{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002749","ANALYSIS_ID":"AN004460","VERSION":"1","CREATED_ON":"June 23, 2023, 5:26 pm"},

"PROJECT":{"PROJECT_TITLE":"Bromeliad lipidomics for adaptation to elevation","PROJECT_TYPE":"MS untargeted analysis","PROJECT_SUMMARY":"Untargeted analysis on Bromeliades leaf samples. The aim of this study was to evaluate the lipid profile alterations on Pitcairnia flammea leaves based on the different altitudes where they were collected. A lipidomic approach was applied to the samples. Ultra-high performance liquid chromatography coupled to electrospray ionization mass spectrometry (UHPLC-ESI-MS) was used to acquire raw data and MS-DIAL was used to perform data preprocessing. The statistical analysis of UHPLC-ESI-MS data in both ionization modes enabled the visualization of a trend distribution based on the altitude.","INSTITUTE":"University of Campinas","DEPARTMENT":"Chemistry's Institute","LABORATORY":"Laboratory of Bioanalytics and Integrated Omics","LAST_NAME":"Matos","FIRST_NAME":"Taynara","ADDRESS":"Rua Josué de Castro, s/n – Cidade Universitária, 13083-970, Campinas – SP, Brazil","EMAIL":"t262827@dac.unicamp.br","PHONE":"(85)996154192"},

"STUDY":{"STUDY_TITLE":"Bromeliad lipidomics for adaptation to elevation","STUDY_SUMMARY":"Untargeted analysis on Bromeliades leaf samples. The aim of this study was to evaluate the lipid profile alterations on Pitcairnia flammea leaves based on the different altitudes where they were collected. A lipidomic approach was applied to the samples. Ultra-high performance liquid chromatography coupled to electrospray ionization mass spectrometry (UHPLC-ESI-MS) was used to acquire raw data and MS-DIAL was used to perform data preprocessing. The statistical analysis of UHPLC-ESI-MS data in both ionization modes enabled the visualization of a trend distribution based on the altitude. Our study grouped the individuals into three major groups: one with individuals from the lower elevations (UBA, RAN, and COR), another with individuals from the highest elevations (PAP and MAR), and another with individuals from mixed localities from intermediate elevations. Higher elevation population showed an increase in very long chain fatty acids and monounsaturated lipids compared to lower elevation as a response to cold higher elevations affecting the leaves' membrane fluidity. In addition, the higher elevation population showed a higher abundance of hexosylceramides and phosphatidylglycerol compared to lower ones.","INSTITUTE":"University of Campinas","DEPARTMENT":"Chemistry's Institute","LABORATORY":"Laboratory of Bioanalytics and Integrated Omics","LAST_NAME":"Matos","FIRST_NAME":"Taynara","ADDRESS":"Rua Josué de Castro, s/n – Cidade Universitária, 13083-970, Campinas – SP, Brazil","EMAIL":"t262827@dac.unicamp.br","PHONE":"(85)996154192"},

"SUBJECT":{"SUBJECT_TYPE":"Plant","SUBJECT_SPECIES":"Pitcairnia flammea"},
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],
"COLLECTION":{"COLLECTION_SUMMARY":"Pitcairnia flammea individuals were collected in different altitudes locations and named according to the local. PAP was collected at 2140 m, MAR 2037 m, PES 1496 m, ITA 1139 m, MAC 959 m, COR 425 m, RAN 25 m and UBA 20 m. Then, the plants were cultivated at the Institute of Biology (UNICAMP, Brazil). The mid-region of the youngest fully expanded leaves of five to seven individuals per population of healthy plants with visually appealing leaves were harvested, and immediately frozen in liquid nitrogen, followed the -80ºC storage until sample preparation.","SAMPLE_TYPE":"Leaves","COLLECTION_LOCATION":"Institute of Biology (UNICAMP, Brazil)"},

"TREATMENT":{"TREATMENT_SUMMARY":"Leaves were macerated to fine powder using a mortar and pestle in liquid nitrogen condition."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Lipid extraction was based on Hummel et al. Method. 60 mL of solvent mixture was prepared with pre-cooled (-20ºC) methanol (MeOH, grade HPLC, LiChrosolv® Reag. Ph. Eur.) and methyl-tert-butyl-ether (MTBE, grade HPLC, purity 99,9%, Sigma-Aldrich) in proportion (1:3 v/v). In a 2 mL tube, 50 mg of macerated sample were added and 1 mL of the solvent mixture. The samples were incubated for 5 min under agitation at 500 rpm at 4 ºC (Microtube Shaking Incubator AccuTherm, Labnet International, Inc.), followed by an ultrasonication (Branson 5800 Ultrasonic Bath, Emerson, Danbury, USA) in ice-cold bath in 10 minutes. After adding 500 μL mixture of water type I:MeOH (3:1 v/v), the samples were vortexed and centrifuged for 5 min at 4 ºC, 10000 rpm (Hettich Zentrifugen Mikro 220R, Tuttlingen, DE). The three phases were separated and dried in a vacuum concentrator (Concentrator Plus, Eppendorf AG, Hamburg, DE), at ambient temperature under vacuum - alcoholic mode, and stored at -80 ºC until the chromatographic analysis.","SAMPLEPREP_PROTOCOL_FILENAME":"SamplePrep-BromeliadLipidomics.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Scientific UltiMate™ 3000 UHPLC RSLCnano system","COLUMN_NAME":"Supelco Sigma-Aldrich Titan C18 (100 x 2.1mm, 1.9um)","SOLVENT_A":"40% acetonitrile/60% water; 10 mM ammonium acetate","SOLVENT_B":"10% acetonitrile/90% isopropanol; 10 mM ammonium acetate","FLOW_GRADIENT":"250 µL/min: 0-2 min: 40% B, 2-3 min: 50% B, 3-6 min: 50% B, 6.1-8 min: 70% B, 8-9 min: 100% B, 9-11 min: 100% B, 11-12 min: 40% B, 12-14 min: 40% B.","FLOW_RATE":"250 µL/min","COLUMN_TEMPERATURE":"40","METHODS_FILENAME":"UntargetedRP-BromeliadLipidomics.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"UntargetedRP-BromeliadLipidomics.pdf"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"-","MS_RESULTS_FILE":"ST002749_AN004460_Results.txt UNITS:Peak area Has m/z:Yes Has RT:Yes RT units:Minutes"}

}