{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002757","ANALYSIS_ID":"AN004474","VERSION":"1","CREATED_ON":"June 21, 2023, 8:49 am"}, "PROJECT":{"PROJECT_TITLE":"The ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (CIOB Cohort)","PROJECT_TYPE":"C18 Reversed-Phase Broad Spectrum Metabolomics","PROJECT_SUMMARY":"This project was funded by the NIH Environmental influences on Child Health Outcomes (ECHO) Program. Untargeted metabolomics profiling data was acquired from urine samples provided by 14 ECHO cohorts.","INSTITUTE":"NC HHEAR Hub","DEPARTMENT":"Untargeted Analysis","LABORATORY":"Sumner Lab","LAST_NAME":"Rushing","FIRST_NAME":"Blake","ADDRESS":"Nutrition Research Institute , University of North Carolina at Chapel Hill, 500 Laureate Way Kannapolis, NC 28081","EMAIL":"blake_rushing@unc.edu","PHONE":"(704) 282-9838","FUNDING_SOURCE":"This research was supported by the Environmental influences on Child Health Outcomes (ECHO) Program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The CIOB ECHO Cohort is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier.D U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539"}, "STUDY":{"STUDY_TITLE":"The ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (CIOB Cohort)","STUDY_TYPE":"Prospective Cohort Study","STUDY_SUMMARY":"CIOB is a prospective prenatal cohort study measuring maternal exposures to endocrine disrupting chemicals and place-based, perceptual, and biological markers of chronic psychosocial stressors and their relationships to adverse pregnancy outcomes and neurodevelopment in children. We have recruited nearly 800 pregnant participants from two locations in San Francisco; Zuckerberg San Francisco General Hospital (ZSFG), which serves primarily low income and Latinx patients, and Mission Bay Hospital (MB), which serves higher income and primarily white and Asian patients. We recruited women during their 2nd trimester (13 – 27 weeks) and collected biospecimens (urine, blood, hair, nails) and questionnaires during in-person visits, and umbilical cord and maternal blood and urine at delivery. We measured multiple classes of chemical exposures in our participants including flame retardants, per- and poly-fluoroalkyl substances (PFAS), phthalates, pesticides, phenols, aromatic amines, and we collaborated on development and implementation of shared standardized core data elements, questionnaires at multiple time points during pregnancy and childhood, and collection of biospecimens for chemical and mechanistic analyses during pregnancy and childhood. Please contact Erin DeMicco at erin.demicco@ucsf.edu for questions related to the subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) Program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. CIOB is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier.D U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539","INSTITUTE":"University of California, San Francisco","DEPARTMENT":"School of Medicine","LAST_NAME":"Woodruff","FIRST_NAME":"Tracey","ADDRESS":"490 Illinois Street San Francisco CA 94158","EMAIL":"Tracey.Woodruff@ucsf.edu","PHONE":"(415) 476-3198","TOTAL_SUBJECTS":"463","STUDY_COMMENTS":"HHEAR Project EM20-0011, ECHO Project EC0376"}, "SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"CIOB_B_1", "Factors":{"Sample Type":"Blank","Batch":"1"}, "Additional sample data":{"Analysis Order":"12","RAW_FILE_NAME":"CIOB_B_1"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_2", "Factors":{"Sample Type":"Blank","Batch":"1"}, "Additional sample data":{"Analysis Order":"26","RAW_FILE_NAME":"CIOB_B_2"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_3", "Factors":{"Sample Type":"Blank","Batch":"1"}, "Additional sample data":{"Analysis