{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003046","ANALYSIS_ID":"AN004997","VERSION":"1","CREATED_ON":"January 18, 2024, 3:40 pm"},

"PROJECT":{"PROJECT_TITLE":"Multi-Omics Plasma Signatures of Severe Injury with Influence of REBOA Intervention in a Swine Model.","PROJECT_TYPE":"Origninal Research","PROJECT_SUMMARY":"Swine experienced controlled severe injury involving combinations of trauma, hemorrhagic shock, and disseminated complex blast injury (DCBI). After a period of hemorrhagic shock, resuscitation was performed using REBOA and shed blood transfusion. Blood samples were collected from sham and experiment swine. Blood samples for experiment swine were collected at baseline and periodically through the monitored time course. The plasma fraction were then subjected to mass spectrometry based metabolomics.","INSTITUTE":"1) Department of Biochemistry and Molecular Genetics, University of Colorado Denver – Anschutz Medical Campus, Aurora, CO, USA","LABORATORY":"The D''Alessandro Lab","LAST_NAME":"LaCroix","FIRST_NAME":"Ian","ADDRESS":"12801 E 17th Ave, Aurora, Colorado, 80045, USA","EMAIL":"ian.lacroix@cuanschutz.edu","PHONE":"(303) 724-0096","PUBLICATIONS":"DOI: 10.1097/SLA.0000000000005944"},

"STUDY":{"STUDY_TITLE":"Multi-Omics Plasma Signatures of Severe Injury with Influence of REBOA Intervention in a Swine Model.","STUDY_TYPE":"Original Research","STUDY_SUMMARY":"Swine experienced controlled severe injury involving combinations of trauma, hemorrhagic shock, and disseminated complex blast injury (DCBI). After a period of hemorrhagic shock, resuscitation was performed using Resuscitative Endovascular Balloon Occlusion of the Aorta (REBOA) and shed blood transfusion. Blood samples were collected from sham and experiment swine. Blood samples for experiment swine were collected at baseline and periodically through the monitored time course. The plasma fraction were then subjected to mass spectrometry based metabolomics. Results indicated intervention using REBOA following polytrauma in our swine model induced distinct multi-omics alterations as a function of placement location. Namely, aortic balloon placement in Zone-1 worsened ischemic damage and mitochondrial dysfunction, patterns that continued through the monitored time course.","INSTITUTE":"University of Colorado Anschutz Medical Campus","LABORATORY":"The D''Alessandro Lab","LAST_NAME":"LaCroix","FIRST_NAME":"Ian","ADDRESS":"RC1-S L18-9403G E 17th Ave. Aurora, CO 80045","EMAIL":"ian.lacroix@cuanschutz.edu","PHONE":"(303) 724-0096"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Sus scrofa","TAXONOMY_ID":"9823","GENDER":"Male"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"1",
"Sample ID":"1",
"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-001"}
},
{
"Subject ID":"1",
"Sample ID":"2",
"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-002"}
},
{
"Subject ID":"1",
"Sample ID":"3",
"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-003"}
},
{
"Subject ID":"1",
"Sample ID":"4",
"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-004"}
},
{
"Subject ID":"1",
"Sample ID":"5",
"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-005"}
},
{
"Subject ID":"1",
"Sample ID":"6",
"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"180"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-006"}
},
{
"Subject ID":"1",
"Sample ID":"7",
"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"240"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-007"}
},
{
"Subject ID":"2",
"Sample ID":"8",
"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-008"}
},
{
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"Sample ID":"9",
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-009"}
},
{
"Subject ID":"2",
"Sample ID":"10",
"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-010"}
},
{
"Subject ID":"2",
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"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-011"}
},
{
"Subject ID":"2",
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"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-012"}
},
{
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-013"}
},
{
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"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"240"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-014"}
},
{
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-015"}
},
{
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"Sample ID":"16",
"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-016"}
},
{
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"Sample ID":"17",
"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-017"}
},
{
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"Factors":{"Pig_Model":"HS","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-018"}
},
{
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-019"}
},
{
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-020"}
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-023"}
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-032"}
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-037"}
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-038"}
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-042"}
