#METABOLOMICS WORKBENCH ZJ309_20220409_201019_mwtab.txt DATATRACK_ID:3186 STUDY_ID:ST002154 ANALYSIS_ID:AN003528 PROJECT_ID:PR001367 VERSION 1 CREATED_ON April 9, 2022, 8:18 pm #PROJECT PR:PROJECT_TITLE Metabolomics Insights into the Interaction between Pseudomonas plecoglossicida PR:PROJECT_TITLE and Epinephelus coioides PR:PROJECT_SUMMARY Here, a P. plecoglossicida strain (NZBD9) and economic fish of Epinephelus PR:PROJECT_SUMMARY coioides were investigated as a model system to characterize pathogen-induced PR:PROJECT_SUMMARY host metabolic remodeling over the course of infection. We present a nontargeted PR:PROJECT_SUMMARY metabolomics profiling of E. coioides spleens from uninfected E. coioides and PR:PROJECT_SUMMARY those infected with wild-type and clpV-RNA interference (RNAi) strains. PR:INSTITUTE College of Marine Food and Biological Engineering, Jimei University PR:LABORATORY Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China PR:LAST_NAME Yang PR:FIRST_NAME Zhiqiang PR:ADDRESS No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, China PR:EMAIL 201911832016@jmu.edu.cn PR:PHONE 86-592-6181487 #STUDY ST:STUDY_TITLE Virus-host interaction studies of P. plecoglossicida strain (NZBD9) and ST:STUDY_TITLE Epinephelus coioides. ST:STUDY_SUMMARY E. coioides of the infection group were intrapleurally injected with 105 ST:STUDY_SUMMARY colony-forming units per fish (cfu/fish) of P. plecoglossicida (wild-type strain ST:STUDY_SUMMARY or clpV-RNAi strain), while control E. coioides received an equivalent volume of ST:STUDY_SUMMARY phosphate-buffered saline (PBS). The temperature of the water was maintained at ST:STUDY_SUMMARY 18 ± 1 °C during the infection experiment. The spleens of E. coioides were ST:STUDY_SUMMARY collected at 6, 12, 24, 48, 72 and 96 hours post injection (hpi). ST:INSTITUTE Jimei University ST:LAST_NAME Yang ST:FIRST_NAME Zhiqiang ST:ADDRESS No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, No. 185 ST:ADDRESS Yinjiang Road ST:EMAIL 201911832016@jmu.edu.cn ST:PHONE 5926181487 #SUBJECT SU:SUBJECT_TYPE Fish SU:SUBJECT_SPECIES Epinephelus coioides #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - PBS6-1-m Treatment:Control Model=Positive; RAW_FILE_NAME=PBS6-1-m.RAW SUBJECT_SAMPLE_FACTORS - PBS6-2-m Treatment:Control Model=Positive; RAW_FILE_NAME=PBS6-2-m.RAW SUBJECT_SAMPLE_FACTORS - PBS6-3-m Treatment:Control Model=Positive; RAW_FILE_NAME=PBS6-3-m.RAW SUBJECT_SAMPLE_FACTORS - PBS6-4-m Treatment:Control Model=Positive; RAW_FILE_NAME=PBS6-4-m.RAW SUBJECT_SAMPLE_FACTORS - PBS6-5-m Treatment:Control Model=Positive; RAW_FILE_NAME=PBS6-5-m.RAW SUBJECT_SAMPLE_FACTORS - PBS6-6-m Treatment:Control Model=Positive; 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RAW_FILE_NAME=wt1d-1-m.RAW SUBJECT_SAMPLE_FACTORS - wt1d-2-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt1d-2-m.RAW SUBJECT_SAMPLE_FACTORS - wt1d-3-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt1d-3-m.RAW SUBJECT_SAMPLE_FACTORS - wt1d-4-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt1d-4-m.RAW SUBJECT_SAMPLE_FACTORS - wt1d-5-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt1d-5-m.RAW SUBJECT_SAMPLE_FACTORS - wt1d-6-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt1d-6-m.RAW SUBJECT_SAMPLE_FACTORS - wt1d-7-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt1d-7-m.RAW SUBJECT_SAMPLE_FACTORS - wt2d-1-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt2d-1-m.RAW SUBJECT_SAMPLE_FACTORS - wt2d-2-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt2d-2-m.RAW SUBJECT_SAMPLE_FACTORS - wt2d-3-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt2d-3-m.RAW SUBJECT_SAMPLE_FACTORS - wt2d-4-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt2d-4-m.RAW SUBJECT_SAMPLE_FACTORS - wt2d-5-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt2d-5-m.RAW SUBJECT_SAMPLE_FACTORS - wt2d-6-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt2d-6-m.RAW SUBJECT_SAMPLE_FACTORS - wt2d-7-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt2d-7-m.RAW SUBJECT_SAMPLE_FACTORS - wt3d-1-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt3d-1-m.RAW SUBJECT_SAMPLE_FACTORS - wt3d-2-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt3d-2-m.RAW SUBJECT_SAMPLE_FACTORS - wt3d-3-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt3d-3-m.RAW SUBJECT_SAMPLE_FACTORS - wt3d-4-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt3d-4-m.RAW SUBJECT_SAMPLE_FACTORS - wt3d-5-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt3d-5-m.RAW SUBJECT_SAMPLE_FACTORS - wt3d-6-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt3d-6-m.RAW SUBJECT_SAMPLE_FACTORS - wt3d-7-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt3d-7-m.RAW SUBJECT_SAMPLE_FACTORS - wt4d-1-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt4d-1-m.RAW SUBJECT_SAMPLE_FACTORS - wt4d-2-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt4d-2-m.RAW SUBJECT_SAMPLE_FACTORS - wt4d-3-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt4d-3-m.RAW SUBJECT_SAMPLE_FACTORS - wt4d-4-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt4d-4-m.RAW SUBJECT_SAMPLE_FACTORS - wt4d-5-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt4d-5-m.RAW SUBJECT_SAMPLE_FACTORS - wt4d-6-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt4d-6-m.RAW SUBJECT_SAMPLE_FACTORS - wt4d-7-m Treatment:wild-type strain infection group Model=Positive; RAW_FILE_NAME=wt4d-7-m.RAW SUBJECT_SAMPLE_FACTORS - clpv6-1-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv6-1-m.RAW SUBJECT_SAMPLE_FACTORS - clpv6-2-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv6-2-m.RAW SUBJECT_SAMPLE_FACTORS - clpv6-3-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv6-3-m.RAW SUBJECT_SAMPLE_FACTORS - clpv6-4-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv6-4-m.RAW SUBJECT_SAMPLE_FACTORS - clpv6-5-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv6-5-m.RAW SUBJECT_SAMPLE_FACTORS - clpv6-6-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv6-6-m.RAW SUBJECT_SAMPLE_FACTORS - clpv6-7-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv6-7-m.RAW SUBJECT_SAMPLE_FACTORS - clpv12-1-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv12-1-m.RAW SUBJECT_SAMPLE_FACTORS - clpv12-2-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv12-2-m.RAW SUBJECT_SAMPLE_FACTORS - clpv12-3-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv12-3-m.RAW SUBJECT_SAMPLE_FACTORS - clpv12-4-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv12-4-m.RAW SUBJECT_SAMPLE_FACTORS - clpv12-5-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv12-5-m.RAW SUBJECT_SAMPLE_FACTORS - clpv12-6-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv12-6-m.RAW SUBJECT_SAMPLE_FACTORS - clpv12-7-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv12-7-m.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-1-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv1d-1-m.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-2-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv1d-2-m.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-3-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv1d-3-m.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-4-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv1d-4-m.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-5-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv1d-5-m.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-6-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv1d-6-m.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-7-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv1d-7-m.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-1-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv2d-1-m.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-2-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv2d-2-m.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-3-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv2d-3-m.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-4-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv2d-4-m.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-5-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv2d-5-m.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-6-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv2d-6-m.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-7-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv2d-7-m.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-1-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv3d-1-m.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-2-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv3d-2-m.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-3-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv3d-3-m.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-4-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv3d-4-m.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-5-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv3d-5-m.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-6-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv3d-6-m.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-7-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv3d-7-m.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-1-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv4d-1-m.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-2-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv4d-2-m.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-3-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv4d-3-m.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-4-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv4d-4-m.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-5-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv4d-5-m.