{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002503","ANALYSIS_ID":"AN004114","VERSION":"1","CREATED_ON":"March 9, 2023, 5:33 pm"},

"PROJECT":{"PROJECT_TITLE":"Endothelial Cell CD36 Regulates Membrane Ceramide Formation, Exosome Fatty Acid Delivery to Tissues and Circulating Fatty Acid Levels","PROJECT_TYPE":"MS quantitative analysis","PROJECT_SUMMARY":"Endothelial cell (EC) CD36 controls tissue fatty acid (FA) uptake. Here we visualized FA transfer by ECs using click chemistry. Apical FA interaction with EC CD36 induces actin reorganization, caveolin-1 tyrosine 14 (Cav-1Y14) phosphorylation, and ceramide generation in caveolae, culminating in caveolae internalization into FA/CD36/ceramide vesicles that are secreted basolaterally as small extracellular vesicles, sEVs (exosomes). In transwells, ECs expressing fluorescent CD63 (exosomal marker) transfer FAs to underlying myotubes in CD63-labeled sEVs. In vivo FA-CD63-CD36 transfer from ECs to muscle is shown using a mouse with EC-specific expression of emGFP-CD63. CD36 depletion, actin-binding latrunculin-B, Src inhibition, Cav-1Y14 mutation, and membrane neutral sphingomyelinase inhibition by GW4869 helped map the FA-sEV pathway. Injecting GW4869 to mice reduced muscle FA uptake, raised plasma FAs and lowered glucose, mimicking prominent Cd36-/- phenotypes. The FA-sEV pathway we describe contributes to crosstalk between ECs and underlying cells and links regulation of membrane ceramide to blood FAs.","INSTITUTE":"Washington University in St. Louis","DEPARTMENT":"IM-Nutitrional Science","LABORATORY":"Abumrad Lab","LAST_NAME":"Palacios","FIRST_NAME":"Hector","ADDRESS":"West Building 00201, St. Louis, Missouri, 63110, USA","EMAIL":"hectorp@wustl.edu","PHONE":"314-362-5397"},

"STUDY":{"STUDY_TITLE":"Endothelial Cell CD36 Regulates Membrane Ceramide Formation, Exosome Fatty Acid Delivery to Tissues and Circulating Fatty Acid Levels","STUDY_TYPE":"Membrane ceramide and Fatty Acid Uptake","STUDY_SUMMARY":"Endothelial cell (EC) CD36 controls tissue fatty acid (FA) uptake. Here we visualized FA transfer by ECs using click chemistry. Apical FA interaction with EC CD36 induces actin reorganization, caveolin-1 tyrosine 14 (Cav-1Y14) phosphorylation, and ceramide generation in caveolae, culminating in caveolae internalization into FA/CD36/ceramide vesicles that are secreted basolaterally as small extracellular vesicles, sEVs (exosomes). In transwells, ECs expressing fluorescent CD63 (exosomal marker) transfer FAs to underlying myotubes in CD63-labeled sEVs. In vivo FA-CD63-CD36 transfer from ECs to muscle is shown using a mouse with EC-specific expression of emGFP-CD63. CD36 depletion, actin-binding latrunculin-B, Src inhibition, Cav-1Y14 mutation, and membrane neutral sphingomyelinase inhibition by GW4869 helped map the FA-sEV pathway. Injecting GW4869 to mice reduced muscle FA uptake, raised plasma FAs and lowered glucose, mimicking prominent Cd36-/- phenotypes. The FA-sEV pathway we describe contributes to crosstalk between ECs and underlying cells and links regulation of membrane ceramide to blood FAs.","INSTITUTE":"Washington University in St. Louis","DEPARTMENT":"IM-Nutitrional Science","LABORATORY":"Abumrad Lab","LAST_NAME":"Palacios","FIRST_NAME":"Hector","ADDRESS":"West Building 00201, St. Louis, Missouri, 63110, USA","EMAIL":"hectorp@wustl.edu","PHONE":"314-362-5397"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"5",
