#METABOLOMICS WORKBENCH Daikin_20231210_171337 DATATRACK_ID:4497 STUDY_ID:ST003001 ANALYSIS_ID:AN004929
VERSION                          	1
CREATED_ON                       	12-12-2023
#PROJECT
PR:PROJECT_TITLE                 	The Track Dairy Cattle (trackDC) study
PR:PROJECT_SUMMARY               	The Track Dairy Cattle (trackDC) study is a longitudinal study in northern China
PR:PROJECT_SUMMARY               	that aims to track newborn calves to assess the development of gut microbiota
PR:PROJECT_SUMMARY               	during early life that contributes to cattle health and production. In this
PR:PROJECT_SUMMARY               	study, 36 newborn calves were randomly assigned to three groups and followed for
PR:PROJECT_SUMMARY               	two months after birth. The groups included a control group (CON), a rumen
PR:PROJECT_SUMMARY               	microbiota transplantation group (RMT), and a rumen fluid transplantation group
PR:PROJECT_SUMMARY               	(RFT) and intensive data has been collected. Blood samples were collected at 15,
PR:PROJECT_SUMMARY               	35, and 56 days after birth.
PR:INSTITUTE                     	Nanjing Medical University
PR:LAST_NAME                     	Dong
PR:FIRST_NAME                    	Quanbin
PR:ADDRESS                       	No. 215 Guangzhou road
PR:EMAIL                         	qbdong@stu.njmu.edu.cn
PR:PHONE                         	15311095435
PR:DOI                           	http://dx.doi.org/10.21228/M8VX4G
#STUDY
ST:STUDY_TITLE                   	The Track Dairy Cattle (trackDC) study
ST:STUDY_SUMMARY                 	The Track Dairy Cattle (trackDC) study is a longitudinal study in northern China
ST:STUDY_SUMMARY                 	that aims to track newborn calves to assess the development of gut microbiota
ST:STUDY_SUMMARY                 	during early life that contributes to cattle health and production. In this
ST:STUDY_SUMMARY                 	study, 36 newborn calves were randomly assigned to three groups and followed for
ST:STUDY_SUMMARY                 	two months after birth. The groups included a control group (CON), a rumen
ST:STUDY_SUMMARY                 	microbiota transplantation group (RMT), and a rumen fluid transplantation group
ST:STUDY_SUMMARY                 	(RFT) and intensive data has been collected. Blood samples were collected at 15,
ST:STUDY_SUMMARY                 	35, and 56 days after birth.
ST:INSTITUTE                     	Nanjing Medical University
ST:LAST_NAME                     	Dong
ST:FIRST_NAME                    	Quanbin
ST:ADDRESS                       	No. 215 Guangzhou road
ST:EMAIL                         	qbdong@stu.njmu.edu.cn
ST:PHONE                         	15311095435
ST:SUBMIT_DATE                   	2023-12-10
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Bos taurus
SU:TAXONOMY_ID                   	9913
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	F62_0	Transplantation group:CON | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F62_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F63_0	Transplantation group:CON | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F63_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F64_0	Transplantation group:CON | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F64_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F65_0	Transplantation group:CON | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F65_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F84_0	Transplantation group:CON | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F84_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F86_0	Transplantation group:CON | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F86_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F87_0	Transplantation group:CON | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F87_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F88_0	Transplantation group:CON | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F88_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F89_0	Transplantation group:CON | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F89_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F90_0	Transplantation group:CON | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F90_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F91_0	Transplantation group:CON | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F91_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F92_0	Transplantation group:CON | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F92_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F62_15	Transplantation group:CON | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F62_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F63_15	Transplantation group:CON | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F63_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F64_15	Transplantation group:CON | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F64_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F65_15	Transplantation group:CON | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F65_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F84_15	Transplantation group:CON | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F84_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F86_15	Transplantation group:CON | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F86_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F87_15	Transplantation group:CON | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F87_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F88_15	Transplantation group:CON | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F88_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F89_15	Transplantation group:CON | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F89_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F90_15	Transplantation group:CON | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F90_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F91_15	Transplantation group:CON | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F91_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F92_15	Transplantation group:CON | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F92_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F62_35	Transplantation group:CON | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F62_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F63_35	Transplantation group:CON | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F63_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F64_35	Transplantation group:CON | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F64_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F65_35	Transplantation group:CON | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F65_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F84_35	Transplantation group:CON | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F84_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F86_35	Transplantation group:CON | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F86_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F87_35	Transplantation group:CON | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F87_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F88_35	Transplantation group:CON | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F88_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F89_35	Transplantation group:CON | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F89_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F90_35	Transplantation group:CON | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F90_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F91_35	Transplantation group:CON | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F91_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F92_35	Transplantation group:CON | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F92_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F62_56	Transplantation group:CON | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F62_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F63_56	