{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002503","ANALYSIS_ID":"AN004114","VERSION":"1","CREATED_ON":"March 9, 2023, 5:33 pm"}, "PROJECT":{"PROJECT_TITLE":"Endothelial Cell CD36 Regulates Membrane Ceramide Formation, Exosome Fatty Acid Delivery to Tissues and Circulating Fatty Acid Levels","PROJECT_TYPE":"MS quantitative analysis","PROJECT_SUMMARY":"Endothelial cell (EC) CD36 controls tissue fatty acid (FA) uptake. Here we visualized FA transfer by ECs using click chemistry. Apical FA interaction with EC CD36 induces actin reorganization, caveolin-1 tyrosine 14 (Cav-1Y14) phosphorylation, and ceramide generation in caveolae, culminating in caveolae internalization into FA/CD36/ceramide vesicles that are secreted basolaterally as small extracellular vesicles, sEVs (exosomes). In transwells, ECs expressing fluorescent CD63 (exosomal marker) transfer FAs to underlying myotubes in CD63-labeled sEVs. In vivo FA-CD63-CD36 transfer from ECs to muscle is shown using a mouse with EC-specific expression of emGFP-CD63. CD36 depletion, actin-binding latrunculin-B, Src inhibition, Cav-1Y14 mutation, and membrane neutral sphingomyelinase inhibition by GW4869 helped map the FA-sEV pathway. Injecting GW4869 to mice reduced muscle FA uptake, raised plasma FAs and lowered glucose, mimicking prominent Cd36-/- phenotypes. The FA-sEV pathway we describe contributes to crosstalk between ECs and underlying cells and links regulation of membrane ceramide to blood FAs.","INSTITUTE":"Washington University in St. Louis","DEPARTMENT":"IM-Nutitrional Science","LABORATORY":"Abumrad Lab","LAST_NAME":"Palacios","FIRST_NAME":"Hector","ADDRESS":"West Building 00201, St. Louis, Missouri, 63110, USA","EMAIL":"hectorp@wustl.edu","PHONE":"314-362-5397"}, "STUDY":{"STUDY_TITLE":"Endothelial Cell CD36 Regulates Membrane Ceramide Formation, Exosome Fatty Acid Delivery to Tissues and Circulating Fatty Acid Levels","STUDY_TYPE":"Membrane ceramide and Fatty Acid Uptake","STUDY_SUMMARY":"Endothelial cell (EC) CD36 controls tissue fatty acid (FA) uptake. Here we visualized FA transfer by ECs using click chemistry. Apical FA interaction with EC CD36 induces actin reorganization, caveolin-1 tyrosine 14 (Cav-1Y14) phosphorylation, and ceramide generation in caveolae, culminating in caveolae internalization into FA/CD36/ceramide vesicles that are secreted basolaterally as small extracellular vesicles, sEVs (exosomes). In transwells, ECs expressing fluorescent CD63 (exosomal marker) transfer FAs to underlying myotubes in CD63-labeled sEVs. In vivo FA-CD63-CD36 transfer from ECs to muscle is shown using a mouse with EC-specific expression of emGFP-CD63. CD36 depletion, actin-binding latrunculin-B, Src inhibition, Cav-1Y14 mutation, and membrane neutral sphingomyelinase inhibition by GW4869 helped map the FA-sEV pathway. Injecting GW4869 to mice reduced muscle FA uptake, raised plasma FAs and lowered glucose, mimicking prominent Cd36-/- phenotypes. The FA-sEV pathway we describe contributes to crosstalk between ECs and underlying cells and links regulation of membrane ceramide to blood FAs.","INSTITUTE":"Washington University in