{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002503","ANALYSIS_ID":"AN004113","VERSION":"1","CREATED_ON":"March 9, 2023, 5:33 pm"},

"PROJECT":{"PROJECT_TITLE":"Endothelial Cell CD36 Regulates Membrane Ceramide Formation, Exosome Fatty Acid Delivery to Tissues and Circulating Fatty Acid Levels","PROJECT_TYPE":"MS quantitative analysis","PROJECT_SUMMARY":"Endothelial cell (EC) CD36 controls tissue fatty acid (FA) uptake. Here we visualized FA transfer by ECs using click chemistry. Apical FA interaction with EC CD36 induces actin reorganization, caveolin-1 tyrosine 14 (Cav-1Y14) phosphorylation, and ceramide generation in caveolae, culminating in caveolae internalization into FA/CD36/ceramide vesicles that are secreted basolaterally as small extracellular vesicles, sEVs (exosomes). In transwells, ECs expressing fluorescent CD63 (exosomal marker) transfer FAs to underlying myotubes in CD63-labeled sEVs. In vivo FA-CD63-CD36 transfer from ECs to muscle is shown using a mouse with EC-specific expression of emGFP-CD63. CD36 depletion, actin-binding latrunculin-B, Src inhibition, Cav-1Y14 mutation, and membrane neutral sphingomyelinase inhibition by GW4869 helped map the FA-sEV pathway. Injecting GW4869 to mice reduced muscle FA uptake, raised plasma FAs and lowered glucose, mimicking prominent Cd36-/- phenotypes. The FA-sEV pathway we describe contributes to crosstalk between ECs and underlying cells and links regulation of membrane ceramide to blood FAs.","INSTITUTE":"Washington University in St. Louis","DEPARTMENT":"IM-Nutitrional Science","LABORATORY":"Abumrad Lab","LAST_NAME":"Palacios","FIRST_NAME":"Hector","ADDRESS":"West Building 00201, St. Louis, Missouri, 63110, USA","EMAIL":"hectorp@wustl.edu","PHONE":"314-362-5397"},

"STUDY":{"STUDY_TITLE":"Endothelial Cell CD36 Regulates Membrane Ceramide Formation, Exosome Fatty Acid Delivery to Tissues and Circulating Fatty Acid Levels","STUDY_TYPE":"Membrane ceramide and Fatty Acid Uptake","STUDY_SUMMARY":"Endothelial cell (EC) CD36 controls tissue fatty acid (FA) uptake. Here we visualized FA transfer by ECs using click chemistry. Apical FA interaction with EC CD36 induces actin reorganization, caveolin-1 tyrosine 14 (Cav-1Y14) phosphorylation, and ceramide generation in caveolae, culminating in caveolae internalization into FA/CD36/ceramide vesicles that are secreted basolaterally as small extracellular vesicles, sEVs (exosomes). In transwells, ECs expressing fluorescent CD63 (exosomal marker) transfer FAs to underlying myotubes in CD63-labeled sEVs. In vivo FA-CD63-CD36 transfer from ECs to muscle is shown using a mouse with EC-specific expression of emGFP-CD63. CD36 depletion, actin-binding latrunculin-B, Src inhibition, Cav-1Y14 mutation, and membrane neutral sphingomyelinase inhibition by GW4869 helped map the FA-sEV pathway. Injecting GW4869 to mice reduced muscle FA uptake, raised plasma FAs and lowered glucose, mimicking prominent Cd36-/- phenotypes. The FA-sEV pathway we describe contributes to crosstalk between ECs and underlying cells and links regulation of membrane ceramide to blood FAs.","INSTITUTE":"Washington University in St. Louis","DEPARTMENT":"IM-Nutitrional Science","LABORATORY":"Abumrad Lab","LAST_NAME":"Palacios","FIRST_NAME":"Hector","ADDRESS":"West Building 00201, St. Louis, Missouri, 63110, USA","EMAIL":"hectorp@wustl.edu","PHONE":"314-362-5397"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"5",
"Sample ID":"KO1n_05",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1n_05.mzML"}
},
{
"Subject ID":"13",
"Sample ID":"KO1n_13",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1n_13.mzML"}
},
{
"Subject ID":"21",
"Sample ID":"KO1n_21",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1n_21.mzML"}
},
{
"Subject ID":"7",
"Sample ID":"KO1p_07",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1p_07.mzML"}
},
{
"Subject ID":"15",
"Sample ID":"KO1p_15",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1p_15.mzML"}