Transplantation group:CON | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F63_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F64_56	Transplantation group:CON | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F64_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F65_56	Transplantation group:CON | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F65_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F84_56	Transplantation group:CON | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F84_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F86_56	Transplantation group:CON | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F86_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F87_56	Transplantation group:CON | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F87_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F88_56	Transplantation group:CON | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F88_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F89_56	Transplantation group:CON | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F89_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F90_56	Transplantation group:CON | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F90_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F91_56	Transplantation group:CON | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F91_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F92_56	Transplantation group:CON | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F92_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F66_0	Transplantation group:RFT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F66_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F67_0	Transplantation group:RFT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F67_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F68_0	Transplantation group:RFT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F68_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F69_0	Transplantation group:RFT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F69_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F70_0	Transplantation group:RFT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F70_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F73_0	Transplantation group:RFT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F73_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F75_0	Transplantation group:RFT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F75_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F76_0	Transplantation group:RFT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F76_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F77_0	Transplantation group:RFT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F77_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F79_0	Transplantation group:RFT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F79_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F80_0	Transplantation group:RFT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F80_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F83_0	Transplantation group:RFT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F83_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F66_15	Transplantation group:RFT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F66_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F67_15	Transplantation group:RFT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F67_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F68_15	Transplantation group:RFT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F68_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F69_15	Transplantation group:RFT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F69_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F70_15	Transplantation group:RFT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F70_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F73_15	Transplantation group:RFT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F73_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F75_15	Transplantation group:RFT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F75_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F76_15	Transplantation group:RFT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F76_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F77_15	Transplantation group:RFT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F77_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F79_15	Transplantation group:RFT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F79_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F80_15	Transplantation group:RFT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F80_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F83_15	Transplantation group:RFT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F83_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F66_35	Transplantation group:RFT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F66_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F67_35	Transplantation group:RFT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F67_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F68_35	Transplantation group:RFT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F68_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F69_35	Transplantation group:RFT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F69_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F70_35	Transplantation group:RFT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F70_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F73_35	Transplantation group:RFT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F73_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F75_35	Transplantation group:RFT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F75_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F76_35	Transplantation group:RFT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F76_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F77_35	Transplantation group:RFT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F77_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F79_35	Transplantation group:RFT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F79_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F80_35	Transplantation group:RFT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F80_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F83_35	Transplantation group:RFT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F83_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F66_56	Transplantation group:RFT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F66_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F67_56	Transplantation group:RFT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F67_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F68_56	Transplantation group:RFT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F68_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F69_56	Transplantation group:RFT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F69_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F70_56	Transplantation group:RFT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F70_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F73_56	Transplantation group:RFT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F73_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F75_56	Transplantation group:RFT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F75_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F76_56	Transplantation group:RFT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F76_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F77_56	Transplantation group:RFT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F77_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F79_56	Transplantation group:RFT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F79_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F80_56	Transplantation group:RFT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F80_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F83_56	