{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002537","ANALYSIS_ID":"AN004176","VERSION":"1","CREATED_ON":"April 3, 2023, 10:11 am"},

"PROJECT":{"PROJECT_TITLE":"Osmoprotectants play a major role in the Portulaca oleracea resistance to high levels of salinity stress - Insights from a metabolomics and proteomics integrated approach","PROJECT_SUMMARY":"Purslane is an invasive plant and is considered the eighth most common weed in the world. Because of that, its outdoor production in extensive areas faces several concerns. Kong & Zheng (2014) evaluated the potential of producing purslane in a hydroponic system, generating approximately 5.75 kg of fresh matter per m2 per month, which might yield 57.5 tons/hectare/year if cultivated in a bimestrial regime. The high productivity of purslane, when grown in controlled-environment agriculture, can open many opportunities for the purslane industry, even in the context of biosaline agriculture. Building up a robust multi-omics database on the response of purslane to salt stress and the subsequent study of it via an MOI analysis can create the basis for a future system biology approach to decode the genetics behind its resistance to salinity stress. The present study is a second step in building a robust database on the morpho-physiological and molecular responses of Portulaca oleracea L. to salinity stress and its subsequent use in attempting to decode the genetics behind its resistance to this abiotic stress. After reporting on the characterization of the morpho-physiological responses of young purslane plants to such stress using a robust salinization protocol, here we report a study on adult purslane plants through the characterization of the untargeted metabolome and proteome profiles on the leaves and roots of this halophyte species submitted to very high salinity stress, and the consequent use of single- and multi-omics analysis strategies to study it.","INSTITUTE":"Embrapa Agroenergy","LAST_NAME":"Souza Júnior","FIRST_NAME":"Manoel Teixeira","ADDRESS":"Parque Estacao Biologica final Asa Norte Brasília DF 70770-901 BR, PQEB, sn - Asa Norte, DF","EMAIL":"manoel.souza@embrapa.br","PHONE":"+55 (61) 3448-4246"},

"STUDY":{"STUDY_TITLE":"Osmoprotectants play a major role in the Portulaca oleracea resistance to high levels of salinity stress - Insights from a metabolomics and proteomics integrated approach","STUDY_SUMMARY":"Purslane is an invasive plant and is considered the eighth most common weed in the world. Because of that, its outdoor production in extensive areas faces several concerns. Kong & Zheng (2014) evaluated the potential of producing purslane in a hydroponic system, generating approximately 5.75 kg of fresh matter per m2 per month, which might yield 57.5 tons/hectare/year if cultivated in a bimestrial regime. The high productivity of purslane, when grown in controlled-environment agriculture, can open many opportunities for the purslane industry, even in the context of biosaline agriculture. Building up a robust multi-omics database on the response of purslane to salt stress and the subsequent study of it via an MOI analysis can create the basis for a future system biology approach to decode the genetics behind its resistance to salinity stress. The present study is a second step in building a robust database on the morpho-physiological and molecular responses of Portulaca oleracea L. to salinity stress and its subsequent use in attempting to decode the genetics behind its resistance to this abiotic stress. After reporting on the characterization of the morpho-physiological responses of young purslane plants to such stress using a robust salinization protocol, here we report a study on adult purslane plants through the characterization of the untargeted metabolome and proteome profiles on the leaves and roots of this halophyte species submitted to very high salinity stress, and the consequent use of single- and multi-omics analysis strategies to study it.","INSTITUTE":"Embrapa Agroenergy","LAST_NAME":"Souza Júnior","FIRST_NAME":"Manoel Teixeira","ADDRESS":"Parque Estacao Biologica final Asa Norte Brasília DF 70770-901 BR, PQEB, sn - Asa Norte, DF","EMAIL":"manoel.souza@embrapa.br","PHONE":"+55 (61) 3448-4246"},