Order":"39","RAW_FILE_NAME":"CIOB_B_3"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_4", "Factors":{"Sample Type":"Blank","Batch":"1"}, "Additional sample data":{"Analysis Order":"53","RAW_FILE_NAME":"CIOB_B_4"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_5", "Factors":{"Sample Type":"Blank","Batch":"1"}, "Additional sample data":{"Analysis Order":"67","RAW_FILE_NAME":"CIOB_B_5"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_6", "Factors":{"Sample Type":"Blank","Batch":"1"}, "Additional sample data":{"Analysis Order":"81","RAW_FILE_NAME":"CIOB_B_6"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_7", "Factors":{"Sample Type":"Blank","Batch":"1"}, "Additional sample data":{"Analysis Order":"94","RAW_FILE_NAME":"CIOB_B_7"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_8", "Factors":{"Sample Type":"Blank","Batch":"1"}, "Additional sample data":{"Analysis Order":"108","RAW_FILE_NAME":"CIOB_B_8"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_9", "Factors":{"Sample Type":"Blank","Batch":"2"}, "Additional sample data":{"Analysis Order":"123","RAW_FILE_NAME":"CIOB_B_9"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_10", "Factors":{"Sample Type":"Blank","Batch":"2"}, "Additional sample data":{"Analysis Order":"137","RAW_FILE_NAME":"CIOB_B_10"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_11", "Factors":{"Sample Type":"Blank","Batch":"2"}, "Additional sample data":{"Analysis Order":"150","RAW_FILE_NAME":"CIOB_B_11"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_12", "Factors":{"Sample Type":"Blank","Batch":"2"}, "Additional sample data":{"Analysis Order":"164","RAW_FILE_NAME":"CIOB_B_12"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_13", "Factors":{"Sample Type":"Blank","Batch":"2"}, "Additional sample data":{"Analysis Order":"178","RAW_FILE_NAME":"CIOB_B_13"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_14", "Factors":{"Sample Type":"Blank","Batch":"2"}, "Additional sample data":{"Analysis Order":"192","RAW_FILE_NAME":"CIOB_B_14"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_15", "Factors":{"Sample Type":"Blank","Batch":"2"}, "Additional sample data":{"Analysis Order":"205","RAW_FILE_NAME":"CIOB_B_15"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_16", "Factors":{"Sample Type":"Blank","Batch":"2"}, "Additional sample data":{"Analysis Order":"219","RAW_FILE_NAME":"CIOB_B_16"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_17", "Factors":{"Sample Type":"Blank","Batch":"3"}, "Additional sample data":{"Analysis Order":"234","RAW_FILE_NAME":"CIOB_B_17"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_18", "Factors":{"Sample Type":"Blank","Batch":"3"}, "Additional sample data":{"Analysis Order":"248","RAW_FILE_NAME":"CIOB_B_18"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_19", "Factors":{"Sample Type":"Blank","Batch":"3"}, "Additional sample data":{"Analysis Order":"261","RAW_FILE_NAME":"CIOB_B_19"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_20", "Factors":{"Sample Type":"Blank","Batch":"3"}, "Additional sample data":{"Analysis Order":"275","RAW_FILE_NAME":"CIOB_B_20"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_21", "Factors":{"Sample Type":"Blank","Batch":"3"}, "Additional sample data":{"Analysis Order":"289","RAW_FILE_NAME":"CIOB_B_21"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_22", "Factors":{"Sample Type":"Blank","Batch":"3"}, "Additional sample data":{"Analysis Order":"303","RAW_FILE_NAME":"CIOB_B_22"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_23", "Factors":{"Sample Type":"Blank","Batch":"3"}, "Additional sample data":{"Analysis Order":"316","RAW_FILE_NAME":"CIOB_B_23"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_24", "Factors":{"Sample