#METABOLOMICS WORKBENCH engelmab_20231212_234250 DATATRACK_ID:4504 STUDY_ID:ST003011 ANALYSIS_ID:AN004943 PROJECT_ID:PR001874 VERSION 1 CREATED_ON December 13, 2023, 3:06 am #PROJECT PR:PROJECT_TITLE Effects of the human intestinal bacterium Eggerthella lenta in gut of PR:PROJECT_TITLE gnotobiotic mice PR:PROJECT_SUMMARY In this work, we used the minimal consortium Oligo-Mouse Microbiota (OMM)12 to PR:PROJECT_SUMMARY study the function of Coriobacteriia under defined conditions in gnotobiotic PR:PROJECT_SUMMARY mice. OMM12 mice with or without addition of the dominant gut bacterium PR:PROJECT_SUMMARY Eggerthella lenta were fed with diets varying in fat content or supplemented PR:PROJECT_SUMMARY with primary bile acids. PR:INSTITUTE Helmholtz Centre for Environmental Research PR:DEPARTMENT Molecular Systems Biology PR:LAST_NAME Engelmann PR:FIRST_NAME Beatrice PR:ADDRESS Permoserstrasse 15, 04318 Leipzig, Germany PR:EMAIL beatrice.engelmann@ufz.de PR:PHONE 00493411099 #STUDY ST:STUDY_TITLE Untargeted metabolomics analysis of murine colon content ST:STUDY_SUMMARY we used the minimal consortium Oligo-Mouse Microbiota (OMM)12 to study the ST:STUDY_SUMMARY function of Coriobacteriia under defined conditions in gnotobiotic mice. OMM12 ST:STUDY_SUMMARY mice with or without addition of the dominant gut bacterium Eggerthella lenta ST:STUDY_SUMMARY were fed with diets varying in fat content or supplemented with primary bile ST:STUDY_SUMMARY acids. ST:INSTITUTE Helmholtz Centre for Environmental Research ST:DEPARTMENT Molecular Systems Biology ST:LAST_NAME Engelmann ST:FIRST_NAME Beatrice ST:ADDRESS Permoserstrasse 15, 04318 Leipzig, Germany ST:EMAIL beatrice.engelmann@ufz.de ST:PHONE +493411099 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS 2_O_HFD 2_O_HFD Diet:HFD | colonization:OligoMM RAW_FILE_NAME=2_O_HFD.mzML SUBJECT_SAMPLE_FACTORS 3_O_HFD 3_O_HFD Diet:HFD | colonization:OligoMM RAW_FILE_NAME=3_O_HFD.mzML SUBJECT_SAMPLE_FACTORS 4_O_HFD 4_O_HFD Diet:HFD | colonization:OligoMM RAW_FILE_NAME=4_O_HFD.mzML SUBJECT_SAMPLE_FACTORS 5_O_HFD 5_O_HFD Diet:HFD | colonization:OligoMM RAW_FILE_NAME=5_O_HFD.mzML SUBJECT_SAMPLE_FACTORS 6_O_HFD 6_O_HFD Diet:HFD | colonization:OligoMM RAW_FILE_NAME=6_O_HFD.mzML SUBJECT_SAMPLE_FACTORS 7_O_HFD 7_O_HFD Diet:HFD | colonization:OligoMM RAW_FILE_NAME=7_O_HFD.mzML SUBJECT_SAMPLE_FACTORS 8_O_BA 8_O_BA Diet:BA | colonization:OligoMM RAW_FILE_NAME=8_O_BA.mzML SUBJECT_SAMPLE_FACTORS 9_O_HFD.BA 9_O_HFD.BA Diet:HFD.BA | colonization:OligoMM RAW_FILE_NAME=9_O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 11_O_BA 11_O_BA Diet:BA | colonization:OligoMM RAW_FILE_NAME=11_O_BA.mzML SUBJECT_SAMPLE_FACTORS 13_O_CD 13_O_CD Diet:CD | colonization:OligoMM RAW_FILE_NAME=13_O_CD.mzML SUBJECT_SAMPLE_FACTORS 14_O_CD 14_O_CD Diet:CD | colonization:OligoMM RAW_FILE_NAME=14_O_CD.mzML SUBJECT_SAMPLE_FACTORS 17_Corio.O_CD 17_Corio.O_CD Diet:CD | colonization:Corio_OligoMM RAW_FILE_NAME=17_Corio.O_CD.mzML SUBJECT_SAMPLE_FACTORS 19_Corio.O_CD 19_Corio.O_CD Diet:CD | colonization:Corio_OligoMM RAW_FILE_NAME=19_Corio.O_CD.mzML SUBJECT_SAMPLE_FACTORS 20_Corio.O_CD 20_Corio.O_CD Diet:CD | colonization:Corio_OligoMM RAW_FILE_NAME=20_Corio.O_CD.mzML SUBJECT_SAMPLE_FACTORS 21_Corio.O_BA 21_Corio.O_BA Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=21_Corio.O_BA.mzML SUBJECT_SAMPLE_FACTORS 22_Corio.O_BA 22_Corio.O_BA Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=22_Corio.O_BA.mzML SUBJECT_SAMPLE_FACTORS 23_Corio.O_BA 23_Corio.O_BA Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=23_Corio.O_BA.mzML SUBJECT_SAMPLE_FACTORS 24_Corio.O_BA 24_Corio.O_BA Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=24_Corio.O_BA.mzML SUBJECT_SAMPLE_FACTORS 25_Corio.O_HFD 