},
{
"Subject ID":"23",
"Sample ID":"KO1p_23",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1p_23.mzML"}
},
{
"Subject ID":"6",
"Sample ID":"KO3n_06",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3n_06.mzML"}
},
{
"Subject ID":"14",
"Sample ID":"KO3n_14",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3n_14.mzML"}
},
{
"Subject ID":"22",
"Sample ID":"KO3n_22",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3n_22.mzML"}
},
{
"Subject ID":"8",
"Sample ID":"KO3p_08",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3p_08.mzML"}
},
{
"Subject ID":"16",
"Sample ID":"KO3p_16",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3p_16.mzML"}
},
{
"Subject ID":"24",
"Sample ID":"KO3p_24",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3p_24.mzML"}
},
{
"Subject ID":"1",
"Sample ID":"WT1n_01",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1n_01.mzML"}
},
{
"Subject ID":"9",
"Sample ID":"WT1n_09",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1n_09.mzML"}
},
{
"Subject ID":"17",
"Sample ID":"WT1n_17",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1n_17.mzML"}
},
{
"Subject ID":"3",
"Sample ID":"WT1p_03",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1p_03.mzML"}
},
{
"Subject ID":"11",
"Sample ID":"WT1p_11",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1p_11.mzML"}
},
{
"Subject ID":"19",
"Sample ID":"WT1p_19",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1p_19.mzML"}
},
{
"Subject ID":"2",
"Sample ID":"WT3n_02",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3n_02.mzML"}
},
{
"Subject ID":"10",
"Sample ID":"WT3n_10",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3n_10.mzML"}
},
{
"Subject ID":"18",
"Sample ID":"WT3n_18",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3n_18.mzML"}
},
{
"Subject ID":"4",
"Sample ID":"WT3p_04",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3p_04.mzML"}
},
{
"Subject ID":"12",
"Sample ID":"WT3p_12",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3p_12.mzML"}
},
{
"Subject ID":"20",
"Sample ID":"WT3p_20",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3p_20.mzML"}
},
{
"Subject ID":"1",
"Sample ID":"WT1n_01_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_01.mzML"}
},
{
"Subject ID":"2",
"Sample ID":"WT3n_02_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_02.mzML"}
},
{
"Subject ID":"3",
"Sample ID":"WT1p_03_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_03.mzML"}
},
{
"Subject ID":"4",
"Sample ID":"WT3p_04_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_04.mzML"}
},
{
"Subject ID":"5",
"Sample ID":"KO1n_05_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_05.mzML"}
},
{
"Subject ID":"6",
"Sample ID":"KO3n_06_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_06.mzML"}
},
{
"Subject ID":"7",
"Sample ID":"KO1p_07_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_07.mzML"}
},
{
"Subject ID":"8",
"Sample ID":"KO3p_08_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_08.mzML"}
},
{
"Subject ID":"9",
"Sample ID":"WT1n_09_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_09.mzML"}
},
{
"Subject ID":"10",
"Sample ID":"WT3n_10_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_10.mzML"}
},
{
"Subject ID":"11",
"Sample ID":"WT1p_11_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_11.mzML"}
},
{
"Subject ID":"12",
"Sample ID":"WT3p_12_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_12.mzML"}
},
{
"Subject ID":"13",
"Sample ID":"KO1n_13_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_13.mzML"}
},
{
"Subject ID":"14",
"Sample ID":"KO3n_14_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_14.mzML"}
},
{
"Subject ID":"15",
"Sample ID":"KO1p_15_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_15.mzML"}
},
{
"Subject ID":"16",
"Sample ID":"KO3p_16_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_16.mzML"}
},
{
"Subject ID":"17",
"Sample ID":"WT1n_17_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_17.mzML"}