Type":"Blank","Batch":"3"}, "Additional sample data":{"Analysis Order":"330","RAW_FILE_NAME":"CIOB_B_24"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_25", "Factors":{"Sample Type":"Blank","Batch":"4"}, "Additional sample data":{"Analysis Order":"345","RAW_FILE_NAME":"CIOB_B_25"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_26", "Factors":{"Sample Type":"Blank","Batch":"4"}, "Additional sample data":{"Analysis Order":"359","RAW_FILE_NAME":"CIOB_B_26"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_27", "Factors":{"Sample Type":"Blank","Batch":"4"}, "Additional sample data":{"Analysis Order":"372","RAW_FILE_NAME":"CIOB_B_27"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_28", "Factors":{"Sample Type":"Blank","Batch":"4"}, "Additional sample data":{"Analysis Order":"386","RAW_FILE_NAME":"CIOB_B_28"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_29", "Factors":{"Sample Type":"Blank","Batch":"4"}, "Additional sample data":{"Analysis Order":"400","RAW_FILE_NAME":"CIOB_B_29"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_30", "Factors":{"Sample Type":"Blank","Batch":"4"}, "Additional sample data":{"Analysis Order":"414","RAW_FILE_NAME":"CIOB_B_30"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_31", "Factors":{"Sample Type":"Blank","Batch":"4"}, "Additional sample data":{"Analysis Order":"427","RAW_FILE_NAME":"CIOB_B_31"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_32", "Factors":{"Sample Type":"Blank","Batch":"4"}, "Additional sample data":{"Analysis Order":"441","RAW_FILE_NAME":"CIOB_B_32"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_33", "Factors":{"Sample Type":"Blank","Batch":"5"}, "Additional sample data":{"Analysis Order":"456","RAW_FILE_NAME":"CIOB_B_33"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_34", "Factors":{"Sample Type":"Blank","Batch":"5"}, "Additional sample data":{"Analysis Order":"470","RAW_FILE_NAME":"CIOB_B_34"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_35", "Factors":{"Sample Type":"Blank","Batch":"5"}, "Additional sample data":{"Analysis Order":"483","RAW_FILE_NAME":"CIOB_B_35"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_36", "Factors":{"Sample Type":"Blank","Batch":"5"}, "Additional sample data":{"Analysis Order":"497","RAW_FILE_NAME":"CIOB_B_36"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_37", "Factors":{"Sample Type":"Blank","Batch":"5"}, "Additional sample data":{"Analysis Order":"511","RAW_FILE_NAME":"CIOB_B_37"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_38", "Factors":{"Sample Type":"Blank","Batch":"5"}, "Additional sample data":{"Analysis Order":"525","RAW_FILE_NAME":"CIOB_B_38"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_39", "Factors":{"Sample Type":"Blank","Batch":"5"}, "Additional sample data":{"Analysis Order":"538","RAW_FILE_NAME":"CIOB_B_39"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_40", "Factors":{"Sample Type":"Blank","Batch":"5"}, "Additional sample data":{"Analysis Order":"552","RAW_FILE_NAME":"CIOB_B_40"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_41", "Factors":{"Sample Type":"Blank","Batch":"6"}, "Additional sample data":{"Analysis Order":"567","RAW_FILE_NAME":"CIOB_B_41"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_42", "Factors":{"Sample Type":"Blank","Batch":"6"}, "Additional sample data":{"Analysis Order":"581","RAW_FILE_NAME":"CIOB_B_42"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_43", "Factors":{"Sample Type":"Blank","Batch":"6"}, "Additional sample data":{"Analysis Order":"594","RAW_FILE_NAME":"CIOB_B_43"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_44", "Factors":{"Sample Type":"Blank","Batch":"6"}, "Additional sample data":{"Analysis