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-6-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv4d-6-m.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-7-m Treatment:clpV-RNAi strain infection group Model=Positive; RAW_FILE_NAME=clpv4d-7-m.RAW SUBJECT_SAMPLE_FACTORS - PBS6-1 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS6-1.RAW SUBJECT_SAMPLE_FACTORS - PBS6-2 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS6-2.RAW SUBJECT_SAMPLE_FACTORS - PBS6-3 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS6-3.RAW SUBJECT_SAMPLE_FACTORS - PBS6-4 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS6-4.RAW SUBJECT_SAMPLE_FACTORS - PBS6-5 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS6-5.RAW SUBJECT_SAMPLE_FACTORS - PBS6-6 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS6-6.RAW SUBJECT_SAMPLE_FACTORS - PBS6-7 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS6-7.RAW SUBJECT_SAMPLE_FACTORS - PBS12-1 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS12-1.RAW SUBJECT_SAMPLE_FACTORS - PBS12-2 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS12-2.RAW SUBJECT_SAMPLE_FACTORS - PBS12-3 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS12-3.RAW SUBJECT_SAMPLE_FACTORS - PBS12-4 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS12-4.RAW SUBJECT_SAMPLE_FACTORS - PBS12-5 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS12-5.RAW SUBJECT_SAMPLE_FACTORS - PBS12-6 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS12-6.RAW SUBJECT_SAMPLE_FACTORS - PBS12-7 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS12-7.RAW SUBJECT_SAMPLE_FACTORS - PBS1d-1 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS1d-1.RAW SUBJECT_SAMPLE_FACTORS - PBS1d-2 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS1d-2.RAW SUBJECT_SAMPLE_FACTORS - PBS1d-3 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS1d-3.RAW SUBJECT_SAMPLE_FACTORS - PBS1d-4 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS1d-4.RAW SUBJECT_SAMPLE_FACTORS - PBS1d-5 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS1d-5.RAW SUBJECT_SAMPLE_FACTORS - PBS1d-6 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS1d-6.RAW SUBJECT_SAMPLE_FACTORS - PBS1d-7 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS1d-7.RAW SUBJECT_SAMPLE_FACTORS - PBS2d-1 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS2d-1.RAW SUBJECT_SAMPLE_FACTORS - PBS2d-2 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS2d-2.RAW SUBJECT_SAMPLE_FACTORS - PBS2d-3 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS2d-3.RAW SUBJECT_SAMPLE_FACTORS - PBS2d-4 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS2d-4.RAW SUBJECT_SAMPLE_FACTORS - PBS2d-5 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS2d-5.RAW SUBJECT_SAMPLE_FACTORS - PBS2d-6 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS2d-6.RAW SUBJECT_SAMPLE_FACTORS - PBS2d-7 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS2d-7.RAW SUBJECT_SAMPLE_FACTORS - PBS3d-1 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS3d-1.RAW SUBJECT_SAMPLE_FACTORS - PBS3d-2 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS3d-2.RAW SUBJECT_SAMPLE_FACTORS - PBS3d-3 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS3d-3.RAW SUBJECT_SAMPLE_FACTORS - PBS3d-4 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS3d-4.RAW SUBJECT_SAMPLE_FACTORS - PBS3d-5 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS3d-5.RAW SUBJECT_SAMPLE_FACTORS - PBS3d-6 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS3d-6.RAW SUBJECT_SAMPLE_FACTORS - PBS3d-7 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS3d-7.RAW SUBJECT_SAMPLE_FACTORS - PBS4d-1 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS4d-1.RAW SUBJECT_SAMPLE_FACTORS - PBS4d-2 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS4d-2.RAW SUBJECT_SAMPLE_FACTORS - PBS4d-3 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS4d-3.RAW SUBJECT_SAMPLE_FACTORS - PBS4d-4 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS4d-4.RAW SUBJECT_SAMPLE_FACTORS - PBS4d-5 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS4d-5.RAW SUBJECT_SAMPLE_FACTORS - PBS4d-6 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS4d-6.RAW SUBJECT_SAMPLE_FACTORS - PBS4d-7 Treatment:Control Model=Negative; RAW_FILE_NAME=PBS4d-7.RAW SUBJECT_SAMPLE_FACTORS - wt6-1 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt6-1.RAW SUBJECT_SAMPLE_FACTORS - wt6-2 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt6-2.RAW SUBJECT_SAMPLE_FACTORS - wt6-3 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt6-3.RAW SUBJECT_SAMPLE_FACTORS - wt6-4 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt6-4.RAW SUBJECT_SAMPLE_FACTORS - wt6-5 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt6-5.RAW SUBJECT_SAMPLE_FACTORS - wt6-6 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt6-6.RAW SUBJECT_SAMPLE_FACTORS - wt6-7 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt6-7.RAW SUBJECT_SAMPLE_FACTORS - wt12-1 