St. Louis","DEPARTMENT":"IM-Nutitrional Science","LABORATORY":"Abumrad Lab","LAST_NAME":"Palacios","FIRST_NAME":"Hector","ADDRESS":"West Building 00201, St. Louis, Missouri, 63110, USA","EMAIL":"hectorp@wustl.edu","PHONE":"314-362-5397"}, "SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"5", "Sample ID":"KO1n_05", "Factors":{"Genotype":"Knock-out","Treatment":"Neg"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1n_05.mzML"} }, { "Subject ID":"13", "Sample ID":"KO1n_13", "Factors":{"Genotype":"Knock-out","Treatment":"Neg"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1n_13.mzML"} }, { "Subject ID":"21", "Sample ID":"KO1n_21", "Factors":{"Genotype":"Knock-out","Treatment":"Neg"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1n_21.mzML"} }, { "Subject ID":"7", "Sample ID":"KO1p_07", "Factors":{"Genotype":"Knock-out","Treatment":"Pos"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1p_07.mzML"} }, { "Subject ID":"15", "Sample ID":"KO1p_15", "Factors":{"Genotype":"Knock-out","Treatment":"Pos"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1p_15.mzML"} }, { "Subject ID":"23", "Sample ID":"KO1p_23", "Factors":{"Genotype":"Knock-out","Treatment":"Pos"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1p_23.mzML"} }, { "Subject ID":"6", "Sample ID":"KO3n_06", "Factors":{"Genotype":"Knock-out","Treatment":"Neg"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3n_06.mzML"} }, { "Subject ID":"14", "Sample ID":"KO3n_14", "Factors":{"Genotype":"Knock-out","Treatment":"Neg"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3n_14.mzML"} }, { "Subject ID":"22", "Sample ID":"KO3n_22", "Factors":{"Genotype":"Knock-out","Treatment":"Neg"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3n_22.mzML"} }, { "Subject ID":"8", "Sample ID":"KO3p_08", "Factors":{"Genotype":"Knock-out","Treatment":"Pos"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3p_08.mzML"} }, { "Subject ID":"16", "Sample ID":"KO3p_16", "Factors":{"Genotype":"Knock-out","Treatment":"Pos"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3p_16.mzML"} }, { "Subject ID":"24", "Sample ID":"KO3p_24", "Factors":{"Genotype":"Knock-out","Treatment":"Pos"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3p_24.mzML"} }, { "Subject ID":"1", "Sample ID":"WT1n_01", "Factors":{"Genotype":"Wild-type","Treatment":"Neg"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1n_01.mzML"} }, { "Subject ID":"9", "Sample ID":"WT1n_09", "Factors":{"Genotype":"Wild-type","Treatment":"Neg"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1n_09.mzML"} }, { "Subject ID":"17", "Sample ID":"WT1n_17", "Factors":{"Genotype":"Wild-type","Treatment":"Neg"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1n_17.mzML"} }, { "Subject ID":"3", "Sample ID":"WT1p_03", "Factors":{"Genotype":"Wild-type","Treatment":"Pos"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1p_03.mzML"} }, { "Subject ID":"11", "Sample ID":"WT1p_11", "Factors":{"Genotype":"Wild-type","Treatment":"Pos"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1p_11.mzML"} }, { "Subject ID":"19", "Sample ID":"WT1p_19", "Factors":{"Genotype":"Wild-type","Treatment":"Pos"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1p_19.mzML"} }, { "Subject