Order":"608","RAW_FILE_NAME":"CIOB_B_44"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_45", "Factors":{"Sample Type":"Blank","Batch":"6"}, "Additional sample data":{"Analysis Order":"622","RAW_FILE_NAME":"CIOB_B_45"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_46", "Factors":{"Sample Type":"Blank","Batch":"6"}, "Additional sample data":{"Analysis Order":"636","RAW_FILE_NAME":"CIOB_B_46"} }, { "Subject ID":"-", "Sample ID":"CIOB_B_47", "Factors":{"Sample Type":"Blank","Batch":"6"}, "Additional sample data":{"Analysis Order":"642","RAW_FILE_NAME":"CIOB_B_47"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_1", "Factors":{"Sample Type":"CHEAR","Batch":"1"}, "Additional sample data":{"Analysis Order":"14","RAW_FILE_NAME":"CIOB_CHEAR_U_1"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_2", "Factors":{"Sample Type":"CHEAR","Batch":"1"}, "Additional sample data":{"Analysis Order":"41","RAW_FILE_NAME":"CIOB_CHEAR_U_2"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_3", "Factors":{"Sample Type":"CHEAR","Batch":"1"}, "Additional sample data":{"Analysis Order":"69","RAW_FILE_NAME":"CIOB_CHEAR_U_3"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_4", "Factors":{"Sample Type":"CHEAR","Batch":"1"}, "Additional sample data":{"Analysis Order":"96","RAW_FILE_NAME":"CIOB_CHEAR_U_4"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_5", "Factors":{"Sample Type":"CHEAR","Batch":"2"}, "Additional sample data":{"Analysis Order":"125","RAW_FILE_NAME":"CIOB_CHEAR_U_5"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_6", "Factors":{"Sample Type":"CHEAR","Batch":"2"}, "Additional sample data":{"Analysis Order":"152","RAW_FILE_NAME":"CIOB_CHEAR_U_6"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_7", "Factors":{"Sample Type":"CHEAR","Batch":"2"}, "Additional sample data":{"Analysis Order":"180","RAW_FILE_NAME":"CIOB_CHEAR_U_7"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_8", "Factors":{"Sample Type":"CHEAR","Batch":"2"}, "Additional sample data":{"Analysis Order":"207","RAW_FILE_NAME":"CIOB_CHEAR_U_8"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_9", "Factors":{"Sample Type":"CHEAR","Batch":"3"}, "Additional sample data":{"Analysis Order":"236","RAW_FILE_NAME":"CIOB_CHEAR_U_9"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_10", "Factors":{"Sample Type":"CHEAR","Batch":"3"}, "Additional sample data":{"Analysis Order":"263","RAW_FILE_NAME":"CIOB_CHEAR_U_10"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_11", "Factors":{"Sample Type":"CHEAR","Batch":"3"}, "Additional sample data":{"Analysis Order":"291","RAW_FILE_NAME":"CIOB_CHEAR_U_11"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_12", "Factors":{"Sample Type":"CHEAR","Batch":"3"}, "Additional sample data":{"Analysis Order":"318","RAW_FILE_NAME":"CIOB_CHEAR_U_12"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_13", "Factors":{"Sample Type":"CHEAR","Batch":"4"}, "Additional sample data":{"Analysis Order":"347","RAW_FILE_NAME":"CIOB_CHEAR_U_13"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_14", "Factors":{"Sample Type":"CHEAR","Batch":"4"}, "Additional sample data":{"Analysis Order":"374","RAW_FILE_NAME":"CIOB_CHEAR_U_14"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_15", "Factors":{"Sample Type":"CHEAR","Batch":"4"}, "Additional sample data":{"Analysis Order":"402","RAW_FILE_NAME":"CIOB_CHEAR_U_15"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_16", "Factors":{"Sample Type":"CHEAR","Batch":"4"}, "Additional sample data":{"Analysis Order":"429","RAW_FILE_NAME":"CIOB_CHEAR_U_16"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_17", "Factors":{"Sample Type":"CHEAR","Batch":"5"}, "Additional sample data":{"Analysis Order":"458","RAW_FILE_NAME":"CIOB_CHEAR_U_17"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_18", "Factors":{"Sample