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-043"}
},
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-044"}
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-045"}
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-049"}
},
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-050"}
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-051"}
},
{
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"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-052"}
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"Factors":{"Pig_Model":"TI + HS","Time_from_End_of_Shock_min":"0"},
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},
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},
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},
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"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-057"}
},
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"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-058"}
},
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"Subject ID":"10",
"Sample ID":"59",
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"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-059"}
},
{
"Subject ID":"10",
"Sample ID":"60",
"Factors":{"Pig_Model":"TI + HS","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-060"}
},
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"Subject ID":"10",
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"Factors":{"Pig_Model":"TI + HS","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-061"}
},
{
"Subject ID":"11",
"Sample ID":"62",
"Factors":{"Pig_Model":"TI + HS","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-062"}
},
{
"Subject ID":"11",
"Sample ID":"63",
"Factors":{"Pig_Model":"TI + HS","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-063"}
},
{
"Subject ID":"11",
"Sample ID":"64",
"Factors":{"Pig_Model":"TI + HS","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-064"}
},
{
"Subject ID":"11",
"Sample ID":"65",
"Factors":{"Pig_Model":"TI + HS","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-065"}
},
{
"Subject ID":"11",
"Sample ID":"66",
"Factors":{"Pig_Model":"TI + HS","Time_from_End_of_Shock_min":"120"},
"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-066"}
},
{
"Subject ID":"11",
"Sample ID":"67",
"Factors":{"Pig_Model":"TI + HS","Time_from_End_of_Shock_min":"180"},
"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-067"}
},
{
"Subject ID":"11",
"Sample ID":"68",
"Factors":{"Pig_Model":"TI + HS","Time_from_End_of_Shock_min":"240"},
"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-068"}
},
{
"Subject ID":"13",
"Sample ID":"69",
"Factors":{"Pig_Model":"DCBI","Time_from_End_of_Shock_min":"NA"},
"Additional sample data":{"Vendor":"NA","Breed":"NA","RAW_FILE_NAME":"ILC03-069"}
},
{
"Subject ID":"13",
"Sample ID":"70",
"Factors":{"Pig_Model":"DCBI","Time_from_End_of_Shock_min":"NA"},
"Additional sample data":{"Vendor":"NA","Breed":"NA","RAW_FILE_NAME":"ILC03-070"}
},
{
"Subject ID":"13",
"Sample ID":"71",
"Factors":{"Pig_Model":"DCBI","Time_from_End_of_Shock_min":"NA"},
"Additional sample data":{"Vendor":"NA","Breed":"NA","RAW_FILE_NAME":"ILC03-071"}
},
{
"Subject ID":"14",
"Sample ID":"73",
"Factors":{"Pig_Model":"TXA + DCBI","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-073"}
},
{
"Subject ID":"14",
"Sample ID":"74",
"Factors":{"Pig_Model":"TXA + DCBI","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-074"}
},
{
"Subject ID":"14",
"Sample ID":"75",
"Factors":{"Pig_Model":"TXA + DCBI","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-075"}
},
{
"Subject ID":"14",
"Sample ID":"76",
"Factors":{"Pig_Model":"TXA + DCBI","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-076"}
},
{
"Subject ID":"15",
"Sample ID":"77",
"Factors":{"Pig_Model":"DCBI","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-077"}
},
{
"Subject ID":"15",
"Sample ID":"78",
"Factors":{"Pig_Model":"DCBI","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-078"}
},
{
"Subject ID":"15",
"Sample ID":"79",
"Factors":{"Pig_Model":"DCBI","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-079"}
},
{
"Subject ID":"15",
"Sample ID":"80",
"Factors":{"Pig_Model":"DCBI","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-080"}
},
{
"Subject ID":"15",
"Sample ID":"81",
"Factors":{"Pig_Model":"DCBI","Time_from_End_of_Shock_min":"120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-081"}
},
{
"Subject ID":"15",
"Sample ID":"82",
"Factors":{"Pig_Model":"DCBI","Time_from_End_of_Shock_min":"180"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-082"}
},
{
"Subject ID":"15",
"Sample ID":"83",
"Factors":{"Pig_Model":"DCBI","Time_from_End_of_Shock_min":"240"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-083"}
},
{
"Subject ID":"16",
"Sample ID":"84",
"Factors":{"Pig_Model":"TXA + DCBI","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-084"}
},
{
"Subject ID":"16",
"Sample ID":"85",
"Factors":{"Pig_Model":"TXA + DCBI","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-085"}
},
{
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"Factors":{"Pig_Model":"TI","Time_from_End_of_Shock_min":"120"},
"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-272"}