ID":"2", "Sample ID":"WT3n_02", "Factors":{"Genotype":"Wild-type","Treatment":"Neg"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3n_02.mzML"} }, { "Subject ID":"10", "Sample ID":"WT3n_10", "Factors":{"Genotype":"Wild-type","Treatment":"Neg"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3n_10.mzML"} }, { "Subject ID":"18", "Sample ID":"WT3n_18", "Factors":{"Genotype":"Wild-type","Treatment":"Neg"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3n_18.mzML"} }, { "Subject ID":"4", "Sample ID":"WT3p_04", "Factors":{"Genotype":"Wild-type","Treatment":"Pos"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3p_04.mzML"} }, { "Subject ID":"12", "Sample ID":"WT3p_12", "Factors":{"Genotype":"Wild-type","Treatment":"Pos"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3p_12.mzML"} }, { "Subject ID":"20", "Sample ID":"WT3p_20", "Factors":{"Genotype":"Wild-type","Treatment":"Pos"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3p_20.mzML"} }, { "Subject ID":"1", "Sample ID":"WT1n_01_FA", "Factors":{"Genotype":"Wild-type","Treatment":"Neg"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_01.mzML"} }, { "Subject ID":"2", "Sample ID":"WT3n_02_FA", "Factors":{"Genotype":"Wild-type","Treatment":"Neg"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_02.mzML"} }, { "Subject ID":"3", "Sample ID":"WT1p_03_FA", "Factors":{"Genotype":"Wild-type","Treatment":"Pos"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_03.mzML"} }, { "Subject ID":"4", "Sample ID":"WT3p_04_FA", "Factors":{"Genotype":"Wild-type","Treatment":"Pos"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_04.mzML"} }, { "Subject ID":"5", "Sample ID":"KO1n_05_FA", "Factors":{"Genotype":"Knock-out","Treatment":"Neg"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_05.mzML"} }, { "Subject ID":"6", "Sample ID":"KO3n_06_FA", "Factors":{"Genotype":"Knock-out","Treatment":"Neg"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_06.mzML"} }, { "Subject ID":"7", "Sample ID":"KO1p_07_FA", "Factors":{"Genotype":"Knock-out","Treatment":"Pos"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_07.mzML"} }, { "Subject ID":"8", "Sample ID":"KO3p_08_FA", "Factors":{"Genotype":"Knock-out","Treatment":"Pos"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_08.mzML"} }, { "Subject ID":"9", "Sample ID":"WT1n_09_FA", "Factors":{"Genotype":"Wild-type","Treatment":"Neg"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_09.mzML"} }, { "Subject ID":"10", "Sample ID":"WT3n_10_FA", "Factors":{"Genotype":"Wild-type","Treatment":"Neg"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_10.mzML"} }, { "Subject ID":"11", "Sample ID":"WT1p_11_FA", "Factors":{"Genotype":"Wild-type","Treatment":"Pos"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_11.mzML"} }, { "Subject ID":"12", "Sample ID":"WT3p_12_FA", "Factors":{"Genotype":"Wild-type","Treatment":"Pos"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_12.mzML"} }, { "Subject ID":"13", "Sample ID":"KO1n_13_FA", "Factors":{"Genotype":"Knock-out","Treatment":"Neg"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_13.mzML"} }, { "Subject