"Sample ID":"KO1n_05",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1n_05.mzML"}
},
{
"Subject ID":"13",
"Sample ID":"KO1n_13",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1n_13.mzML"}
},
{
"Subject ID":"21",
"Sample ID":"KO1n_21",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1n_21.mzML"}
},
{
"Subject ID":"7",
"Sample ID":"KO1p_07",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1p_07.mzML"}
},
{
"Subject ID":"15",
"Sample ID":"KO1p_15",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1p_15.mzML"}
},
{
"Subject ID":"23",
"Sample ID":"KO1p_23",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO1p_23.mzML"}
},
{
"Subject ID":"6",
"Sample ID":"KO3n_06",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3n_06.mzML"}
},
{
"Subject ID":"14",
"Sample ID":"KO3n_14",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3n_14.mzML"}
},
{
"Subject ID":"22",
"Sample ID":"KO3n_22",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3n_22.mzML"}
},
{
"Subject ID":"8",
"Sample ID":"KO3p_08",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3p_08.mzML"}
},
{
"Subject ID":"16",
"Sample ID":"KO3p_16",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3p_16.mzML"}
},
{
"Subject ID":"24",
"Sample ID":"KO3p_24",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_KO3p_24.mzML"}
},
{
"Subject ID":"1",
"Sample ID":"WT1n_01",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1n_01.mzML"}
},
{
"Subject ID":"9",
"Sample ID":"WT1n_09",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1n_09.mzML"}
},
{
"Subject ID":"17",
"Sample ID":"WT1n_17",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1n_17.mzML"}
},
{
"Subject ID":"3",
"Sample ID":"WT1p_03",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1p_03.mzML"}
},
{
"Subject ID":"11",
"Sample ID":"WT1p_11",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1p_11.mzML"}
},
{
"Subject ID":"19",
"Sample ID":"WT1p_19",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT1p_19.mzML"}
},
{
"Subject ID":"2",
"Sample ID":"WT3n_02",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3n_02.mzML"}
},
{
"Subject ID":"10",
"Sample ID":"WT3n_10",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3n_10.mzML"}
},
{
"Subject ID":"18",
"Sample ID":"WT3n_18",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3n_18.mzML"}
},
{
"Subject ID":"4",
"Sample ID":"WT3p_04",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3p_04.mzML"}
},
{
"Subject ID":"12",
"Sample ID":"WT3p_12",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3p_12.mzML"}
},
{
"Subject ID":"20",
"Sample ID":"WT3p_20",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"HSS_Nada_Exo_Pos_IPA_WT3p_20.mzML"}
},
{
"Subject ID":"1",
"Sample ID":"WT1n_01_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_01.mzML"}
},
{
"Subject ID":"2",
"Sample ID":"WT3n_02_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_02.mzML"}
},
{
"Subject ID":"3",
"Sample ID":"WT1p_03_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_03.mzML"}
