{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002446","ANALYSIS_ID":"AN003985","VERSION":"1","CREATED_ON":"09-01-2023"},

"PROJECT":{"PROJECT_TITLE":"Reprogramming of miR-142-dependent Metabolism Drives Blast Transformation of Chronic Myelogenous Leukemia","PROJECT_TYPE":"MS quantitative analysis","PROJECT_SUMMARY":"Metabolic profiling of miR-142+/+BCR-ABL Leukemic stem cell (LSC)-enriched Lineage-Sca-1+c-Kit+ cells (LSKs) compared to miR-142-/-BCR-ABL controls","INSTITUTE":"Translational Genomics Research Institute","LAST_NAME":"Mansfield","FIRST_NAME":"Krystine","ADDRESS":"445 N 5th St, Phoenix, AZ, 85004, USA","EMAIL":"kgarcia@tgen.org","PHONE":"602-343-8832","PUBLICATIONS":"https://www.nature.com/articles/s41467-023-41167-z","DOI":"http://dx.doi.org/10.21228/M8VD8T"},

"STUDY":{"STUDY_TITLE":"Untargeted metabolomics of miR-142 WT vs KO CML cells","STUDY_SUMMARY":"MiR-142 is dynamically expressed and plays a regulatory role in hematopoiesis. Based on the simple observation that miR-142 levels are significantly lower in CD34+CD38- cells from blast crisis (BC) chronic myeloid leukemia (CML). CML patients compared with chronic phase (CP) CML patients (p=0.002), we hypothesized that miR-142 deficit plays a role in BC transformation. To test this hypothesis, we generated a miR-142 KO BCR-ABL (i.e., miR-142−/−BCR-ABL) mouse by crossing a miR-142−/− mouse with a miR-142+/+BCR-ABL mouse. While the miR-142+/+BCR-ABL mice developed and died of CP CML, the miR-142−/−BCR-ABL mice developed a BC-like phenotype in the absence of any other acquired gene mutations and died significantly sooner than miR-142+/+BCR-ABL CP controls (p=0.001). Leukemic stem cell (LSC)-enriched Lineage-Sca-1+c-Kit+ cells (LSKs) from diseased miR-142−/−BCR-ABL mice transplanted into congenic recipients, recapitulated the BC features thereby suggesting stable transformation of CP-LSCs into BC-LSCs in the miR-142 KO CML mouse. Single cell (sc) RNA-seq profiling showed that miR-142 deficit changed the cellular landscape of the miR-142−/−BCR-ABL LSKs compared with miR-142+/+BCR-ABL LSKs with expansion of myeloid-primed and loss of lymphoid-primed factions. Bulk RNA-seq analyses along with unbiased metabolomic profiling and functional metabolic assays demonstrated enhanced fatty acid β-oxidation (FAO) and oxidative phosphorylation (OxPhos) in miR-142−/−BCR-ABL LSKs vs miR-142+/+BCR-ABL LSKs. MiR-142 deficit enhanced FAO in miR-142−/−BCR-ABL LSKs by increasing the expression of CPT1A and CPT1B, that controls the cytosol-to-mitochondrial acyl-carnitine transport, a critical step in FAO. MiR-142 deficit also enhanced OxPhos in miR-142−/−BCR-ABL LSKs by increasing mitochondrial fusion and activity. As the homeostasis and activity of LSCs depend on higher levels of these oxidative metabolism processes, we then postulate that miR-142 deficit is a potentially druggable target for BC-LSCs. To this end, we developed a novel CpG-miR-142 mimic oligonucleotide (ODN; i.e., CpG-M-miR-142) that corrected the miR-142 deficit and alone or in combination with a tyrosine kinase inhibitor (TKI) significantly reduced LSC burden and prolonged survival of miR-142−/−BCR-ABL mice. The results from murine models were validated in BC CD34+CD38- primary blasts and patient-derived xenografts (PDXs). In conclusion, an acquired miR-142 deficit sufficed in transforming CP-LSCs into BC-LSCs, via enhancement of bioenergetic oxidative metabolism in absence of any additional gene mutations, and likely represent a novel therapeutic target in BC CML.","INSTITUTE":"Translational Genomics Research Institute","LAST_NAME":"Mansfield","FIRST_NAME":"Krystine","ADDRESS":"445 N 5th St, Phoenix, AZ, 85004, USA","EMAIL":"kgarcia@tgen.org","PHONE":"602-343-8832","SUBMIT_DATE":"2023-01-13"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Male and female"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"A09xKOxFx01",
"Sample ID":"Norm. Area: 088_0003_02B_R_A09xKOxFx01xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F9)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A09xKOxFx01xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A10xKOxFx02",
"Sample ID":"Norm. Area: 088_0003_02B_R_A10xKOxFx02xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F10)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A10xKOxFx02xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A11xKOxFx03",
"Sample ID":"Norm. Area: 088_0003_02B_R_A11xKOxFx03xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F11)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A11xKOxFx03xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A12xKOxFx04",
"Sample ID":"Norm. Area: 088_0003_02B_R_A12xKOxFx04xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F12)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A12xKOxFx04xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A13xKOxFx05",
"Sample ID":"Norm. Area: 088_0003_02B_R_A13xKOxFx05xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F13)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A13xKOxFx05xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A14xKOxMx01",
"Sample ID":"Norm. Area: 088_0003_02B_R_A14xKOxMx01xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F14)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A14xKOxMx01xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A15xKOxMx02",
"Sample ID":"Norm. Area: 088_0003_02B_R_A15xKOxMx02xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F15)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A15xKOxMx02xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A16xKOxMx03",
"Sample ID":"Norm. Area: 088_0003_02B_R_A16xKOxMx03xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F16)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A16xKOxMx03xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A17xKOxMx04",
