#METABOLOMICS WORKBENCH tsimaom_20230620_111958 DATATRACK_ID:4104 STUDY_ID:ST002749 ANALYSIS_ID:AN004459 PROJECT_ID:PR001712 VERSION 1 CREATED_ON June 23, 2023, 5:26 pm #PROJECT PR:PROJECT_TITLE Bromeliad lipidomics for adaptation to elevation PR:PROJECT_TYPE MS untargeted analysis PR:PROJECT_SUMMARY Untargeted analysis on Bromeliades leaf samples. The aim of this study was to PR:PROJECT_SUMMARY evaluate the lipid profile alterations on Pitcairnia flammea leaves based on the PR:PROJECT_SUMMARY different altitudes where they were collected. A lipidomic approach was applied PR:PROJECT_SUMMARY to the samples. Ultra-high performance liquid chromatography coupled to PR:PROJECT_SUMMARY electrospray ionization mass spectrometry (UHPLC-ESI-MS) was used to acquire raw PR:PROJECT_SUMMARY data and MS-DIAL was used to perform data preprocessing. The statistical PR:PROJECT_SUMMARY analysis of UHPLC-ESI-MS data in both ionization modes enabled the visualization PR:PROJECT_SUMMARY of a trend distribution based on the altitude. PR:INSTITUTE University of Campinas PR:DEPARTMENT Chemistry's Institute PR:LABORATORY Laboratory of Bioanalytics and Integrated Omics PR:LAST_NAME Matos PR:FIRST_NAME Taynara PR:ADDRESS Rua Josué de Castro, s/n – Cidade Universitária, 13083-970, Campinas – SP, PR:ADDRESS Brazil PR:EMAIL t262827@dac.unicamp.br PR:PHONE (85)996154192 #STUDY ST:STUDY_TITLE Bromeliad lipidomics for adaptation to elevation ST:STUDY_SUMMARY Untargeted analysis on Bromeliades leaf samples. The aim of this study was to ST:STUDY_SUMMARY evaluate the lipid profile alterations on Pitcairnia flammea leaves based on the ST:STUDY_SUMMARY different altitudes where they were collected. A lipidomic approach was applied ST:STUDY_SUMMARY to the samples. Ultra-high performance liquid chromatography coupled to ST:STUDY_SUMMARY electrospray ionization mass spectrometry (UHPLC-ESI-MS) was used to acquire raw ST:STUDY_SUMMARY data and MS-DIAL was used to perform data preprocessing. The statistical ST:STUDY_SUMMARY analysis of UHPLC-ESI-MS data in both ionization modes enabled the visualization ST:STUDY_SUMMARY of a trend distribution based on the altitude. Our study grouped the individuals ST:STUDY_SUMMARY into three major groups: one with individuals from the lower elevations (UBA, ST:STUDY_SUMMARY RAN, and COR), another with individuals from the highest elevations (PAP and ST:STUDY_SUMMARY MAR), and another with individuals from mixed localities from intermediate ST:STUDY_SUMMARY elevations. Higher elevation population showed an increase in very long chain ST:STUDY_SUMMARY fatty acids and monounsaturated lipids compared to lower elevation as a response ST:STUDY_SUMMARY to cold higher elevations affecting the leaves' membrane fluidity. In addition, ST:STUDY_SUMMARY the higher elevation population showed a higher abundance of hexosylceramides ST:STUDY_SUMMARY and phosphatidylglycerol compared to lower ones. ST:INSTITUTE University of Campinas ST:DEPARTMENT Chemistry's Institute ST:LABORATORY Laboratory of Bioanalytics and Integrated Omics ST:LAST_NAME Matos ST:FIRST_NAME Taynara ST:ADDRESS Rua Josué de Castro, s/n – Cidade Universitária, 13083-970, Campinas – SP, ST:ADDRESS Brazil ST:EMAIL t262827@dac.unicamp.br ST:PHONE (85)996154192 #SUBJECT SU:SUBJECT_TYPE Plant SU:SUBJECT_SPECIES Pitcairnia flammea #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - COR186-NEG Collection locale:COR Sample_type=Sample; RAW_FILE_NAME=COR186-NEG SUBJECT_SAMPLE_FACTORS - COR191-NEG Collection locale:COR Sample_type=Sample; RAW_FILE_NAME=COR191-NEG SUBJECT_SAMPLE_FACTORS - COR192-NEG Collection locale:COR Sample_type=Sample; RAW_FILE_NAME=COR192-NEG SUBJECT_SAMPLE_FACTORS - COR195-NEG Collection locale:COR Sample_type=Sample; RAW_FILE_NAME=COR195-NEG SUBJECT_SAMPLE_FACTORS - COR197-NEG Collection locale:COR Sample_type=Sample; 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RAW_FILE_NAME=MAC155-NEG SUBJECT_SAMPLE_FACTORS - MAC158-NEG Collection locale:MAC Sample_type=Sample; RAW_FILE_NAME=MAC158-NEG SUBJECT_SAMPLE_FACTORS - MAC163-NEG Collection locale:MAC Sample_type=Sample; RAW_FILE_NAME=MAC163-NEG SUBJECT_SAMPLE_FACTORS - MAR128-NEG Collection locale:MAR Sample_type=Sample; RAW_FILE_NAME=MAR128-NEG SUBJECT_SAMPLE_FACTORS - MAR131-NEG Collection locale:MAR Sample_type=Sample; RAW_FILE_NAME=MAR131-NEG SUBJECT_SAMPLE_FACTORS - MAR134-NEG Collection locale:MAR Sample_type=Sample; RAW_FILE_NAME=MAR134-NEG SUBJECT_SAMPLE_FACTORS - MAR139-NEG Collection locale:MAR Sample_type=Sample; RAW_FILE_NAME=MAR139-NEG SUBJECT_SAMPLE_FACTORS - MAR155-NEG Collection locale:MAR Sample_type=Sample; RAW_FILE_NAME=MAR155-NEG SUBJECT_SAMPLE_FACTORS - PAP01-NEG Collection locale:PAP Sample_type=Sample; RAW_FILE_NAME=PAP01-NEG SUBJECT_SAMPLE_FACTORS - PAP04-NEG Collection locale:PAP Sample_type=Sample; RAW_FILE_NAME=PAP04-NEG SUBJECT_SAMPLE_FACTORS - PAP15-NEG Collection locale:PAP Sample_type=Sample; RAW_FILE_NAME=PAP15-NEG SUBJECT_SAMPLE_FACTORS - PAP17-NEG Collection locale:PAP Sample_type=Sample; RAW_FILE_NAME=PAP17-NEG SUBJECT_SAMPLE_FACTORS - PAP24-NEG Collection locale:PAP Sample_type=Sample; RAW_FILE_NAME=PAP24-NEG SUBJECT_SAMPLE_FACTORS - PES142-NEG Collection locale:PES Sample_type=Sample; RAW_FILE_NAME=PES142-NEG SUBJECT_SAMPLE_FACTORS - PES145-NEG Collection locale:PES Sample_type=Sample; RAW_FILE_NAME=PES145-NEG SUBJECT_SAMPLE_FACTORS - PES148-NEG Collection locale:PES Sample_type=Sample; RAW_FILE_NAME=PES148-NEG SUBJECT_SAMPLE_FACTORS - PES149-NEG Collection locale:PES Sample_type=Sample; RAW_FILE_NAME=PES149-NEG SUBJECT_SAMPLE_FACTORS - PES150-NEG Collection locale:PES Sample_type=Sample; RAW_FILE_NAME=PES150-NEG SUBJECT_SAMPLE_FACTORS - QC01-NEG Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC01-NEG SUBJECT_SAMPLE_FACTORS - QC02-NEG Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC02-NEG SUBJECT_SAMPLE_FACTORS - QC03-NEG Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC03-NEG SUBJECT_SAMPLE_FACTORS - QC04-NEG Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC04-NEG SUBJECT_SAMPLE_FACTORS - QC05-NEG Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC05-NEG SUBJECT_SAMPLE_FACTORS - QC06-NEG Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC06-NEG SUBJECT_SAMPLE_FACTORS - QC07-NEG Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC07-NEG SUBJECT_SAMPLE_FACTORS - QC08-NEG Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC08-NEG SUBJECT_SAMPLE_FACTORS - QC09-NEG Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC09-NEG SUBJECT_SAMPLE_FACTORS - QC010-NEG Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC010-NEG SUBJECT_SAMPLE_FACTORS - RAN167-NEG Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN167-NEG