{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002794","ANALYSIS_ID":"AN004546","VERSION":"1","CREATED_ON":"July 23, 2023, 7:52 am"}, "PROJECT":{"PROJECT_TITLE":"Metabolomics Study on Plasma and Lung Tissue of Rats Exposed to Whole Thorax Irradiation","PROJECT_TYPE":"MS quantitative analysis","PROJECT_SUMMARY":"Although some progress has been made in the study of radiation injury, there are still no effective prevention and treatment methods for severe acute radiation syndrome or sickness (ARS). Accordingly, a thorough understanding of biological characteristics associated with high-dose radiation is essential for revealing the mechanisms underlying the varied biological processes following high dose radiation and the development of novel potent radioprotective agents. Therefore, in this paper, we investigated the metabolic characteristics of plasma and lung tissue of rats exposed to whole-thorax irradiation (WTI) by liquid chromatography-mass spectrometry (LC-MS). The correlation of metabolic characteristics between plasma and lung tissue of WTI rats was studied. Parallel metabolic pathway analysis of common differential metabolites suggests that the metabolic characteristics of plasma can reflect the metabolic changes of RILI. On this basis, the potential plasma biomarkers of RILI (acylcarnitine C9:1, acylcarnitine C5:0, taurocholic acid and tauro-α-Muricholic acid) were screened, and their dose-response patterns suggest that they have the potential for early diagnosis and evaluation of RILI.","INSTITUTE":"Soochow University","LAST_NAME":"Wang","FIRST_NAME":"Chang","ADDRESS":"Suzhou, No. 199, Renai Road, Suzhou Industrial Park","EMAIL":"wangchang@suda.edu.cn","PHONE":"+86-512-65880067"}, "STUDY":{"STUDY_TITLE":"Metabolomics Study on Plasma and Lung Tissue of Rats Exposed to Whole Thorax Irradiation.","STUDY_SUMMARY":"Although some progress has been made in the study of radiation injury, there are still no effective prevention and treatment methods for severe acute radiation syndrome or sickness (ARS). Accordingly, a thorough understanding of biological characteristics associated with high-dose radiation is essential for revealing the mechanisms underlying the varied biological processes following high dose radiation and the development of novel potent radioprotective agents. In present study, plasma metabolic characteristics were investigated from patients of hematopoietic stem cell transplantation following high-dose TBI pretreatment utilizing gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). The most potential panel of four metabolic markers for radiation injury was selected and the metabolic disorders involved were explored. The metabolic disorders implied the dysregulation of gut microflora, shift of energy supply from aerobic respiration to ketogenesis, protein synthesis and metabolism in response to TBI. Although similar metabolic alternation patterns exist between male and female following high-dose irradiation, specific changes are observed in either male or female patients. These findings provide valuable information for further selecting biomarkers, clues of pathogenic mechanisms involved in high-dose radiation exposure.","INSTITUTE":"Soochow University","LAST_NAME":"Wang","FIRST_NAME":"Chang","ADDRESS":"Suzhou, No. 199, Renai Road, Suzhou Industrial Park","EMAIL":"wangchang@suda.edu.cn","PHONE":"+8651265880067"}, "SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Rattus norvegicus","GENDER":"Female"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"20210318-C1-3-5h", "Sample ID":"20210318-C1-3-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C1-3-5h.raw"} }, { "Subject ID":"20210318-C1-4-5h", "Sample ID":"20210318-C1-4-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C1-4-5h.raw"} }, { "Subject ID":"20210318-C1-6-5h", "Sample ID":"20210318-C1-6-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C1-6-5h.raw"} }, { "Subject ID":"20210318-C2-1-5h", "Sample ID":"20210318-C2-1-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C2-1-5h.raw"} }, { "Subject ID":"20210318-C3-3-5h", "Sample ID":"20210318-C3-3-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C3-3-5h.raw"} }, { "Subject