25_Corio.O_HFD Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=25_Corio.O_HFD.mzML SUBJECT_SAMPLE_FACTORS 27_Corio.O_HFD 27_Corio.O_HFD Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=27_Corio.O_HFD.mzML SUBJECT_SAMPLE_FACTORS 28_Corio.O_HFD 28_Corio.O_HFD Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=28_Corio.O_HFD.mzML SUBJECT_SAMPLE_FACTORS 29_Corio.O_HFD.BA 29_Corio.O_HFD.BA Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=29_Corio.O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 30_Corio.O_HFD.BA 30_Corio.O_HFD.BA Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=30_Corio.O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 31_Corio.O_HFD.BA 31_Corio.O_HFD.BA Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=31_Corio.O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 32_Corio.O_HFD.BA 32_Corio.O_HFD.BA Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=32_Corio.O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 33_Corio.O_CD 33_Corio.O_CD Diet:CD | colonization:Corio_OligoMM RAW_FILE_NAME=33_Corio.O_CD.mzML SUBJECT_SAMPLE_FACTORS 34_Corio.O_CD 34_Corio.O_CD Diet:CD | colonization:Corio_OligoMM RAW_FILE_NAME=34_Corio.O_CD.mzML SUBJECT_SAMPLE_FACTORS 35_Corio.O_CD 35_Corio.O_CD Diet:CD | colonization:Corio_OligoMM RAW_FILE_NAME=35_Corio.O_CD.mzML SUBJECT_SAMPLE_FACTORS 36_O_CD 36_O_CD Diet:CD | colonization:OligoMM RAW_FILE_NAME=36_O_CD.mzML SUBJECT_SAMPLE_FACTORS 37_O_CD 37_O_CD Diet:CD | colonization:OligoMM RAW_FILE_NAME=37_O_CD.mzML SUBJECT_SAMPLE_FACTORS 38_O_CD 38_O_CD Diet:CD | colonization:OligoMM RAW_FILE_NAME=38_O_CD.mzML SUBJECT_SAMPLE_FACTORS 39_O_HFD 39_O_HFD Diet:HFD | colonization:OligoMM RAW_FILE_NAME=39_O_HFD.mzML SUBJECT_SAMPLE_FACTORS 40_O_HFD 40_O_HFD Diet:HFD | colonization:OligoMM RAW_FILE_NAME=40_O_HFD.mzML SUBJECT_SAMPLE_FACTORS 41_O_HFD 41_O_HFD Diet:HFD | colonization:OligoMM RAW_FILE_NAME=41_O_HFD.mzML SUBJECT_SAMPLE_FACTORS 42_O_BA 42_O_BA Diet:BA | colonization:OligoMM RAW_FILE_NAME=42_O_BA.mzML SUBJECT_SAMPLE_FACTORS 43_Corio.O_BA 43_Corio.O_BA Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=43_Corio.O_BA.mzML SUBJECT_SAMPLE_FACTORS 44_Corio.O_BA 44_Corio.O_BA Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=44_Corio.O_BA.mzML SUBJECT_SAMPLE_FACTORS 45_Corio.O_BA 45_Corio.O_BA Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=45_Corio.O_BA.mzML SUBJECT_SAMPLE_FACTORS 46_O_CD 46_O_CD Diet:CD | colonization:OligoMM RAW_FILE_NAME=46_O_CD.mzML SUBJECT_SAMPLE_FACTORS 47_Corio.O_HFD.BA 47_Corio.O_HFD.BA Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=47_Corio.O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 48_Corio.O_HFD.BA 48_Corio.O_HFD.BA Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=48_Corio.O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 49_Corio.O_HFD.BA 49_Corio.O_HFD.BA Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=49_Corio.O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 50_Corio.O_HFD 50_Corio.O_HFD Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=50_Corio.O_HFD.mzML SUBJECT_SAMPLE_FACTORS 51_Corio.O_HFD 51_Corio.O_HFD Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=51_Corio.O_HFD.mzML SUBJECT_SAMPLE_FACTORS 52_Corio.O_HFD 52_Corio.O_HFD Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=52_Corio.O_HFD.mzML SUBJECT_SAMPLE_FACTORS 53_Corio.O_HFD 53_Corio.O_HFD Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=53_Corio.O_HFD.mzML SUBJECT_SAMPLE_FACTORS 54_O_BA 54_O_BA Diet:BA | colonization:OligoMM RAW_FILE_NAME=54_O_BA.mzML SUBJECT_SAMPLE_FACTORS 55_O_BA 55_O_BA Diet:BA | colonization:OligoMM RAW_FILE_NAME=55_O_BA.mzML SUBJECT_SAMPLE_FACTORS 56_O_BA 56_O_BA Diet:BA | colonization:OligoMM RAW_FILE_NAME=56_O_BA.mzML SUBJECT_SAMPLE_FACTORS 57_O_BA 57_O_BA Diet:BA | colonization:OligoMM