{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002262","ANALYSIS_ID":"AN003695","VERSION":"1","CREATED_ON":"August 17, 2022, 1:20 pm"},

"PROJECT":{"PROJECT_TITLE":"Mechanistic study of pediatric obesity through metabolomics and metagenomics","PROJECT_SUMMARY":"Pediatric obesity has grown as an important global health problem in the world. Pediatric obesity affects all the organs and it is closely linked to risks of metabolic diseases such as diabetes, cardiovascular disease, and mental disease. Although many researchers reported results about the pediatric obesity study, the mechanism of both pediatric obesity and its treatment remains unclear. Therefore, we investigated the metabolomic pathways related to pediatric obesity and the treatment through metabolomics and metagenomics approaches.","INSTITUTE":"Department of Clinical Pharmacology and Therapeutics, Seoul National University College of Medicine and Hospital","LAST_NAME":"Lee","FIRST_NAME":"Yujin","ADDRESS":"101 Daehak-ro, Jongno-gu, Seoul 110-799, Korea, Seoul, Seoul, 03080, Korea, South","EMAIL":"yoojinlee@snu.ac.kr","PHONE":"+82-10-3380-4686"},

"STUDY":{"STUDY_TITLE":"Metabolomics and metagenomics of pediatric obesity (Feces)","STUDY_SUMMARY":"Pediatric obesity has grown as important global health problem in the world. The pediatric obesity affects all the organs and it is closely linked to risks of metabolic diseases such as diabetes, cardiovascular disease, and mental disease. However, the mechanism of both the pediatric obesity and treatment of the obesity remains unclear. Therefore, we investigated metabolomic pathways related to the pediatric obesity and the treatment through metabolomics and metagenomics approaches.","INSTITUTE":"Department of Clinical Pharmacology and Therapeutics, Seoul National University College of Medicine and Hospital","LAST_NAME":"Lee","FIRST_NAME":"Yujin","ADDRESS":"101 Daehak-ro, Jongno-gu, Seoul 110-799, Korea, Seoul, Seoul, 03080, Korea, South","EMAIL":"yoojinlee@snu.ac.kr","PHONE":"+82-10-3380-4686"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","GENDER":"Male and female"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"Nor01",
"Sample ID":"N01",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N01_01"}
},
{
"Subject ID":"Nor02",
"Sample ID":"N02",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N02_01"}
},
{
"Subject ID":"Nor03",
"Sample ID":"N03",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N03_01"}
},
{
"Subject ID":"Nor04",
"Sample ID":"N04",
"Factors":{"Gender":"F","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N04_01"}
},
{
"Subject ID":"Nor05",
"Sample ID":"N05",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N05_01"}
},
{
"Subject ID":"Nor06",
"Sample ID":"N06",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N06_01"}
},
{
"Subject ID":"Nor07",
"Sample ID":"N07",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N07_01"}
},
{
"Subject ID":"Nor08",
"Sample ID":"N08",
"Factors":{"Gender":"F","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N08_01"}
},
{
"Subject ID":"Nor09",
"Sample ID":"N09",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N09_01"}
},
{
"Subject ID":"Nor10",
"Sample ID":"N10",
"Factors":{"Gender":"F","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N10_01"}
},
{
"Subject ID":"Nor11",
"Sample ID":"N11",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N11_01"}
},
{
"Subject ID":"Nor12",
"Sample ID":"N12",
"Factors":{"Gender":"F","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N12_01"}
},
{
"Subject ID":"Nor13",
"Sample ID":"N13",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N13_01"}
},
{
"Subject ID":"Nor14",
"Sample ID":"N14",
"Factors":{"Gender":"F","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N14_01"}
},
{
"Subject ID":"Nor15",
"Sample ID":"N15",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N15_01"}
},
{
"Subject ID":"Nor16",
"Sample ID":"N16",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N16_01"}
},
{
"Subject ID":"Nor17",
"Sample ID":"N17",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N17_01"}
},
{
"Subject ID":"Nor18",
"Sample ID":"N18",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N18_01"}
},
{
"Subject ID":"Nor19",
"Sample ID":"N19",
"Factors":{"Gender":"F","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N19_01"}
},
{
"Subject ID":"Nor20",
"Sample ID":"N20",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N20_01"}
},
{