},
{
"Subject ID":"48",
"Sample ID":"273",
"Factors":{"Pig_Model":"TI","Time_from_End_of_Shock_min":"180"},
"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-273"}
},
{
"Subject ID":"48",
"Sample ID":"274",
"Factors":{"Pig_Model":"TI","Time_from_End_of_Shock_min":"240"},
"Additional sample data":{"Vendor":"Midwest Research Swine","Breed":"American Yorkshire-Landrace-Duroc","RAW_FILE_NAME":"ILC03-274"}
},
{
"Subject ID":"49",
"Sample ID":"275",
"Factors":{"Pig_Model":"-","Time_from_End_of_Shock_min":"NA"},
"Additional sample data":{"Vendor":"NA","Breed":"NA","RAW_FILE_NAME":"ILC03-275"}
},
{
"Subject ID":"49",
"Sample ID":"276",
"Factors":{"Pig_Model":"-","Time_from_End_of_Shock_min":"NA"},
"Additional sample data":{"Vendor":"NA","Breed":"NA","RAW_FILE_NAME":"ILC03-276"}
},
{
"Subject ID":"50",
"Sample ID":"279",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-279"}
},
{
"Subject ID":"50",
"Sample ID":"280",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-280"}
},
{
"Subject ID":"50",
"Sample ID":"281",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-281"}
},
{
"Subject ID":"50",
"Sample ID":"282",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-282"}
},
{
"Subject ID":"50",
"Sample ID":"283",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-283"}
},
{
"Subject ID":"50",
"Sample ID":"284",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"180"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-284"}
},
{
"Subject ID":"50",
"Sample ID":"285",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"240"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-285"}
},
{
"Subject ID":"51",
"Sample ID":"286",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-286"}
},
{
"Subject ID":"52",
"Sample ID":"288",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-288"}
},
{
"Subject ID":"52",
"Sample ID":"289",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-289"}
},
{
"Subject ID":"52",
"Sample ID":"290",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-290"}
},
{
"Subject ID":"52",
"Sample ID":"291",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-291"}
},
{
"Subject ID":"52",
"Sample ID":"292",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-292"}
},
{
"Subject ID":"52",
"Sample ID":"293",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"180"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-293"}
},
{
"Subject ID":"52",
"Sample ID":"294",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"240"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-294"}
},
{
"Subject ID":"53",
"Sample ID":"295",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-295"}
},
{
"Subject ID":"53",
"Sample ID":"296",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-296"}
},
{
"Subject ID":"53",
"Sample ID":"297",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-297"}
},
{
"Subject ID":"53",
"Sample ID":"298",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-298"}
},
{
"Subject ID":"53",
"Sample ID":"299",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-299"}
},
{
"Subject ID":"53",
"Sample ID":"300",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"180"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-300"}
},
{
"Subject ID":"53",
"Sample ID":"301",
"Factors":{"Pig_Model":"TXA + Zone 1","Time_from_End_of_Shock_min":"240"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-301"}
},
{
"Subject ID":"54",
"Sample ID":"302",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-302"}
},
{
"Subject ID":"54",
"Sample ID":"303",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-303"}
},
{
"Subject ID":"54",
"Sample ID":"304",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-304"}
},
{
"Subject ID":"54",
"Sample ID":"305",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-305"}
},
{
"Subject ID":"54",
"Sample ID":"306",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-306"}
},
{
"Subject ID":"54",
"Sample ID":"307",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"180"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-307"}
},
{
"Subject ID":"54",
"Sample ID":"308",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"240"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-308"}
},
{
"Subject ID":"55",
"Sample ID":"309",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-309"}
},
{
"Subject ID":"55",
"Sample ID":"310",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-310"}
},
{
"Subject ID":"55",
"Sample ID":"311",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-311"}
},
{
"Subject ID":"55",
"Sample ID":"312",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-312"}
},
{
"Subject ID":"55",
"Sample ID":"313",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-313"}
},
{
"Subject ID":"55",
"Sample ID":"314",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"180"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-314"}
},
{
"Subject ID":"55",
"Sample ID":"315",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"240"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-315"}
},
{
"Subject ID":"56",
"Sample ID":"316",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-316"}
},
{
"Subject ID":"56",
"Sample ID":"317",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-317"}