RAW_FILE_NAME=57_O_BA.mzML SUBJECT_SAMPLE_FACTORS 58_O_HFD.BA 58_O_HFD.BA Diet:HFD.BA | colonization:OligoMM RAW_FILE_NAME=58_O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 59_O_HFD.BA 59_O_HFD.BA Diet:HFD.BA | colonization:OligoMM RAW_FILE_NAME=59_O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 60_O_HFD.BA 60_O_HFD.BA Diet:HFD.BA | colonization:OligoMM RAW_FILE_NAME=60_O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 61_O_HFD.BA 61_O_HFD.BA Diet:HFD.BA | colonization:OligoMM RAW_FILE_NAME=61_O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 422_Corio.O_BA 422_Corio.O_BA Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=422_Corio.O_BA.mzML SUBJECT_SAMPLE_FACTORS 462_Corio.O_HFD.BA 462_Corio.O_HFD.BA Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=462_Corio.O_HFD.BA.mzML SUBJECT_SAMPLE_FACTORS 11_6_O_BA 11_6_O_BA Diet:BA | colonization:OligoMM RAW_FILE_NAME=11+6_O_BA.mzML SUBJECT_SAMPLE_FACTORS 2_O_HFD 2_O_HFD_pos Diet:HFD | colonization:OligoMM RAW_FILE_NAME=2_O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 3_O_HFD 3_O_HFD_pos Diet:HFD | colonization:OligoMM RAW_FILE_NAME=3_O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 4_O_HFD 4_O_HFD_pos Diet:HFD | colonization:OligoMM RAW_FILE_NAME=4_O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 5_O_HFD 5_O_HFD_pos Diet:HFD | colonization:OligoMM RAW_FILE_NAME=5_O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 6_O_HFD 6_O_HFD_pos Diet:HFD | colonization:OligoMM RAW_FILE_NAME=6_O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 7_O_HFD 7_O_HFD_pos Diet:HFD | colonization:OligoMM RAW_FILE_NAME=7_O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 8_O_BA 8_O_BA_pos Diet:BA | colonization:OligoMM RAW_FILE_NAME=8_O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 9_O_HFD.BA 9_O_HFD.BA_pos Diet:HFD.BA | colonization:OligoMM RAW_FILE_NAME=9_O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 11_O_BA 11_O_BA_pos Diet:BA | colonization:OligoMM RAW_FILE_NAME=11_O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 13_O_CD 13_O_CD_pos Diet:CD | colonization:OligoMM RAW_FILE_NAME=13_O_CD_pos.mzML SUBJECT_SAMPLE_FACTORS 14_O_CD 14_O_CD_pos Diet:CD | colonization:OligoMM RAW_FILE_NAME=14_O_CD_pos.mzML SUBJECT_SAMPLE_FACTORS 17_Corio.O_CD 17_Corio.O_CD_pos Diet:CD | colonization:Corio_OligoMM RAW_FILE_NAME=17_Corio.O_CD_pos.mzML SUBJECT_SAMPLE_FACTORS 19_Corio.O_CD 19_Corio.O_CD_pos Diet:CD | colonization:Corio_OligoMM RAW_FILE_NAME=19_Corio.O_CD_pos.mzML SUBJECT_SAMPLE_FACTORS 20_Corio.O_CD 20_Corio.O_CD_pos Diet:CD | colonization:Corio_OligoMM RAW_FILE_NAME=20_Corio.O_CD_pos.mzML SUBJECT_SAMPLE_FACTORS 21_Corio.O_BA 21_Corio.O_BA_pos Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=21_Corio.O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 22_Corio.O_BA 22_Corio.O_BA_pos Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=22_Corio.O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 23_Corio.O_BA 23_Corio.O_BA_pos Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=23_Corio.O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 24_Corio.O_BA 24_Corio.O_BA_pos Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=24_Corio.O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 25_Corio.O_HFD 25_Corio.O_HFD_pos Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=25_Corio.O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 27_Corio.O_HFD 27_Corio.O_HFD_pos Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=27_Corio.O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 28_Corio.O_HFD 28_Corio.O_HFD_pos Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=28_Corio.O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 29_Corio.O_HFD.BA 