ID":"20210318-C3-6-5h", "Sample ID":"20210318-C3-6-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C3-6-5h.raw"} }, { "Subject ID":"20210318-C4-6-5h", "Sample ID":"20210318-C4-6-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C4-6-5h.raw"} }, { "Subject ID":"20210318-C5-6-5h", "Sample ID":"20210318-C5-6-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C5-6-5h.raw"} }, { "Subject ID":"20210318-C6-1-5h", "Sample ID":"20210318-C6-1-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C6-1-5h.raw"} }, { "Subject ID":"20210318-C6-4-5h", "Sample ID":"20210318-C6-4-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C6-4-5h.raw"} }, { "Subject ID":"20210318-C6-6-5h", "Sample ID":"20210318-C6-6-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C6-6-5h.raw"} }, { "Subject ID":"20210318-C8-2-5h", "Sample ID":"20210318-C8-2-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C8-2-5h.raw"} }, { "Subject ID":"20210318-C8-6-5h", "Sample ID":"20210318-C8-6-5h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-C8-6-5h.raw"} }, { "Subject ID":"20210318-10Gy-1-1-5h", "Sample ID":"20210318-10Gy-1-1-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-10Gy-1-1-5h.raw"} }, { "Subject ID":"20210318-10Gy-1-2-5h", "Sample ID":"20210318-10Gy-1-2-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-10Gy-1-2-5h.raw"} }, { "Subject ID":"20210318-10Gy-2-2-5h", "Sample ID":"20210318-10Gy-2-2-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-10Gy-2-2-5h.raw"} }, { "Subject ID":"20210318-10Gy-4-3-5h", "Sample ID":"20210318-10Gy-4-3-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-10Gy-4-3-5h.raw"} }, { "Subject ID":"20210318-10Gy-5-4-5h", "Sample ID":"20210318-10Gy-5-4-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-10Gy-5-4-5h.raw"} }, { "Subject ID":"20210318-10Gy-7-3-5h", "Sample ID":"20210318-10Gy-7-3-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-10Gy-7-3-5h.raw"} }, { "Subject ID":"20210318-10Gy-8-3-5h", "Sample ID":"20210318-10Gy-8-3-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-10Gy-8-3-5h.raw"} }, { "Subject ID":"20210318-10Gy-8-4-5h", "Sample ID":"20210318-10Gy-8-4-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-10Gy-8-4-5h.raw"} }, { "Subject ID":"20210318-10Gy-9-3-5h", "Sample ID":"20210318-10Gy-9-3-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-10Gy-9-3-5h.raw"} }, { "Subject ID":"20210318-10Gy-9-5-5h", "Sample ID":"20210318-10Gy-9-5-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-10Gy-9-5-5h.raw"} }, { "Subject ID":"20210318-20Gy-1-5-5h", "Sample ID":"20210318-20Gy-1-5-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-20Gy-1-5-5h.raw"} }, { "Subject ID":"20210318-20Gy-2-4-5h", "Sample ID":"20210318-20Gy-2-4-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-20Gy-2-4-5h.raw"} }, { "Subject ID":"20210318-20Gy-3-5-5h", "Sample ID":"20210318-20Gy-3-5-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-20Gy-3-5-5h.raw"} }, { "Subject ID":"20210318-20Gy-4-2-5h", "Sample ID":"20210318-20Gy-4-2-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-20Gy-4-2-5h.raw"} }, { "Subject ID":"20210318-20Gy-5-1-5h", "Sample ID":"20210318-20Gy-5-1-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-20Gy-5-1-5h.raw"} }, { "Subject ID":"20210318-20Gy-5-3-5h", "Sample ID":"20210318-20Gy-5-3-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-20Gy-5-3-5h.raw"} }, { "Subject ID":"20210318-20Gy-6-2-5h", "Sample ID":"20210318-20Gy-6-2-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-20Gy-6-2-5h.raw"} }, { "Subject ID":"20210318-20Gy-6-3-5h", "Sample ID":"20210318-20Gy-6-3-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-20Gy-6-3-5h.raw"} }, { "Subject ID":"20210318-20Gy-7-1-5h", "Sample ID":"20210318-20Gy-7-1-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-20Gy-7-1-5h.raw"} }, { "Subject ID":"20210318-20Gy-8-5-5h", "Sample ID":"20210318-20Gy-8-5-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-20Gy-8-5-5h.raw"} }, { "Subject ID":"20210318-20Gy-9-4-5h", "Sample ID":"20210318-20Gy-9-4-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-20Gy-9-4-5h.raw"} }, { "Subject ID":"20210318-20Gy-9-6-5h", "Sample