SUBJECT_SAMPLE_FACTORS - RAN169-NEG Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN169-NEG SUBJECT_SAMPLE_FACTORS - RAN173-NEG Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN173-NEG SUBJECT_SAMPLE_FACTORS - RAN174-NEG Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN174-NEG SUBJECT_SAMPLE_FACTORS - RAN175-NEG Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN175-NEG SUBJECT_SAMPLE_FACTORS - RAN177-NEG Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN177-NEG SUBJECT_SAMPLE_FACTORS - RAN178-NEG Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN178-NEG SUBJECT_SAMPLE_FACTORS - UBA01-NEG Collection locale:UBA Sample_type=Sample; RAW_FILE_NAME=UBA01-NEG SUBJECT_SAMPLE_FACTORS - UBA02-NEG Collection locale:UBA Sample_type=Sample; RAW_FILE_NAME=UBA02-NEG SUBJECT_SAMPLE_FACTORS - UBA04-NEG Collection locale:UBA Sample_type=Sample; RAW_FILE_NAME=UBA04-NEG SUBJECT_SAMPLE_FACTORS - UBA05-NEG Collection locale:UBA Sample_type=Sample; RAW_FILE_NAME=UBA05-NEG SUBJECT_SAMPLE_FACTORS - UBA06-NEG Collection locale:UBA Sample_type=Sample; RAW_FILE_NAME=UBA06-NEG SUBJECT_SAMPLE_FACTORS - UBA08-NEG Collection locale:UBA Sample_type=Sample; RAW_FILE_NAME=UBA08-NEG SUBJECT_SAMPLE_FACTORS - COR186-POS Collection locale:COR Sample_type=Sample; RAW_FILE_NAME=COR186-POS SUBJECT_SAMPLE_FACTORS - COR191-POS Collection locale:COR Sample_type=Sample; RAW_FILE_NAME=COR191-POS SUBJECT_SAMPLE_FACTORS - COR192-POS Collection locale:COR Sample_type=Sample; RAW_FILE_NAME=COR192-POS SUBJECT_SAMPLE_FACTORS - COR195-POS Collection locale:COR Sample_type=Sample; RAW_FILE_NAME=COR195-POS SUBJECT_SAMPLE_FACTORS - COR197-POS Collection locale:COR Sample_type=Sample; RAW_FILE_NAME=COR197-POS SUBJECT_SAMPLE_FACTORS - COR199-POS Collection locale:COR Sample_type=Sample; RAW_FILE_NAME=COR199-POS SUBJECT_SAMPLE_FACTORS - ITA102-POS Collection locale:ITA Sample_type=Sample; RAW_FILE_NAME=ITA102-POS SUBJECT_SAMPLE_FACTORS - ITA110-POS Collection locale:ITA Sample_type=Sample; RAW_FILE_NAME=ITA110-POS SUBJECT_SAMPLE_FACTORS - ITA113-POS Collection locale:ITA Sample_type=Sample; RAW_FILE_NAME=ITA113-POS SUBJECT_SAMPLE_FACTORS - ITA115-POS Collection locale:ITA Sample_type=Sample; RAW_FILE_NAME=ITA115-POS SUBJECT_SAMPLE_FACTORS - ITA116-POS Collection locale:ITA Sample_type=Sample; RAW_FILE_NAME=ITA116-POS SUBJECT_SAMPLE_FACTORS - ITA119-POS Collection locale:ITA Sample_type=Sample; RAW_FILE_NAME=ITA119-POS SUBJECT_SAMPLE_FACTORS - MAC152-POS Collection locale:MAC Sample_type=Sample; RAW_FILE_NAME=MAC152-POS SUBJECT_SAMPLE_FACTORS - MAC153-POS Collection locale:MAC Sample_type=Sample; RAW_FILE_NAME=MAC153-POS SUBJECT_SAMPLE_FACTORS - MAC155-POS Collection locale:MAC Sample_type=Sample; RAW_FILE_NAME=MAC155-POS SUBJECT_SAMPLE_FACTORS - MAC158-POS Collection locale:MAC Sample_type=Sample; RAW_FILE_NAME=MAC158-POS SUBJECT_SAMPLE_FACTORS - MAC163-POS Collection locale:MAC Sample_type=Sample; RAW_FILE_NAME=MAC163-POS SUBJECT_SAMPLE_FACTORS - MAR128-POS Collection locale:MAR Sample_type=Sample; RAW_FILE_NAME=MAR128-POS SUBJECT_SAMPLE_FACTORS - MAR131-POS Collection locale:MAR Sample_type=Sample; RAW_FILE_NAME=MAR131-POS SUBJECT_SAMPLE_FACTORS - MAR134-POS Collection locale:MAR Sample_type=Sample; RAW_FILE_NAME=MAR134-POS SUBJECT_SAMPLE_FACTORS - MAR139-POS Collection locale:MAR Sample_type=Sample; RAW_FILE_NAME=MAR139-POS