29_Corio.O_HFD.BA_pos Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=29_Corio.O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 30_Corio.O_HFD.BA 30_Corio.O_HFD.BA_pos Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=30_Corio.O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 31_Corio.O_HFD.BA 31_Corio.O_HFD.BA_pos Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=31_Corio.O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 32_Corio.O_HFD.BA 32_Corio.O_HFD.BA_pos Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=32_Corio.O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 33_Corio.O_CD 33_Corio.O_CD_pos Diet:CD | colonization:Corio_OligoMM RAW_FILE_NAME=33_Corio.O_CD_pos.mzML SUBJECT_SAMPLE_FACTORS 34_Corio.O_CD 34_Corio.O_CD_pos Diet:CD | colonization:Corio_OligoMM RAW_FILE_NAME=34_Corio.O_CD_pos.mzML SUBJECT_SAMPLE_FACTORS 35_Corio.O_CD 35_Corio.O_CD_pos Diet:CD | colonization:Corio_OligoMM RAW_FILE_NAME=35_Corio.O_CD_pos.mzML SUBJECT_SAMPLE_FACTORS 36_O_CD 36_O_CD_pos Diet:CD | colonization:OligoMM RAW_FILE_NAME=36_O_CD_pos.mzML SUBJECT_SAMPLE_FACTORS 37_O_CD 37_O_CD_pos Diet:CD | colonization:OligoMM RAW_FILE_NAME=37_O_CD_pos.mzML SUBJECT_SAMPLE_FACTORS 38_O_CD 38_O_CD_pos Diet:CD | colonization:OligoMM RAW_FILE_NAME=38_O_CD_pos.mzML SUBJECT_SAMPLE_FACTORS 39_O_HFD 39_O_HFD_pos Diet:HFD | colonization:OligoMM RAW_FILE_NAME=39_O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 40_O_HFD 40_O_HFD_pos Diet:HFD | colonization:OligoMM RAW_FILE_NAME=40_O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 41_O_HFD 41_O_HFD_pos Diet:HFD | colonization:OligoMM RAW_FILE_NAME=41_O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 42_O_BA 42_O_BA_pos Diet:BA | colonization:OligoMM RAW_FILE_NAME=42_O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 43_Corio.O_BA 43_Corio.O_BA_pos Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=43_Corio.O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 44_Corio.O_BA 44_Corio.O_BA_pos Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=44_Corio.O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 45_Corio.O_BA 45_Corio.O_BA_pos Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=45_Corio.O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 46_O_CD 46_O_CD_pos Diet:CD | colonization:OligoMM RAW_FILE_NAME=46_O_CD_pos.mzML SUBJECT_SAMPLE_FACTORS 47_Corio.O_HFD.BA 47_Corio.O_HFD.BA_pos Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=47_Corio.O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 48_Corio.O_HFD.BA 48_Corio.O_HFD.BA_pos Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=48_Corio.O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 49_Corio.O_HFD.BA 49_Corio.O_HFD.BA_pos Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=49_Corio.O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 50_Corio.O_HFD 50_Corio.O_HFD_pos Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=50_Corio.O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 51_Corio.O_HFD 51_Corio.O_HFD_pos Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=51_Corio.O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 52_Corio.O_HFD 52_Corio.O_HFD_pos Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=52_Corio.O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 53_Corio.O_HFD 53_Corio.O_HFD_pos Diet:HFD | colonization:Corio_OligoMM RAW_FILE_NAME=53_Corio.O_HFD_pos.mzML SUBJECT_SAMPLE_FACTORS 54_O_BA 54_O_BA_pos Diet:BA | colonization:OligoMM RAW_FILE_NAME=54_O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 55_O_BA 55_O_BA_pos Diet:BA | colonization:OligoMM RAW_FILE_NAME=55_O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 56_O_BA 56_O_BA_pos Diet:BA | colonization:OligoMM RAW_FILE_NAME=56_O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 57_O_BA 