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt12-1.RAW SUBJECT_SAMPLE_FACTORS - wt12-2 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt12-2.RAW SUBJECT_SAMPLE_FACTORS - wt12-3 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt12-3.RAW SUBJECT_SAMPLE_FACTORS - wt12-4 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt12-4.RAW SUBJECT_SAMPLE_FACTORS - wt12-5 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt12-5.RAW SUBJECT_SAMPLE_FACTORS - wt12-6 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt12-6.RAW SUBJECT_SAMPLE_FACTORS - wt12-7 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt12-7.RAW SUBJECT_SAMPLE_FACTORS - wt1d-1 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt1d-1.RAW SUBJECT_SAMPLE_FACTORS - wt1d-2 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt1d-2.RAW SUBJECT_SAMPLE_FACTORS - wt1d-3 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt1d-3.RAW SUBJECT_SAMPLE_FACTORS - wt1d-4 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt1d-4.RAW SUBJECT_SAMPLE_FACTORS - wt1d-5 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt1d-5.RAW SUBJECT_SAMPLE_FACTORS - wt1d-6 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt1d-6.RAW SUBJECT_SAMPLE_FACTORS - wt1d-7 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt1d-7.RAW SUBJECT_SAMPLE_FACTORS - wt2d-1 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt2d-1.RAW SUBJECT_SAMPLE_FACTORS - wt2d-2 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt2d-2.RAW SUBJECT_SAMPLE_FACTORS - wt2d-3 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt2d-3.RAW SUBJECT_SAMPLE_FACTORS - wt2d-4 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt2d-4.RAW SUBJECT_SAMPLE_FACTORS - wt2d-5 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt2d-5.RAW SUBJECT_SAMPLE_FACTORS - wt2d-6 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt2d-6.RAW SUBJECT_SAMPLE_FACTORS - wt2d-7 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt2d-7.RAW SUBJECT_SAMPLE_FACTORS - wt3d-1 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt3d-1.RAW SUBJECT_SAMPLE_FACTORS - wt3d-2 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt3d-2.RAW SUBJECT_SAMPLE_FACTORS - wt3d-3 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt3d-3.RAW SUBJECT_SAMPLE_FACTORS - wt3d-4 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt3d-4.RAW SUBJECT_SAMPLE_FACTORS - wt3d-5 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt3d-5.RAW SUBJECT_SAMPLE_FACTORS - wt3d-6 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt3d-6.RAW SUBJECT_SAMPLE_FACTORS - wt3d-7 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt3d-7.RAW SUBJECT_SAMPLE_FACTORS - wt4d-1 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt4d-1.RAW SUBJECT_SAMPLE_FACTORS - wt4d-2 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt4d-2.RAW SUBJECT_SAMPLE_FACTORS - wt4d-3 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt4d-3.RAW SUBJECT_SAMPLE_FACTORS - wt4d-4 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt4d-4.RAW SUBJECT_SAMPLE_FACTORS - wt4d-5 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt4d-5.RAW SUBJECT_SAMPLE_FACTORS - wt4d-6 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt4d-6.RAW SUBJECT_SAMPLE_FACTORS - wt4d-7 Treatment:wild-type strain infection group Model=Negative; RAW_FILE_NAME=wt4d-7.RAW SUBJECT_SAMPLE_FACTORS - clpv6-1 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv6-1.RAW SUBJECT_SAMPLE_FACTORS - clpv6-2 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv6-2.RAW SUBJECT_SAMPLE_FACTORS - clpv6-3 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv6-3.RAW SUBJECT_SAMPLE_FACTORS - clpv6-4 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv6-4.RAW SUBJECT_SAMPLE_FACTORS - clpv6-5 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv6-5.RAW SUBJECT_SAMPLE_FACTORS - clpv6-6 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv6-6.RAW SUBJECT_SAMPLE_FACTORS - clpv6-7 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv6-7.RAW SUBJECT_SAMPLE_FACTORS - clpv12-1 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv12-1.RAW SUBJECT_SAMPLE_FACTORS - clpv12-2 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv12-2.RAW SUBJECT_SAMPLE_FACTORS - clpv12-3 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv12-3.RAW SUBJECT_SAMPLE_FACTORS - clpv12-4 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv12-4.RAW SUBJECT_SAMPLE_FACTORS - clpv12-5 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv12-5.RAW SUBJECT_SAMPLE_FACTORS - clpv12-6 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv12-6.RAW SUBJECT_SAMPLE_FACTORS - clpv12-7 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv12-7.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-1 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv1d-1.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-2 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv1d-2.