{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001936","ANALYSIS_ID":"AN004932","VERSION":"1","CREATED_ON":"December 12, 2023, 3:11 pm"}, "PROJECT":{"PROJECT_TITLE":"Pseudoexfoliation aqueous humor lipidome suggests enrichment of specific pathways","PROJECT_SUMMARY":"Lipids were identified and quantified with LipidSearch 4.2.21. Statistical analysis was conducted through MetaboAnalyst 5.0.","INSTITUTE":"University of Miami","DEPARTMENT":"Ophthalmology","LAST_NAME":"Bhattacharya","FIRST_NAME":"Sanjoy K.","ADDRESS":"1638 NW 10th Avenue, Room 706-A, Miami, FL 33136","EMAIL":"sbhattacharya@med.miami.edu","PHONE":"3054824103"}, "STUDY":{"STUDY_TITLE":"Pseudoexfoliation aqueous humor lipidome suggests enrichment of specific pathways","STUDY_SUMMARY":"Pseudoexfoliation syndrome (PEX) is systemic disorder that manifests as white, fluffy, proteinaceous fibrillar material throughout the body. In the eye such deposits result in Pseudoexfoliation glaucoma (PEXG), due to impeding aqueous humor outflow. Serum lipid alterations and increased lipid peroxidation have been reported in PEX. We report first ever comprehensive lipid profiling of the aqueous humor (AH) of PEXG. Our untargeted lipidomic analysis of 23 non-glaucomatous control, 19 primary open angle glaucoma, 9 PEX, and 14 PEXG AH with 13 deuterated lipid internal standards for normalization among the lipid classes resulted in the combined identification of 489 lipid species within 26 lipid classes across PEX, PEXG, POAG, and control AH. The mean total lipid content in the AH across samples showed that control AH (mean peak area 13.54 ± 56.1) had, on average, greater total lipid content than PEX (4.21 ± 10.90), PEXG (9.08 ± 25.97), and POAG (5.66 ± 15.75) samples. Multiple cholesterol esters (ChE), phosphatidylcholines (PC), triglycerides (TG), and ceramides (Cer) were present in higher concentrations for the PEXG AH samples. Some of the lipids found in high concentrations in the PEXG samples are ChE(16:0), ChE(20:3), ChE(18:1), ChE(18:3), ChE(22:6), ChE(18:2), ChE(20:4), PC(16:0/16:0), PC(16:0/18:2), TG(18:1/18:1/20:4), and Cer(t18:0/24:0). The CerG2GNAc1(d34:1) was enriched in control samples and depleted both in PEX and PEXG samples. The PC (18:0/18:2), PC (36:2), and PC (34:1e) are in low concentrations for PEX but highly concentrated in PEXG, despite both having similar material deposits, suggesting they are fundamentally different in composition. Elevations in Apolipoprotein A-I (APOA1) correlated to increase abundance of PC lipid species in the AH of patients with PEXG. Machine learning prediction with three supervised logistic regression binary classification tasks showed 1) POAG vs control, with 86% accuracy 2) PEXG vs control, with 71% accuracy and 3) PEX vs control, with 86% accuracy, respectively.","INSTITUTE":"University of Miami","DEPARTMENT":"Ophthalmology","LAST_NAME":"Bhattacharya","FIRST_NAME":"Sanjoy K.","ADDRESS":"1638 NW 10th Avenue, Room 706-A, Miami, FL 33136","EMAIL":"sbhattacharya@med.miami.edu","PHONE":"3054824103"}, "SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","GENDER":"Male and female"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"BB03", "Factors":{"Factor":"PEX"}, "Additional sample data":{"RAW_FILE_NAME":"3_POS.raw","Other":"3_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"BB04", "Factors":{"Factor":"PEX"}, "Additional sample data":{"RAW_FILE_NAME":"4_POS.raw","Other":"4_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX1", "Factors":{"Factor":"PEX"}, "Additional sample data":{"RAW_FILE_NAME":"5_POS.raw","Other":"5_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX3", "Factors":{"Factor":"PEX"}, "Additional sample data":{"RAW_FILE_NAME":"7_POS.raw","Other":"7_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX5", "Factors":{"Factor":"PEX"}, "Additional sample data":{"RAW_FILE_NAME":"9_POS.raw","Other":"9_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX10", "Factors":{"Factor":"PEX"}, "Additional sample data":{"RAW_FILE_NAME":"14_POS.raw","Other":"14_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX12", "Factors":{"Factor":"PEX"}, "Additional sample data":{"RAW_FILE_NAME":"16_POS.raw","Other":"16_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX13", "Factors":{"Factor":"PEX"}, "Additional sample data":{"RAW_FILE_NAME":"17_POS.raw","Other":"17_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX18", "Factors":{"Factor":"PEX"}, "Additional sample