{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002156","ANALYSIS_ID":"AN003531","VERSION":"1","CREATED_ON":"May 3, 2022, 11:39 am"}, "PROJECT":{"PROJECT_TITLE":"Contribution of western diet to NASH pathogenesis by defining liver metabolites","PROJECT_SUMMARY":"Western diet (WD) and gut microbiota interplay drives the development of non-alcoholic fatty liver disease (NAFLD) and its progression to non-alcoholic steatohepatitis (NASH). However, the metabolic mediators contributing to NASH remain to be identified. In this study, we explored the change of liver metabolites in a diet-induced mouse NASH model. Identification of these metabolites will help to uncover the pathogenesis of NASH and explore the new therapeutic treatment.","INSTITUTE":"University of Missouri-Columbia","LAST_NAME":"Yang","FIRST_NAME":"Ming","ADDRESS":"University of Missouri, NextGen Prevision Health Building, Room 2203","EMAIL":"yangmin@health.missouri.edu","PHONE":"573-882-7141"}, "STUDY":{"STUDY_TITLE":"Mouse liver metabolites","STUDY_SUMMARY":"Western diet (WD) and gut microbiota interplay drives the development of non-alcoholic fatty liver disease (NAFLD) and its progression to non-alcoholic steatohepatitis (NASH). However, the metabolic mediators contributing to NASH remain to be identified. In this study, we explored the change of liver metabolites in a diet-induced mouse NASH model. Identification of these metabolites will help to uncover the pathogenesis of NASH and explore the new therapeutic treatment.","INSTITUTE":"University of Missouri-Columbia","LAST_NAME":"Yang","FIRST_NAME":"Ming","ADDRESS":"University of Missouri","EMAIL":"yangmin@health.missouri.edu","PHONE":"5738827141"}, "SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Male"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"C1", "Factors":{"raw_data_time_format":"High-fat diet"} }, { "Subject ID":"-", "Sample ID":"C2", "Factors":{"raw_data_time_format":"High-fat diet"} }, { "Subject ID":"-", "Sample ID":"C3", "Factors":{"raw_data_time_format":"High-fat diet"} }, { "Subject ID":"-", "Sample ID":"C4", "Factors":{"raw_data_time_format":"High-fat diet"} }, { "Subject ID":"-", "Sample ID":"C5", "Factors":{"raw_data_time_format":"High-fat diet"} }, { "Subject ID":"-", "Sample ID":"C6", "Factors":{"raw_data_time_format":"High-fat diet"} }, { "Subject ID":"-", "Sample ID":"C7", "Factors":{"raw_data_time_format":"High-fat diet"} }, { "Subject ID":"-", "Sample ID":"C8", "Factors":{"raw_data_time_format":"High-fat diet"} }, { "Subject ID":"-", "Sample ID":"C9", "Factors":{"raw_data_time_format":"High-fat diet"} }, { "Subject ID":"-", "Sample ID":"C10", "Factors":{"raw_data_time_format":"High-fat diet"} }, { "Subject ID":"-", "Sample ID":"A1", "Factors":{"raw_data_time_format":"High-fat diet + Antibiotic cocktail"} }, { "Subject ID":"-", "Sample ID":"A2", "Factors":{"raw_data_time_format":"High-fat diet + Antibiotic cocktail"} }, { "Subject ID":"-", "Sample ID":"A3", "Factors":{"raw_data_time_format":"High-fat diet + Antibiotic cocktail"} }, { "Subject ID":"-", "Sample ID":"A4", "Factors":{"raw_data_time_format":"High-fat diet + Antibiotic cocktail"} }, { "Subject ID":"-", "Sample ID":"A5", "Factors":{"raw_data_time_format":"High-fat diet + Antibiotic cocktail"} }, { "Subject ID":"-", "Sample ID":"A6", "Factors":{"raw_data_time_format":"High-fat diet + Antibiotic cocktail"} }, { "Subject ID":"-", "Sample ID":"A7", "Factors":{"raw_data_time_format":"High-fat diet + Antibiotic cocktail"} }, { "Subject ID":"-", "Sample ID":"A8", "Factors":{"raw_data_time_format":"High-fat diet + Antibiotic cocktail"} }, { "Subject ID":"-", "Sample ID":"A9", "Factors":{"raw_data_time_format":"High-fat diet + Antibiotic cocktail"} }, { "Subject ID":"-", "Sample ID":"A10", "Factors":{"raw_data_time_format":"High-fat diet + Antibiotic cocktail"} }, { "Subject ID":"-", "Sample ID":"N1", "Factors":{"raw_data_time_format":"ND"} }, { "Subject ID":"-", "Sample ID":"N2", "Factors":{"raw_data_time_format":"ND"} }, { "Subject ID":"-", "Sample ID":"N3", "Factors":{"raw_data_time_format":"ND"} }, { "Subject ID":"-", "Sample ID":"N4", "Factors":{"raw_data_time_format":"ND"} }, { "Subject ID":"-", "Sample ID":"N5", "Factors":{"raw_data_time_format":"ND"} }, { "Subject ID":"-", "Sample ID":"N6", "Factors":{"raw_data_time_format":"ND"} }, { "Subject ID":"-", "Sample ID":"N7", "Factors":{"raw_data_time_format":"ND"} }, { "Subject ID":"-", "Sample ID":"N8", "Factors":{"raw_data_time_format":"ND"} }, { "Subject ID":"-", "Sample ID":"N9", "Factors":{"raw_data_time_format":"ND"} }, { "Subject ID":"-", "Sample ID":"N10", "Factors":{"raw_data_time_format":"ND"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Liver biopsies from experimental mice were collected and immediately frozen in liquid nitrogen.","SAMPLE_TYPE":"Liver","STORAGE_CONDITIONS":"-80℃"}, "TREATMENT":{"TREATMENT_SUMMARY":"Mice were treated with a normal diet, high-fat high-sugar diet, or treated with a high-fat high-sugar diet and an antibiotic cocktail (ABX)."}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Briefly, liver tissues (50-100 mg) are typically homogenized in the extraction solvent using a bead beater. These samples are then centrifuged at 13,000 g for 15 min to pellet tissue and proteins. The supernatant is combined with water and chloroform, including internal standards, to separate into the polar and non-polar phases.","PROCESSING_STORAGE_CONDITIONS":"-80℃","EXTRACT_STORAGE":"-80℃"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"GC conditions included GC System (Agilent 6890) with column (DB-5MS, 60 m, 0.25 mm ID, 0.25 µm film thickness, J&W Scientific), and gas type helium. MS conditions included MS system (Agilent 5973 MSD) with electron ionization (EI ionization) with scan range from m/z 50 to m/z 650.","CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 6890N","COLUMN_NAME":"Agilent DB5-MS (30m x 0.25mm, 0.25um)"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"INSTRUMENT_NAME":"Agilent 5973","INSTRUMENT_TYPE":"Ion trap","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"The data are deconvoluted using AMDIS and annotated using an in-house constructed spectral library","MS_RESULTS_FILE":"ST002156_AN003531_Results.txt UNITS:None Has m/z:Yes Has RT:Yes RT units:Minutes"} }