAME=Ctrl_8257_16 SUBJECT_SAMPLE_FACTORS - C10_1 Factor:Control RAW_FILE_NAME=Ctrl_8284_1 SUBJECT_SAMPLE_FACTORS - C10_2 Factor:Control RAW_FILE_NAME=Ctrl_8284_2 SUBJECT_SAMPLE_FACTORS - C10_3 Factor:Control RAW_FILE_NAME=Ctrl_8284_3 SUBJECT_SAMPLE_FACTORS - C10_4 Factor:Control RAW_FILE_NAME=Ctrl_8284_4 SUBJECT_SAMPLE_FACTORS - C10_5 Factor:Control RAW_FILE_NAME=Ctrl_8284_5 SUBJECT_SAMPLE_FACTORS - C10_6 Factor:Control RAW_FILE_NAME=Ctrl_8284_6 SUBJECT_SAMPLE_FACTORS - C10_7 Factor:Control RAW_FILE_NAME=Ctrl_8284_7 SUBJECT_SAMPLE_FACTORS - C10_8 Factor:Control RAW_FILE_NAME=Ctrl_8284_8 SUBJECT_SAMPLE_FACTORS - C10_9 Factor:Control RAW_FILE_NAME=Ctrl_8284_9 SUBJECT_SAMPLE_FACTORS - C10_10 Factor:Control RAW_FILE_NAME=Ctrl_8284_10 SUBJECT_SAMPLE_FACTORS - C10_11 Factor:Control RAW_FILE_NAME=Ctrl_8284_11 SUBJECT_SAMPLE_FACTORS - C10_12 Factor:Control RAW_FILE_NAME=Ctrl_8284_12 SUBJECT_SAMPLE_FACTORS - C11_1 Factor:Control RAW_FILE_NAME=Ctrl_8411_1 SUBJECT_SAMPLE_FACTORS - C11_2 Factor:Control RAW_FILE_NAME=Ctrl_8411_2 SUBJECT_SAMPLE_FACTORS - C11_3 Factor:Control RAW_FILE_NAME=Ctrl_8411_3 SUBJECT_SAMPLE_FACTORS - C11_4 Factor:Control RAW_FILE_NAME=Ctrl_8411_4 SUBJECT_SAMPLE_FACTORS - C11_5 Factor:Control RAW_FILE_NAME=Ctrl_8411_5 SUBJECT_SAMPLE_FACTORS - C11_6 Factor:Control RAW_FILE_NAME=Ctrl_8411_6 SUBJECT_SAMPLE_FACTORS - C11_7 Factor:Control RAW_FILE_NAME=Ctrl_8411_7 SUBJECT_SAMPLE_FACTORS - C11_8 Factor:Control RAW_FILE_NAME=Ctrl_8411_8 SUBJECT_SAMPLE_FACTORS - C11_9 Factor:Control RAW_FILE_NAME=Ctrl_8411_9 SUBJECT_SAMPLE_FACTORS - C11_10 Factor:Control RAW_FILE_NAME=Ctrl_8411_10 SUBJECT_SAMPLE_FACTORS - C11_11 Factor:Control RAW_FILE_NAME=Ctrl_8411_11 SUBJECT_SAMPLE_FACTORS - C11_12 Factor:Control RAW_FILE_NAME=Ctrl_8411_12 SUBJECT_SAMPLE_FACTORS - C11_13 Factor:Control RAW_FILE_NAME=Ctrl_8411_13 SUBJECT_SAMPLE_FACTORS - C13_1 Factor:Control RAW_FILE_NAME=Ctrl_8512_1 SUBJECT_SAMPLE_FACTORS - C13_2 Factor:Control RAW_FILE_NAME=Ctrl_8512_2 SUBJECT_SAMPLE_FACTORS - C13_3 Factor:Control RAW_FILE_NAME=Ctrl_8512_3 SUBJECT_SAMPLE_FACTORS - C13_4 Factor:Control RAW_FILE_NAME=Ctrl_8512_4 SUBJECT_SAMPLE_FACTORS - C13_5 Factor:Control RAW_FILE_NAME=Ctrl_8512_5 SUBJECT_SAMPLE_FACTORS - C13_6 Factor:Control RAW_FILE_NAME=Ctrl_8512_6 SUBJECT_SAMPLE_FACTORS - C13_7 Factor:Control RAW_FILE_NAME=Ctrl_8512_7 SUBJECT_SAMPLE_FACTORS - C13_8 Factor:Control RAW_FILE_NAME=Ctrl_8512_8 SUBJECT_SAMPLE_FACTORS - C13_9 Factor:Control RAW_FILE_NAME=Ctrl_8512_9 SUBJECT_SAMPLE_FACTORS - C13_10 Factor:Control RAW_FILE_NAME=Ctrl_8512_10 SUBJECT_SAMPLE_FACTORS - C13_11 Factor:Control RAW_FILE_NAME=Ctrl_8512_11 SUBJECT_SAMPLE_FACTORS - C13_12 Factor:Control RAW_FILE_NAME=Ctrl_8512_12 SUBJECT_SAMPLE_FACTORS - C13_13 Factor:Control RAW_FILE_NAME=Ctrl_8512_13 SUBJECT_SAMPLE_FACTORS - C14_1 Factor:Control RAW_FILE_NAME=Ctrl_8517_1 SUBJECT_SAMPLE_FACTORS - C14_2 Factor:Control RAW_FILE_NAME=Ctrl_8517_2 SUBJECT_SAMPLE_FACTORS - C14_3 Factor:Control RAW_FILE_NAME=Ctrl_8517_3 SUBJECT_SAMPLE_FACTORS - C14_4 Factor:Control RAW_FILE_NAME=Ctrl_8517_4 SUBJECT_SAMPLE_FACTORS - C14_5 