{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002892","ANALYSIS_ID":"AN004751","VERSION":"1","CREATED_ON":"October 2, 2023, 8:26 am"},

"PROJECT":{"PROJECT_TITLE":"Folate depletion induces erythroid differentiation through perturbation of de novo purine synthesis","PROJECT_SUMMARY":"Folate, an essential vitamin, is a one-carbon acceptor and donor in key metabolic reactions. Erythroid cells harbor a unique sensitivity to folate deprivation, as revealed by the primary pathological manifestation of nutritional folate deprivation: megaloblastic anemia. To study this metabolic sensitivity, we applied mild folate depletion to human and mouse erythroid cell lines, and primary murine erythroid progenitors. We show that folate depletion induces early blockade of purine synthesis and accumulation of the purine synthesis intermediate and signaling molecule, AICAR, followed by enhanced heme metabolism, hemoglobin synthesis, and erythroid differentiation. This is phenocopied by inhibition of folate metabolism using the SHMT1/2 inhibitor - SHIN1, and by AICAR supplementation. Mechanistically, the metabolically-driven differentiation is independent of nucleotide sensing through mTORC1 and AMPK, and is instead mediated by protein kinase C (PKC). Our findings suggest that folate deprivation-induced premature differentiation of erythroid progenitor cells is a molecular etiology to folate-deficiency induced anemia.","INSTITUTE":"Boston Children''s Hospital, Harvard Medical School","DEPARTMENT":"pathology","LABORATORY":"Kanarek Lab","LAST_NAME":"Kanarek","FIRST_NAME":"Naama","ADDRESS":"Enders 1116.2, 300 Longwood Ave, Boston, MA 02115","EMAIL":"naama.kanarek@childrens.harvard.edu","PHONE":"(617) 355-7433"},

"STUDY":{"STUDY_TITLE":"Folate levels in K562 cells following folate depletion","STUDY_SUMMARY":"Culture of K562 cells in RPMI media containing 100 nM folic acid for up to 8 days followed my metabolomics targeting folate metabolites. All samples were harvested at the same time and the study was set up as a reverse time-course. Day 0 samples were cultured in 2,000 nM folate for the full 8 days.","INSTITUTE":"Boston Children''s Hospital, Harvard Medical School","DEPARTMENT":"pathology","LABORATORY":"Kanarek Lab","LAST_NAME":"Kanarek","FIRST_NAME":"Naama","ADDRESS":"Enders 1116.2, 300 Longwood Ave, Boston, MA 02115","EMAIL":"naama.kanarek@childrens.harvard.edu","PHONE":"6173557433","NUM_GROUPS":"6"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","CELL_STRAIN_DETAILS":"K-562"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day0_Folate_1",
"Factors":{"100nM_Folic_Acid_Growth":"Day0"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM591.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day0_Folate_2",
"Factors":{"100nM_Folic_Acid_Growth":"Day0"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM592.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day0_Folate_3",
"Factors":{"100nM_Folic_Acid_Growth":"Day0"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM593.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day0_Folate_4",
"Factors":{"100nM_Folic_Acid_Growth":"Day0"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM594.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day1_Folate_1",
"Factors":{"100nM_Folic_Acid_Growth":"Day1"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM595.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day1_Folate_2",
"Factors":{"100nM_Folic_Acid_Growth":"Day1"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM596.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day1_Folate_3",
"Factors":{"100nM_Folic_Acid_Growth":"Day1"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM597.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day1_Folate_4",
