{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002847","ANALYSIS_ID":"AN004665","VERSION":"1","CREATED_ON":"September 6, 2023, 5:45 pm"},

"PROJECT":{"PROJECT_TITLE":"Targeting Pancreatic Cancer Metabolic Dependencies through Glutamine Antagonism.","PROJECT_TYPE":"Manuscript","PROJECT_SUMMARY":"Pancreatic ductal adenocarcinoma (PDAC) cells utilize glutamine (Gln) to support proliferation and redox balance. Earlier attempts to inhibit Gln metabolism using glutaminase inhibitors resulted in rapid metabolic reprogramming and therapeutic resistance. We demonstrated that treating PDAC cells with a Gln antagonist, 6-Diazo-5-oxo-L-norleucine (DON), led to a metabolic crisis in vitro. In addition, we observed a profound decrease in tumor growth in various in vivo models using DRP-104 (sirpiglenastat), a pro-drug version of DON designed to circumvent DON-associated toxicity.","INSTITUTE":"New York University","DEPARTMENT":"Radiation Oncology","LABORATORY":"Alec C Kimmelman","LAST_NAME":"Encarnacion Rosado","FIRST_NAME":"Joel","ADDRESS":"Smilow Research Building Room 907G New York, NY 10016","EMAIL":"joel.encarnacion-rosado@nyulangone.org","PHONE":"646-501-8984"},

"STUDY":{"STUDY_TITLE":"Targeting Pancreatic Cancer Metabolic Dependencies through Glutamine Antagonism.","STUDY_SUMMARY":"Pancreatic ductal adenocarcinoma (PDAC) cells utilize glutamine (Gln) to support proliferation and redox balance. Earlier attempts to inhibit Gln metabolism using glutaminase inhibitors resulted in rapid metabolic reprogramming and therapeutic resistance. Here, we demonstrated that treating PDAC cells with a Gln antagonist, 6-Diazo-5-oxo-L-norleucine (DON), led to a metabolic crisis in vitro. In addition, we observed a profound decrease in tumor growth in various in vivo models using DRP-104 (sirpiglenastat), a pro-drug version of DON that was designed to circumvent DON associated toxicity. We found that ERK signaling is increased as a compensatory mechanism. Combinatorial treatment of DRP-104 and Trametinib led to a significant increase in survival in a syngeneic model PDAC. These proof-of-concept studies suggested that broadly targeting Gln metabolism could provide a therapeutic avenue for PDAC. The combination with an ERK signaling pathway inhibitor could further improve the therapeutic outcome.","INSTITUTE":"New York University","LAST_NAME":"Encarnacion Rosado","FIRST_NAME":"Joel","ADDRESS":"Smilow Research Building Room 907G New York, NY 10016","EMAIL":"joel.encarnacion-rosado@nyulangone.org","PHONE":"646-501-8984"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Not applicable"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"HY19_CNT_1",
"Factors":{"Vehicle":"Vehicle"},
"Additional sample data":{"RAW_FILE_NAME":"S23446.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"HY19_CNT_2",
"Factors":{"Vehicle":"Vehicle"},
"Additional sample data":{"RAW_FILE_NAME":"S23447.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"HY19_CNT_3",
"Factors":{"Vehicle":"Vehicle"},
"Additional sample data":{"RAW_FILE_NAME":"S23448.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"HY19_DON_1",
"Factors":{"Vehicle":"DON"},
"Additional sample data":{"RAW_FILE_NAME":"S23449.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"HY19_DON_2",
"Factors":{"Vehicle":"DON"},
"Additional sample data":{"RAW_FILE_NAME":"S23450.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"HY19_DON_3",