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-3 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv1d-3.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-4 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv1d-4.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-5 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv1d-5.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-6 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv1d-6.RAW SUBJECT_SAMPLE_FACTORS - clpv1d-7 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv1d-7.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-1 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv2d-1.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-2 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv2d-2.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-3 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv2d-3.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-4 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv2d-4.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-5 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv2d-5.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-6 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv2d-6.RAW SUBJECT_SAMPLE_FACTORS - clpv2d-7 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv2d-7.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-1 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv3d-1.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-2 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv3d-2.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-3 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv3d-3.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-4 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv3d-4.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-5 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv3d-5.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-6 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv3d-6.RAW SUBJECT_SAMPLE_FACTORS - clpv3d-7 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv3d-7.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-1 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv4d-1.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-2 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv4d-2.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-3 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv4d-3.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-4 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv4d-4.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-5 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv4d-5.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-6 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv4d-6.RAW SUBJECT_SAMPLE_FACTORS - clpv4d-7 Treatment:clpV-RNAi strain infection group Model=Negative; RAW_FILE_NAME=clpv4d-7.RAW #COLLECTION CO:COLLECTION_SUMMARY The spleens of E. coioides were collected at 6, 12, 24, 48, 72 and 96 hours post CO:COLLECTION_SUMMARY injection (hpi). After phenotypic observation of spleen tissue, grouper spleen CO:COLLECTION_SUMMARY samples were collected and accurately weighed (~15 mg) and stored in a -80 °C CO:COLLECTION_SUMMARY freezer. CO:COLLECTION_PROTOCOL_COMMENTS E.coioides.pdf CO:SAMPLE_TYPE Spleen CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY E. coioides of the infection group were intrapleurally injected with 105 TR:TREATMENT_SUMMARY colony-forming units per fish (cfu/fish) of P. plecoglossicida (wild-type strain TR:TREATMENT_SUMMARY or clpV-RNAi strain), while control E. coioides received an equivalent volume of TR:TREATMENT_SUMMARY phosphate-buffered saline (PBS). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY The deep-frozen spleen tissue in each replicate was accurately weighed (about 15 SP:SAMPLEPREP_SUMMARY mg) and then transferred to an Eppendorf tube. Each sample was first spiked with SP:SAMPLEPREP_SUMMARY 300 μL of methanol containing internal standards and then homogenized at 65 Hz SP:SAMPLEPREP_SUMMARY for 2.5 min. The homogenate was mixed with 1 mL of MTBE, and thoroughly vortexed SP:SAMPLEPREP_SUMMARY for 30 min. Then, 300 μL of Milli-Q water was added to the tube, followed by SP:SAMPLEPREP_SUMMARY vortexing for 1 min and centrifugation for 10 min to form a two-phase system SP:SAMPLEPREP_SUMMARY (12,000 rpm, 4 ℃). Each specimen was prepared by mixing 250 μL of the top SP:SAMPLEPREP_SUMMARY layer plus 150 μL of the bottom layer accurately and then vacuum-dried in a SP:SAMPLEPREP_SUMMARY Speedvac concentrator (Thermo Scientific, USA). #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Waters Acquity BEH C8 (100 x 2.1mm, 1.7um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive HF-X Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS - MS:MS_RESULTS_FILE ST002154_AN003528_Results.txt UNITS:Peak area Has m/z:Yes Has RT:No RT units:Minutes #END