ID":"20210318-20Gy-9-6-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-20Gy-9-6-5h.raw"} }, { "Subject ID":"20210318-35Gy-2-5-5h", "Sample ID":"20210318-35Gy-2-5-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-35Gy-2-5-5h.raw"} }, { "Subject ID":"20210318-35Gy-2-6-5h", "Sample ID":"20210318-35Gy-2-6-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-35Gy-2-6-5h.raw"} }, { "Subject ID":"20210318-35Gy-3-1-5h", "Sample ID":"20210318-35Gy-3-1-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-35Gy-3-1-5h.raw"} }, { "Subject ID":"20210318-35Gy-3-2-5h", "Sample ID":"20210318-35Gy-3-2-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-35Gy-3-2-5h.raw"} }, { "Subject ID":"20210318-35Gy-3-4-5h", "Sample ID":"20210318-35Gy-3-4-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-35Gy-3-4-5h.raw"} }, { "Subject ID":"20210318-35Gy-4-4-5h", "Sample ID":"20210318-35Gy-4-4-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-35Gy-4-4-5h.raw"} }, { "Subject ID":"20210318-35Gy-4-5-5h", "Sample ID":"20210318-35Gy-4-5-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-35Gy-4-5-5h.raw"} }, { "Subject ID":"20210318-35Gy-5-2-5h", "Sample ID":"20210318-35Gy-5-2-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-35Gy-5-2-5h.raw"} }, { "Subject ID":"20210318-35Gy-7-2-5h", "Sample ID":"20210318-35Gy-7-2-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-35Gy-7-2-5h.raw"} }, { "Subject ID":"20210318-35Gy-7-5-5h", "Sample ID":"20210318-35Gy-7-5-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-35Gy-7-5-5h.raw"} }, { "Subject ID":"20210318-35Gy-9-2-5h", "Sample ID":"20210318-35Gy-9-2-5h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210318-35Gy-9-2-5h.raw"} }, { "Subject ID":"20210329-C1-3-24h", "Sample ID":"20210329-C1-3-24h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-C1-3-24h.raw"} }, { "Subject ID":"20210329-C1-6-24h", "Sample ID":"20210329-C1-6-24h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-C1-6-24h.raw"} }, { "Subject ID":"20210329-C2-1-24h", "Sample ID":"20210329-C2-1-24h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-C2-1-24h.raw"} }, { "Subject ID":"20210329-C3-3-24h", "Sample ID":"20210329-C3-3-24h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-C3-3-24h.raw"} }, { "Subject ID":"20210329-C3-6-24h", "Sample ID":"20210329-C3-6-24h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-C3-6-24h.raw"} }, { "Subject ID":"20210329-C4-6-24h", "Sample ID":"20210329-C4-6-24h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-C4-6-24h.raw"} }, { "Subject ID":"20210329-C5-6-24h", "Sample ID":"20210329-C5-6-24h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-C5-6-24h.raw"} }, { "Subject ID":"20210329-C6-1-24h", "Sample ID":"20210329-C6-1-24h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-C6-1-24h.raw"} }, { "Subject ID":"20210329-C6-4-24h", "Sample ID":"20210329-C6-4-24h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-C6-4-24h.raw"} }, { "Subject ID":"20210329-C6-6-24h", "Sample ID":"20210329-C6-6-24h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-C6-6-24h.raw"} }, { "Subject ID":"20210329-C8-2-24h", "Sample ID":"20210329-C8-2-24h", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-C8-2-24h.raw"} }, { "Subject ID":"20210329-10Gy-1-1-24h", "Sample ID":"20210329-10Gy-1-1-24h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-10Gy-1-1-24h.raw"} }, { "Subject ID":"20210329-10Gy-1-2-24h", "Sample ID":"20210329-10Gy-1-2-24h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-10Gy-1-2-24h.raw"} }, { "Subject ID":"20210329-10Gy-2-2-24h", "Sample ID":"20210329-10Gy-2-2-24h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-10Gy-2-2-24h.raw"} }, { "Subject ID":"20210329-10Gy-4-3-24h", "Sample ID":"20210329-10Gy-4-3-24h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-10Gy-4-3-24h.raw"} }, { "Subject ID":"20210329-10Gy-5-4-24h", "Sample ID":"20210329-10Gy-5-4-24h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-10Gy-5-4-24h.raw"} }, { "Subject ID":"20210329-10Gy-7-3-24h", "Sample ID":"20210329-10Gy-7-3-24h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-10Gy-7-3-24h.raw"} }, { "Subject ID":"20210329-10Gy-8-3-24h", "Sample