"Factors":{"Vehicle":"DON"},
"Additional sample data":{"RAW_FILE_NAME":"S23451.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"HY19_DRP104_1",
"Factors":{"Vehicle":"DRP-104"},
"Additional sample data":{"RAW_FILE_NAME":"S23452.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"HY19_DRP104_2",
"Factors":{"Vehicle":"DRP-104"},
"Additional sample data":{"RAW_FILE_NAME":"S23453.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"HY19_DRP104_3",
"Factors":{"Vehicle":"DRP-104"},
"Additional sample data":{"RAW_FILE_NAME":"S23454.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"As described in Parker, et al (https://doi.org/10.1038/s41467-021-25228-9), xtraction of metabolites from cell pellets— Metabolites were initially extracted from samples by quickly aspirating the cell culture media and adding 1 mL of extraction buffer, consisting of 80% methanol (Fisher Scientific) and 500 nM metabolomics amino acid mix standard (Cambridge Isotope Laboratories). To effectively scale all harvested samples to equivalent volumes of extraction buffer, samples were fully dried down by Speedvac (Thermo Fisher, Waltham, MA) and reconstituted volumetrically by mixing the entire dried cell pellet sample with 1 mL of 80% methanol without QC standards in 2.0 mL screw cap vials containing ~100 µL of disruption beads (Research Products International, Mount Prospect, IL). Samples were scaled to a ratio of 1e6 cells to 1 mL of extraction solvent with all steps being carried out in a cold room. Each was homogenized for 10 cycles on a bead blaster homogenizer (Benchmark Scientific, Edison, NJ). Cycling consisted of a 30 sec homogenization time at 6 m/s followed by a 30 sec pause. Samples were subsequently spun at 21,000 × g for 3 min at 4 °C. A set volume of each (450 µL) was transferred to a 1.5 mL tube and dried down by Speedvac concentration. Samples were reconstituted in 50 µL of Optima LC/MS grade water (Fisher Scientific, Waltham, MA). Samples were sonicated for 2 min, then centrifuged at 21,000 × g for 3 min at 4 °C. Twenty microliters were transferred to LC vials containing glass inserts for analysis. The remaining sample was placed at −80 °C for long-term storage. Samples were subjected to an LC-MS analysis to detect and quantify known peaks. A metabolite extraction was carried out on each sample by quickly aspirating experimental media and adding 1 mL of 80% methanol containing internal QC standards. A MilliporeTM ZIC-pHILIC (2.1 × 150 mm, 5 μm) LC column was coupled to a Dionex Ultimate 3000TM system. The column oven temperature and flow rate were set to 25 °C and 100 μL/min, respectively, for the following gradient elution: 80–20%B (0–30 min), 20–80%B (30–31 min), 80–80%B (31–42 min). Mobile phase compositions were: (A) 10 mM ammonium carbonate in water, pH 9.0 and (B) neat acetonitrile; and an injection volume of 2 μL was used for all analyses. The LC system was coupled to a Thermo Q Exactive HFTM mass spectrometer operating in heated electrospray ionization mode (HESI) for LC-MS analysis. A 30-m polarity switching data-dependent Top 5 method was used for both positive and negative modes. The following parameters were also set: spray voltage of 3.5 kV, capillary temperature at 320 °C, sheath gas flow rate of 35, aux gas rate of 10, and max spray current of 100 μA. Full MS scan parameters for both positive