ID":"14", "Sample ID":"KO3n_14_FA", "Factors":{"Genotype":"Knock-out","Treatment":"Neg"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_14.mzML"} }, { "Subject ID":"15", "Sample ID":"KO1p_15_FA", "Factors":{"Genotype":"Knock-out","Treatment":"Pos"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_15.mzML"} }, { "Subject ID":"16", "Sample ID":"KO3p_16_FA", "Factors":{"Genotype":"Knock-out","Treatment":"Pos"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_16.mzML"} }, { "Subject ID":"17", "Sample ID":"WT1n_17_FA", "Factors":{"Genotype":"Wild-type","Treatment":"Neg"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_17.mzML"} }, { "Subject ID":"18", "Sample ID":"WT3n_18_FA", "Factors":{"Genotype":"Wild-type","Treatment":"Neg"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_18.mzML"} }, { "Subject ID":"19", "Sample ID":"WT1p_19_FA", "Factors":{"Genotype":"Wild-type","Treatment":"Pos"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_19.mzML"} }, { "Subject ID":"20", "Sample ID":"WT3p_20_FA", "Factors":{"Genotype":"Wild-type","Treatment":"Pos"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_20.mzML"} }, { "Subject ID":"21", "Sample ID":"KO1n_21_FA", "Factors":{"Genotype":"Knock-out","Treatment":"Neg"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_21.mzML"} }, { "Subject ID":"22", "Sample ID":"KO3n_22_FA", "Factors":{"Genotype":"Knock-out","Treatment":"Neg"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_22.mzML"} }, { "Subject ID":"23", "Sample ID":"KO1p_23_FA", "Factors":{"Genotype":"Knock-out","Treatment":"Pos"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_23.mzML"} }, { "Subject ID":"24", "Sample ID":"KO3p_24_FA", "Factors":{"Genotype":"Knock-out","Treatment":"Pos"}, "Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_24.mzML"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Samples were collected and aliquots representing the same EV counts were dried under nitrogen, and resuspended in 2:2:1 acetonitrile:methanol:H2O (v/v) (for polar metabolites, eg. fatty acids, phospholipids) or in 100% isopropanol (for less-polar metabolites, eg. ceramides). The samples were mixed on an orbital shaker (360 rpm) for 1 min at room temperature before the LC/MS analysis.","SAMPLE_TYPE":"Cultured cells"}, "TREATMENT":{"TREATMENT_SUMMARY":"Myotube Treatment with hMEC Generated FA-sEV: hMECs grown to confluence were serum-starved and treated with OA:BSA (100µM:50µM) or BSA (50µM controls). The sEVs were isolated from media collected over 48h and particle number and protein content determined."}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were collected and aliquots representing the same EV counts were dried under nitrogen, and resuspended in 2:2:1 acetonitrile:methanol:H2O (v/v) (for polar metabolites, eg. fatty acids, phospholipids) or in 100% isopropanol (for less-polar metabolites, eg. ceramides). The samples were mixed on an orbital shaker (360 rpm) for 1 min at room temperature before the LC/MS analysis."