"Sample ID":"Norm. Area: 088_0003_02B_R_A17xKOxMx04xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F17)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A17xKOxMx04xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A18xKOxMx05",
"Sample ID":"Norm. Area: 088_0003_02B_R_A18xKOxMx05xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F18)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A18xKOxMx05xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A09xKOxFx01",
"Sample ID":"Norm. Area: 088_0003_02D_R_A09xKOxFx01xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F175)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A09xKOxFx01xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A10xKOxFx02",
"Sample ID":"Norm. Area: 088_0003_02D_R_A10xKOxFx02xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F176)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A10xKOxFx02xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A11xKOxFx03",
"Sample ID":"Norm. Area: 088_0003_02D_R_A11xKOxFx03xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F177)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A11xKOxFx03xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A12xKOxFx04",
"Sample ID":"Norm. Area: 088_0003_02D_R_A12xKOxFx04xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F178)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A12xKOxFx04xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A13xKOxFx05",
"Sample ID":"Norm. Area: 088_0003_02D_R_A13xKOxFx05xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F179)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A13xKOxFx05xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A14xKOxMx01",
"Sample ID":"Norm. Area: 088_0003_02D_R_A14xKOxMx01xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F180)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A14xKOxMx01xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A15xKOxMx02",
"Sample ID":"Norm. Area: 088_0003_02D_R_A15xKOxMx02xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F181)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A15xKOxMx02xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A16xKOxMx03",
"Sample ID":"Norm. Area: 088_0003_02D_R_A16xKOxMx03xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F182)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A16xKOxMx03xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A17xKOxMx04",
"Sample ID":"Norm. Area: 088_0003_02D_R_A17xKOxMx04xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F183)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A17xKOxMx04xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A18xKOxMx05",
"Sample ID":"Norm. Area: 088_0003_02D_R_A18xKOxMx05xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F184)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A18xKOxMx05xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A09xKOxFx01",
"Sample ID":"Norm. Area: 088_0003_02E_R_A09xKOxFx01xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F206)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A09xKOxFx01xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A10xKOxFx02",
"Sample ID":"Norm. Area: 088_0003_02E_R_A10xKOxFx02xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F207)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A10xKOxFx02xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A11xKOxFx03",
"Sample ID":"Norm. Area: 088_0003_02E_R_A11xKOxFx03xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F208)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A11xKOxFx03xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A12xKOxFx04",
"Sample ID":"Norm. Area: 088_0003_02E_R_A12xKOxFx04xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F209)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A12xKOxFx04xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A13xKOxFx05",
"Sample ID":"Norm. Area: 088_0003_02E_R_A13xKOxFx05xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F210)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A13xKOxFx05xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A14xKOxMx01",
"Sample ID":"Norm. Area: 088_0003_02E_R_A14xKOxMx01xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F211)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A14xKOxMx01xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A15xKOxMx02",
"Sample ID":"Norm. Area: 088_0003_02E_R_A15xKOxMx02xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F212)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A15xKOxMx02xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A16xKOxMx03",
"Sample ID":"Norm. Area: 088_0003_02E_R_A16xKOxMx03xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F213)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A16xKOxMx03xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A17xKOxMx04",
"Sample ID":"Norm. Area: 088_0003_02E_R_A17xKOxMx04xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F214)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A17xKOxMx04xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A18xKOxMx05",
"Sample ID":"Norm. Area: 088_0003_02E_R_A18xKOxMx05xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F215)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A18xKOxMx05xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A09xKOxFx01",
"Sample ID":"Norm. Area: 088_0003_02G_R_A09xKOxFx01conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F145)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A09xKOxFx01conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A10xKOxFx02",
"Sample ID":"Norm. Area: 088_0003_02G_R_A10xKOxFx02conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F146)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A10xKOxFx02conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A11xKOxFx03",