},
{
"Subject ID":"56",
"Sample ID":"318",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-318"}
},
{
"Subject ID":"56",
"Sample ID":"319",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-319"}
},
{
"Subject ID":"56",
"Sample ID":"320",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-320"}
},
{
"Subject ID":"56",
"Sample ID":"321",
"Factors":{"Pig_Model":"TXA + Zone 3","Time_from_End_of_Shock_min":"180"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-321"}
},
{
"Subject ID":"57",
"Sample ID":"323",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-323"}
},
{
"Subject ID":"57",
"Sample ID":"324",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-324"}
},
{
"Subject ID":"57",
"Sample ID":"325",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-325"}
},
{
"Subject ID":"58",
"Sample ID":"327",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-327"}
},
{
"Subject ID":"58",
"Sample ID":"328",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-328"}
},
{
"Subject ID":"58",
"Sample ID":"329",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-329"}
},
{
"Subject ID":"58",
"Sample ID":"330",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-330"}
},
{
"Subject ID":"58",
"Sample ID":"331",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-331"}
},
{
"Subject ID":"58",
"Sample ID":"332",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"180"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-332"}
},
{
"Subject ID":"58",
"Sample ID":"333",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"240"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-333"}
},
{
"Subject ID":"59",
"Sample ID":"334",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"-120"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-334"}
},
{
"Subject ID":"59",
"Sample ID":"335",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"0"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-335"}
},
{
"Subject ID":"59",
"Sample ID":"336",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"30"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-336"}
},
{
"Subject ID":"59",
"Sample ID":"337",
"Factors":{"Pig_Model":"REBOA Zone III + DCBI","Time_from_End_of_Shock_min":"60"},
"Additional sample data":{"Vendor":"Oak Hills Genetics","Breed":"White Yorkshire x Landrace","RAW_FILE_NAME":"ILC03-337"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Healthy adolescent, male Yorkshire swine (average weight 50.0 ± 2.7 kg) were acclimated in the animal facilities for a minimum of 3 days and examined by a veterinarian on the day of experimentation. In brief, on the day of surgery, swine anesthesia was induced via intramuscular injection of ketamine (20.0 mg/kg), xylazine (2.0 mg/kg), and acepromazine (0.2 mg/kg) followed by intubation under direct laryngoscopy. Continuous infusion anesthesia was maintained through a superficial ear vein using propofol (3 mg/kg/h) and fentanyl (3 mcg/kg/h). Lactated Ringer’s solution was administered at a rate of 10 mL/kg/h to overcome insensible losses. Mahurkar catheters (12 Fr) were placed in the femoral arteries and veins for vascular access.","COLLECTION_PROTOCOL_ID":"Animal Care and Use Committee approved this animal study under protocol #1050","SAMPLE_TYPE":"Blood (plasma)","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"the DCBI model included blast traumatic brain injury (bTBI), tissue injury through bilateral open femur fractures, followed by severe fixed pressure targeted hemorrhagic shock (HS). Zone-I aortic occlusion (AO) was performed using the ER-REBOA Catheter (Prytime Medical Devices, Inc., Boerne, TX). The catheter was inserted through a 7 Fr sheath placed in the common femoral artery, was advanced to 50 cm, and inflated using 6 mL of normal saline for Zone-I AO and placed at 26 cm with 5 ml of saline for Zone-III, and both were maintained for the final 30 minutes of the HS phase."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Plasma samples were thawed on ice then 20 μl was aliquoted into individual 1.5mL microcentrifuge tubes. The plasma was diluted to 480 μl with methanol:acetonitrile:water (5:3:2, v/v/v). The metabolite containing supernatant was transferred to individual auto sampler vials on ice.","SAMPLEPREP_PROTOCOL_COMMENTS":"DOI https://doi.org/10.1007/978-1-4939-9236-2_2","PROCESSING_STORAGE_CONDITIONS":"-80℃","EXTRACT_STORAGE":"-80℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"The LC-MS solvents A and B, as well as the Flow gradient details are proprietary and will be made available upon request (angelo.dalesandro@cuanschutz.edu)","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Phenomenex Kinetex C18 (150 x 2.1mm,1.7um)","SOLVENT_A":"-","SOLVENT_B":"-","FLOW_GRADIENT":"-","FLOW_RATE":"0.3mL/min","COLUMN_TEMPERATURE":"45"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Scanning in Full MS mode (2 μscans) from 90 to 900 m/z at 70,000 resolution, with 4 kV spray voltage, 45 sheath gas, 15 auxiliary gas(1). Following data acquisition, .raw files were converted to .mzXML using RawConverter then metabolites assigned and peaks integrated using ElMaven (Elucidata) in conjunction with an in-house standard library."},

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}

}