and negative modes were set as followed: scan range of 67–1000 m/z, resolution of 120,000, AGC target of 3e6, and maximum IT of 100 ms. Tandem MS spectra for both positive and negative modes used a resolution of 15,000, fixed first mass of 50 m/z, isolation window of 0.4 m/z, isolation offset of 0.1 m/z, AGC target of 1e5, minimum AGC target of 1e4, intensity threshold of 2e5, maximum IT of 50 ms, and three-way multiplexed normalized collision energies (nCE) of 10, 30, 80. All data were acquired in profile mode. ThermoTM RAW files were read using ThermoFisher CommonCore RawFileReader. An in-house python script (Skeleton) was used for detection and quantification of sample peaks and internal standards based on a retention time and accurate mass library adapted from the Whitehead Institute94 and verified with authentic standards and/or high-resolution MS/MS spectra manually curated using the NIST14MS/MS95 and METLIN (2017)96 tandem mass spectral libraries. For feature-based analysis, an in-house python script (Ungrid) was used to detect MS1 peaks across all samples using the following parameters: a m/z discrimination threshold of 20 ppm, a minimum peak intensity of 1e5, a minimum signal-to-noise ratio of 10, and a retention time threshold of 2 min. Metabolite and feature peaks extracted in this manner were defined by either the detected feature m/z or the theoretical m/z of the expected ion type for the standard in the library (e.g., [M+H]+). The following parameters were applied: a ±5 part-per-million (ppm) tolerance, an initial retention time search window of ±0.5 min across all samples, and a ±7.5 s peak apex retention time tolerance within individual samples. An in-house statistical pipeline, Metabolize (version 1.0), was used to process the resulting data matrix of metabolite intensities for all samples and blank controls. A final peak detection was calculated based on a signal-to-noise ratio (S/N) or 3× blank controls with a floor of 1e5 (arb. units). The threshold value was input for any sample where the calculated peak intensity was lower than the blank threshold for any statistical comparisons. The resulting blank corrected data matrix was used for all group-wise comparisons. T-tests were performed using the Python SciPy library (version 1.1.0)97 to test for differences and generate statistics. Any metabolite with p-value < 0.05 was considered significantly regulated (up or down). Volcano plots were generated utilizing Prism (GraphPad). The R package DESeq2 (1.24.0)98 was used to adjust for covariate effects (as applicable) and to calculate the adjusted p-value in the covariate model. Zero values were input for non-detected values instead of the blank threshold to avoid false positive.","SAMPLE_TYPE":"Cultured cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"HY19636 cells were plated in a six-well plate at 2.0x10^5 cells/well and allowed to attach overnight in DMEM. Next, cells were washed with PBS twice and cultured for 24 hours in DMEM supplemented with 10% dialyzed serum was added. Cells were pre-treated with DON (25µM) or DRP-104 (25µM) overnight, media was removed and washed with PBS. Then, cells were frozen in -80C and until metabolite extraction"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Extraction of metabolites from cell pellets— Metabolites were initially extracted from samples by quickly