Type":"CHEAR","Batch":"5"}, "Additional sample data":{"Analysis Order":"485","RAW_FILE_NAME":"CIOB_CHEAR_U_18"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_19", "Factors":{"Sample Type":"CHEAR","Batch":"5"}, "Additional sample data":{"Analysis Order":"513","RAW_FILE_NAME":"CIOB_CHEAR_U_19"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_20", "Factors":{"Sample Type":"CHEAR","Batch":"5"}, "Additional sample data":{"Analysis Order":"540","RAW_FILE_NAME":"CIOB_CHEAR_U_20"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_21", "Factors":{"Sample Type":"CHEAR","Batch":"6"}, "Additional sample data":{"Analysis Order":"569","RAW_FILE_NAME":"CIOB_CHEAR_U_21"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_22", "Factors":{"Sample Type":"CHEAR","Batch":"6"}, "Additional sample data":{"Analysis Order":"596","RAW_FILE_NAME":"CIOB_CHEAR_U_22"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_23", "Factors":{"Sample Type":"CHEAR","Batch":"6"}, "Additional sample data":{"Analysis Order":"624","RAW_FILE_NAME":"CIOB_CHEAR_U_23"} }, { "Subject ID":"-", "Sample ID":"CIOB_CHEAR_U_24", "Factors":{"Sample Type":"CHEAR","Batch":"6"}, "Additional sample data":{"Analysis Order":"644","RAW_FILE_NAME":"CIOB_CHEAR_U_24"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_1", "Factors":{"Sample Type":"HHEAR U1","Batch":"1"}, "Additional sample data":{"Analysis Order":"13","RAW_FILE_NAME":"CIOB_HHEAR_U1_1"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_2", "Factors":{"Sample Type":"HHEAR U1","Batch":"1"}, "Additional sample data":{"Analysis Order":"40","RAW_FILE_NAME":"CIOB_HHEAR_U1_2"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_3", "Factors":{"Sample Type":"HHEAR U1","Batch":"1"}, "Additional sample data":{"Analysis Order":"68","RAW_FILE_NAME":"CIOB_HHEAR_U1_3"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_4", "Factors":{"Sample Type":"HHEAR U1","Batch":"1"}, "Additional sample data":{"Analysis Order":"95","RAW_FILE_NAME":"CIOB_HHEAR_U1_4"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_5", "Factors":{"Sample Type":"HHEAR U1","Batch":"2"}, "Additional sample data":{"Analysis Order":"124","RAW_FILE_NAME":"CIOB_HHEAR_U1_5"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_6", "Factors":{"Sample Type":"HHEAR U1","Batch":"2"}, "Additional sample data":{"Analysis Order":"151","RAW_FILE_NAME":"CIOB_HHEAR_U1_6"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_7", "Factors":{"Sample Type":"HHEAR U1","Batch":"2"}, "Additional sample data":{"Analysis Order":"179","RAW_FILE_NAME":"CIOB_HHEAR_U1_7"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_8", "Factors":{"Sample Type":"HHEAR U1","Batch":"2"}, "Additional sample data":{"Analysis Order":"206","RAW_FILE_NAME":"CIOB_HHEAR_U1_8"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_9", "Factors":{"Sample Type":"HHEAR U1","Batch":"3"}, "Additional sample data":{"Analysis Order":"235","RAW_FILE_NAME":"CIOB_HHEAR_U1_9"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_10", "Factors":{"Sample Type":"HHEAR U1","Batch":"3"}, "Additional sample data":{"Analysis Order":"262","RAW_FILE_NAME":"CIOB_HHEAR_U1_10"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_11", "Factors":{"Sample Type":"HHEAR U1","Batch":"3"}, "Additional sample data":{"Analysis Order":"290","RAW_FILE_NAME":"CIOB_HHEAR_U1_11"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_12", "Factors":{"Sample Type":"HHEAR U1","Batch":"3"}, "Additional sample data":{"Analysis Order":"317","RAW_FILE_NAME":"CIOB_HHEAR_U1_12"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_13", "Factors":{"Sample Type":"HHEAR U1","Batch":"4"}, "Additional sample data":{"Analysis