"SUBJECT":{"SUBJECT_TYPE":"Plant","SUBJECT_SPECIES":"Portulaca oleracea","TAXONOMY_ID":"46147"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Purslane_CP1_Adult_Leaf_Polar_Positive",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP1_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP2_Adult_Leaf_Polar_Positive",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP2_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP3_Adult_Leaf_Polar_Positive",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP3_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP4_Adult_Leaf_Polar_Positive",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP4_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP1_Adult_Leaf_Polar_Negative",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP1_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP2_Adult_Leaf_Polar_Negative",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP2_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP3_Adult_Leaf_Polar_Negative",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP3_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP4_Adult_Leaf_Polar_Negative",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP4_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP1_Adult_Leaf_Lipidic_Positive",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP1_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP2_Adult_Leaf_Lipidic_Positive",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP2_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP3_Adult_Leaf_Lipidic_Positive",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP3_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP4_Adult_Leaf_Lipidic_Positive",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP4_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP1_Adult_Leaf_Lipidic_Negative",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP1_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP2_Adult_Leaf_Lipidic_Negative",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP2_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP3_Adult_Leaf_Lipidic_Negative",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP3_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP4_Adult_Leaf_Lipidic_Negative",
"Factors":{"Group":"Leaf_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP4_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP1_Adult_Leaf_Polar_Positive",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP1_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP2_Adult_Leaf_Polar_Positive",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP2_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP3_Adult_Leaf_Polar_Positive",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP3_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP4_Adult_Leaf_Polar_Positive",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP4_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP1_Adult_Leaf_Polar_Negative",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP1_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP2_Adult_Leaf_Polar_Negative",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP2_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP3_Adult_Leaf_Polar_Negative",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP3_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP4_Adult_Leaf_Polar_Negative",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP4_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP1_Adult_Leaf_Lipidic_Positive",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP1_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP2_Adult_Leaf_Lipidic_Positive",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP2_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP3_Adult_Leaf_Lipidic_Positive",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP3_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP4_Adult_Leaf_Lipidic_Positive",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP4_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP1_Adult_Leaf_Lipidic_Negative",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP1_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP2_Adult_Leaf_Lipidic_Negative",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP2_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP3_Adult_Leaf_Lipidic_Negative",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP3_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP4_Adult_Leaf_Lipidic_Negative",
"Factors":{"Group":"Leaf_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP4_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP1_Adult_Root_Lipidic_Negative",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP1_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP2_Adult_Root_Lipidic_Negative",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP2_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP3_Adult_Root_Lipidic_Negative",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP3_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP4_Adult_Root_Lipidic_Negative",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP4_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP1_Adult_Root_Lipidic_Positive",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP1_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP2_Adult_Root_Lipidic_Positive",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP2_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP3_Adult_Root_Lipidic_Positive",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP3_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP4_Adult_Root_Lipidic_Positive",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP4_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP1_Adult_Root_Polar_Negative",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP1_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP2_Adult_Root_Polar_Negative",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP2_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP3_Adult_Root_Polar_Negative",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP3_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP4_Adult_Root_Polar_Negative",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP4_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP1_Adult_Root_Polar_Positive",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP1_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP2_Adult_Root_Polar_Positive",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP2_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP3_Adult_Root_Polar_Positive",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP3_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_CP4_Adult_Root_Polar_Positive",
"Factors":{"Group":"Root_Control"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_CP4_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP1_Adult_Root_Lipidic_Negative",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP1_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP2_Adult_Root_Lipidic_Negative",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP2_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP3_Adult_Root_Lipidic_Negative",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP3_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP4_Adult_Root_Lipidic_Negative",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP4_Adult_Lipidic_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP1_Adult_Root_Lipidic_Positive",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP1_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP2_Adult_Root_Lipidic_Positive",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP2_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP3_Adult_Root_Lipidic_Positive",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP3_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP4_Adult_Root_Lipidic_Positive",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP4_Adult_Lipidic_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP1_Adult_Root_Polar_Negative",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP1_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP2_Adult_Root_Polar_Negative",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP2_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP3_Adult_Root_Polar_Negative",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP3_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP4_Adult_Root_Polar_Negative",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP4_Adult_Polar_Negative.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP1_Adult_Root_Polar_Positive",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP1_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP2_Adult_Root_Polar_Positive",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP2_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP3_Adult_Root_Polar_Positive",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP3_Adult_Polar_Positive.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Purslane_SP4_Adult_Root_Polar_Positive",
"Factors":{"Group":"Root_Stressed"},
"Additional sample data":{"RAW_FILE_NAME":"Purslane_SP4_Adult_Polar_Positive.mzXML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Leaves and roots from both treatments - five replicates per treatment - were collected for biomass and mineral analysis. Fifteen samples of substrate were collected for mineral analysis, five before salinization, and ten at the end of the experiment - five from control and five from stressed plants. Leaves and roots from both treatments - five replicates per treatment - were collected and immediately immersed in liquid nitrogen and then stored at -80 °C until extraction of metabolites or proteins.","SAMPLE_TYPE":"Plant"},