aspirating the cell culture media and adding 1 mL of extraction buffer, consisting of 80% methanol (Fisher Scientific) and 500 nM metabolomics amino acid mix standard (Cambridge Isotope Laboratories). To effectively scale all harvested samples to equivalent volumes of extraction buffer, samples were fully dried down by Speedvac (Thermo Fisher, Waltham, MA) and reconstituted volumetrically by mixing the entire dried cell pellet sample with 1 mL of 80% methanol without QC standards in 2.0 mL screw cap vials containing ~100 µL of disruption beads (Research Products International, Mount Prospect, IL). Samples were scaled to a ratio of 1e6 cells to 1 mL of extraction solvent with all steps being carried out in a cold room. Each was homogenized for 10 cycles on a bead blaster homogenizer (Benchmark Scientific, Edison, NJ). Cycling consisted of a 30 sec homogenization time at 6 m/s followed by a 30 sec pause. Samples were subsequently spun at 21,000 × g for 3 min at 4 °C. A set volume of each (450 µL) was transferred to a 1.5 mL tube and dried down by Speedvac concentration. Samples were reconstituted in 50 µL of Optima LC/MS grade water (Fisher Scientific, Waltham, MA). Samples were sonicated for 2 min, then centrifuged at 21,000 × g for 3 min at 4 °C. Twenty microliters were transferred to LC vials containing glass inserts for analysis. The remaining sample was placed at −80 °C for long-term storage."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"SeQuant ZIC- pHILIC (150 x 2.1mm, 5um)","SOLVENT_A":"10 mM ammonium carbonate in water, pH 9.0","SOLVENT_B":"acetonitrile","FLOW_GRADIENT":"The gradient profile was as follows; 80-20%B (0-30 min), 20-80%B (30-31 min), 80-80%B (31-42 min)","FLOW_RATE":"100 uL/min","COLUMN_TEMPERATURE":"25"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive HF hybrid Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"The LC system was coupled to a Thermo Q Exactive HFTM mass spectrometer operating in heated electrospray ionization mode (HESI) for LC-MS analysis. A 30-m polarity switching data-dependent Top 5 method was used for both positive and negative modes. The following parameters were also set: spray voltage of 3.5 kV, capillary temperature at 320 °C, sheath gas flow rate of 35, aux gas rate of 10, and max spray current of 100 μA. Full MS scan parameters for both positive and negative modes were set as followed: scan range of 67–1000 m/z, resolution of 120,000, AGC target of 3e6, and maximum IT of 100 ms. Tandem MS spectra for both positive and negative modes used a resolution of 15,000, fixed first mass of 50 m/z, isolation window of 0.4 m/z, isolation offset of 0.1 m/z, AGC target of 1e5, minimum AGC target of 1e4, intensity threshold of 2e5, maximum IT of 50 ms, and three-way multiplexed normalized collision energies (nCE) of 10, 30, 80. All data were acquired in profile mode. ThermoTM RAW files were read using ThermoFisher CommonCore RawFileReader. An in-house python script (Skeleton) was used for detection and quantification of sample peaks and internal standards based on a retention time and accurate mass library adapted from the Whitehead Institute94 and verified with authentic standards and/or high-resolution MS/MS spectra manually curated using the NIST14MS/MS95 and METLIN (2017)96 tandem mass spectral libraries."},

"MS_METABOLITE_DATA":{
"Units":"ion counts",