},
{
"Subject ID":"18",
"Sample ID":"WT3n_18_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_18.mzML"}
},
{
"Subject ID":"19",
"Sample ID":"WT1p_19_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_19.mzML"}
},
{
"Subject ID":"20",
"Sample ID":"WT3p_20_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_20.mzML"}
},
{
"Subject ID":"21",
"Sample ID":"KO1n_21_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_21.mzML"}
},
{
"Subject ID":"22",
"Sample ID":"KO3n_22_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_22.mzML"}
},
{
"Subject ID":"23",
"Sample ID":"KO1p_23_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_23.mzML"}
},
{
"Subject ID":"24",
"Sample ID":"KO3p_24_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_24.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Samples were collected and aliquots representing the same EV counts were dried under nitrogen, and resuspended in 2:2:1 acetonitrile:methanol:H2O (v/v) (for polar metabolites, eg. fatty acids, phospholipids) or in 100% isopropanol (for less-polar metabolites, eg. ceramides). The samples were mixed on an orbital shaker (360 rpm) for 1 min at room temperature before the LC/MS analysis.","SAMPLE_TYPE":"Cultured cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"Myotube Treatment with hMEC Generated FA-sEV: hMECs grown to confluence were serum-starved and treated with OA:BSA (100µM:50µM) or BSA (50µM controls). The sEVs were isolated from media collected over 48h and particle number and protein content determined."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were collected and aliquots representing the same EV counts were dried under nitrogen, and resuspended in 2:2:1 acetonitrile:methanol:H2O (v/v) (for polar metabolites, eg. fatty acids, phospholipids) or in 100% isopropanol (for less-polar metabolites, eg. ceramides). The samples were mixed on an orbital shaker (360 rpm) for 1 min at room temperature before the LC/MS analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Waters CORTECS HILIC (100 x 2.1mm,1.6um)","SOLVENT_A":"A: 95% water, 5% acetonitrile, 20 mM ammonium bicarbonate, 0.1% ammonium hydroxide solution (25% ammonia in water), 2.5 μM medronic acid","SOLVENT_B":"B: 95% acetonitrile, 5% water, 2.5 μM medronic acid","FLOW_GRADIENT":"0 to 1 min, 90% B; 12 min, 35% B; 12.5 to 14.5 min, 25% B; 15 min, 90% B followed by a re-equilibration phase of 4","FLOW_RATE":".25ml/min","COLUMN_TEMPERATURE":"45","METHODS_FILENAME":"LC_Method.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"MS_Method.pdf"},

"MS":{"INSTRUMENT_NAME":"Thermo Orbitrap ID-X tribrid","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"-"},

"MS_METABOLITE_DATA":{
"Units":"Peak Area",

"Data":[{"Metabolite":"8:0 Octanoic acid","WT1n_01_FA":"6024318","WT3n_02_FA":"5868495","WT1p_03_FA":"4505258","WT3p_04_FA":"4852857","KO1n_05_FA":"4712712","KO3n_06_FA":"5662254","KO1p_07_FA":"5375819","KO3p_08_FA":"5427124","WT1n_09_FA":"11052060","WT3n_10_FA":"11350732","WT1p_11_FA":"10430580","WT3p_12_FA":"12529638","KO1n_13_FA":"13856810","KO3n_14_FA":"11479179","KO1p_15_FA":"11164885","KO3p_16_FA":"11377403","WT1n_17_FA":"7865014","WT3n_18_FA":"8483268","WT1p_19_FA":"7694214","WT3p_20_FA":"7564237","KO1n_21_FA":"6016050","KO3n_22_FA":"7033731","KO1p_23_FA":"6060782","KO3p_24_FA":"5102615"},{"Metabolite":"14:0 Myristic acid","WT1n_01_FA":"1185878","WT3n_02_FA":"1032863","WT1p_03_FA":"1780775","WT3p_04_FA":"2435800","KO1n_05_FA":"1134774","KO3n_06_FA":"1426916","KO1p_07_FA":"1572105","KO3p_08_FA":"854415","WT1n_09_FA":"2201592","WT3n_10_FA":"2306945","WT1p_11_FA":"2077278","WT3p_12_FA":"5493065","KO1n_13_FA":"7724039","KO3n_14_FA":"3693623","KO1p_15_FA":"2094948","KO3p_16_FA":"3623004","WT1n_17_FA":"1176171","WT3n_18_FA":"1192817","WT1p_19_FA":"4880511","WT3p_20_FA":"2428226","KO1n_21_FA":"2898656","KO3n_22_FA":"2486526","KO1p_23_FA":"1869929","KO3p_24_FA":"884189"},{"Metabolite":"16:0 Palmitic