Factor:Control RAW_FILE_NAME=Ctrl_8517_5 SUBJECT_SAMPLE_FACTORS - C14_6 Factor:Control RAW_FILE_NAME=Ctrl_8517_6 SUBJECT_SAMPLE_FACTORS - C14_7 Factor:Control RAW_FILE_NAME=Ctrl_8517_7 SUBJECT_SAMPLE_FACTORS - C14_8 Factor:Control RAW_FILE_NAME=Ctrl_8517_8 SUBJECT_SAMPLE_FACTORS - C14_9 Factor:Control RAW_FILE_NAME=Ctrl_8517_9 SUBJECT_SAMPLE_FACTORS - C14_10 Factor:Control RAW_FILE_NAME=Ctrl_8517_10 SUBJECT_SAMPLE_FACTORS - C14_11 Factor:Control RAW_FILE_NAME=Ctrl_8517_11 SUBJECT_SAMPLE_FACTORS - C14_12 Factor:Control RAW_FILE_NAME=Ctrl_8517_12 SUBJECT_SAMPLE_FACTORS - C14_13 Factor:Control RAW_FILE_NAME=Ctrl_8517_13 SUBJECT_SAMPLE_FACTORS - C15_1 Factor:Control RAW_FILE_NAME=Ctrl_8748_1 SUBJECT_SAMPLE_FACTORS - C15_2 Factor:Control RAW_FILE_NAME=Ctrl_8748_2 SUBJECT_SAMPLE_FACTORS - C15_3 Factor:Control RAW_FILE_NAME=Ctrl_8748_3 SUBJECT_SAMPLE_FACTORS - C15_4 Factor:Control RAW_FILE_NAME=Ctrl_8748_4 SUBJECT_SAMPLE_FACTORS - C15_5 Factor:Control RAW_FILE_NAME=Ctrl_8748_5 SUBJECT_SAMPLE_FACTORS - C15_6 Factor:Control RAW_FILE_NAME=Ctrl_8748_6 SUBJECT_SAMPLE_FACTORS - C15_7 Factor:Control RAW_FILE_NAME=Ctrl_8748_7 SUBJECT_SAMPLE_FACTORS - C15_8 Factor:Control RAW_FILE_NAME=Ctrl_8748_8 SUBJECT_SAMPLE_FACTORS - C15_9 Factor:Control RAW_FILE_NAME=Ctrl_8748_9 SUBJECT_SAMPLE_FACTORS - C15_10 Factor:Control RAW_FILE_NAME=Ctrl_8748_10 SUBJECT_SAMPLE_FACTORS - C15_11 Factor:Control RAW_FILE_NAME=Ctrl_8748_11 SUBJECT_SAMPLE_FACTORS - C15_12 Factor:Control RAW_FILE_NAME=Ctrl_8748_12 SUBJECT_SAMPLE_FACTORS - C16_1 Factor:Control RAW_FILE_NAME=Ctrl_8749_1 SUBJECT_SAMPLE_FACTORS - C16_2 Factor:Control RAW_FILE_NAME=Ctrl_8749_2 SUBJECT_SAMPLE_FACTORS - C16_3 Factor:Control RAW_FILE_NAME=Ctrl_8749_3 SUBJECT_SAMPLE_FACTORS - C16_4 Factor:Control RAW_FILE_NAME=Ctrl_8749_4 SUBJECT_SAMPLE_FACTORS - C16_5 Factor:Control RAW_FILE_NAME=Ctrl_8749_5 SUBJECT_SAMPLE_FACTORS - C16_6 Factor:Control RAW_FILE_NAME=Ctrl_8749_6 SUBJECT_SAMPLE_FACTORS - C16_7 Factor:Control RAW_FILE_NAME=Ctrl_8749_7 SUBJECT_SAMPLE_FACTORS - C16_8 Factor:Control RAW_FILE_NAME=Ctrl_8749_8 SUBJECT_SAMPLE_FACTORS - C16_9 Factor:Control RAW_FILE_NAME=Ctrl_8749_9 SUBJECT_SAMPLE_FACTORS - C16_10 Factor:Control RAW_FILE_NAME=Ctrl_8749_10 SUBJECT_SAMPLE_FACTORS - C16_11 Factor:Control RAW_FILE_NAME=Ctrl_8749_11 SUBJECT_SAMPLE_FACTORS - C16_12 Factor:Control RAW_FILE_NAME=Ctrl_8749_12 SUBJECT_SAMPLE_FACTORS - - Factor:- RAW_FILE_NAME=- #COLLECTION CO:COLLECTION_SUMMARY Blood samples were collected from 17 TKO mice and 16 TKO control mice starting CO:COLLECTION_SUMMARY at 8 weeks of age. A sequential blood sampling procedure was conducted and CO:COLLECTION_SUMMARY samples from each mouse were collected every two weeks until end point or CO:COLLECTION_SUMMARY ascites. Two TKO mice died after 14 and 16 weeks, and were not included in the CO:COLLECTION_SUMMARY UHPLC-MS analysis. One TKO control mouse died after 10 weeks and was also CO:COLLECTION_SUMMARY excluded. CO:SAMPLE_TYPE Blood (serum) #TREATMENT TR:TREATMENT_SUMMARY p53LSL R172H/+ Dicer1flox/flox Ptenflox/flox Amhr2cre/+ mice were generated by