"Factors":{"100nM_Folic_Acid_Growth":"Day1"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM598.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day2_Folate_1",
"Factors":{"100nM_Folic_Acid_Growth":"Day2"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM599.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day2_Folate_2",
"Factors":{"100nM_Folic_Acid_Growth":"Day2"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM600.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day2_Folate_3",
"Factors":{"100nM_Folic_Acid_Growth":"Day2"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM601.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day2_Folate_4",
"Factors":{"100nM_Folic_Acid_Growth":"Day2"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM602.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day4_Folate_1",
"Factors":{"100nM_Folic_Acid_Growth":"Day4"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM603.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day4_Folate_2",
"Factors":{"100nM_Folic_Acid_Growth":"Day4"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM604.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day4_Folate_3",
"Factors":{"100nM_Folic_Acid_Growth":"Day4"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM605.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day4_Folate_4",
"Factors":{"100nM_Folic_Acid_Growth":"Day4"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM606.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day6_Folate_1",
"Factors":{"100nM_Folic_Acid_Growth":"Day6"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM607.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day6_Folate_2",
"Factors":{"100nM_Folic_Acid_Growth":"Day6"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM608.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day6_Folate_3",
"Factors":{"100nM_Folic_Acid_Growth":"Day6"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM609.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day6_Folate_4",
"Factors":{"100nM_Folic_Acid_Growth":"Day6"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM610.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day8_Folate_1",
"Factors":{"100nM_Folic_Acid_Growth":"Day8"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM611.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day8_Folate_2",
"Factors":{"100nM_Folic_Acid_Growth":"Day8"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM612.raw"}
},
{
"Subject ID":"K562",
"Sample ID":"K562_100nM_Timecourse_Day8_Folate_3",
"Factors":{"100nM_Folic_Acid_Growth":"Day8"},
"Additional sample data":{"RAW_FILE_NAME":"Copy of 20211130_Adam_K562_TC_Folate_AM613.raw"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"One million cells from culture were collected via centrifugation for 20 seconds at 18,000xG, washed with 0.9% NaCl, and collected via centrifugation for 20 seconds at 18,000xG","SAMPLE_TYPE":"Cultured cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"K562 cells were cultured for up to 8 days in either 2,000 nM folic acid or 100 nM folic acid containing RPMI media. Experiment was a reverse time-course. Samples were cultured for the indicated length of time in 100 nM folic acid and harvested on the same date. Day 0 samples were cultured in 2,000 nM folic acid for the full 8 day experiment"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Pellet was resuspended in extraction buffer (80% Methanol, 25 mM Ammonium Acetate and 2.5 mM Na-Ascorbate prepared in LC-MS water, supplemented with isotopically-labelled amino acid standards [Cambridge Isotope