},
{
"Subject ID":"4",
"Sample ID":"WT3p_04_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_04.mzML"}
},
{
"Subject ID":"5",
"Sample ID":"KO1n_05_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_05.mzML"}
},
{
"Subject ID":"6",
"Sample ID":"KO3n_06_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_06.mzML"}
},
{
"Subject ID":"7",
"Sample ID":"KO1p_07_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_07.mzML"}
},
{
"Subject ID":"8",
"Sample ID":"KO3p_08_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_08.mzML"}
},
{
"Subject ID":"9",
"Sample ID":"WT1n_09_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_09.mzML"}
},
{
"Subject ID":"10",
"Sample ID":"WT3n_10_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_10.mzML"}
},
{
"Subject ID":"11",
"Sample ID":"WT1p_11_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_11.mzML"}
},
{
"Subject ID":"12",
"Sample ID":"WT3p_12_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_12.mzML"}
},
{
"Subject ID":"13",
"Sample ID":"KO1n_13_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_13.mzML"}
},
{
"Subject ID":"14",
"Sample ID":"KO3n_14_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_14.mzML"}
},
{
"Subject ID":"15",
"Sample ID":"KO1p_15_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_15.mzML"}
},
{
"Subject ID":"16",
"Sample ID":"KO3p_16_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_16.mzML"}
},
{
"Subject ID":"17",
"Sample ID":"WT1n_17_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_17.mzML"}
},
{
"Subject ID":"18",
"Sample ID":"WT3n_18_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_18.mzML"}
},
{
"Subject ID":"19",
"Sample ID":"WT1p_19_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_19.mzML"}
},
{
"Subject ID":"20",
"Sample ID":"WT3p_20_FA",
"Factors":{"Genotype":"Wild-type","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_20.mzML"}
},
{
"Subject ID":"21",
"Sample ID":"KO1n_21_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_21.mzML"}
},
{
"Subject ID":"22",
"Sample ID":"KO3n_22_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Neg"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_22.mzML"}
},
{
"Subject ID":"23",
"Sample ID":"KO1p_23_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_23.mzML"}
},
{
"Subject ID":"24",
"Sample ID":"KO3p_24_FA",
"Factors":{"Genotype":"Knock-out","Treatment":"Pos"},
"Additional sample data":{"Batch":"1b","RAW_FILE_NAME":"Nada_iHILIC_Exosomes_221_PNS_24.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Samples were collected and aliquots representing the same EV counts were dried under nitrogen, and resuspended in 2:2:1 acetonitrile:methanol:H2O (v/v) (for polar metabolites, eg. fatty acids, phospholipids) or in 100% isopropanol (for less-polar metabolites, eg. ceramides). The samples were mixed on an orbital shaker (360 rpm) for 1 min at room temperature before the LC/MS analysis.","SAMPLE_TYPE":"Cultured cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"Myotube Treatment with hMEC Generated FA-sEV: hMECs grown to confluence were serum-starved and treated with OA:BSA (100µM:50µM) or BSA (50µM controls). The sEVs were isolated from media collected over 48h and particle number and protein content determined."