}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Waters CORTECS HILIC (100 x 2.1mm,1.6um)","SOLVENT_A":"A: 95% water, 5% acetonitrile, 20 mM ammonium bicarbonate, 0.1% ammonium hydroxide solution (25% ammonia in water), 2.5 μM medronic acid","SOLVENT_B":"B: 95% acetonitrile, 5% water, 2.5 μM medronic acid","FLOW_GRADIENT":"0 to 1 min, 90% B; 12 min, 35% B; 12.5 to 14.5 min, 25% B; 15 min, 90% B followed by a re-equilibration phase of 4","FLOW_RATE":".25ml/min","COLUMN_TEMPERATURE":"45","METHODS_FILENAME":"LC_Method.pdf"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"MS_Method.pdf"}, "MS":{"INSTRUMENT_NAME":"Thermo Orbitrap ID-X tribrid","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"-"}, "MS_METABOLITE_DATA":{ "Units":"Peak Area", "Data":[{"Metabolite":"14:0 Myristic acid","WT1n_01_FA":"5174412","WT3n_02_FA":"4499299","WT1p_03_FA":"7213572","WT3p_04_FA":"9330013","KO1n_05_FA":"4240502","KO3n_06_FA":"5783439","KO1p_07_FA":"6230174","KO3p_08_FA":"3742426","WT1n_09_FA":"7848177","WT3n_10_FA":"9324360","WT1p_11_FA":"7405458","WT3p_12_FA":"19819936","KO1n_13_FA":"27167100","KO3n_14_FA":"13764636","KO1p_15_FA":"7988237","KO3p_16_FA":"12877522","WT1n_17_FA":"5147082","WT3n_18_FA":"4207413","WT1p_19_FA":"17974282","WT3p_20_FA":"8986743","KO1n_21_FA":"10706074","KO3n_22_FA":"9938686","KO1p_23_FA":"7236720","KO3p_24_FA":"3097104"},{"Metabolite":"16:0 Palmitic acid","WT1n_01_FA":"39366300","WT3n_02_FA":"34815072","WT1p_03_FA":"55607364","WT3p_04_FA":"88705360","KO1n_05_FA":"31658364","KO3n_06_FA":"41503328","KO1p_07_FA":"41878436","KO3p_08_FA":"26317482","WT1n_09_FA":"65774520","WT3n_10_FA":"58825428","WT1p_11_FA":"66410664","WT3p_12_FA":"118884016","KO1n_13_FA":"113863360","KO3n_14_FA":"70514784","KO1p_15_FA":"65597904","KO3p_16_FA":"81915792","WT1n_17_FA":"48110024","WT3n_18_FA":"46474204","WT1p_19_FA":"93923640","WT3p_20_FA":"83072136","KO1n_21_FA":"65935236","KO3n_22_FA":"193837904","KO1p_23_FA":"136372752","KO3p_24_FA":"33984828"},{"Metabolite":"16:1 Palmitoleic acid","WT1n_01_FA":"3654700","WT3n_02_FA":"3183822","WT1p_03_FA":"6753332","WT3p_04_FA":"12696920","KO1n_05_FA":"4591224","KO3n_06_FA":"4510049","KO1p_07_FA":"6350590","KO3p_08_FA":"2904515","WT1n_09_FA":"8173620","WT3n_10_FA":"9795025","WT1p_11_FA":"5040198","WT3p_12_FA":"29136504","KO1n_13_FA":"46582192","KO3n_14_FA":"16965616","KO1p_15_FA":"9332579","KO3p_16_FA":"20452512","WT1n_17_FA":"3110572","WT3n_18_FA":"4162394","WT1p_19_FA":"27234002","WT3p_20_FA":"10992311","KO1n_21_FA":"15644136","KO3n_22_FA":"8259623","KO1p_23_FA":"7312306","KO3p_24_FA":"2613202"},{"Metabolite":"18:0 Stearic acid","WT1n_01_FA":"34629372","WT3n_02_FA":"32159584","WT1p_03_FA":"32261404","WT3p_04_FA":"69251072","KO1n_05_FA":"29829112","KO3n_06_FA":"34720772","KO1p_07_FA":"34972152","KO3p_08_FA":"27852610","WT1n_09_FA":"50106988","WT3n_10_FA":"42594608","WT1p_11_FA":"43390988","WT3p_12_FA":"77338200","KO1n_13_FA":"54581448","KO3n_14_FA":"44347744","KO1p_15_FA":"51928552","KO3p_16_FA":"53519028","WT1n_17_FA":"46769004","WT3n_18_FA":"40467368","WT1p_19_FA":"59319432","WT3p_20_FA":"75947976","KO1n_21_FA":"42862348","KO3n_22_FA":"189841232","KO1p_23_FA":"133486416","KO3p_24_FA":"22137404"},{"Metabolite":"18:1 Oleic