"Sample ID":"Norm. Area: 088_0003_02G_R_A11xKOxFx03conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F147)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A11xKOxFx03conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A12xKOxFx04",
"Sample ID":"Norm. Area: 088_0003_02G_R_A12xKOxFx04conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F148)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A12xKOxFx04conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A13xKOxFx05",
"Sample ID":"Norm. Area: 088_0003_02G_R_A13xKOxFx05conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F149)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A13xKOxFx05conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A14xKOxMx01",
"Sample ID":"Norm. Area: 088_0003_02G_R_A14xKOxMx01conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F150)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A14xKOxMx01conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A15xKOxMx02",
"Sample ID":"Norm. Area: 088_0003_02G_R_A15xKOxMx02conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F151)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A15xKOxMx02conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A16xKOxMx03",
"Sample ID":"Norm. Area: 088_0003_02G_R_A16xKOxMx03conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F152)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A16xKOxMx03conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A17xKOxMx04",
"Sample ID":"Norm. Area: 088_0003_02G_R_A17xKOxMx04conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F153)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A17xKOxMx04conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A18xKOxMx05",
"Sample ID":"Norm. Area: 088_0003_02G_R_A18xKOxMx05conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F154)",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A18xKOxMx05conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A01xWTxFx01",
"Sample ID":"Norm. Area: 088_0003_02B_R_A01xWTxFx01xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F1)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A01xWTxFx01xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A02xWTxFx02",
"Sample ID":"Norm. Area: 088_0003_02B_R_A02xWTxFx02xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F2)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A02xWTxFx02xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A03xWTxFx03",
"Sample ID":"Norm. Area: 088_0003_02B_R_A03xWTxFx03xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F3)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A03xWTxFx03xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A04xWTxFx04",
"Sample ID":"Norm. Area: 088_0003_02B_R_A04xWTxFx04xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F4)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A04xWTxFx04xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A05xWTxMx01",
"Sample ID":"Norm. Area: 088_0003_02B_R_A05xWTxMx01xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F5)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A05xWTxMx01xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A06xWTxMx02",
"Sample ID":"Norm. Area: 088_0003_02B_R_A06xWTxMx02xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F6)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A06xWTxMx02xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A07xWTxMx03",
"Sample ID":"Norm. Area: 088_0003_02B_R_A07xWTxMx03xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F7)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A07xWTxMx03xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A08xWTxMx04",
"Sample ID":"Norm. Area: 088_0003_02B_R_A08xWTxMx04xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F8)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02B_R_A08xWTxMx04xHILICpos_09SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A01xWTxFx01",
"Sample ID":"Norm. Area: 088_0003_02D_R_A01xWTxFx01xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F167)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A01xWTxFx01xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A02xWTxFx02",
"Sample ID":"Norm. Area: 088_0003_02D_R_A02xWTxFx02xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F168)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A02xWTxFx02xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A03xWTxFx03",
"Sample ID":"Norm. Area: 088_0003_02D_R_A03xWTxFx03xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F169)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A03xWTxFx03xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A04xWTxFx04",
"Sample ID":"Norm. Area: 088_0003_02D_R_A04xWTxFx04xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F170)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A04xWTxFx04xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A05xWTxMx01",
"Sample ID":"Norm. Area: 088_0003_02D_R_A05xWTxMx01xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F171)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A05xWTxMx01xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A06xWTxMx02",
"Sample ID":"Norm. Area: 088_0003_02D_R_A06xWTxMx02xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F172)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A06xWTxMx02xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A07xWTxMx03",
"Sample ID":"Norm. Area: 088_0003_02D_R_A07xWTxMx03xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F173)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A07xWTxMx03xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A08xWTxMx04",
"Sample ID":"Norm. Area: 088_0003_02D_R_A08xWTxMx04xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F174)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02D_R_A08xWTxMx04xconcRPpos_21SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A01xWTxFx01",