ID":"20210329-10Gy-8-3-24h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-10Gy-8-3-24h.raw"} }, { "Subject ID":"20210329-10Gy-8-4-24h", "Sample ID":"20210329-10Gy-8-4-24h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-10Gy-8-4-24h.raw"} }, { "Subject ID":"20210329-10Gy-9-3-24h", "Sample ID":"20210329-10Gy-9-3-24h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-10Gy-9-3-24h.raw"} }, { "Subject ID":"20210329-10Gy-9-5-24h", "Sample ID":"20210329-10Gy-9-5-24h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-10Gy-9-5-24h.raw"} }, { "Subject ID":"20210329-20Gy-1-5-24h", "Sample ID":"20210329-20Gy-1-5-24h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-20Gy-1-5-24h.raw"} }, { "Subject ID":"20210329-20Gy-2-4-24h", "Sample ID":"20210329-20Gy-2-4-24h", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"1a","RAW_FILE_NAME":"GYF-plasma-neg-GYF_plasma-neg_20210329-20Gy-2-4-24h.raw"} }, { "Subject ID":"20210329-20Gy-3-5-24h", "Sample ID":"20210329-20Gy-3-5-24h", "Factors":{"Treatment":"WTI"}, "Additional sample 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"Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-20Gy-9-4.raw"} }, { "Subject ID":"20210527-20Gy-9-6", "Sample ID":"20210527-20Gy-9-6", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-20Gy-9-6.raw"} }, { "Subject ID":"20210527-35Gy-2-5", "Sample ID":"20210527-35Gy-2-5", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-35Gy-2-5.raw"} }, { "Subject ID":"20210527-35Gy-2-6", "Sample ID":"20210527-35Gy-2-6", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-35Gy-2-6.raw"} }, { "Subject ID":"20210527-35Gy-3-1", "Sample ID":"20210527-35Gy-3-1", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-35Gy-3-1.raw"} }, { "Subject ID":"20210527-35Gy-3-4", "Sample ID":"20210527-35Gy-3-4", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-35Gy-3-4.raw"} }, { "Subject ID":"20210527-35Gy-4-4", "Sample ID":"20210527-35Gy-4-4", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-35Gy-4-4.raw"} }, { "Subject ID":"20210527-35Gy-5-2", "Sample ID":"20210527-35Gy-5-2", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-35Gy-5-2.raw"} }, { "Subject ID":"20210527-35Gy-7-2", "Sample ID":"20210527-35Gy-7-2", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-35Gy-7-2.raw"} }, { "Subject ID":"20210527-35Gy-7-5", "Sample ID":"20210527-35Gy-7-5", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-35Gy-7-5.raw"} }, { "Subject ID":"20210527-35Gy-9-2", "Sample ID":"20210527-35Gy-9-2", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-35Gy-9-2.raw"} }, { "Subject ID":"20210527-C1-6", "Sample ID":"20210527-C1-6", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-C1-6.raw"} }, { "Subject ID":"20210527-C2-1", "Sample ID":"20210527-C2-1", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-C2-1.raw"} }, { "Subject ID":"20210527-C3-3", "Sample ID":"20210527-C3-3", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-C3-3.raw"} }, { "Subject ID":"20210527-C3-6", "Sample ID":"20210527-C3-6", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-C3-6.raw"} }, { "Subject ID":"20210527-C4-6", "Sample ID":"20210527-C4-6", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-C4-6.raw"} }, { "Subject ID":"20210527-C5-6", "Sample ID":"20210527-C5-6", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-C5-6.raw"} }, { "Subject ID":"20210527-C6-1", "Sample ID":"20210527-C6-1", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-C6-1.raw"} }, { "Subject ID":"20210527-C6-4", "Sample ID":"20210527-C6-4", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-C6-4.raw"} }, { "Subject ID":"20210527-C6-6", "Sample ID":"20210527-C6-6", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-C6-6.raw"} }, { "Subject ID":"20210527-C8-2", "Sample ID":"20210527-C8-2", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-C8-2.raw"} }, { "Subject ID":"20210527-C8-6", "Sample ID":"20210527-C8-6", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2a","RAW_FILE_NAME":"GYF_lung-pos_20210527-C8-6.raw"} }, { "Subject ID":"20210530-10Gy-1-1", "Sample ID":"20210530-10Gy-1-1", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-10Gy-1-1.raw"} }, { "Subject