Laboratories, MSK-A2-1.2], aminopterin, and reduced glutathione standard [Cambridge Isotope Laboratories, CNLM-6245-10]). Samples were vortexed for 10 sec, then centrifuged for 10 minutes at 18,000 g to pellet cell debris. The supernatant was divided into two tubes and dried on ice using a liquid nitrogen dryer. One tube of dried sample was used for folate metabolite detection. Folate samples were resuspended in reconstitution buffer (0.5% ascorbic acid, 1% K2HPO4 and 0.5% 2-mercaptoethanol) containing rat serum (Sigma, R9759). The rat serum contains the enzymes required to strip the polyglutamate tail from the measured folate. Rat endogenous folate was removed from the rat serum by activated carbon treatment (Sigma, C9157). Polyglutamate tail removal was carried out for 2 h at 37 °C. After the polyglutamate tail removal, the pH was adjusted to 4 using formic acid and samples were loaded on Bond Elute-pH columns (Agilent, 14102062) for a pH-based clean-up. Columns were eluted with 300 μl elution buffer (50% methanol, 0.25% NH4OAc, 0.5% 2-mercaptoethanol), followed by drying the samples using a liquid nitrogen dryer manifold and resuspension in 25 μl water."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"5 μl was injected onto a 2.6 μm, 150 x 3 mm C18 column (Phenomenex, 00F-4462-Y0) equipped with a 3.0 mm safe-guard column (Phenomenex, AJ0-8775). The column oven and autosampler tray were held at 30 °C and 4 °C, respectively. The following conditions were used to achieve chromatographic separation: buffer A was 0.1% formic acid; buffer B was acetonitrile with 0.1% formic acid. The chromatographic gradient was run at a flow rate of 0.250 ml min−1 as follows: 0–5 min: gradient was held at 5% B; 5–10 min: linear gradient of 5% to 36% B; 10.1–14.0 min: linear gradient from 36–95% B; 14.1–18.0 min: gradient was returned to 5% B.","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Phenomenex Kinetex C18 (150 x 3mm,2.6um)","SOLVENT_A":"100% water; 0.1% formic acid","SOLVENT_B":"100% acetonitrile; 0.1% formic acid","FLOW_GRADIENT":"0.250 ml min−1 as follows: 0–5 min: gradient was held at 5% B; 5–10 min: linear gradient of 5% to 36% B; 10.1–14.0 min: linear gradient from 36–95% B; 14.1–18.0 min: gradient was returned to 5% B.","FLOW_RATE":"250 uL/min","COLUMN_TEMPERATURE":"30"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"The mass spectrometer was operated in full-scan, positive ionization mode using three narrow-range scans: 438–450 m/z; 452–462 m/z; and 470–478 m/z, with the resolution set at 70,000, the AGC target at 10e6, and the maximum injection time of 150 ms. HESI settings were: sheath gas flow rate: 40; Aux gas flow rate: 10; Sweep gas: 0; Spray voltage: 2.8 (neg) 3.5 (pos); Capillary temperature 300; S-lens RF level 50; Aux gas heater temp: 350."},

"MS_METABOLITE_DATA":{
"Units":"Arbitrary Units",

"Data":[{"Metabolite":"5,10-Methylenetetrahydrofolate","K562_100nM_Timecourse_Day0_Folate_1":"90107.64217","K562_100nM_Timecourse_Day0_Folate_2":"82790.79124","K562_100nM_Timecourse_Day0_Folate_3":"110023.1188","K562_100nM_Timecourse_Day0_Folate_4":"188542.8353","K562_100nM_Timecourse_Day1_Folate_1":"86129.17182","K562_100nM_Timecourse_Day1_Folate_2":"205292.1885","K562_100nM_Timecourse_Day1_Folate_3":"47926.19904","K562_100nM_Timecourse_Day1_Folate_4":"28353.69465","K562_100nM_Timecourse_Day2_Folate_1":"27600.48078","K562_100nM_Timecourse_Day2_Folate_2":"34811.56416","K562_100nM_Timecourse_Day2_Folate_3":"31315.71011","K562_100nM_Timecourse_Day2_Folate_4":"68633.86958","K562_100nM_Timecourse_Day4_Folate_1":"24854.02712","K562_100nM_Timecourse_Day4_Folate_2":"36405.83868","K562_100nM_Timecourse_Day4_Folate_3":"173408.149","K562_100nM_Timecourse_Day4_Folate_4":"19941.01383","K562_100nM_Timecourse_Day6_Folate_1":"29255.6949","K562_100nM_Timecourse_Day6_Folate_2":"41601.52081","K562_100nM_Timecourse_Day6_Folate_3":"67376.04889","K562_100nM_Timecourse_Day6_Folate_4":"32147.72662","K562_100nM_Timecourse_Day8_Folate_1":"36169.9172","K562_100nM_Timecourse_Day8_Folate_2":"28536.88366","K562_100nM_Timecourse_Day8_Folate_3":"63411.02661"},{"Metabolite":"Folic