data":{"RAW_FILE_NAME":"22_POS.raw","Other":"22_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"BB02", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"2_POS.raw","Other":"2_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX2", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"6_POS.raw","Other":"6_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX4", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"8_POS.raw","Other":"8_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX6", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"10_POS.raw","Other":"10_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX7", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"11_POS.raw","Other":"11_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX8", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"12_POS.raw","Other":"12_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX9", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"13_POS.raw","Other":"13_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX11", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"15_POS.raw","Other":"15_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX14", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"18_POS.raw","Other":"18_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX15", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"19_POS.raw","Other":"19_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX16", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"20_POS.raw","Other":"20_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX17", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"21_POS.raw","Other":"21_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX19", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"23_POS.raw","Other":"23_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"PX20", "Factors":{"Factor":"PEXG"}, "Additional sample data":{"RAW_FILE_NAME":"24_POS.raw","Other":"24_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G1", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"25_POS.raw","Other":"25_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G2", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"26_POS.raw","Other":"26_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G3", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"27_POS.raw","Other":"27_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G4", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"28_POS.raw","Other":"28_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G5", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"29_POS.raw","Other":"29_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G6", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"30_POS.raw","Other":"30_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G7", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"31_POS.raw","Other":"31_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G8", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"32_POS.raw","Other":"32_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G9", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"33_POS.raw","Other":"33_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G10", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"34_POS.raw","Other":"34_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G11", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"35_POS.raw","Other":"35_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G12", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"36_POS.raw","Other":"36_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G13", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"37_POS.raw","Other":"37_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G14", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"38_POS.raw","Other":"38_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G15", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"39_POS.raw","Other":"39_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G16", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"40_POS.raw","Other":"40_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G17", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"41_POS.raw","Other":"41_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"G18", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"42_POS.raw","Other":"42_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N4", "Factors":{"Factor":"POAG"}, "Additional sample data":{"RAW_FILE_NAME":"46_POS.raw","Other":"46_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N1", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"43_POS.raw","Other":"43_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N2", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"44_POS.raw","Other":"44_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N3", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"45_POS.raw","Other":"45_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N5", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"47_POS.raw","Other":"47_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N6", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"48_POS.raw","Other":"48_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