"TREATMENT":{"TREATMENT_SUMMARY":"The B1 accession of purslane (Portulaca oleracea L.) used in this study belongs to the Purslane Collection at Embrapa Agroenergia. Seeds underwent disinfection following the same procedure described in Belo Silva et al. (2022), which consisted of soaking in 2% sodium hypochlorite and Tween® 20 for five minutes, under slow agitation, and then washing with sterile water and drying on sterilized filter paper. After being seeded on a culture medium (MS 1/2 strength, Phytagel 0.2%, and pH 5.8) (Murashige and Skoog, 1962), it was kept for germination in a Growth chamber Conviron mod. Adaptis 1000TC (Controlled Environments Ltd, Winnipeg, Canada) at 150 μmol/m2/s of light and 30°C. After 13 days, seedlings were individually transferred to 200 ml plastic cups containing 100 g of sterilized substrate - clay soil, vermiculite, and a commercial substrate (Bioplant®), 2:1:1 (v:v:v) ratio – and transferred to another Conviron® growth chamber mod. PGW40 at 25±2°C, 500±20 μmol/m2/s of light, 65±5% air relative humidity, and photoperiod of 16/8 h (light/dark), and kept there until the end of the experiments. The plants were allowed to acclimatize for three days, and the salinity stress started three weeks after the end of the acclimatization period. The salinization experiment consisted of two salinity levels (0.0 and 2.0 g of NaCl / 100 g of the substrate), with 16 replicates (plants) in a completely randomized design, and the stress lasted 12 days. During the entire experiment, plants were at field capacity. To avoid the loss of Na+ or Cl-, no leakage of the saline solution was allowed to get out of the plastic cup, as described previously in Belo Silva et al. (2022). The water lost due to evapotranspiration was replaced with deionized water daily, and the electric conductivity at field capacity (ECfc) and water potential in the substrate solution was measured once - on the 8th day of stress - for all replicates."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"For the metabolomics study, the following substances were acquired from Sigma Aldrich (Merck, USA): methanol UHPLC grade, acetonitrile LC-MS grade, methyl-tert-butyl-ether, formic acid LC-MS grade, and sodium hydroxide ACS grade. Water was obtained using a Milli-Q system (Millipore, USA). Metabolites were extracted using a well-established protocol, which provides polar and lipidic fractions from the same samples. In this protocol, we first ground the plant material (roots or leaves) in a ball mill (Biospec Products, USA), then added to a microtube containing 1 mL from a solution (1:3) of methanol and methyl-tert-butyl-ether at -20°C. Samples were incubated for 10 min at 4.0 °C, then ultrasonicated for another 10 min in an ice bath. A solution (1:3) of methanol and water was added to each microtube and then submitted to centrifugation (12,000 rpm at 4.0°C for 5 min). The polar (upper) and non-polar (lower) fractions were collected and vacuum-dried in a speed vac system overnight (Centrivap, Labconco, Kansas City, MO, USA). Four microliters of the extract were resuspended in 850 μL of the methanol and water (1:3) solvent mixture and then analyzed by UHPLC-MS."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Shimadzu Nexera X2","COLUMN_NAME":"Waters ACQUITY UPLC BEH C8 (150 x 2.1mm,1.7um)","SOLVENT_A":"100% water; 0.1% formic acid","SOLVENT_B":"100% acetonitrile; 0.1% formic acid","FLOW_GRADIENT":"isocratic at the start (0 - 0.5 min) with 4% of B solvent, then at linear gradient (0.5 – 10 min) with 34% B and (10 – 15 min) with 100% B, and finally isocratic (15 – 18 min) with 100% B","FLOW_RATE":"400 μL min−1","COLUMN_TEMPERATURE":"40 °C"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Bruker maXis Impact qTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"For external calibration, we used a sodium formate solution (10 mM NaOH solution in 50/50 v/v isopropanol/water containing 0.2% formic acid) directly injected through a 6-port valve at the beginning of each chromatographic run. UHPLC-MS data was acquired by the HyStar Application version 3.2 (Bruker Daltonics, Germany). Data pre-processing was performed using the software DataAnalysis version 4.4 (Bruker Daltonics, Germany), where raw data from the UHPLC-MS analysis were exported as .mzXML files.","MS_RESULTS_FILE":"ST002537_AN004176_Results.txt UNITS:m/z Has m/z:Yes Has RT:No RT units:No RT data ST002537_AN004176_Results.txt UNITS:m/z Has m/z:Yes Has RT:No RT units:No RT data ST002537_AN004176_Results.txt UNITS:m/z Has m/z:Yes Has RT:No RT units:No RT data ST002537_AN004176_Results.txt UNITS:m/z Has m/z:Yes Has RT:No RT units:No RT data"}

}