ID":"20210530-10Gy-1-2", "Sample ID":"20210530-10Gy-1-2", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-10Gy-1-2.raw"} }, { "Subject ID":"20210530-10Gy-2-2", "Sample ID":"20210530-10Gy-2-2", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-10Gy-2-2.raw"} }, { "Subject ID":"20210530-10Gy-4-3", "Sample ID":"20210530-10Gy-4-3", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-10Gy-4-3.raw"} }, { "Subject ID":"20210530-10Gy-5-4", "Sample ID":"20210530-10Gy-5-4", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-10Gy-5-4.raw"} }, { "Subject ID":"20210530-10Gy-7-3", "Sample ID":"20210530-10Gy-7-3", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-10Gy-7-3.raw"} }, { "Subject ID":"20210530-10Gy-8-3", "Sample ID":"20210530-10Gy-8-3", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-10Gy-8-3.raw"} }, { "Subject ID":"20210530-10Gy-8-4", "Sample ID":"20210530-10Gy-8-4", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-10Gy-8-4.raw"} }, { "Subject ID":"20210530-10Gy-9-3", "Sample ID":"20210530-10Gy-9-3", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-10Gy-9-3.raw"} }, { "Subject ID":"20210530-10Gy-9-5", "Sample ID":"20210530-10Gy-9-5", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-10Gy-9-5.raw"} }, { "Subject ID":"20210530-20Gy-2-4", "Sample ID":"20210530-20Gy-2-4", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-20Gy-2-4.raw"} }, { "Subject ID":"20210530-20Gy-3-5", "Sample ID":"20210530-20Gy-3-5", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-20Gy-3-5.raw"} }, { "Subject ID":"20210530-20Gy-4-2", "Sample ID":"20210530-20Gy-4-2", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-20Gy-4-2.raw"} }, { "Subject ID":"20210530-20Gy-5-1", "Sample ID":"20210530-20Gy-5-1", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-20Gy-5-1.raw"} }, { "Subject ID":"20210530-20Gy-6-3", "Sample ID":"20210530-20Gy-6-3", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-20Gy-6-3.raw"} }, { "Subject ID":"20210530-20Gy-7-1", "Sample ID":"20210530-20Gy-7-1", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-20Gy-7-1.raw"} }, { "Subject ID":"20210530-20Gy-8-5", "Sample ID":"20210530-20Gy-8-5", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-20Gy-8-5.raw"} }, { "Subject ID":"20210530-20Gy-9-4", "Sample ID":"20210530-20Gy-9-4", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-20Gy-9-4.raw"} }, { "Subject ID":"20210530-20Gy-9-6", "Sample ID":"20210530-20Gy-9-6", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-20Gy-9-6.raw"} }, { "Subject ID":"20210530-35Gy-2-5", "Sample ID":"20210530-35Gy-2-5", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-35Gy-2-5.raw"} }, { "Subject ID":"20210530-35Gy-2-6", "Sample ID":"20210530-35Gy-2-6", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-35Gy-2-6.raw"} }, { "Subject ID":"20210530-35Gy-3-1", "Sample ID":"20210530-35Gy-3-1", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-35Gy-3-1.raw"} }, { "Subject ID":"20210530-35Gy-3-4", "Sample ID":"20210530-35Gy-3-4", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-35Gy-3-4.raw"} }, { "Subject ID":"20210530-35Gy-4-4", "Sample ID":"20210530-35Gy-4-4", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-35Gy-4-4.raw"} }, { "Subject ID":"20210530-35Gy-5-2", "Sample ID":"20210530-35Gy-5-2", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-35Gy-5-2.raw"} }, { "Subject ID":"20210530-35Gy-7-2", "Sample ID":"20210530-35Gy-7-2", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-35Gy-7-2.raw"} }, { "Subject ID":"20210530-35Gy-7-5", "Sample ID":"20210530-35Gy-7-5", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-35Gy-7-5.raw"} }, { "Subject ID":"20210530-35Gy-9-2", "Sample ID":"20210530-35Gy-9-2", "Factors":{"Treatment":"WTI"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-35Gy-9-2.raw"} }, { "Subject ID":"20210530-C1-6", "Sample ID":"20210530-C1-6", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-C1-6.raw"} }, { "Subject ID":"20210530-C2-1", "Sample ID":"20210530-C2-1", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-C2-1.raw"} }, { "Subject ID":"20210530-C3-3", "Sample ID":"20210530-C3-3", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-C3-3.raw"} }, { "Subject