Order":"346","RAW_FILE_NAME":"CIOB_HHEAR_U1_13"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_14", "Factors":{"Sample Type":"HHEAR U1","Batch":"4"}, "Additional sample data":{"Analysis Order":"373","RAW_FILE_NAME":"CIOB_HHEAR_U1_14"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_15", "Factors":{"Sample Type":"HHEAR U1","Batch":"4"}, "Additional sample data":{"Analysis Order":"401","RAW_FILE_NAME":"CIOB_HHEAR_U1_15"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_16", "Factors":{"Sample Type":"HHEAR U1","Batch":"4"}, "Additional sample data":{"Analysis Order":"428","RAW_FILE_NAME":"CIOB_HHEAR_U1_16"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_17", "Factors":{"Sample Type":"HHEAR U1","Batch":"5"}, "Additional sample data":{"Analysis Order":"457","RAW_FILE_NAME":"CIOB_HHEAR_U1_17"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_18", "Factors":{"Sample Type":"HHEAR U1","Batch":"5"}, "Additional sample data":{"Analysis Order":"484","RAW_FILE_NAME":"CIOB_HHEAR_U1_18"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_19", "Factors":{"Sample Type":"HHEAR U1","Batch":"5"}, "Additional sample data":{"Analysis Order":"512","RAW_FILE_NAME":"CIOB_HHEAR_U1_19"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_20", "Factors":{"Sample Type":"HHEAR U1","Batch":"5"}, "Additional sample data":{"Analysis Order":"539","RAW_FILE_NAME":"CIOB_HHEAR_U1_20"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_21", "Factors":{"Sample Type":"HHEAR U1","Batch":"6"}, "Additional sample data":{"Analysis Order":"568","RAW_FILE_NAME":"CIOB_HHEAR_U1_21"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_22", "Factors":{"Sample Type":"HHEAR U1","Batch":"6"}, "Additional sample data":{"Analysis Order":"595","RAW_FILE_NAME":"CIOB_HHEAR_U1_22"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_23", "Factors":{"Sample Type":"HHEAR U1","Batch":"6"}, "Additional sample data":{"Analysis Order":"623","RAW_FILE_NAME":"CIOB_HHEAR_U1_23"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U1_24", "Factors":{"Sample Type":"HHEAR U1","Batch":"6"}, "Additional sample data":{"Analysis Order":"643","RAW_FILE_NAME":"CIOB_HHEAR_U1_24"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U2_1", "Factors":{"Sample Type":"HHEAR U2","Batch":"1"}, "Additional sample data":{"Analysis Order":"27","RAW_FILE_NAME":"CIOB_HHEAR_U2_1"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U2_2", "Factors":{"Sample Type":"HHEAR U2","Batch":"1"}, "Additional sample data":{"Analysis Order":"54","RAW_FILE_NAME":"CIOB_HHEAR_U2_2"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U2_3", "Factors":{"Sample Type":"HHEAR U2","Batch":"1"}, "Additional sample data":{"Analysis Order":"82","RAW_FILE_NAME":"CIOB_HHEAR_U2_3"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U2_4", "Factors":{"Sample Type":"HHEAR U2","Batch":"1"}, "Additional sample data":{"Analysis Order":"109","RAW_FILE_NAME":"CIOB_HHEAR_U2_4"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U2_5", "Factors":{"Sample Type":"HHEAR U2","Batch":"2"}, "Additional sample data":{"Analysis Order":"138","RAW_FILE_NAME":"CIOB_HHEAR_U2_5"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U2_6", "Factors":{"Sample Type":"HHEAR U2","Batch":"2"}, "Additional sample data":{"Analysis Order":"165","RAW_FILE_NAME":"CIOB_HHEAR_U2_6"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U2_7", "Factors":{"Sample Type":"HHEAR U2","Batch":"2"}, "Additional sample data":{"Analysis Order":"193","RAW_FILE_NAME":"CIOB_HHEAR_U2_7"} }, { "Subject ID":"-", "Sample ID":"CIOB_HHEAR_U2_8", "Factors":{"Sample Type":"HHEAR U2","Batch":"2"}, "Additional sample data":{"Analysis Order":"220","RAW_FILE_NAME":"CIOB_HHEAR_U2_8"} }, { "Subject 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ID":"CIOB_S_459", "Factors":{"Sample