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were collected and aliquots representing the same EV counts were dried under nitrogen, and resuspended in 2:2:1 acetonitrile:methanol:H2O (v/v) (for polar metabolites, eg. fatty acids, phospholipids) or in 100% isopropanol (for less-polar metabolites, eg. ceramides). The samples were mixed on an orbital shaker (360 rpm) for 1 min at room temperature before the LC/MS analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Waters CORTECS HILIC (100 x 2.1mm,1.6um)","SOLVENT_A":"A: 95% water, 5% acetonitrile, 20 mM ammonium bicarbonate, 0.1% ammonium hydroxide solution (25% ammonia in water), 2.5 μM medronic acid","SOLVENT_B":"B: 95% acetonitrile, 5% water, 2.5 μM medronic acid","FLOW_GRADIENT":"0 to 1 min, 90% B; 12 min, 35% B; 12.5 to 14.5 min, 25% B; 15 min, 90% B followed by a re-equilibration phase of 4","FLOW_RATE":".25ml/min","COLUMN_TEMPERATURE":"45","METHODS_FILENAME":"LC_Method.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"MS_Method.pdf"},

"MS":{"INSTRUMENT_NAME":"Thermo Orbitrap ID-X tribrid","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"-"},

"MS_METABOLITE_DATA":{
"Units":"Peak Area",

"Data":[{"Metabolite":"14:0 Myristic acid","WT1n_01_FA":"5174412","WT3n_02_FA":"4499299","WT1p_03_FA":"7213572","WT3p_04_FA":"9330013","KO1n_05_FA":"4240502","KO3n_06_FA":"5783439","KO1p_07_FA":"6230174","KO3p_08_FA":"3742426","WT1n_09_FA":"7848177","WT3n_10_FA":"9324360","WT1p_11_FA":"7405458","WT3p_12_FA":"19819936","KO1n_13_FA":"27167100","KO3n_14_FA":"13764636","KO1p_15_FA":"7988237","KO3p_16_FA":"12877522","WT1n_17_FA":"5147082","WT3n_18_FA":"4207413","WT1p_19_FA":"17974282","WT3p_20_FA":"8986743","KO1n_21_FA":"10706074","KO3n_22_FA":"9938686","KO1p_23_FA":"7236720","KO3p_24_FA":"3097104"},{"Metabolite":"16:0 Palmitic acid","WT1n_01_FA":"39366300","WT3n_02_FA":"34815072","WT1p_03_FA":"55607364","WT3p_04_FA":"88705360","KO1n_05_FA":"31658364","KO3n_06_FA":"41503328","KO1p_07_FA":"41878436","KO3p_08_FA":"26317482","WT1n_09_FA":"65774520","WT3n_10_FA":"58825428","WT1p_11_FA":"66410664","WT3p_12_FA":"118884016","KO1n_13_FA":"113863360","KO3n_14_FA":"70514784","KO1p_15_FA":"65597904","KO3p_16_FA":"81915792","WT1n_17_FA":"48110024","WT3n_18_FA":"46474204","WT1p_19_FA":"93923640","WT3p_20_FA":"83072136","KO1n_21_FA":"65935236","KO3n_22_FA":"193837904","KO1p_23_FA":"136372752","KO3p_24_FA":"33984828"},{"Metabolite":"16:1 Palmitoleic acid","WT1n_01_FA":"3654700","WT3n_02_FA":"3183822","WT1p_03_FA":"6753332","WT3p_04_FA":"12696920","KO1n_05_FA":"4591224","KO3n_06_FA":"4510049","KO1p_07_FA":"6350590","KO3p_08_FA":"2904515","WT1n_09_FA":"8173620","WT3n_10_FA":"9795025","WT1p_11_FA":"5040198","WT3p_12_FA":"29136504","KO1n_13_FA":"46582192","KO3n_14_FA":"16965616","KO1p_15_FA":"9332579","KO3p_16_FA":"20452512","WT1n_17_FA":"3110572","WT3n_18_FA":"4162394","WT1p_19_FA":"27234002","WT3p_20_FA":"10992311","KO1n_21_FA":"15644136","KO3n_22_FA":"8259623","KO1p_23_FA":"7312306","KO3p_24_FA":"2613202"},{"Metabolite":"18:0 Stearic