"Data":[{"Metabolite":"1-Methylnicotinamide","HY19_CNT_1":"-","HY19_CNT_2":"-","HY19_CNT_3":"-","HY19_DON_1":"82956","HY19_DON_2":"59250","HY19_DON_3":"52213","HY19_DRP104_1":"-","HY19_DRP104_2":"63795","HY19_DRP104_3":"-"},{"Metabolite":"2,3-Diphosphoglyceric acid","HY19_CNT_1":"-","HY19_CNT_2":"-","HY19_CNT_3":"-","HY19_DON_1":"-","HY19_DON_2":"-","HY19_DON_3":"-","HY19_DRP104_1":"-","HY19_DRP104_2":"-","HY19_DRP104_3":"-"},{"Metabolite":"3-Hydroxybutyric acid","HY19_CNT_1":"-","HY19_CNT_2":"-","HY19_CNT_3":"-","HY19_DON_1":"-","HY19_DON_2":"-","HY19_DON_3":"-","HY19_DRP104_1":"-","HY19_DRP104_2":"-","HY19_DRP104_3":"-"},{"Metabolite":"3-Phosphoglyceric acid","HY19_CNT_1":"6498588","HY19_CNT_2":"5386352","HY19_CNT_3":"3090533","HY19_DON_1":"2360461","HY19_DON_2":"3853256","HY19_DON_3":"2724835","HY19_DRP104_1":"1706643","HY19_DRP104_2":"3380644","HY19_DRP104_3":"2005825"},{"Metabolite":"4,5-Dihydroorotic acid","HY19_CNT_1":"-","HY19_CNT_2":"-","HY19_CNT_3":"-","HY19_DON_1":"-","HY19_DON_2":"-","HY19_DON_3":"-","HY19_DRP104_1":"-","HY19_DRP104_2":"-","HY19_DRP104_3":"-"},{"Metabolite":"4-Hydroxyproline","HY19_CNT_1":"229745","HY19_CNT_2":"130776","HY19_CNT_3":"150598","HY19_DON_1":"107326","HY19_DON_2":"133595","HY19_DON_3":"-","HY19_DRP104_1":"204345","HY19_DRP104_2":"151346","HY19_DRP104_3":"187137"},{"Metabolite":"5''-Methylthioadenosine","HY19_CNT_1":"94689","HY19_CNT_2":"96149","HY19_CNT_3":"-","HY19_DON_1":"146934","HY19_DON_2":"151846","HY19_DON_3":"144796","HY19_DRP104_1":"138294","HY19_DRP104_2":"-","HY19_DRP104_3":"124009"},{"Metabolite":"5-Phosphoribosylamine","HY19_CNT_1":"21991","HY19_CNT_2":"25390","HY19_CNT_3":"23580","HY19_DON_1":"17891","HY19_DON_2":"15094","HY19_DON_3":"16775","HY19_DRP104_1":"12341","HY19_DRP104_2":"11340","HY19_DRP104_3":"17522"},{"Metabolite":"6-Phosphogluconic acid","HY19_CNT_1":"19220","HY19_CNT_2":"27929","HY19_CNT_3":"-","HY19_DON_1":"-","HY19_DON_2":"22139","HY19_DON_3":"-","HY19_DRP104_1":"-","HY19_DRP104_2":"-","HY19_DRP104_3":"-"},{"Metabolite":"Acetoacetic acid","HY19_CNT_1":"-","HY19_CNT_2":"-","HY19_CNT_3":"-","HY19_DON_1":"-","HY19_DON_2":"-","HY19_DON_3":"41463","HY19_DRP104_1":"-","HY19_DRP104_2":"-","HY19_DRP104_3":"-"},{"Metabolite":"Acetyl-CoA","HY19_CNT_1":"-","HY19_CNT_2":"-","HY19_CNT_3":"-","HY19_DON_1":"-","HY19_DON_2":"-","HY19_DON_3":"-","HY19_DRP104_1":"-","HY19_DRP104_2":"-","HY19_DRP104_3":"-"},{"Metabolite":"Acetylcysteine","HY19_CNT_1":"17623","HY19_CNT_2":"14376","HY19_CNT_3":"-","HY19_DON_1":"34667","HY19_DON_2":"21384","HY19_DON_3":"17945","HY19_DRP104_1":"28871","HY19_DRP104_2":"15669","HY19_DRP104_3":"27542"},{"Metabolite":"Adenine","HY19_CNT_1":"18406650","HY19_CNT_2":"28721482","HY19_CNT_3":"26043286","HY19_DON_1":"16496442","HY19_DON_2":"15120138","HY19_DON_3":"14856874","HY19_DRP104_1":"489019","HY19_DRP104_2":"-","HY19_DRP104_3":"614821"},{"Metabolite":"Adenosine","HY19_CNT_1":"-","HY19_CNT_2":"1511104","HY19_CNT_3":"5815696","HY19_DON_1":"28081984","HY19_DON_2":"4786747","HY19_DON_3":"8067276","HY19_DRP104_1":"4901472","HY19_DRP104_2":"2537381","HY19_DRP104_3":"5493814"},{"Metabolite":"Adenosine diphosphate ribose","HY19_CNT_1":"1742062","HY19_CNT_2":"3768828","HY19_CNT_3":"2591039","HY19_DON_1":"2060066","HY19_DON_2":"3331700","HY19_DON_3":"2528644","HY19_DRP104_1":"688404","HY19_DRP104_2":"1070490","HY19_DRP104_3":"771052"},{"Metabolite":"Adenosine