acid","WT1n_01_FA":"6380513","WT3n_02_FA":"4843296","WT1p_03_FA":"9724976","WT3p_04_FA":"19117218","KO1n_05_FA":"5631393","KO3n_06_FA":"6096055","KO1p_07_FA":"7850957","KO3p_08_FA":"4844458","WT1n_09_FA":"12460580","WT3n_10_FA":"9869190","WT1p_11_FA":"16991828","WT3p_12_FA":"32322382","KO1n_13_FA":"39550304","KO3n_14_FA":"17704512","KO1p_15_FA":"17251704","KO3p_16_FA":"33463742","WT1n_17_FA":"6282863","WT3n_18_FA":"8086649","WT1p_19_FA":"38695932","WT3p_20_FA":"21284118","KO1n_21_FA":"14813989","KO3n_22_FA":"18685788","KO1p_23_FA":"19683022","KO3p_24_FA":"12338788"},{"Metabolite":"18:2 Linoleic acid","WT1n_01_FA":"997400","WT3n_02_FA":"703669","WT1p_03_FA":"1449890","WT3p_04_FA":"3038347","KO1n_05_FA":"967308","KO3n_06_FA":"957268","KO1p_07_FA":"1314698","KO3p_08_FA":"761571","WT1n_09_FA":"1512459","WT3n_10_FA":"1852912","WT1p_11_FA":"1173704","WT3p_12_FA":"5165102","KO1n_13_FA":"8380669","KO3n_14_FA":"2959558","KO1p_15_FA":"1778942","KO3p_16_FA":"3025610","WT1n_17_FA":"878234","WT3n_18_FA":"1525928","WT1p_19_FA":"4379050","WT3p_20_FA":"1997698","KO1n_21_FA":"2535115","KO3n_22_FA":"2095674","KO1p_23_FA":"1839744","KO3p_24_FA":"1204400"},{"Metabolite":"18:3 alpha-Linolenic acid","WT1n_01_FA":"223414","WT3n_02_FA":"267130","WT1p_03_FA":"289923","WT3p_04_FA":"322422","KO1n_05_FA":"162293","KO3n_06_FA":"262378","KO1p_07_FA":"250594","KO3p_08_FA":"206119","WT1n_09_FA":"249243","WT3n_10_FA":"291429","WT1p_11_FA":"281585","WT3p_12_FA":"660271","KO1n_13_FA":"736758","KO3n_14_FA":"240545","KO1p_15_FA":"540737","KO3p_16_FA":"719003","WT1n_17_FA":"268226","WT3n_18_FA":"165244","WT1p_19_FA":"544781","WT3p_20_FA":"551440","KO1n_21_FA":"198302","KO3n_22_FA":"1267389","KO1p_23_FA":"1108248","KO3p_24_FA":"238126"},{"Metabolite":"20:4 Arachidonic acid","WT1n_01_FA":"698245","WT3n_02_FA":"389426","WT1p_03_FA":"801037","WT3p_04_FA":"625398","KO1n_05_FA":"625513","KO3n_06_FA":"291024","KO1p_07_FA":"356511","KO3p_08_FA":"341782","WT1n_09_FA":"780878","WT3n_10_FA":"642567","WT1p_11_FA":"476001","WT3p_12_FA":"613929","KO1n_13_FA":"3467324","KO3n_14_FA":"686537","KO1p_15_FA":"1309384","KO3p_16_FA":"911856","WT1n_17_FA":"569848","WT3n_18_FA":"597243","WT1p_19_FA":"533066","WT3p_20_FA":"253269","KO1n_21_FA":"420401","KO3n_22_FA":"727050","KO1p_23_FA":"252215","KO3p_24_FA":"495053"},{"Metabolite":"22:6 Docosahexaenoic acid","WT1n_01_FA":"0","WT3n_02_FA":"105589","WT1p_03_FA":"134171","WT3p_04_FA":"187493","KO1n_05_FA":"144104","KO3n_06_FA":"61058","KO1p_07_FA":"120801","KO3p_08_FA":"133265","WT1n_09_FA":"248533","WT3n_10_FA":"285394","WT1p_11_FA":"216920","WT3p_12_FA":"313820","KO1n_13_FA":"1121396","KO3n_14_FA":"291238","KO1p_15_FA":"478154","KO3p_16_FA":"421420","WT1n_17_FA":"144324","WT3n_18_FA":"231157","WT1p_19_FA":"0","WT3p_20_FA":"66033","KO1n_21_FA":"133115","KO3n_22_FA":"206941","KO1p_23_FA":"0","KO3p_24_FA":"118005"}], "Metabolites":[{"metabolite_name":"14:0 Myristic acid"},{"metabolite_name":"16:0 Palmitic acid"},{"metabolite_name":"16:1 Palmitoleic acid"},{"metabolite_name":"18:0 Stearic acid"},{"metabolite_name":"18:1 Oleic acid"},{"metabolite_name":"18:2 Linoleic acid"},{"metabolite_name":"18:3 alpha-Linolenic acid"},{"metabolite_name":"20:4 Arachidonic acid"},{"metabolite_name":"22:6 Docosahexaenoic acid"}] } }