Transplantation group:RFT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F83_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F39_0	Transplantation group:RMT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F39_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F41_0	Transplantation group:RMT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F41_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F47_0	Transplantation group:RMT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F47_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F48_0	Transplantation group:RMT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F48_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F49_0	Transplantation group:RMT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F49_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F52_0	Transplantation group:RMT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F52_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F53_0	Transplantation group:RMT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F53_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F55_0	Transplantation group:RMT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F55_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F56_0	Transplantation group:RMT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F56_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F58_0	Transplantation group:RMT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F58_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F61_0	Transplantation group:RMT | collection_timepoint:0	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F61_0.raw
SUBJECT_SAMPLE_FACTORS           	-	F39_15	Transplantation group:RMT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F39_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F41_15	Transplantation group:RMT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F41_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F47_15	Transplantation group:RMT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F47_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F48_15	Transplantation group:RMT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F48_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F49_15	Transplantation group:RMT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F49_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F52_15	Transplantation group:RMT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F52_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F53_15	Transplantation group:RMT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F53_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F55_15	Transplantation group:RMT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F55_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F56_15	Transplantation group:RMT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F56_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F58_15	Transplantation group:RMT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F58_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F61_15	Transplantation group:RMT | collection_timepoint:15	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F61_15.raw
SUBJECT_SAMPLE_FACTORS           	-	F39_35	Transplantation group:RMT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F39_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F41_35	Transplantation group:RMT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F41_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F47_35	Transplantation group:RMT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F47_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F48_35	Transplantation group:RMT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F48_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F49_35	Transplantation group:RMT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F49_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F52_35	Transplantation group:RMT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F52_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F53_35	Transplantation group:RMT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F53_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F55_35	Transplantation group:RMT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F55_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F56_35	Transplantation group:RMT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F56_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F58_35	Transplantation group:RMT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F58_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F61_35	Transplantation group:RMT | collection_timepoint:35	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F61_35.raw
SUBJECT_SAMPLE_FACTORS           	-	F39_56	Transplantation group:RMT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F39_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F41_56	Transplantation group:RMT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F41_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F47_56	Transplantation group:RMT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F47_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F48_56	Transplantation group:RMT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F48_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F49_56	Transplantation group:RMT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F49_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F52_56	Transplantation group:RMT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F52_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F53_56	Transplantation group:RMT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F53_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F55_56	Transplantation group:RMT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F55_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F56_56	Transplantation group:RMT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F56_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F58_56	Transplantation group:RMT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F58_56.raw
SUBJECT_SAMPLE_FACTORS           	-	F61_56	Transplantation group:RMT | collection_timepoint:56	organism_part=blood plasma; Unit=day; RAW_FILE_NAME=F61_56.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	In this study, 36 newborn calves were randomly assigned to three groups and
CO:COLLECTION_SUMMARY            	followed for two months after birth. The groups included a control group (CON),
CO:COLLECTION_SUMMARY            	a rumen microbiota transplantation group (RMT), and a rumen fluid
CO:COLLECTION_SUMMARY            	transplantation group (RFT) and intensive data has been collected. The newborn
CO:COLLECTION_SUMMARY            	calves were trained to feed milk using a bucket and then transferred to
CO:COLLECTION_SUMMARY            	individual calf hutches. Starter was provided ad libitum three days after birth
CO:COLLECTION_SUMMARY            	and once daily in the morning thereafter. Pasteurized whole milk was fed twice
CO:COLLECTION_SUMMARY            	daily at 0800 and 1800 h using a bucket, and the calves were weaned 56 days
CO:COLLECTION_SUMMARY            	after birth. RMT and RFT were performed by veterinarians, where the ruminal
CO:COLLECTION_SUMMARY            	fluid used in RMT and RFT was collected from a healthy cattle (4-year-old,
CO:COLLECTION_SUMMARY            	600kg, in the dry period,) with a permanent rumen cannula 2 hours after the
CO:COLLECTION_SUMMARY            	morning feed. Fresh ruminal fluid was mixed with raw milk and fed to the calves
CO:COLLECTION_SUMMARY            	in the RMT group immediately after collection. For the RFT group, the ruminal
CO:COLLECTION_SUMMARY            	fluid was autoclaved before feeding. A volume of 50 mL, 80 mL, and 110 mL of
CO:COLLECTION_SUMMARY            	ruminal fluid was fed from day 7 to day 11, day 21 to day 25, and day 42 to day
CO:COLLECTION_SUMMARY            	46, respectively. Fecal and blood samples were collected at 15, 35, and 56 days
CO:COLLECTION_SUMMARY            	after birth.