SUBJECT_SAMPLE_FACTORS - MAR155-POS Collection locale:MAR Sample_type=Sample; RAW_FILE_NAME=MAR155-POS SUBJECT_SAMPLE_FACTORS - PAP01-POS Collection locale:PAP Sample_type=Sample; RAW_FILE_NAME=PAP01-POS SUBJECT_SAMPLE_FACTORS - PAP04-POS Collection locale:PAP Sample_type=Sample; RAW_FILE_NAME=PAP04-POS SUBJECT_SAMPLE_FACTORS - PAP15-POS Collection locale:PAP Sample_type=Sample; RAW_FILE_NAME=PAP15-POS SUBJECT_SAMPLE_FACTORS - PAP17-POS Collection locale:PAP Sample_type=Sample; RAW_FILE_NAME=PAP17-POS SUBJECT_SAMPLE_FACTORS - PAP24-POS Collection locale:PAP Sample_type=Sample; RAW_FILE_NAME=PAP24-POS SUBJECT_SAMPLE_FACTORS - PES142-POS Collection locale:PES Sample_type=Sample; RAW_FILE_NAME=PES142-POS SUBJECT_SAMPLE_FACTORS - PES145-POS Collection locale:PES Sample_type=Sample; RAW_FILE_NAME=PES145-POS SUBJECT_SAMPLE_FACTORS - PES148-POS Collection locale:PES Sample_type=Sample; RAW_FILE_NAME=PES148-POS SUBJECT_SAMPLE_FACTORS - PES149-POS Collection locale:PES Sample_type=Sample; RAW_FILE_NAME=PES149-POS SUBJECT_SAMPLE_FACTORS - PES150-POS Collection locale:PES Sample_type=Sample; RAW_FILE_NAME=PES150-POS SUBJECT_SAMPLE_FACTORS - QC01-POS Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC01-POS SUBJECT_SAMPLE_FACTORS - QC02-POS Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC02-POS SUBJECT_SAMPLE_FACTORS - QC03-POS Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC03-POS SUBJECT_SAMPLE_FACTORS - QC04-POS Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC04-POS SUBJECT_SAMPLE_FACTORS - QC05-POS Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC05-POS SUBJECT_SAMPLE_FACTORS - QC06-POS Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC06-POS SUBJECT_SAMPLE_FACTORS - QC07-POS Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC07-POS SUBJECT_SAMPLE_FACTORS - QC08-POS Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC08-POS SUBJECT_SAMPLE_FACTORS - QC09-POS Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC09-POS SUBJECT_SAMPLE_FACTORS - QC010-POS Collection locale:Pooled QC Sample_type=Quality Control (QC); RAW_FILE_NAME=QC010-POS SUBJECT_SAMPLE_FACTORS - RAN167-POS Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN167-POS SUBJECT_SAMPLE_FACTORS - RAN169-POS Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN169-POS SUBJECT_SAMPLE_FACTORS - RAN173-POS Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN173-POS SUBJECT_SAMPLE_FACTORS - RAN174-POS Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN174-POS SUBJECT_SAMPLE_FACTORS - RAN175-POS Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN175-POS SUBJECT_SAMPLE_FACTORS - RAN177-POS Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN177-POS SUBJECT_SAMPLE_FACTORS - RAN178-POS Collection locale:RAN Sample_type=Sample; RAW_FILE_NAME=RAN178-POS SUBJECT_SAMPLE_FACTORS - UBA01-POS Collection locale:UBA Sample_type=Sample; RAW_FILE_NAME=UBA01-POS SUBJECT_SAMPLE_FACTORS - UBA02-POS Collection locale:UBA Sample_type=Sample; RAW_FILE_NAME=UBA02-POS SUBJECT_SAMPLE_FACTORS - UBA04-POS Collection locale:UBA Sample_type=Sample; RAW_FILE_NAME=UBA04-POS SUBJECT_SAMPLE_FACTORS - UBA05-POS Collection locale:UBA Sample_type=Sample; RAW_FILE_NAME=UBA05-POS SUBJECT_SAMPLE_FACTORS - UBA06-POS Collection locale:UBA Sample_type=Sample; RAW_FILE_NAME=UBA06-POS SUBJECT_SAMPLE_FACTORS - UBA08-POS Collection