"Sample ID":"Norm. Area: 088_0003_02E_R_A01xWTxFx01xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F198)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A01xWTxFx01xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A02xWTxFx02",
"Sample ID":"Norm. Area: 088_0003_02E_R_A02xWTxFx02xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F199)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A02xWTxFx02xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A03xWTxFx03",
"Sample ID":"Norm. Area: 088_0003_02E_R_A03xWTxFx03xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F200)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A03xWTxFx03xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A04xWTxFx04",
"Sample ID":"Norm. Area: 088_0003_02E_R_A04xWTxFx04xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F201)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A04xWTxFx04xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A05xWTxMx01",
"Sample ID":"Norm. Area: 088_0003_02E_R_A05xWTxMx01xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F202)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A05xWTxMx01xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A06xWTxMx02",
"Sample ID":"Norm. Area: 088_0003_02E_R_A06xWTxMx02xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F203)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A06xWTxMx02xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A07xWTxMx03",
"Sample ID":"Norm. Area: 088_0003_02E_R_A07xWTxMx03xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F204)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A07xWTxMx03xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A08xWTxMx04",
"Sample ID":"Norm. Area: 088_0003_02E_R_A08xWTxMx04xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.raw (F205)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02E_R_A08xWTxMx04xconcRPneg_28SEP21_CEL_MOU_LUM_0000_04AC_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A01xWTxFx01",
"Sample ID":"Norm. Area: 088_0003_02G_R_A01xWTxFx01conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F137)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A01xWTxFx01conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A02xWTxFx02",
"Sample ID":"Norm. Area: 088_0003_02G_R_A02xWTxFx02conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F138)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A02xWTxFx02conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A03xWTxFx03",
"Sample ID":"Norm. Area: 088_0003_02G_R_A03xWTxFx03conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F139)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A03xWTxFx03conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A04xWTxFx04",
"Sample ID":"Norm. Area: 088_0003_02G_R_A04xWTxFx04conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F140)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A04xWTxFx04conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A05xWTxMx01",
"Sample ID":"Norm. Area: 088_0003_02G_R_A05xWTxMx01conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F141)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A05xWTxMx01conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A06xWTxMx02",
"Sample ID":"Norm. Area: 088_0003_02G_R_A06xWTxMx02conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F142)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A06xWTxMx02conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A07xWTxMx03",
"Sample ID":"Norm. Area: 088_0003_02G_R_A07xWTxMx03conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F143)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A07xWTxMx03conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
},
{
"Subject ID":"A08xWTxMx04",
"Sample ID":"Norm. Area: 088_0003_02G_R_A08xWTxMx04conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F144)",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"088_0003_02G_R_A08xWTxMx04conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Lin-c-Kit+ cells, a fraction where LSKs reside, were selected four weeks after tet-off and BCR-ABL induction from the BM of miR-142−/−BCR-ABL and miR-142+/+BCR-ABL mice. Lin- cells were firstly selected using Lineage depletion microbeads and c-kit+ cells were then selected using anti-mouse CD117 microbeads (both from Miltenyi Biotec, San Diego, CA).","SAMPLE_TYPE":"Cultured cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"NA"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Metabolites extraction was performed by adding chilled methanol: acetonitrile: water (2:1:1, v/v/v)) to the cell pellet of pooled Lin-c-kit+ cells followed by three freeze-thaw cycles. The lysed cells were precipitated by centrifugation at 15,000 rpm for 10 min at 4 °C and metabolites in the supernatant were vacuum concentrated and subjected to LC-MS analysis."},

"CHROMATOGRAPHY":{"INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000 RS","COLUMN_NAME":"Thermo Hypersil GOLD C18 (150 x 2.1mm, 1.9um)","COLUMN_TEMPERATURE":"40","FLOW_GRADIENT":"10 min linear gradient from 100% solvent A and 0% solvent B to 2% solvent A and 98% solvent B, and 5 min of equilibration time","FLOW_RATE":"0.35 mL/min","SOLVENT_A":"100% water; 0.1% formic acid","SOLVENT_B":"100% acetonitrile; 0.1% formic acid","CHROMATOGRAPHY_TYPE":"Reversed phase"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Fusion Tribrid Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","MS_COMMENTS":"MS1 data were acquired over a mass range of 70 to 1500 m/z in the orbitrap operated at a resolution of 60,000. MS/MS data were acquired in AcquireX mode using the iterative precursor exclusion workflow, with a stepped HCD collision energy at 20, 35 and 50, at a resolution of 30,000 in the orbitrap","ION_MODE":"NEGATIVE","MS_RESULTS_FILE":"ST002446_AN003985_Results.txt UNITS:Peak Area Has RT:Yes RT units:Minutes"}

}