Type":"Study sample","Batch":"6"}, "Additional sample data":{"Analysis Order":"634","RAW_FILE_NAME":"CIOB_S_459"} }, { "Subject ID":"EM-UA5N5-U-00-13102", "Sample ID":"CIOB_S_460", "Factors":{"Sample Type":"Study sample","Batch":"6"}, "Additional sample data":{"Analysis Order":"635","RAW_FILE_NAME":"CIOB_S_460"} }, { "Subject ID":"EM-NE368-U-00-13102", "Sample ID":"CIOB_S_461", "Factors":{"Sample Type":"Study sample","Batch":"6"}, "Additional sample data":{"Analysis Order":"639","RAW_FILE_NAME":"CIOB_S_461"} }, { "Subject ID":"EM-99T30-U-00-13102", "Sample ID":"CIOB_S_462", "Factors":{"Sample Type":"Study sample","Batch":"6"}, "Additional sample data":{"Analysis Order":"640","RAW_FILE_NAME":"CIOB_S_462"} }, { "Subject ID":"EM-3NA46-U-00-13102", "Sample ID":"CIOB_S_463", "Factors":{"Sample Type":"Study sample","Batch":"6"}, "Additional sample data":{"Analysis Order":"641","RAW_FILE_NAME":"CIOB_S_463"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"N/A","SAMPLE_TYPE":"Urine","STORAGE_CONDITIONS":"-80℃"}, "TREATMENT":{"TREATMENT_SUMMARY":"N/A"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Study samples were shipped from the UCSF School of Medicine to the NC HHEAR Hub on dry ice. The NC HHEAR Hub thawed and transferred 50 µL of the study samples to a new set of tubes and used them for the analysis. An additional 10 µL was taken from the original study sample and transferred to another tube to make the total study pool for this project, and then distributed with 50 µL per aliquot, used as quality control study pools (QC study pools) throughout the whole analysis. All sample aliquots (50 µL each) and QC study pools (50 µL each) were stored at -80° C until the day of sample preparation. HHEAR Urine Pool 1 (50 µL each), HHEAR Urine Pool 2 (50 µL each), CHEAR reference urine (50 µL each) and NIST urine (SRM 3672) reference material (50 µL each) were provided by the NC HHEAR Hub. LC-MS grade water (50 µL) was used as blanks. All samples were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference urine, CHEAR reference urine, NIST reference urine, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.","PROCESSING_STORAGE_CONDITIONS":"4℃","EXTRACTION_METHOD":"Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard","EXTRACT_STORAGE":"-80℃","SAMPLE_RESUSPENSION":"Water-Methanol (95:5, v/v)","SAMPLE_SPIKING":"Tryptophan-d5 stock solution at 500 ng/mL"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Reversed phase","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)","SOLVENT_A":"100% water; 0.1% formic acid","SOLVENT_B":"100% methanol; 0.1% formic acid","FLOW_GRADIENT":"Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5","FLOW_RATE":"0.4 mL/min","COLUMN_TEMPERATURE":"50 ℃","COLUMN_PRESSURE":"6000-10000 psi","INJECTION_TEMPERATURE":"8 ℃","INTERNAL_STANDARD":"Tryptophan-d5","ANALYTICAL_TIME":"22 min","WEAK_WASH_SOLVENT_NAME":"10% methanol/90% water; 0.1% formic acid","STRONG_WASH_SOLVENT_NAME":"75% 2-Propanol/25% Water; 0.1% formic acid"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS","DETECTOR_TYPE":"Orbitrap","SOFTWARE_VERSION":"Xcalibur 4.1.31.9","ACQUISITION_DATE":"2/13/2023-3/1/2023","DATA_FORMAT":"Profile"}, "MS":{"INSTRUMENT_NAME":"Thermo Q Exactive HF-X Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing","CAPILLARY_TEMPERATURE":"320 °C","CAPILLARY_VOLTAGE":"3.5 KV","COLLISION_ENERGY":"20-45, ramp","COLLISION_GAS":"N2","FRAGMENTATION_METHOD":"CID","ION_SPRAY_VOLTAGE":"3.5kV","IONIZATION":"ES+","MASS_ACCURACY":"5 ppm","DATAFORMAT":"Profile","DESOLVATION_GAS_FLOW":"55","DESOLVATION_TEMPERATURE":"400 ℃","MS_RESULTS_FILE":"ST002757_AN004474_Results.txt UNITS:Peak area Has m/z:Yes Has RT:Yes RT units:Minutes"} }