N7", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"49_POS.raw","Other":"49_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N8", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"50_POS.raw","Other":"50_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N9", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"51_POS.raw","Other":"51_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N10", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"52_POS.raw","Other":"52_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N11", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"53_POS.raw","Other":"53_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N12", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"54_POS.raw","Other":"54_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N13", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"55_POS.raw","Other":"55_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N14", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"56_POS.raw","Other":"56_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N15", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"57_POS.raw","Other":"57_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N16", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"58_POS.raw","Other":"58_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N17", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"59_POS.raw","Other":"59_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N18", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"60_POS.raw","Other":"60_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N19", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"61_POS.raw","Other":"61_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N20", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"62_POS.raw","Other":"62_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N21", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"63_POS.raw","Other":"63_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N22", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"64_POS.raw","Other":"64_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N23", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"65_POS.raw","Other":"65_NEG.raw"} }, { "Subject ID":"-", "Sample ID":"N24", "Factors":{"Factor":"Control"}, "Additional sample data":{"RAW_FILE_NAME":"66_POS.raw","Other":"66_NEG.raw"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"All AH samples collected came from human donors during cataract surgery. A total of 66 samples were collected which included non-glaucomatous controls and those with pseudoexfoliation syndrome (PEX), pseudoexfoliation glaucoma (PEXG), and primary open-angle glaucoma (POAG). The clinicians provided samples from the Department of Ophthalmology of Hospital Clínico San Carlos in Madrid, Spain. The patient samples consisted of 19 POAG, 9 PEX, 14 PEXG, and 23 controls.","SAMPLE_TYPE":"Eye tissue"}, "TREATMENT":{"TREATMENT_SUMMARY":"No treatment"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Lipids were extracted from POAG, PEX, PEXG, and control AH samples using the Bligh and Dyer method (Bligh and Dyer, 1959). EquiSPLASHTM Lipidomix quantitative mass spec internal standard was spiked into each sample to normalize the lipids. For the extraction, a 2:1 chloroform:methanol solution with 100 µL of water was added to promote phase separation followed by centrifugation at four °C at 14,000 rpm for 20 minutes. After centrifugation, separation was observed and the extracted lipids were collected from the lower, organic chloroform phase with a syringe. Once the lipids were isolated and transferred to 2 mL glass vials, they were dried in a Speed-Vac for about 90 minutes. The upper, aqueous, water/methanol phase containing most of the proteins was collected and stored for a Bradford protein assay to measure protein concentration in the future. When the samples were dehydrated, they were flushed with argon gas for storage at -80°C until ready for mass spec analysis. To prepare the samples for mass spec analysis, the dried lipids were resuspended in a 1:1 acetonitrile:isopropyl alcohol solution, sonicated for 30 minutes, and aliquoted into mass spec vials."}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Vanquish Horizon Binary UHPLC","COLUMN_NAME":"Accucore Vanquish C18+ UHPLC (2.1mm x 150mm x 1.5µm)"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"Lipids were identified and quantified with LipidSearch 4.2.21. Statistical analysis was conducted through MetaboAnalyst 5.0."