{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002406","ANALYSIS_ID":"AN003923","VERSION":"1","CREATED_ON":"December 12, 2022, 12:48 pm"},

"PROJECT":{"PROJECT_TITLE":"Silicon ameliorates clubroot responses in canola (Brassica napus): A “multi-omics”-based investigation into possible mechanisms","PROJECT_TYPE":"MS quantitative analysis","PROJECT_SUMMARY":"Clubroot disease, caused by Plasmodiophora brassicae Woronin results in severe yield losses in Brassica crops, including canola. Silicon (Si) mitigates several stresses and enhances plant resistance to phytopathogens. We investigated the effects of Si on clubroot disease symptoms in canola at two concentrations of Si (Si1.0 and Si0.5). In addition, the effects of Si on P. brassicae-induced gene expression, endogenous levels of phytohormones and metabolites were also studied. Si application reduced clubroot symptoms and improved plant growth under greenhouse conditions. Pathogen-induced transcript-level changes were affected by Si treatment to P. brassicae with genes related to antioxidant activity, phytohormone biosynthesis and signalling, nitrogen metabolism and secondary metabolism exhibiting differential expression. Endogenous levels of several phytohormones (e.g., auxin, cytokinin, salicylic acid and abscisic acid), amino acids and secondary metabolites (e.g., glucosinolates) were affected by Si. This is the first report that Si ameliorates clubroot symptoms and its possible mode of action.","INSTITUTE":"Trent University","DEPARTMENT":"Biology","LABORATORY":"Emery Lab","LAST_NAME":"Kisiala","FIRST_NAME":"Anna","ADDRESS":"1600 West Bank Drive, Trent University, Peterborough, ON, K9L 0G2, Canada","EMAIL":"annakisiala@trentu.ca","PHONE":"7057481011","FUNDING_SOURCE":"Alberta Agriculture and Forestry","CONTRIBUTORS":"Ananya Sarkar, Anna Kisiala, Dinesh Adhikary, Urmila Basu, Neil Emery, Rahman, Nat N. V. Kav"},

"STUDY":{"STUDY_TITLE":"Silicon ameliorates clubroot responses in canola (Brassica napus): A “multi-omics”-based investigation into possible mechanisms","STUDY_TYPE":"Timecourse experiment","STUDY_SUMMARY":"Clubroot disease, caused by Plasmodiophora brassicae Woronin results in severe yield losses in Brassica crops, including canola. Silicon (Si) mitigates several stresses and enhances plant resistance to phytopathogens. We investigated the effects of Si on clubroot disease symptoms in canola at two concentrations of Si (Si1.0 and Si0.5). In addition, the effects of Si on P. brassicae-induced gene expression, endogenous levels of phytohormones and metabolites were also studied. Si application reduced clubroot symptoms and improved plant growth under greenhouse conditions. Pathogen-induced transcript-level changes were affected by Si treatment to P. brassicae with genes related to antioxidant activity, phytohormone biosynthesis and signalling, nitrogen metabolism and secondary metabolism exhibiting differential expression. Endogenous levels of several phytohormones (e.g., auxin, cytokinin, salicylic acid and abscisic acid), amino acids and secondary metabolites (e.g., glucosinolates) were affected by Si. This is the first report that Si ameliorates clubroot symptoms and its possible mode of action.","INSTITUTE":"Trent University","DEPARTMENT":"Biology","LABORATORY":"Emery Lab","LAST_NAME":"Kisiala","FIRST_NAME":"Anna","ADDRESS":"1600 West Bank Drive, Trent University, Peterborough, ON, K9L 0G2, Canada","EMAIL":"annakisiala@trentu.ca","PHONE":"7057481011"},