57_O_BA_pos Diet:BA | colonization:OligoMM RAW_FILE_NAME=57_O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 58_O_HFD.BA 58_O_HFD.BA_pos Diet:HFD.BA | colonization:OligoMM RAW_FILE_NAME=58_O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 59_O_HFD.BA 59_O_HFD.BA_pos Diet:HFD.BA | colonization:OligoMM RAW_FILE_NAME=59_O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 60_O_HFD.BA 60_O_HFD.BA_pos Diet:HFD.BA | colonization:OligoMM RAW_FILE_NAME=60_O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 61_O_HFD.BA 61_O_HFD.BA_pos Diet:HFD.BA | colonization:OligoMM RAW_FILE_NAME=61_O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 422_Corio.O_BA 422_Corio.O_BA_pos Diet:BA | colonization:Corio_OligoMM RAW_FILE_NAME=422_Corio.O_BA_pos.mzML SUBJECT_SAMPLE_FACTORS 462_Corio.O_HFD.BA 462_Corio.O_HFD.BA_pos Diet:HFD.BA | colonization:Corio_OligoMM RAW_FILE_NAME=462_Corio.O_HFD.BA_pos.mzML SUBJECT_SAMPLE_FACTORS 11_6_O_BA 11_6_O_BA_pos Diet:BA | colonization:OligoMM RAW_FILE_NAME=11+6_O_BA_pos.mzML #COLLECTION CO:COLLECTION_SUMMARY Mice were euthanized with CO2 and colon was extracted. CO:SAMPLE_TYPE Colon #TREATMENT TR:TREATMENT_SUMMARY Germfree male C57BL/6N mice were associated with 0.2 ml of the freshly thawed TR:TREATMENT_SUMMARY microbial mixtures via oral gavage three times within one week at the age of TR:TREATMENT_SUMMARY five weeks. From week 8 onwards, diet was changed from autoclaved standard chow TR:TREATMENT_SUMMARY to synthetic control diet (CD) for two weeks. Thereafter, the mice were randomly TR:TREATMENT_SUMMARY assigned to four different groups fed the following diets for eight weeks (the TR:TREATMENT_SUMMARY references and main differences in composition between the diets are provided in TR:TREATMENT_SUMMARY Figure S1B): CD, control diet; CD-BA, control diet supplemented with the primary TR:TREATMENT_SUMMARY bile acids cholic acid (CA) and chenodeoxycholic acid (CDCA) (each 0.1%); HFD, TR:TREATMENT_SUMMARY lard-based high-fat diet (48% energy from fat); HFD-BA, HFD supplemented with CA TR:TREATMENT_SUMMARY and CDCA. These diets were used due to the link between Coriobacteriia/E. lenta TR:TREATMENT_SUMMARY and bile acid metabolism as well as host metabolic health. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Colon content was mixed with 5x ACN:H2O solvent and 4 steel beads for subsequent SP:SAMPLEPREP_SUMMARY extraction in the bead mill. After centrifugation, 100 µL of the supernatant SP:SAMPLEPREP_SUMMARY were mixed with 500 µL methanol:acetonitrile (ACN):water (2:3:1) followed by SP:SAMPLEPREP_SUMMARY vortex and sonication. After centrifugation, 100 µL of the supernatant were SP:SAMPLEPREP_SUMMARY dried in a SpeedVac™ vacuum concentrator (Eppendorf, Hamburg, Germany). #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1290 Infinity II CH:COLUMN_NAME Agilent ZORBAX Eclipse Plus C18 (100 x 2.1mm,1.8um) CH:SOLVENT_A Water + 0.1% FA CH:SOLVENT_B ACN + 0.1% FA CH:FLOW_GRADIENT 0-5.5 min: 1% B; 5-20 min: 1-100% B; 20-22 min: 100% B; 22-22.5: 100-1% B; CH:FLOW_GRADIENT 22.5-25 min: 1% B CH:FLOW_RATE 0.3 ml/min CH:COLUMN_TEMPERATURE 45 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent 6546 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS All samples were acquired in positive and negative ionization mode. The QTOF was MS:MS_COMMENTS set up in centroid mode with a scan range of 50 – 1000 m/z. After each full MS:MS_COMMENTS scan, the 2 most intense ions were subjected to fragmentation. The obtained raw MS:MS_COMMENTS data (.d-files) were imported into the Progenesis QI® software (version2.4, MS:MS_COMMENTS Waters Corporation) for further analysis. MS:MS_RESULTS_FILE ST003011_AN004943_Results.txt UNITS:Peak AUC Has m/z:Yes Has RT:Yes RT units:Minutes #END