{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002961","ANALYSIS_ID":"AN004862","VERSION":"1","CREATED_ON":"November 3, 2023, 6:06 am"},

"PROJECT":{"PROJECT_TITLE":"Mid-Old Cells are Potential Target for Anti-aging Interventions in the Elderly","PROJECT_SUMMARY":"The biological process of aging is thought to result in part from accumulation of senescent cells in organs. However, the present study identified a subset of fibroblasts and smooth muscle cells which are the major constituents of organ stroma neither proliferative nor senescent in tissues of the elderly, which we termed “mid-old status” cells. Upregulation of pro-inflammatory genes (IL1B and SAA1) and downregulation of anti-inflammatory genes (SLIT2 and CXCL12) were detected in mid-old cells. In the stroma, SAA1 promotes development of the inflammatory microenvironment via upregulation of MMP9, which decreases the stability of epithelial cells present on the basement membrane, decreasing epithelial cell function. Remarkably, the microenvironmental change and the functional decline of mid-old cells could be reversed by a young cell-originated protein, SLIT2. Our data identify functional reversion of mid-old cells as a potential method to prevent or ameliorate aspects of aging-related tissue dysfunction.","INSTITUTE":"Ajou University Medical Center","LAST_NAME":"Kim","FIRST_NAME":"Young Hwa","ADDRESS":"206, World cup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea","EMAIL":"skyblue32@nate.com","PHONE":"+82-10-5153-3636"},

"STUDY":{"STUDY_TITLE":"Analysis of Metabolites Secreted from Fibroblast Young Cells","STUDY_SUMMARY":"In this experimental study, we aimed to uncover the factors in young cell secretions that trigger the reverse aging of mid-old cells, co-culturing them and observing a striking transformation, although we could not identify the specific factors responsible for this rejuvenation","INSTITUTE":"Ajou University Medical Center","LAST_NAME":"Kim","FIRST_NAME":"Young Hwa","ADDRESS":"206, World cup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea","EMAIL":"skyblue32@nate.com","PHONE":"+82-10-5153-3636"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"20210624_AJU_Y1",
"Factors":{"*Factor":"Test sample"},
"Additional sample data":{"RAW_FILE_NAME":"20210624_AJU_Y1.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"20210624_AJU_Y2",
"Factors":{"*Factor":"Test sample"},
"Additional sample data":{"RAW_FILE_NAME":"20210624_AJU_Y2.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"20210624_AJU_Y3",
"Factors":{"*Factor":"Test sample"},
"Additional sample data":{"RAW_FILE_NAME":"20210624_AJU_Y3.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"20210624_AJU_M1",
"Factors":{"*Factor":"Control sample"},
"Additional sample data":{"RAW_FILE_NAME":"20210624_AJU_M1.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"20210624_AJU_M2",
"Factors":{"*Factor":"Control sample"},
"Additional sample data":{"RAW_FILE_NAME":"20210624_AJU_M2.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"20210624_AJU_M3",
"Factors":{"*Factor":"Control sample"},
"Additional sample data":{"RAW_FILE_NAME":"20210624_AJU_M3.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"blank_20210624",
"Factors":{"*Factor":"Non"},
"Additional sample data":{"RAW_FILE_NAME":"blank_20210624.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"The experiment was initiated by seeding the cells in a 100mm cell culture dish, followed by a 2-day incubation period, during which the culture medium was carefully extracted from the cell culture dish. Use a sterile technique to minimize contamination. Transfer the harvested culture medium to a suitable container.","SAMPLE_TYPE":"Cultured fibroblasts"},

"TREATMENT":{"TREATMENT_SUMMARY":"no treatment"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Metabolites were extracted using 80% methanol. In brief, the samples were added with 80% methanol. After vortexing for 1 min and centrifugation at 2000×g for 10 min, supernatant was transferred to a new 1.5 mL tube and completely dried using a HyperVAC-MAX VC2200 centrifugal vacuum concentrator (Hanil Scientific Inc., Korea). Dried metabolite contents were reconstituted in 100 µL of 0.1% formic acid in water and then subjected to LC-MS/MS analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Q Exactive™ Hybrid Quadrupole-Orbitrap MS coupled with a 1290 Infinity UHPLC","COLUMN_NAME":"ZORBAX Eclipse Plus C18 Rapid Resolution High Definition (RRHD) column","SOLVENT_A":"0.1% formic acid in water","SOLVENT_B":"0.1% formic acid in 80% acetonitrile","FLOW_GRADIENT":"2.5% solvent B in 5 min, 2.5–12.5% solvent B in 29 min, 12.5–25% solvent B in 11 min, 25–37.5% solvent B in 11 min, 37.5-80% solvent B in 0.1 min, holding at 80% of solvent B in 13.9 min, 80–2.5% solvent B in 0.1 min, 2.5% solvent B for 19.9 min","FLOW_RATE":"0.2 mL/min","COLUMN_TEMPERATURE":"320℃"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Q Exactive™ Hybrid Quadrupole-Orbitrap MS coupled with a 1290 Infinity UHPLC","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Obtained UHPLC-Orbitrap-MS/MS RAW files were processed using Compound Discoverer 3.1.1.12TM (Thermo Fisher Scientific, Waltham, MA, USA). Untargeted metabolomics workflow was used to perform retention time alignment and compound identification. Identification of compounds using mzCloud and ChemSpider","MS_RESULTS_FILE":"ST002961_AN004862_Results.txt UNITS:Peak area Has m/z:Yes Has RT:Yes RT units:Minutes"}

}