acid","WT1n_01_FA":"12127325","WT3n_02_FA":"10622659","WT1p_03_FA":"16030927","WT3p_04_FA":"25341362","KO1n_05_FA":"9438774","KO3n_06_FA":"13117621","KO1p_07_FA":"13019623","KO3p_08_FA":"8381950","WT1n_09_FA":"19197032","WT3n_10_FA":"17101576","WT1p_11_FA":"19305116","WT3p_12_FA":"36367420","KO1n_13_FA":"35722020","KO3n_14_FA":"20583562","KO1p_15_FA":"20127466","KO3p_16_FA":"25109028","WT1n_17_FA":"14660494","WT3n_18_FA":"13951457","WT1p_19_FA":"26796282","WT3p_20_FA":"25597766","KO1n_21_FA":"19798904","KO3n_22_FA":"59079720","KO1p_23_FA":"39484028","KO3p_24_FA":"10015493"},{"Metabolite":"16:1 Palmitoleic acid","WT1n_01_FA":"932776","WT3n_02_FA":"734987","WT1p_03_FA":"1721844","WT3p_04_FA":"3514028","KO1n_05_FA":"1117321","KO3n_06_FA":"1314972","KO1p_07_FA":"1930895","KO3p_08_FA":"782311","WT1n_09_FA":"2070416","WT3n_10_FA":"2820426","WT1p_11_FA":"1280194","WT3p_12_FA":"8712184","KO1n_13_FA":"13320464","KO3n_14_FA":"4613169","KO1p_15_FA":"2603465","KO3p_16_FA":"5797492","WT1n_17_FA":"693708","WT3n_18_FA":"998516","WT1p_19_FA":"7216673","WT3p_20_FA":"3046189","KO1n_21_FA":"4299951","KO3n_22_FA":"2098857","KO1p_23_FA":"1994622","KO3p_24_FA":"699531"},{"Metabolite":"18:0 Stearic acid","WT1n_01_FA":"5975823","WT3n_02_FA":"5647972","WT1p_03_FA":"7587838","WT3p_04_FA":"15084091","KO1n_05_FA":"4217518","KO3n_06_FA":"6089621","KO1p_07_FA":"6105756","KO3p_08_FA":"4015148","WT1n_09_FA":"9336024","WT3n_10_FA":"7251828","WT1p_11_FA":"8494870","WT3p_12_FA":"16821188","KO1n_13_FA":"12130309","KO3n_14_FA":"7677414","KO1p_15_FA":"10896469","KO3p_16_FA":"11169005","WT1n_17_FA":"8525398","WT3n_18_FA":"6588690","WT1p_19_FA":"11965555","WT3p_20_FA":"14130922","KO1n_21_FA":"7723477","KO3n_22_FA":"42842516","KO1p_23_FA":"33570108","KO3p_24_FA":"4680781"},{"Metabolite":"18:1 Oleic acid","WT1n_01_FA":"1288299","WT3n_02_FA":"929850","WT1p_03_FA":"2536738","WT3p_04_FA":"5133695","KO1n_05_FA":"1130065","KO3n_06_FA":"1335842","KO1p_07_FA":"1805759","KO3p_08_FA":"886343","WT1n_09_FA":"2875987","WT3n_10_FA":"2752627","WT1p_11_FA":"4115300","WT3p_12_FA":"9288196","KO1n_13_FA":"10812097","KO3n_14_FA":"4397867","KO1p_15_FA":"5059311","KO3p_16_FA":"8966793","WT1n_17_FA":"1257356","WT3n_18_FA":"1680638","WT1p_19_FA":"10383278","WT3p_20_FA":"5176635","KO1n_21_FA":"3423053","KO3n_22_FA":"4402436","KO1p_23_FA":"5260044","KO3p_24_FA":"3357678"},{"Metabolite":"18:2 Linoleic acid","WT1n_01_FA":"163272","WT3n_02_FA":"131674","WT1p_03_FA":"307290","WT3p_04_FA":"823961","KO1n_05_FA":"169590","KO3n_06_FA":"215744","KO1p_07_FA":"307718","KO3p_08_FA":"144459","WT1n_09_FA":"381450","WT3n_10_FA":"396555","WT1p_11_FA":"277073","WT3p_12_FA":"1418888","KO1n_13_FA":"2361871","KO3n_14_FA":"704038","KO1p_15_FA":"480006","KO3p_16_FA":"793115","WT1n_17_FA":"154802","WT3n_18_FA":"324079","WT1p_19_FA":"1118199","WT3p_20_FA":"523763","KO1n_21_FA":"657481","KO3n_22_FA":"547389","KO1p_23_FA":"453695","KO3p_24_FA":"221166"},{"Metabolite":"20:4 Arachidonic acid","WT1n_01_FA":"172214","WT3n_02_FA":"91438","WT1p_03_FA":"192771","WT3p_04_FA":"156544","KO1n_05_FA":"161808","KO3n_06_FA":"56434","KO1p_07_FA":"89148","KO3p_08_FA":"81793","WT1n_09_FA":"189254","WT3n_10_FA":"134982","WT1p_11_FA":"120572","WT3p_12_FA":"154415","KO1n_13_FA":"934768","KO3n_14_FA":"154282","KO1p_15_FA":"329201","KO3p_16_FA":"223993","WT1n_17_FA":"132348","WT3n_18_FA":"141228","WT1p_19_FA":"132988","WT3p_20_FA":"56690","KO1n_21_FA":"99388","KO3n_22_FA":"161855","KO1p_23_FA":"66536","KO3p_24_FA":"113722"},{"Metabolite":"22:6 Docosahexaenoic 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"Metabolites":[{"metabolite_name":"8:0 Octanoic acid"},{"metabolite_name":"14:0 Myristic acid"},{"metabolite_name":"16:0 Palmitic acid"},{"metabolite_name":"16:1 Palmitoleic acid"},{"metabolite_name":"18:0 Stearic acid"},{"metabolite_name":"18:1 Oleic acid"},{"metabolite_name":"18:2 Linoleic acid"},{"metabolite_name":"20:4 Arachidonic acid"},{"metabolite_name":"22:6 Docosahexaenoic acid"}]
}

}