"Subject ID":"Nor21",
"Sample ID":"N21",
"Factors":{"Gender":"M","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N21_01"}
},
{
"Subject ID":"Nor22",
"Sample ID":"N22",
"Factors":{"Gender":"F","Group":"Normal"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"N22_01"}
},
{
"Subject ID":"Obe01",
"Sample ID":"M01",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M01_01"}
},
{
"Subject ID":"Obe02",
"Sample ID":"M02",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M02_01"}
},
{
"Subject ID":"Obe03",
"Sample ID":"M03",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M03_01"}
},
{
"Subject ID":"Obe04",
"Sample ID":"M04",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M04_01"}
},
{
"Subject ID":"Obe05",
"Sample ID":"M05",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M05_01"}
},
{
"Subject ID":"Obe06",
"Sample ID":"M06",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M06_01"}
},
{
"Subject ID":"Obe07",
"Sample ID":"M07",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M07_01"}
},
{
"Subject ID":"Obe08",
"Sample ID":"M08",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M08_01"}
},
{
"Subject ID":"Obe09",
"Sample ID":"M09",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M09_01"}
},
{
"Subject ID":"Obe10",
"Sample ID":"M10",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M10_01"}
},
{
"Subject ID":"Obe11",
"Sample ID":"M11",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M11_01"}
},
{
"Subject ID":"Obe12",
"Sample ID":"M12",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M12_01"}
},
{
"Subject ID":"Obe14",
"Sample ID":"M14",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M14_01"}
},
{
"Subject ID":"Obe15",
"Sample ID":"M15",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M15_01"}
},
{
"Subject ID":"Obe16",
"Sample ID":"M16",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M16_01"}
},
{
"Subject ID":"Obe17",
"Sample ID":"M17",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M17_01"}
},
{
"Subject ID":"Obe18",
"Sample ID":"M18",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M18_01"}
},
{
"Subject ID":"Obe19",
"Sample ID":"M19",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M19_01"}
},
{
"Subject ID":"Obe20",
"Sample ID":"M20",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M20_01"}
},
{
"Subject ID":"Obe21",
"Sample ID":"M21",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M21_01"}
},
{
"Subject ID":"Obe22",
"Sample ID":"M22",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M22_01"}
},
{
"Subject ID":"Obe23",
"Sample ID":"M23",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M23_01"}
},
{
"Subject ID":"Obe24",
"Sample ID":"M24",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M24_01"}
},
{
"Subject ID":"Obe25",
"Sample ID":"M25",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M25_01"}
},
{
"Subject ID":"Obe26",
"Sample ID":"M26",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M26_01"}
},
{
"Subject ID":"Obe28",
"Sample ID":"M28",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M28_01"}
},
{
"Subject ID":"Obe29",
"Sample ID":"M29",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M29_01"}
},
{
"Subject ID":"Obe31",
"Sample ID":"M31",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M31_01"}
},
{
"Subject ID":"Obe32",
"Sample ID":"M32",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M32_01"}
},
{
"Subject ID":"Obe33",
"Sample ID":"M33",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M33_01"}
},
{
"Subject ID":"Obe35",
"Sample ID":"M35",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M35_01"}
},
{
"Subject ID":"Obe36",
"Sample ID":"M36",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M36_01"}
},
{
"Subject ID":"Obe37",
"Sample ID":"M37",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M37_01"}
},
{
"Subject ID":"Obe40",
"Sample ID":"M40",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M40_01"}
},
{
"Subject ID":"Obe41",
"Sample ID":"M41",
"Factors":{"Gender":"F","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M41_01"}
},
{
"Subject ID":"Obe42",
"Sample ID":"M42",
"Factors":{"Gender":"M","Group":"Obe"},
"Additional sample data":{"Race":"Asian","RAW_FILE_NAME":"M42_01"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"This longitudinal cohort study is an analysis of fecal samples collected from obese children before and after a 2-month weight reduction program that consisted of three visits to the hospital.","SAMPLE_TYPE":"Feces"},