monophosphate","HY19_CNT_1":"-","HY19_CNT_2":"2160267","HY19_CNT_3":"1395076","HY19_DON_1":"2066384","HY19_DON_2":"21185746","HY19_DON_3":"13023259","HY19_DRP104_1":"2614458","HY19_DRP104_2":"10968023","HY19_DRP104_3":"-"},{"Metabolite":"Adenosine triphosphate","HY19_CNT_1":"142307","HY19_CNT_2":"143008","HY19_CNT_3":"58647","HY19_DON_1":"77767","HY19_DON_2":"118322","HY19_DON_3":"79953","HY19_DRP104_1":"-","HY19_DRP104_2":"64779","HY19_DRP104_3":"-"},{"Metabolite":"Adenylsuccinic acid","HY19_CNT_1":"-","HY19_CNT_2":"19224","HY19_CNT_3":"-","HY19_DON_1":"18303","HY19_DON_2":"25836","HY19_DON_3":"25276","HY19_DRP104_1":"-","HY19_DRP104_2":"17501","HY19_DRP104_3":"-"},{"Metabolite":"ADP","HY19_CNT_1":"489327","HY19_CNT_2":"777981","HY19_CNT_3":"447184","HY19_DON_1":"774099","HY19_DON_2":"2036000","HY19_DON_3":"1529042","HY19_DRP104_1":"357555","HY19_DRP104_2":"953806","HY19_DRP104_3":"-"},{"Metabolite":"Allantoin","HY19_CNT_1":"-","HY19_CNT_2":"-","HY19_CNT_3":"-","HY19_DON_1":"-","HY19_DON_2":"21116","HY19_DON_3":"-","HY19_DRP104_1":"-","HY19_DRP104_2":"10120","HY19_DRP104_3":"-"},{"Metabolite":"Aminoadipic acid","HY19_CNT_1":"682960","HY19_CNT_2":"584821","HY19_CNT_3":"298790","HY19_DON_1":"236509","HY19_DON_2":"316976","HY19_DON_3":"339762","HY19_DRP104_1":"218790","HY19_DRP104_2":"305292","HY19_DRP104_3":"186762"},{"Metabolite":"Argininosuccinic acid","HY19_CNT_1":"15469","HY19_CNT_2":"-","HY19_CNT_3":"-","HY19_DON_1":"-","HY19_DON_2":"-","HY19_DON_3":"-","HY19_DRP104_1":"-","HY19_DRP104_2":"-","HY19_DRP104_3":"-"},{"Metabolite":"Beta-Alanine","HY19_CNT_1":"-","HY19_CNT_2":"-","HY19_CNT_3":"-","HY19_DON_1":"-","HY19_DON_2":"-","HY19_DON_3":"-","HY19_DRP104_1":"-","HY19_DRP104_2":"-","HY19_DRP104_3":"-"},{"Metabolite":"Betaine","HY19_CNT_1":"1692776","HY19_CNT_2":"1317169","HY19_CNT_3":"2682132","HY19_DON_1":"1557858","HY19_DON_2":"3690359","HY19_DON_3":"1927674","HY19_DRP104_1":"2463429","HY19_DRP104_2":"2706155","HY19_DRP104_3":"2711772"},{"Metabolite":"Butyrylcarnitine","HY19_CNT_1":"85599","HY19_CNT_2":"76419","HY19_CNT_3":"101867","HY19_DON_1":"141893","HY19_DON_2":"238428","HY19_DON_3":"151479","HY19_DRP104_1":"226972","HY19_DRP104_2":"242034","HY19_DRP104_3":"210855"},{"Metabolite":"Carbamoyl phosphate","HY19_CNT_1":"-","HY19_CNT_2":"-","HY19_CNT_3":"-","HY19_DON_1":"-","HY19_DON_2":"-","HY19_DON_3":"-","HY19_DRP104_1":"-","HY19_DRP104_2":"-","HY19_DRP104_3":"-"},{"Metabolite":"Carnosine","HY19_CNT_1":"55103","HY19_CNT_2":"35007","HY19_CNT_3":"-","HY19_DON_1":"47184","HY19_DON_2":"48642","HY19_DON_3":"39491","HY19_DRP104_1":"-","HY19_DRP104_2":"-","HY19_DRP104_3":"-"},{"Metabolite":"CDP","HY19_CNT_1":"62630","HY19_CNT_2":"85753","HY19_CNT_3":"51419","HY19_DON_1":"-","HY19_DON_2":"23309","HY19_DON_3":"-","HY19_DRP104_1":"-","HY19_DRP104_2":"-","HY19_DRP104_3":"-"},{"Metabolite":"Choline","HY19_CNT_1":"158553632","HY19_CNT_2":"199977424","HY19_CNT_3":"138504064","HY19_DON_1":"160427952","HY19_DON_2":"156923616","HY19_DON_3":"145028944","HY19_DRP104_1":"95457608","HY19_DRP104_2":"141994912","HY19_DRP104_3":"146104784"},{"Metabolite":"cis-Aconitic acid","HY19_CNT_1":"1335604","HY19_CNT_2":"1274576","HY19_CNT_3":"1183327","HY19_DON_1":"741033","HY19_DON_2":"988826","HY19_DON_3":"777735","HY19_DRP104_1":"633783","HY19_DRP104_2":"854536","HY19_DRP104_3":"467337"},{"Metabolite":"Citric 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}

}