"SUBJECT":{"SUBJECT_TYPE":"Plant","SUBJECT_SPECIES":"Brassica napus","TAXONOMY_ID":"3708"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"C18-1-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C1-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-1-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C1-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-1-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H1-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-1-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H1-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-2-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C2-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-2-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C2-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-2-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H2-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-2-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H2-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-3-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C3-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-3-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C3-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-3-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H3-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-3-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H3-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-4-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C4-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-4-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C4-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-4-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H4-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-4-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H4-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-5-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C5-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-5-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C5-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-5-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H5-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-5-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H5-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-6-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C6-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-6-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C6-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-6-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H6-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-6-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H6-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-7-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C7-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-7-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C7-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-7-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H7-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-7-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H7-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-8-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C8-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-8-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C8-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-8-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H8-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-8-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H8-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-9-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C9-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-9-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C9-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-9-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H9-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-9-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H9-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-10-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C10-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-10-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C10-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-10-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H10-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-10-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H10-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-11-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C11-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-11-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C11-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-11-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H11-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-11-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H11-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-12-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C12-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-12-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C12-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-12-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H12-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-12-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H12-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-13-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C13-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-13-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C13-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-13-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H13-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-13-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H13-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-14-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C14-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-14-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C14-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-14-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H14-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-14-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H14-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-15-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C15-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-15-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C15-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-15-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H15-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-15-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H15-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-16-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C16-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-16-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C16-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-16-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H16-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-16-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H16-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-17-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C17-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-17-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C17-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-17-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H17-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-17-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H17-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-18-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C18-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-18-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C18-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-18-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H18-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-18-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H18-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-19-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C19-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-19-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C19-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-19-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H19-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-19-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H19-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-20-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C20-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-20-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C20-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-20-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H20-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-20-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H20-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-21-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C21-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-21-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C21-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-21-pos",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H21-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-21-neg",
"Factors":{"Developmental stage (DPI)":"day 14","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H21-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-22-pos",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C22-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-22-neg",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C22-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-22-pos",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H22-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-22-neg",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H22-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-23-pos",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C23-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-23-neg",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C23-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-23-pos",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H23-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-23-neg",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H23-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-24-pos",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C24-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-24-neg",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C24-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-24-pos",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H24-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-24-neg",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H24-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-25-pos",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C25-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-25-neg",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C25-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-25-pos",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H25-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-25-neg",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H25-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-26-pos",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C26-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-26-neg",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C26-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-26-pos",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H26-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-26-neg",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"0.5"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H26-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-27-pos",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C27-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-27-neg",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"C27-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-27-pos",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H27-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-27-neg",
"Factors":{"Developmental stage (DPI)":"day 21","Silicon treatment [g]":"1.0"},
"Additional sample data":{"Organism":"Brassica napus","Cultivar":"Canola (HiQ)","Phenotype":"susceptible to Plasmodiophora brassicae (P3H)","Tissue":"roots","RAW_FILE_NAME":"H27-neg.mzXML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"A total of 27 samples from the three treatments (PC, Si0.5 and Si1.0) were used for metabolomic analyses at 7-, 14- and 21 dpi. Hi-Q seedlings were grown in the soil-mix as previously described with/without Si amendment, inoculated with P. brassicae, and subsequently, washed thoroughly, flash-frozen in liquid nitrogen and stored at -80°C until analysis. Each biological replicate was comprised of 5 individual plants per treatment, and three biological replicates were generated from three independent experiments. A total of 27 samples were generated for transcriptomics and metabolomics analyses [3 treatments (PC, Si0.5 and Si1.0) x 3 time-points (7-, 14- and 21 dpi) x 3 biological replicates].","COLLECTION_PROTOCOL_FILENAME":"Collection_protocol","SAMPLE_TYPE":"Plant","COLLECTION_LOCATION":"Alberta, Canada","COLLECTION_FREQUENCY":"7, 14 21 DPI","VOLUMEORAMOUNT_COLLECTED":"0.07g per sample","STORAGE_CONDITIONS":"-80℃","COLLECTION_VIALS":"2 mL round-bottom Eppendorf tubes","STORAGE_VIALS":"2 mL round-bottom Eppendorf tubes","COLLECTION_TUBE_TEMP":"-20"},