TR:TREATMENT_SUMMARY mating p53LSL-R172H/+Dicer1flox/floxPtenflox/flox female mice with TR:TREATMENT_SUMMARY Dicer1flox/floxPtenflox/floxAmhr2cre/+ male mice. TR:TREATMENT_SUMMARY p53LSL-R172H/+Dicer1flox/floxPtenflox/flox mice were used as TKO controls TR:TREATMENT_SUMMARY (ctrl). TKO ctrl mice carry the same genetic background as TKO mice but do not TR:TREATMENT_SUMMARY develop HGSC. p53LSL R172H/+ Dicer1flox/flox Ptenflox/flox Amhr2cre/+ mice were TR:TREATMENT_SUMMARY sacrificed in accordance to the animal protocol approved by the institutional TR:TREATMENT_SUMMARY animal care and use committee (IACUC) at Indiana University. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Serum samples were thawed on ice, followed by metabolite extraction of both SP:SAMPLEPREP_SUMMARY non-polar (lipid) and polar metabolites using two different sample preparation SP:SAMPLEPREP_SUMMARY protocols. The extraction solvent for RP UHPLC-MS was prepared by adding the SP:SAMPLEPREP_SUMMARY isotopically labeled lipid standard mixture to 2-propanol in a 1:60 ratio. SP:SAMPLEPREP_SUMMARY Likewise, for HILIC UHPLC-MS, the extraction solvent was prepared by mixing an SP:SAMPLEPREP_SUMMARY isotopically labeled mixture of polar metabolites and methanol in a 1:60 ratio. SP:SAMPLEPREP_SUMMARY These extraction solvents were added to serum samples in a 3:1 ratio to SP:SAMPLEPREP_SUMMARY precipitate proteins. Following this step, samples were vortex-mixed for 30 SP:SAMPLEPREP_SUMMARY seconds and centrifuged at 13,000 rpm for 7 minutes. The resulting supernatant SP:SAMPLEPREP_SUMMARY was transferred to snap-on LC vials and stored at -80 °C until UHPLC-MS SP:SAMPLEPREP_SUMMARY analysis, which was performed within a week. A blank sample, prepared with LC-MS SP:SAMPLEPREP_SUMMARY grade water, and 2-propanol (or methanol) underwent the same preparation process SP:SAMPLEPREP_SUMMARY as the serum samples, and analyzed together simultaneously. A pooled quality SP:SAMPLEPREP_SUMMARY control (QC) was prepared by mixing 5-10 μL aliquots of the extract of each SP:SAMPLEPREP_SUMMARY serum sample. This pooled sample was analyzed every 10 runs to monitor LC-MS SP:SAMPLEPREP_SUMMARY instrument stability and latter correct for any sensitivity drift. Samples were SP:SAMPLEPREP_SUMMARY run in a randomized order on consecutive days. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 CH:COLUMN_NAME Thermo Accucore C30 (150, 2.1mm,2.6um) CH:SOLVENT_A 10 mM ammonium acetate with water/acetonitrile (40:60 v/v) CH:SOLVENT_B 10 mM ammonium acetate with 2-isopropanol/acetonitrile (90:10 v/v). 0.1% formic CH:SOLVENT_B acid #ANALYSIS AN:ANALYSIS_TYPE MS AN:DATA_FORMAT .raw #MS MS:INSTRUMENT_NAME Thermo Q Exactive HF hybrid Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS Samples were analyzed in positive and negative ion modes for RP chromatography. MS:MS_COMMENTS Spectral features were extracted using Compound Discoverer v3.0. MS:CAPILLARY_TEMPERATURE 275 °C MS:MS_RESULTS_FILE ST002067_AN003367_Results.txt UNITS:chromatographic peak area Has m/z:Neutral masses Has RT:Yes RT units:Minutes #END