}, "MS_METABOLITE_DATA":{ "Units":"µg/mL", 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)"},{"metabolite_name":"PC(16:0_18:1)"},{"metabolite_name":"PC(34:0)"},{"metabolite_name":"PC(12:0_22:0)"},{"metabolite_name":"PC(35:0e)"},{"metabolite_name":"PC(34:1D3)"},{"metabolite_name":"PC(36:5e)"},{"metabolite_name":"PC(34:1D7)"},{"metabolite_name":"PC(18:1D7_16:0)"},{"metabolite_name":"PC(16:0e_20:4)"},{"metabolite_name":"PC(36:4e)"},{"metabolite_name":"PC(18:1e_18:2)"},{"metabolite_name":"PC(18:2e_18:1)"},{"metabolite_name":"PC(35:2)"},{"metabolite_name":"PC(18:1e_18:1)"},{"metabolite_name":"PC(35:1)"},{"metabolite_name":"PC(18:0e_18:1)"},{"metabolite_name":"PC(36:5)"},{"metabolite_name":"PC(36:4)"},{"metabolite_name":"PC(16:0_20:4)"},{"metabolite_name":"PC(36:3)"},{"metabolite_name":"PC(18:1_18:2)"},{"metabolite_name":"PC(16:0_20:3)"},{"metabolite_name":"PC(18:1_18:1)"},{"metabolite_name":"PC(18:0_18:2)"},{"metabolite_name":"PC(36:2)"},{"metabolite_name":"PC(37:2e)"},{"metabolite_name":"PC(36:3D3)"},{"metabolite_name":"PC(18:0_18:1)"},{"metabolite_name":"PC(38:6e)"},{"metabolite_name":"PC(18:1e_20:4)"},{"metabolite_name":"PC(38:5e)"},{"metabolite_name":"PC(38:4e)"},{"metabolite_name":"PC(16:0e_22:4)"},{"metabolite_name":"PC(38:2e)"},{"metabolite_name":"PC(38:1e)"},{"metabolite_name":"PC(38:6)"},{"metabolite_name":"PC(18:1_20:4)"},{"metabolite_name":"PC(38:5)"},{"metabolite_name":"PC(38:6D3)"},{"metabolite_name":"PC(38:4)"},{"metabolite_name":"PC(18:0_20:4)"},{"metabolite_name":"PC(38:6D5)"},{"metabolite_name":"PC(38:5D3)"},{"metabolite_name":"PC(38:3)"},{"metabolite_name":"PC(26:0_12:3)"},{"metabolite_name":"PC(38:6D7)"},{"metabolite_name":"PC(38:2)"},{"metabolite_name":"PC(40:8e)"},{"metabolite_name":"PC(40:7e)"},{"metabolite_name":"PC(40:6e)"},{"metabolite_name":"PC(40:5e)"},{"metabolite_name":"PC(40:4e)"},{"metabolite_name":"PC(40:2e)"},{"metabolite_name":"PC(40:7)"},{"metabolite_name":"PC(40:6)"},{"metabolite_name":"PC(40:5)"},{"metabolite_name":"PC(40:4)"},{"metabolite_name":"PC(40:2)"},{"metabolite_name":"PC(40:1)"},{"metabolite_name":"PC(42:5e)"},{"metabolite_name":"PC(18:1e_24:1)"},{"metabolite_name":"PC(42:1e)"},{"metabolite_name":"PC(42:2)"},{"metabolite_name":"PC(44:5e)"},{"metabolite_name":"PC(44:2e)"},{"metabolite_name":"PC(46:2)"},{"metabolite_name":"PC(30:1_18:1)"},{"metabolite_name":"PC(30:0_18:1)"},{"metabolite_name":"PC(50:5)"},{"metabolite_name":"PC(50:2)"},{"metabolite_name":"PC(32:0_18:1)"},{"metabolite_name":"PC(34:1_18:1)"},{"metabolite_name":"PC(54:7)"},{"metabolite_name":"PC(54:5)"},{"metabolite_name":"PC(54:4)"},{"metabolite_name":"PE(24:1D7)"},{"metabolite_name":"PE(26:1D7)"},{"metabolite_name":"PE(27:1D7)"},{"metabolite_name":"PE(30:1D7)"},{"metabolite_name":"PE(32:1e)"},{"metabolite_name":"PE(18:1D7_14:0)"},{"metabolite_name":"PE(32:1D7)"},{"metabolite_name":"PE(34:3e)"},{"metabolite_name":"PE(18:1e_16:1)"},{"metabolite_name":"PE(16:1e_18:1)"},{"metabolite_name":"PE(16:0e_18:1)"},{"metabolite_name":"PE(18:1D7_15:0)"},{"metabolite_name":"PE(35:1e)"},{"metabolite_name":"PE(34:1D7)"},{"metabolite_name":"PE(18:1D7_16:0)"},{"metabolite_name":"PE(36:3e)"},{"metabolite_name":"PE(18:2e_18:1)"},{"metabolite_name":"PE(18:1p_18:1)"},{"metabolite_name":"PE(18:1e_18:1)"},{"metabolite_name":"PE(18:0e_18:1)"},{"metabolite_name":"PE(18:1_18:1)"},{"metabolite_name":"PE(16:0e_22:6)"},{"metabolite_name":"PE(16:0e_22:5)"},{"metabolite_name":"PE(36:1D7)"},{"metabolite_name":"PE(16:0e_22:4)"},{"metabolite_name":"PE(18:1e_20:1)"},{"metabolite_name":"PE(18:1p_22:6)"},{"metabolite_name":"PE(18:1e_22:6)"},{"metabolite_name":"PE(18:1e_22:5)"},{"metabolite_name":"PE(40:5e)"},{"metabolite_name":"PE(18:1e_22:3)"},{"metabolite_name":"PE(40:4e)"},{"metabolite_name":"PE(40:2e)"},{"metabolite_name":"PG(28:0e)"},{"metabolite_name":"PG(15:0_14:0)"},{"metabolite_name":"PG(15:0_15:0)"},{"metabolite_name":"PG(15:0_16:0)"},{"metabolite_name":"PG(18:1D7_15:0)"},{"metabolite_name":"PI(18:1D7_15:0)"},{"metabolite_name":"PI(18:1e_20:4)"},{"metabolite_name":"PS(16:0e_18:1)"},{"metabolite_name":"PS(18:1D7_16:0)"},{"metabolite_name":"PS(36:3e)"},{"metabolite_name":"PS(36:3D7)"},{"metabolite_name":"PS(38:5e)"},{"metabolite_name":"PS(18:1D7_18:0)"},{"metabolite_name":"PS(38:4e)"},{"metabolite_name":"PS(38:3e)"},{"metabolite_name":"PS(38:3D7)"},{"metabolite_name":"PS(40:6e)"},{"metabolite_name":"SM(d28:1)"},{"metabolite_name":"SM(d30:1)"},{"metabolite_name":"SM(d30:0)"},{"metabolite_name":"SM(d31:1)"},{"metabolite_name":"SM(d31:0)"},{"metabolite_name":"SM(d32:2)"},{"metabolite_name":"SM(d18:1_14:0)"},{"metabolite_name":"SM(d32:0)"},{"metabolite_name":"SM(d33:2)"},{"metabolite_name":"SM(d33:1)"},{"metabolite_name":"SM(d33:0)"},{"metabolite_name":"SM(d34:2)"},{"metabolite_name":"SM(d34:1)"},{"metabolite_name":"SM(d17:0_17:1)"},{"metabolite_name":"SM(d34:0)"},{"metabolite_name":"SM(d34:1D3)"},{"metabolite_name":"SM(t33:0)"},{"metabolite_name":"SM(d35:2)"},{"metabolite_name":"SM(d35:1)"},{"metabolite_name":"SM(d35:0)"},{"metabolite_name":"SM(t34:0)"},{"metabolite_name":"SM(d18:1_18:3)"},{"metabolite_name":"SM(d36:2)"},{"m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} }