Acid","K562_100nM_Timecourse_Day0_Folate_1":"742139.1736","K562_100nM_Timecourse_Day0_Folate_2":"859811.0433","K562_100nM_Timecourse_Day0_Folate_3":"878147.3172","K562_100nM_Timecourse_Day0_Folate_4":"911221.066","K562_100nM_Timecourse_Day1_Folate_1":"108751.9155","K562_100nM_Timecourse_Day1_Folate_2":"255701.5607","K562_100nM_Timecourse_Day1_Folate_3":"96759.78345","K562_100nM_Timecourse_Day1_Folate_4":"75850.19844","K562_100nM_Timecourse_Day2_Folate_1":"109395.983","K562_100nM_Timecourse_Day2_Folate_2":"120204.2362","K562_100nM_Timecourse_Day2_Folate_3":"116794.4772","K562_100nM_Timecourse_Day2_Folate_4":"154412.2259","K562_100nM_Timecourse_Day4_Folate_1":"86061.31882","K562_100nM_Timecourse_Day4_Folate_2":"104839.323","K562_100nM_Timecourse_Day4_Folate_3":"270852.2535","K562_100nM_Timecourse_Day4_Folate_4":"85754.842","K562_100nM_Timecourse_Day6_Folate_1":"118292.1775","K562_100nM_Timecourse_Day6_Folate_2":"124181.8066","K562_100nM_Timecourse_Day6_Folate_3":"126383.6301","K562_100nM_Timecourse_Day6_Folate_4":"134083.622","K562_100nM_Timecourse_Day8_Folate_1":"118814.2147","K562_100nM_Timecourse_Day8_Folate_2":"119922.3329","K562_100nM_Timecourse_Day8_Folate_3":"160343.6091"},{"Metabolite":"5-methyltetrahydrofolate","K562_100nM_Timecourse_Day0_Folate_1":"928100.2985","K562_100nM_Timecourse_Day0_Folate_2":"954688.0815","K562_100nM_Timecourse_Day0_Folate_3":"1226331.188","K562_100nM_Timecourse_Day0_Folate_4":"1404617.031","K562_100nM_Timecourse_Day1_Folate_1":"552192.7623","K562_100nM_Timecourse_Day1_Folate_2":"1408679.155","K562_100nM_Timecourse_Day1_Folate_3":"457393.5517","K562_100nM_Timecourse_Day1_Folate_4":"417083.6811","K562_100nM_Timecourse_Day2_Folate_1":"224884.5465","K562_100nM_Timecourse_Day2_Folate_2":"215422.243","K562_100nM_Timecourse_Day2_Folate_3":"304345.2492","K562_100nM_Timecourse_Day2_Folate_4":"356587.2379","K562_100nM_Timecourse_Day4_Folate_1":"163320.2897","K562_100nM_Timecourse_Day4_Folate_2":"195050.2765","K562_100nM_Timecourse_Day4_Folate_3":"834202.6318","K562_100nM_Timecourse_Day4_Folate_4":"136884.4686","K562_100nM_Timecourse_Day6_Folate_1":"180425.5321","K562_100nM_Timecourse_Day6_Folate_2":"267382.1686","K562_100nM_Timecourse_Day6_Folate_3":"365604.7184","K562_100nM_Timecourse_Day6_Folate_4":"157208.6302","K562_100nM_Timecourse_Day8_Folate_1":"150960.6169","K562_100nM_Timecourse_Day8_Folate_2":"173876.0236","K562_100nM_Timecourse_Day8_Folate_3":"236065.5345"},{"Metabolite":"tetrahydrofolate","K562_100nM_Timecourse_Day0_Folate_1":"41798.64209","K562_100nM_Timecourse_Day0_Folate_2":"46981.61984","K562_100nM_Timecourse_Day0_Folate_3":"60553.55877","K562_100nM_Timecourse_Day0_Folate_4":"106663.4481","K562_100nM_Timecourse_Day1_Folate_1":"36905.45694","K562_100nM_Timecourse_Day1_Folate_2":"127561.4832","K562_100nM_Timecourse_Day1_Folate_3":"37595.54893","K562_100nM_Timecourse_Day1_Folate_4":"22852.70507","K562_100nM_Timecourse_Day2_Folate_1":"18998.9814","K562_100nM_Timecourse_Day2_Folate_2":"19982.6599","K562_100nM_Timecourse_Day2_Folate_3":"30642.99522","K562_100nM_Timecourse_Day2_Folate_4":"34211.63345","K562_100nM_Timecourse_Day4_Folate_1":"17948.52998","K562_100nM_Timecourse_Day4_Folate_2":"25662.69992","K562_100nM_Timecourse_Day4_Folate_3":"107023.4577","K562_100nM_Timecourse_Day4_Folate_4":"9004