"TREATMENT":{"TREATMENT_SUMMARY":"Participants completed the questionnaires on general lifestyle (the time spent studying and using electronic devices, the duration and frequency of regular exercise, the presence of easily accessible locations to exercise, and their mode of transportation to school) and eating habits (meal duration, the consumption of late-night snacks, the consumption of breakfast, and the intake of sugar-sweetened beverages) and submitted them at the first hospital visit."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Frozen serum samples were thawed on ice. For preparation of the serum, a 50 µL sample was extracted using 1 mL of N2-degassed 1st extraction solution. Then, the samples were mixed for 10 min and centrifuged for 10 min at 18945 RCF and 4°C. The supernatant was dried for 6 hours at 45°C. The dried samples were re-extracted with 2nd extraction solution. Then, the extracted samples were redried for 8 hours under the same conditions used in the first extraction step. The dried samples were derivatized with methoxyamine at 30°C for 90 min and subsequently trimethylsilylated with a mixture of fatty acid methyl ester, which is used for the retention time index, in N-methyl-N-(trimethylsilyl)-trifluoroacetamide at 70°C for 45 min."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890B","COLUMN_NAME":"Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Leco Pegasus HT TOF","INSTRUMENT_TYPE":"GC-TOF","MS_TYPE":"EI","ION_MODE":"NEGATIVE","MS_COMMENTS":"Leco ChromaTOF-GC Sofrware v4.72.0.0"},

"MS_METABOLITE_DATA":{
"Units":"intensity",

"Data":[{"Metabolite":"Myristic acid","N01":"0.33232628","N02":"0.65053813","N03":"0.26737895","N04":"0.44766602","N05":"1.08269083","N06":"1.35460689","N07":"0.37312625","N08":"4.25685923","N09":"0.51136583","N10":"0.55490879","N11":"0.3322308","N12":"0.17790085","N13":"0.42789386","N14":"0.58843711","N15":"0.16218007","N16":"0.27568284","N17":"0.78389789","N18":"0.59835113","N19":"0.49801741","N20":"0.5708189","N21":"0.11547569","N22":"0.78443123","M01":"0.37316149","M02":"0.08589866","M03":"0.31244707","M04":"0.10753602","M05":"0.10088269","M06":"0.09632624","M07":"0.20700884","M08":"0.44047976","M09":"0.0907977","M10":"0.51608023","M11":"0.51051881","M12":"0.24627744","M14":"0.09715929","M15":"0.14975823","M16":"0.23791886","M17":"0.19840668","M18":"0.17287478","M19":"0.50533363","M20":"0.48960397","M21":"0.220086","M22":"0.11197247","M23":"0.10910955","M24":"0.19602252","M25":"0.21652516","M26":"0.57995067","M28":"0.62417328","M29":"0.54745668","M31":"0.15821929","M32":"0.11251408","M33":"0.14976544","M35":"0.44981869","M36":"2.1620622","M37":"0.24273371","M40":"0.3656503","M41":"0.21454016","M42":"0.12286158"},{"Metabolite":"Putrescine","N01":"2.01849484","N02":"0.46398475","N03":"5.01795886","N04":"3.81626598","N05":"0.99508756","N06":"5.91270317","N07":"1.31866832","N08":"1.0060295","N09":"0.11446259","N10":"0.87715883","N11":"0.14644466","N12":"0.37495555","N13":"0.40501624","N14":"0.03403395","N15":"5.53343447","N16":"0.39071707","N17":"3.38676419","N18":"2.33536062","N19":"1.73271646","N20":"0.26348322","N21":"0.09677443","N22":"0.30838436","M01":"1.26924359","M02":"6.36760181","M03":"8.11475919","M04":"4.35561034","M05":"9.33303126","M06":"0.25958122","M07":"2.48408765","M08":"2.28035172","M09":"9.14586957","M10":"0.06985136","M11":"8.07574152","M12":"0.21998109","M14":"12.31358907","M15":"17.69118121","M16":"3.09975549","M17":"1.5815429","M18":"18.21359765","M19":"0.49923376","M20":"2.35379863","M21":"2.88659795","M22":"0.22364466","M23":"3.14077826","M24":"8.66396881","M25":"4.5166656","M26":"14.33041944","M28":"0.04502455","M29":"0.88072302","M31":"62.11823685","M32":"28.48781037","M33":"0.94381456","M35":"4.27327776","M36":"1.23914381","M37":"1.37791189","M40":"1.74884801","M41":"0.54174753","M42":"2.10379293"}],

"Metabolites":[{"Metabolite":"Myristic acid","HMDB":"HMDB0000806","PubChem":"11005","KEGG":"C06424"},{"Metabolite":"Putrescine","HMDB":"HMDB0001414","PubChem":"1045","KEGG":"C00134"}]
}

}