CO:COLLECTION_PROTOCOL_FILENAME  	collection_protocol.docx
CO:SAMPLE_TYPE                   	Blood (plasma)
#TREATMENT
TR:TREATMENT_SUMMARY             	The newborn calves were trained to feed milk using a bucket and then transferred
TR:TREATMENT_SUMMARY             	to individual calf hutches. Starter was provided ad libitum three days after
TR:TREATMENT_SUMMARY             	birth and once daily in the morning thereafter. Pasteurized whole milk was fed
TR:TREATMENT_SUMMARY             	twice daily at 0800 and 1800 h using a bucket, and the calves were weaned 56
TR:TREATMENT_SUMMARY             	days after birth. RMT and RFT were performed by veterinarians, where the ruminal
TR:TREATMENT_SUMMARY             	fluid used in RMT and RFT was collected from a healthy cattle (4-year-old,
TR:TREATMENT_SUMMARY             	600kg, in the dry period,) with a permanent rumen cannula 2 hours after the
TR:TREATMENT_SUMMARY             	morning feed. Fresh ruminal fluid was mixed with raw milk and fed to the calves
TR:TREATMENT_SUMMARY             	in the RMT group immediately after collection. For the RFT group, the ruminal
TR:TREATMENT_SUMMARY             	fluid was autoclaved before feeding. A volume of 50 mL, 80 mL, and 110 mL of
TR:TREATMENT_SUMMARY             	ruminal fluid was fed from day 7 to day 11, day 21 to day 25, and day 42 to day
TR:TREATMENT_SUMMARY             	46, respectively. Fecal and blood samples were collected at 15, 35, and 56 days
TR:TREATMENT_SUMMARY             	after birth.
TR:TREATMENT_PROTOCOL_FILENAME   	treatment_protocol.docx
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	-
SP:SAMPLEPREP_PROTOCOL_FILENAME  	sampleprep_protocol.docx
#CHROMATOGRAPHY
CH:METHODS_FILENAME              	UHPLC-MSMS_Analysis.docx
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Thermo Hypersil GOLD aQ (100 x 2.1mm,1.9um)
CH:COLUMN_TEMPERATURE            	40℃
CH:FLOW_GRADIENT                 	2% B, 1.5 min; 2-85% B, 3 min; 100% B, 10 min;100-2% B, 10.1 min;2% B, 12
CH:FLOW_GRADIENT                 	min
CH:FLOW_RATE                     	0.2 mL/min
CH:SOLVENT_A                     	100% water; 0.1% formic acid
CH:SOLVENT_B                     	100% methanol
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive HF hybrid Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:MS_COMMENTS                   	Q ExactiveTM HF mass spectrometer was operated in positive/negative polarity
MS:MS_COMMENTS                   	mode with spray voltage of 3.2 kV, capillary temperature of 320°C, sheath gas
MS:MS_COMMENTS                   	flow rate of 40 arb and aux gas flow rate of 10 arb, Funnel RF level of 40, Aux
MS:MS_COMMENTS                   	gas heater temperature of 350°C.
MS:ION_MODE                      	UNSPECIFIED
MS:MS_RESULTS_FILE               	ST003001_AN004929_Results.txt	UNITS:s	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END