locale:UBA Sample_type=Sample; RAW_FILE_NAME=UBA08-POS #COLLECTION CO:COLLECTION_SUMMARY Pitcairnia flammea individuals were collected in different altitudes locations CO:COLLECTION_SUMMARY and named according to the local. PAP was collected at 2140 m, MAR 2037 m, PES CO:COLLECTION_SUMMARY 1496 m, ITA 1139 m, MAC 959 m, COR 425 m, RAN 25 m and UBA 20 m. Then, the CO:COLLECTION_SUMMARY plants were cultivated at the Institute of Biology (UNICAMP, Brazil). The CO:COLLECTION_SUMMARY mid-region of the youngest fully expanded leaves of five to seven individuals CO:COLLECTION_SUMMARY per population of healthy plants with visually appealing leaves were harvested, CO:COLLECTION_SUMMARY and immediately frozen in liquid nitrogen, followed the -80ºC storage until CO:COLLECTION_SUMMARY sample preparation. CO:SAMPLE_TYPE Leaves CO:COLLECTION_LOCATION Institute of Biology (UNICAMP, Brazil) #TREATMENT TR:TREATMENT_SUMMARY Leaves were macerated to fine powder using a mortar and pestle in liquid TR:TREATMENT_SUMMARY nitrogen condition. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Lipid extraction was based on Hummel et al. Method. 60 mL of solvent mixture was SP:SAMPLEPREP_SUMMARY prepared with pre-cooled (-20ºC) methanol (MeOH, grade HPLC, LiChrosolv® Reag. SP:SAMPLEPREP_SUMMARY Ph. Eur.) and methyl-tert-butyl-ether (MTBE, grade HPLC, purity 99,9%, SP:SAMPLEPREP_SUMMARY Sigma-Aldrich) in proportion (1:3 v/v). In a 2 mL tube, 50 mg of macerated SP:SAMPLEPREP_SUMMARY sample were added and 1 mL of the solvent mixture. The samples were incubated SP:SAMPLEPREP_SUMMARY for 5 min under agitation at 500 rpm at 4 ºC (Microtube Shaking Incubator SP:SAMPLEPREP_SUMMARY AccuTherm, Labnet International, Inc.), followed by an ultrasonication (Branson SP:SAMPLEPREP_SUMMARY 5800 Ultrasonic Bath, Emerson, Danbury, USA) in ice-cold bath in 10 minutes. SP:SAMPLEPREP_SUMMARY After adding 500 μL mixture of water type I:MeOH (3:1 v/v), the samples were SP:SAMPLEPREP_SUMMARY vortexed and centrifuged for 5 min at 4 ºC, 10000 rpm (Hettich Zentrifugen SP:SAMPLEPREP_SUMMARY Mikro 220R, Tuttlingen, DE). The three phases were separated and dried in a SP:SAMPLEPREP_SUMMARY vacuum concentrator (Concentrator Plus, Eppendorf AG, Hamburg, DE), at ambient SP:SAMPLEPREP_SUMMARY temperature under vacuum - alcoholic mode, and stored at -80 ºC until the SP:SAMPLEPREP_SUMMARY chromatographic analysis. SP:SAMPLEPREP_PROTOCOL_FILENAME SamplePrep-BromeliadLipidomics.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Scientific UltiMate™ 3000 UHPLC RSLCnano system CH:COLUMN_NAME Supelco Sigma-Aldrich Titan C18 (100 x 2.1mm, 1.9um) CH:SOLVENT_A 40% acetonitrile/60% water; 10 mM ammonium acetate CH:SOLVENT_B 10% acetonitrile/90% isopropanol; 10 mM ammonium acetate CH:FLOW_GRADIENT 250 µL/min: 0-2 min: 40% B, 2-3 min: 50% B, 3-6 min: 50% B, 6.1-8 min: 70% B, CH:FLOW_GRADIENT 8-9 min: 100% B, 9-11 min: 100% B, 11-12 min: 40% B, 12-14 min: 40% B. CH:FLOW_RATE 250 µL/min CH:COLUMN_TEMPERATURE 40 CH:METHODS_FILENAME UntargetedRP-BromeliadLipidomics.pdf #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE UntargetedRP-BromeliadLipidomics.pdf #MS MS:INSTRUMENT_NAME Thermo Q Exactive Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS - MS:MS_RESULTS_FILE ST002749_AN004459_Results.txt UNITS:Peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END