{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002166","ANALYSIS_ID":"AN003549","VERSION":"1","CREATED_ON":"November 29, 2021, 3:43 am"}, "PROJECT":{"PROJECT_TITLE":"Untargeted metabolomics following radiation exposure","PROJECT_TYPE":"Untargeted metabolic profiling","PROJECT_SUMMARY":"Comprehensive metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours using LC-MS and NMR.","INSTITUTE":"Institute of Nuclear Medicine and Allied Sciences","DEPARTMENT":"Metabolomics facility","LABORATORY":"Metabolomics facility","LAST_NAME":"Rana","FIRST_NAME":"Poonam","ADDRESS":"DRDO, Lucknow Road, Timarpur, Delhi, Delhi, 110054, India","EMAIL":"poonam@inmas.drdo.in","PHONE":"+911123905319"}, "STUDY":{"STUDY_TITLE":"Untargeted metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours.","STUDY_TYPE":"Untargeted metabolic profiling","STUDY_SUMMARY":"Comprehensive metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours","INSTITUTE":"Institute of Nuclear Medicine and Allied Sciences","LAST_NAME":"Rana","FIRST_NAME":"Poonam","ADDRESS":"DRDO, Lucknow Road, Timarpur, Delhi, Delhi, 110054, India","EMAIL":"poonam@inmas.drdo.in","PHONE":"+911123905319","NUM_GROUPS":"3","TOTAL_SUBJECTS":"21","NUM_MALES":"21"}, "SUBJECT":{"SUBJECT_TYPE":"Other organism","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Male"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"R_Ctrl 1", "Factors":{"Treatment":"Control"} }, { "Subject ID":"-", "Sample ID":"R_Ctrl 2", "Factors":{"Treatment":"Control"} }, { "Subject ID":"-", "Sample ID":"R_Ctrl 3", "Factors":{"Treatment":"Control"} }, { "Subject ID":"-", "Sample ID":"R_Ctrl 4", "Factors":{"Treatment":"Control"} }, { "Subject ID":"-", "Sample ID":"R_Ctrl 5", "Factors":{"Treatment":"Control"} }, { "Subject ID":"-", "Sample ID":"R_Ctrl 6", "Factors":{"Treatment":"Control"} }, { "Subject ID":"-", "Sample ID":"R_Ctrl 7", "Factors":{"Treatment":"Control"} }, { "Subject ID":"-", "Sample ID":"R_5 Gy 1", "Factors":{"Treatment":"5Gy Irradiated"} }, { "Subject ID":"-", "Sample ID":"R_5 Gy 2", "Factors":{"Treatment":"5Gy Irradiated"} }, { "Subject ID":"-", "Sample ID":"R_5 Gy 3", "Factors":{"Treatment":"5Gy Irradiated"} }, { "Subject ID":"-", "Sample ID":"R_5 Gy 4", "Factors":{"Treatment":"5Gy Irradiated"} }, { "Subject ID":"-", "Sample ID":"R_5 Gy 5", "Factors":{"Treatment":"5Gy Irradiated"} }, { "Subject ID":"-", "Sample ID":"R_5 Gy 6", "Factors":{"Treatment":"5Gy Irradiated"} }, { "Subject ID":"-", "Sample ID":"R_5 Gy 7", "Factors":{"Treatment":"5Gy Irradiated"} }, { "Subject ID":"-", "Sample ID":"R_7.5 Gy 1", "Factors":{"Treatment":"7.5Gy Irradiated"} }, { "Subject ID":"-", "Sample ID":"R_7.5 Gy 2", "Factors":{"Treatment":"7.5Gy Irradiated"} }, { "Subject ID":"-", "Sample ID":"R_7.5 Gy 3", "Factors":{"Treatment":"7.5Gy Irradiated"} }, { "Subject ID":"-", "Sample ID":"R_7.5 Gy 4", "Factors":{"Treatment":"7.5Gy Irradiated"} }, { "Subject ID":"-", "Sample ID":"R_7.5 Gy 6", "Factors":{"Treatment":"7.5Gy Irradiated"} }, { "Subject ID":"-", "Sample ID":"R_7.5 Gy 7", "Factors":{"Treatment":"7.5Gy Irradiated"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Animals were acclimatized for 48 hrs in polypropylene cages. Urine samples were collected at 24 hrs post radiation exposure.","SAMPLE_TYPE":"Urine","STORAGE_CONDITIONS":"-80℃","COLLECTION_TUBE_TEMP":"4"}, "TREATMENT":{"TREATMENT_SUMMARY":"Random categorization was done into three groups: control, 5 Gy (sub-lethal) and 7.5 Gy (lethal) irradiated (seven animals in each group). Mice were kept for 12 hrs light and dark cycle with room temperature (19–23°C) and humidity (45–65%) conditions properly maintained. Mice were exposed to total-body gamma radiation (field size; 35x35 cm) of 5 Gy and 7.5 Gy through Tele 60Co unit γ- irradiation facility (Bhabhatron II, Panacea Medical, Bangalore, India) with dose rate of 1 Gy/min. Control mice were sham irradiated.","TREATMENT":"Radiation exposure","TREATMENT_DOSE":"5 Gy and 7.5 Gy Gamma-60Cobalt irradiation"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"150 µl of urine was mixed with 375 µl of deuterated phosphate buffer solution (pH 7.2- 7.4) containing 0.5 mM TSP prepared in D2O. The solution was then transferred to 5 mm NMR tube for data acquisition"}, "ANALYSIS":{"ANALYSIS_TYPE":"NMR"}, "NM":{"INSTRUMENT_NAME":"Bruker","INSTRUMENT_TYPE":"FT-NMR","NMR_EXPERIMENT_TYPE":"1D-1H","SPECTROMETER_FREQUENCY":"600MHz","NMR_RESULTS_FILE":"ST002166_AN003549_Results.txt UNITS:Integral"} }