"TREATMENT":{"TREATMENT_SUMMARY":"Sodium silicate (Na2SiO3, Mol. Wt: 122.06 g/L), obtained from Sigma-Aldrich (St. Louis, US), was mixed with 100 g Sunshine Professional Growing Mix (Sun Gro Horticulture, Seba Beach, Canada) and the following mixtures (treatments) were produced: 0.0 g Si (control), 0.1 g Si (Si:soil in 1:1000, wt/wt), 0.25 g (Si:soil in 1:400, wt/wt), 0.5 g (Si:soil in 1:200, wt/wt), 0.75 g (Si:soil in 3:400, wt/wt) and 1.0 g (Si:soil in 1:100, wt/wt). These treatments, hereafter, reported as Si0.0 or PC, Si0.1, Si0.25, Si0.5, Si0.75 and Si1.0, respectively. After mixing thoroughly, the pH of the soil mix was determined prior to seeding. A clubroot susceptible spring canola (Brassica napus L.) cultivar Hi-Q, developed at the University of Alberta, was used in this study. The plants were grown under greenhouse conditions (22/19°C and 16/8h photoperiod) on the above-mentioned six soil media (treatments) containing varying concentration of Si. A total of 30 plants, from three replications of each treatment (i.e. 10 plants per replication) were used. A single spore suspension of P. brassicae pathotype 3, classified as pathotype 3H (P3H) under the Canadian Clubroot Differential (CCD) set, was prepared by homogenizing the clubroot galls, and used to inoculate the seedlings following previously described procedures."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"A total of 27 samples from the three treatments (PC, Si0.5 and Si1.0) were used for metabolomic analyses at 7-, 14- and 21 dpi. Hi-Q seedlings were grown in the soil-mix as previously described with/without Si amendment, inoculated with P. brassicae, and subsequently, washed thoroughly, flash-frozen in liquid nitrogen and stored at -80°C until analysis. Each biological replicate was comprised of 5 individual plants per treatment, and three biological replicates were generated from three independent experiments. A total of 27 samples were generated for transcriptomics and metabolomics analyses [3 treatments (PC, Si0.5 and Si1.0) x 3 time-points (7-, 14- and 21 dpi) x 3 biological replicates].","SAMPLEPREP_PROTOCOL_FILENAME":"Sample Prep protocol","PROCESSING_STORAGE_CONDITIONS":"-20℃","EXTRACTION_METHOD":"50% ACN","EXTRACT_CLEANUP":"HLB SPE","EXTRACT_STORAGE":"-20℃","SAMPLE_RESUSPENSION":"Primary metabolites - 90% ACN, glucosinolates - 5% ACN","SAMPLE_SPIKING":"Primary metabolites - Labeled amino acid mix; Glucosinolates - labeled CK mix"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"Phenomonex Kinetex C18 (50 x 2.1mm, 2.6um)","SOLVENT_A":"100% water; 0.08% acetic acid","SOLVENT_B":"100% acetonitrile; 0.08% acetic acid","FLOW_GRADIENT":"Mobile phase 100% A held for 1.25 min, then decreased to 50% over 1.75 min and to 0% over the next 0.5 min, held at 0% for 2 min and returned to 100% over 0.5 min for 4 min of column re-equilibration.","FLOW_RATE":"0.3 ml/min","COLUMN_TEMPERATURE":"RT","INJECTION_TEMPERATURE":"4"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"Processing of the full scan and ddMS2 data was conducted using the Xcalibur 4.1 software (Thermo Scientific, Waltham, US). Metabolites were identified by accurate mass, comparison of retention times to authentic standards or by accurate mass and comparison of fragmentation patterns to MS/MS databases (METLIN, PubChem)."},

"MS_METABOLITE_DATA":{
"Units":"normalized relative level",

"Data":[{"Metabolite":"1,1'-diindolylmethane","C18-1-neg":"1.019","C18-2-neg":"0.948","C18-3-neg":"1.002","C18-4-neg":"1.413","C18-5-neg":"1.102","C18-6-neg":"1.006","C18-7-neg":"0.620","C18-8-neg":"0.991","C18-9-neg":"0.901","C18-10-neg":"0.798","C18-11-neg":"0.748","C18-12-neg":"0.535","C18-13-neg":"0.891","C18-14-neg":"1.003","C18-15-neg":"0.985","C18-16-neg":"0.982","C18-17-neg":"0.661","C18-18-neg":"0.833","C18-19-neg":"0.760","C18-20-neg":"0.716","C18-21-neg":"0.696","C18-22-neg":"0.880","C18-23-neg":"0.279","C18-24-neg":"0.054","C18-25-neg":"1.106","C18-26-neg":"0.919","C18-27-neg":"1.025"},{"Metabolite":"4-methoxyglucobrassicin","C18-1-neg":"0.244","C18-2-neg":"0.045","C18-3-neg":"0.054","C18-4-neg":"0.089","C18-5-neg":"0.406","C18-6-neg":"0.186","C18-7-neg":"0.334","C18-8-neg":"0.210","C18-9-neg":"0.066","C18-10-neg":"0.452","C18-11-neg":"1.351","C18-12-neg":"0.763","C18-13-neg":"0.248","C18-14-neg":"0.222","C18-15-neg":"0.124","C18-16-neg":"0.049","C18-17-neg":"0.042","C18-18-neg":"0.066","C18-19-neg":"1.324","C18-20-neg":"1.223","C18-21-neg":"0.544","C18-22-neg":"0.128","C18-23-neg":"0.128","C18-24-neg":"0.151","C18-25-neg":"0.081","C18-26-neg":"0.228","C18-27-neg":"0.108"},{"Metabolite":"Brassicanal 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}

}