acid","WT1n_01_FA":"34629372","WT3n_02_FA":"32159584","WT1p_03_FA":"32261404","WT3p_04_FA":"69251072","KO1n_05_FA":"29829112","KO3n_06_FA":"34720772","KO1p_07_FA":"34972152","KO3p_08_FA":"27852610","WT1n_09_FA":"50106988","WT3n_10_FA":"42594608","WT1p_11_FA":"43390988","WT3p_12_FA":"77338200","KO1n_13_FA":"54581448","KO3n_14_FA":"44347744","KO1p_15_FA":"51928552","KO3p_16_FA":"53519028","WT1n_17_FA":"46769004","WT3n_18_FA":"40467368","WT1p_19_FA":"59319432","WT3p_20_FA":"75947976","KO1n_21_FA":"42862348","KO3n_22_FA":"189841232","KO1p_23_FA":"133486416","KO3p_24_FA":"22137404"},{"Metabolite":"18:1 Oleic acid","WT1n_01_FA":"6380513","WT3n_02_FA":"4843296","WT1p_03_FA":"9724976","WT3p_04_FA":"19117218","KO1n_05_FA":"5631393","KO3n_06_FA":"6096055","KO1p_07_FA":"7850957","KO3p_08_FA":"4844458","WT1n_09_FA":"12460580","WT3n_10_FA":"9869190","WT1p_11_FA":"16991828","WT3p_12_FA":"32322382","KO1n_13_FA":"39550304","KO3n_14_FA":"17704512","KO1p_15_FA":"17251704","KO3p_16_FA":"33463742","WT1n_17_FA":"6282863","WT3n_18_FA":"8086649","WT1p_19_FA":"38695932","WT3p_20_FA":"21284118","KO1n_21_FA":"14813989","KO3n_22_FA":"18685788","KO1p_23_FA":"19683022","KO3p_24_FA":"12338788"},{"Metabolite":"18:2 Linoleic acid","WT1n_01_FA":"997400","WT3n_02_FA":"703669","WT1p_03_FA":"1449890","WT3p_04_FA":"3038347","KO1n_05_FA":"967308","KO3n_06_FA":"957268","KO1p_07_FA":"1314698","KO3p_08_FA":"761571","WT1n_09_FA":"1512459","WT3n_10_FA":"1852912","WT1p_11_FA":"1173704","WT3p_12_FA":"5165102","KO1n_13_FA":"8380669","KO3n_14_FA":"2959558","KO1p_15_FA":"1778942","KO3p_16_FA":"3025610","WT1n_17_FA":"878234","WT3n_18_FA":"1525928","WT1p_19_FA":"4379050","WT3p_20_FA":"1997698","KO1n_21_FA":"2535115","KO3n_22_FA":"2095674","KO1p_23_FA":"1839744","KO3p_24_FA":"1204400"},{"Metabolite":"18:3 alpha-Linolenic acid","WT1n_01_FA":"223414","WT3n_02_FA":"267130","WT1p_03_FA":"289923","WT3p_04_FA":"322422","KO1n_05_FA":"162293","KO3n_06_FA":"262378","KO1p_07_FA":"250594","KO3p_08_FA":"206119","WT1n_09_FA":"249243","WT3n_10_FA":"291429","WT1p_11_FA":"281585","WT3p_12_FA":"660271","KO1n_13_FA":"736758","KO3n_14_FA":"240545","KO1p_15_FA":"540737","KO3p_16_FA":"719003","WT1n_17_FA":"268226","WT3n_18_FA":"165244","WT1p_19_FA":"544781","WT3p_20_FA":"551440","KO1n_21_FA":"198302","KO3n_22_FA":"1267389","KO1p_23_FA":"1108248","KO3p_24_FA":"238126"},{"Metabolite":"20:4 Arachidonic acid","WT1n_01_FA":"698245","WT3n_02_FA":"389426","WT1p_03_FA":"801037","WT3p_04_FA":"625398","KO1n_05_FA":"625513","KO3n_06_FA":"291024","KO1p_07_FA":"356511","KO3p_08_FA":"341782","WT1n_09_FA":"780878","WT3n_10_FA":"642567","WT1p_11_FA":"476001","WT3p_12_FA":"613929","KO1n_13_FA":"3467324","KO3n_14_FA":"686537","KO1p_15_FA":"1309384","KO3p_16_FA":"911856","WT1n_17_FA":"569848","WT3n_18_FA":"597243","WT1p_19_FA":"533066","WT3p_20_FA":"253269","KO1n_21_FA":"420401","KO3n_22_FA":"727050","KO1p_23_FA":"252215","KO3p_24_FA":"495053"},{"Metabolite":"22:6 Docosahexaenoic 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"Metabolites":[{"metabolite_name":"14:0 Myristic acid"},{"metabolite_name":"16:0 Palmitic acid"},{"metabolite_name":"16:1 Palmitoleic acid"},{"metabolite_name":"18:0 Stearic acid"},{"metabolite_name":"18:1 Oleic acid"},{"metabolite_name":"18:2 Linoleic acid"},{"metabolite_name":"18:3 alpha-Linolenic acid"},{"metabolite_name":"20:4 Arachidonic acid"},{"metabolite_name":"22:6 Docosahexaenoic acid"}]
}

}