.44102","K562_100nM_Timecourse_Day6_Folate_1":"20488.99466","K562_100nM_Timecourse_Day6_Folate_2":"24995.09831","K562_100nM_Timecourse_Day6_Folate_3":"49578.48247","K562_100nM_Timecourse_Day6_Folate_4":"19434.39951","K562_100nM_Timecourse_Day8_Folate_1":"19612.31829","K562_100nM_Timecourse_Day8_Folate_2":"17787.00148","K562_100nM_Timecourse_Day8_Folate_3":"25401.60128"},{"Metabolite":"5-formyltetrahydrofolate","K562_100nM_Timecourse_Day0_Folate_1":"91975.97051","K562_100nM_Timecourse_Day0_Folate_2":"127792.5144","K562_100nM_Timecourse_Day0_Folate_3":"180650.4822","K562_100nM_Timecourse_Day0_Folate_4":"421804.2675","K562_100nM_Timecourse_Day1_Folate_1":"229964.56","K562_100nM_Timecourse_Day1_Folate_2":"1274044.488","K562_100nM_Timecourse_Day1_Folate_3":"150210.6502","K562_100nM_Timecourse_Day1_Folate_4":"34628.86819","K562_100nM_Timecourse_Day2_Folate_1":"71103.83023","K562_100nM_Timecourse_Day2_Folate_2":"64186.1269","K562_100nM_Timecourse_Day2_Folate_3":"146412.9491","K562_100nM_Timecourse_Day2_Folate_4":"170629.8228","K562_100nM_Timecourse_Day4_Folate_1":"116614.542","K562_100nM_Timecourse_Day4_Folate_2":"156736.7884","K562_100nM_Timecourse_Day4_Folate_3":"865435.393","K562_100nM_Timecourse_Day4_Folate_4":"72121.53158","K562_100nM_Timecourse_Day6_Folate_1":"162106.6758","K562_100nM_Timecourse_Day6_Folate_2":"223898.0367","K562_100nM_Timecourse_Day6_Folate_3":"402593.751","K562_100nM_Timecourse_Day6_Folate_4":"107110.7224","K562_100nM_Timecourse_Day8_Folate_1":"105824.0089","K562_100nM_Timecourse_Day8_Folate_2":"124704.1297","K562_100nM_Timecourse_Day8_Folate_3":"195119.8488"},{"Metabolite":"10-formyltetrahydrofolate","K562_100nM_Timecourse_Day0_Folate_1":"14818.17363","K562_100nM_Timecourse_Day0_Folate_2":"17374.38713","K562_100nM_Timecourse_Day0_Folate_3":"16710.40786","K562_100nM_Timecourse_Day0_Folate_4":"20134.34932","K562_100nM_Timecourse_Day1_Folate_1":"15003.28747","K562_100nM_Timecourse_Day1_Folate_2":"31504.19885","K562_100nM_Timecourse_Day1_Folate_3":"9286.931226","K562_100nM_Timecourse_Day1_Folate_4":"9036.376819","K562_100nM_Timecourse_Day2_Folate_1":"8812.651898","K562_100nM_Timecourse_Day2_Folate_2":"7899.263499","K562_100nM_Timecourse_Day2_Folate_3":"11877.10229","K562_100nM_Timecourse_Day2_Folate_4":"12136.42615","K562_100nM_Timecourse_Day4_Folate_1":"9309.455325","K562_100nM_Timecourse_Day4_Folate_2":"5877.715547","K562_100nM_Timecourse_Day4_Folate_3":"19455.87405","K562_100nM_Timecourse_Day4_Folate_4":"7403.363518","K562_100nM_Timecourse_Day6_Folate_1":"7814.018367","K562_100nM_Timecourse_Day6_Folate_2":"6562.24426","K562_100nM_Timecourse_Day6_Folate_3":"10994.06809","K562_100nM_Timecourse_Day6_Folate_4":"4123.690263","K562_100nM_Timecourse_Day8_Folate_1":"4774.447611","K562_100nM_Timecourse_Day8_Folate_2":"5805.944038","K562_100nM_Timecourse_Day8_Folate_3":"7267.169383"}],

"Metabolites":[{"Metabolite":"5,10-Methylenetetrahydrofolate","Standardized name":"-","Formula":"-","Exact mass":"-","Sub class":"-"},{"Metabolite":"Folic Acid","Standardized name":"Folic acid","Formula":"C19H19N7O6","Exact mass":"441.1397","Sub class":"Folic acids"},{"Metabolite":"5-methyltetrahydrofolate","Standardized name":"5-Methyltetrahydrofolic acid","Formula":"C20H25N7O6","Exact mass":"459.1866","Sub class":"Pterins"},{"Metabolite":"tetrahydrofolate","Standardized name":"6R-THF","Formula":"C19H23N7O6","Exact mass":"445.1710","Sub class":"Tetrahydrofolic acids"},{"Metabolite":"5-formyltetrahydrofolate","Standardized name":"-","Formula":"-","Exact mass":"-","Sub class":"-"},{"Metabolite":"10-formyltetrahydrofolate","Standardized name":"N10-Formyl-THF","Formula":"C20H23N7O7","Exact mass":"473.1659","Sub class":"Tetrahydrofolic acids"}]
}

}