ID":"20210530-C3-6", "Sample ID":"20210530-C3-6", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-C3-6.raw"} }, { "Subject ID":"20210530-C4-6", "Sample ID":"20210530-C4-6", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-C4-6.raw"} }, { "Subject ID":"20210530-C5-6", "Sample ID":"20210530-C5-6", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-C5-6.raw"} }, { "Subject ID":"20210530-C6-1", "Sample ID":"20210530-C6-1", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-C6-1.raw"} }, { "Subject ID":"20210530-C6-4", "Sample ID":"20210530-C6-4", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-C6-4.raw"} }, { "Subject ID":"20210530-C6-6", "Sample ID":"20210530-C6-6", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-C6-6.raw"} }, { "Subject ID":"20210530-C8-2", "Sample ID":"20210530-C8-2", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-C8-2.raw"} }, { "Subject ID":"20210530-C8-6", "Sample ID":"20210530-C8-6", "Factors":{"Treatment":"Control"}, "Additional sample data":{"Batch":"2b","RAW_FILE_NAME":"GYF_lung-neg_20210530-C8-6.raw"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Whole blood was collected about 5 hours, 1 day, 2 days, 3 days, and 5 days before and after irradiation, and tissue samples were taken on the fifth day。","SAMPLE_TYPE":"Blood (whole)"}, "TREATMENT":{"TREATMENT_SUMMARY":"After anesthesia with 10% chloral hydrate solution (0.25 ml/100 g) by intraperitoneal injection, the small animal radiation therapy simulator (X-RAD SmART) was used to irradiate the whole lung tissue using a 40×40 mm lead collimator. Whole chest X-ray irradiation of 10 Gy, 20 Gy and 35 Gy in each dose group was given by anterior-anterior-field cross-irradiation, and the dose rate was about 2.7 Gy/min. The control group was also anesthetized, but not irradiated"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Plasma:Add 200 μL ice acetonitrile containing internal standard (internal standard and its concentration are shown in Table 1-3) to 50 μL plasma, swirl and mix well for 2 min, centrifuge at 13,000 rpm and 4 ℃ for 15 min, and divide the supernatant into two parts in parallel (100 μL each for determination and analysis of positive and negative ion modes of LC-MS). Freeze-dried and stored in a -80 ℃ refrigerator. Before metabolomics analysis, the samples were redissolved with 50 μL 25% acetonitrile/water (v/v) solution。 Tissue:Weigh about 20 mg lung tissue sample powder into 2 mL EP tube, add 1.5 mL 80% methanol/water (v/v) solution containing internal standard (internal standard and its concentration are shown in Table 2-1), swirl and mix well for 2 min, then add 3 zirconium beads into the sample and grind at 45 hz for 2 times, 5 min each time. The supernatant was divided into two parts in parallel (600 μL each for determination of positive and negative ion modes of LC-MS) at an interval of 15 s, and then centrifuged at 13,000 rpm for 15 min at 4 ℃. The supernatant was freeze-dried and stored in a refrigerator at -80 ℃. Before metabolomics analysis, samples were redissolved with 100 μL 80% methanol/water (v/v) solution."}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"positive ion mode","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo TSQ Vantage HPLC-MS/MS","COLUMN_NAME":"Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um)","SOLVENT_A":"water(0.1% formic acid )","SOLVENT_B":"acetonitrile(0.1% formic acid )","FLOW_GRADIENT":"0~0.5min, 5% B; 0.5 ~24min, 5~100% B; 24-28 min, 40-100% B; 28~28.5min, 100~5% B; 28.5~48 min, 5% B","FLOW_RATE":"0.35 mL/min","COLUMN_TEMPERATURE":"50"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"INSTRUMENT_NAME":"Thermo TSQ Vantage","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"The temperature of ion source and ion transport tube is 350 ℃, the electrospray voltage in positive and negative ion mode is 3,000 V respectively, the sheath pressure is 35 Arb, and the auxiliary pressure is 10 Arb. Data acquisition and pre-processing and instrument interface control are performed in Xcalibur (Thermo, USA) software."}, "MS_METABOLITE_DATA":{ "Units":"peak area", 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} }